Kamal Ahmed Mohamed Abo-Elyousr
@kau.edu.sa
Faculty of Meteorology, Environment and Arid Land Agriculture
KING ABDULAZIZ UNIVERSITY
RESEARCH INTERESTS
Plant Pathology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Mohamed Hassan Abd El-Wahed, Najeeb M. Almasoudi, Kamal A.M. Abo-Elyousr, and Hadeel M.M. Khalil Bagy
Springer Science and Business Media LLC
Kamal A. M. Abo-Elyousr, Nashwa M.A Sallam, Magdy A. A Mousa, Muhammad Imran, and Ismail R. Abdel-Rahim
Springer Science and Business Media LLC
Mohamed A. A. Seleim, Mohamed F. F. Bereika, Omer H. M. Ibrahim, Ahmed I. Alqubaie, and Kamal A. M. Abo-Elyousr
Springer Science and Business Media LLC
Sherif A. Ahmed, Sameer A. Nagadi, Kamal A. M. Abo-Elyousr, and Mansour M. El-Fawy
Springer Science and Business Media LLC
Sherif A. Ahmed, Sameer A. Nagadi, Kamal A. M. Abo-Elyousr, and Mansour M. El-Fawy
Springer Science and Business Media LLC
Mohamed A. M. Hussein, Ahmed M. K. Abdel-Aal, Muhyaddin J. Rawa, Magdi A. A. Mousa, Yasser M. M. Moustafa, and Kamal A. M. Abo-Elyousr
Springer Science and Business Media LLC
Abstract Background Leveillula taurica is an obligate pathogen that causes powdery mildew on chili pepper (Capsicum annuum L.) which is considered one of the most serious diseases for the crop. Results Leveillula taurica was isolated from infected pepper plants in Assiut Governorate, Egypt. Pathogenicity test was performed, and it was found that the pathogen can cause the symptoms of powdery mildew on the pepper plant. Under greenhouse conditions, treatment with Bacillus thuringiensis MW740161.1, Pseudomonas fluorescens, and Bacillus subtilis cultures resulted in a significant reduction in conidial germination of the pathogen (69.07, 29.55, and 19.58%, respectively). Spraying chili pepper plants with the microorganisms effectively reduced the powdery mildew's disease severity. Also, treatment with the bacterial strains resulted in a significant (P 0.05%) increase in the yield of chili pepper. Based on the findings, it appears that the use of B. thuringiensis, as foliar spraying, significantly induced resistance of chili pepper plants against L. taurica and stimulated many biochemical functions in the plant. Also, it increased the crop yield compared to all other treatments. Conclusions This study recommends B. thuringiensis as a viable alternative to harmful pesticides, and it is feasible to formulate an appropriate fungicide for the sustainable green production of chili peppers. The B. thuringiensis can increase the resistance of chili pepper plant to L. taurica the causal pathogen of powdery mildew.
Mansour M. El-Fawy, Kamal A. M. Abo-Elyousr, Sherif A. Ahmed, Reda A. A. Korrat, and Ayman S. Saeed
Springer Science and Business Media LLC
Mohamed Hassan Abd El-Wahed, Mohamed F. F. Bereika, Kamal A. M. Abo-Elyousr, and Najeeb Marei Almasoudi
Springer Science and Business Media LLC
Abstract Background Bacterial wilt caused by Ralstonia solanacearum is considered one of the most important diseases that cause economic losses to many economic crops and spread in many regions around the world, especially in the subtropical tropics and some warm temperate regions of the world. In this study the aqueous plant extract of Rosemarinus officinalis and Pseudomonas fluorescens for their antagonistic activity against bacterial wilt in vitro, greenhouse and population of R, solanacearum in plants was study. Results Following intensive screening studies, aqueous plant extract of R. officinalis and P. fluorescens was selected to study their effectiveness against bacterial wilt of potato plants and their antimicrobial activity or induction of systemic resistance in plants. In greenhouse, all soil drenching treatments on potato plants significantly reduced disease incidence of potato bacterial wilt than infected control. R. officinalis, P. fluorescens mixture between plant extract and endophytic bacteria and streptomycin reduced profoundly the disease severity by 75.51, 65.0 77.9 and 81.00%, respectively, than the infected control (83.71%). The plant extract and microorganism significantly increased fresh and dry weight of potato plants (g) per plant compared to non-treated control plants. R. officinalis, P. fluorescens mixture between plant extract and endophytic bacterium and streptomycin increased fresh weight from 100.1 to 169.9 compared to infected control also increased dry weight from 68.4 in streptomycin to 170.4% in mixture of plant extract and endophytic bacterium. The populations of bacterial pathogen R. solanacearum were lowest in stem of potato plants treated with plant extracts than in inoculated control plants (50%). In general, the total phenols increased in both inoculated and non-inoculated potato plants. Conclusions Ralstonia officinalis and P. fluorescens showed a strong in vitro activity in relation growth limitation of R. solanacearum as well as limiting the development of bacterial wilt disease on potato plants under greenhouse conditions.
Hadeel M. M. Kalil Bagy, Kamal A. M. Abo-Elyousr, Abd El-Latif Hesham, and Nashwa M. A. Sallam
Springer Science and Business Media LLC
Abstract Background The present study aimed to examine the biocontrol efficacy of 28 yeast isolates against Aspergillus niger, the common pathogen of onion black mold disease. The antagonistic potential of yeast isolates against A. niger growth was investigated using a dual culture technique. Results Five yeast isolates, including 8 and 11, showed the highest inhibition effect on the mycelial growth of A. niger. Molecular analysis using 16S RNA identified strains 8 (AUN-AH14) and 11 (AUN-AH23) as Galactomyces geotrichum (JQ713185.1) and G. geotrichum (DQ849321.1), respectively. The culture filtrates of AUN-AH14 and AUN-AH23 at percentage concentrations of 20, 40, 60, and 80 were tested against the growth of A. niger. As a result, the lowest dry weight of the pathogen was obtained with culture filtrates of the two strains at concentrations of 60 and 80%.. Treatment of onion bulbs with formulation of antagonistic yeast isolates AUN-AH14 and AUN-AH23 and their corresponding cultural filtrates at 80% concentration significantly reduced the severity of black mold disease relative to the controls. Conclusions The most significant reduction in black mold severity was proved in yeast formulations of AUN-AH14 and AUN-AH23, followed by their cultural filtrates (CF-AUN-AH14 and CF-AUN-AH23). The shelf life of formulated yeast isolates began to gradually deteriorate after 5 months of storage at 4 °C.
Eva M. Achata, Magdi A.A. Mousa, Adel D. Al-Qurashi, Omer H.M. Ibrahim, Kamal A.M. Abo-Elyousr, Ahmed M.K. Abdel Aal, and Mohammed Kamruzzaman
Elsevier BV
Kamal A. M. Abo-Elyousr, Magdi A. A. Mousa, and Omer H. M. Ibrahim
Springer Science and Business Media LLC
Suleiman K. Bello, Samir G. AL-Solaimani, and Kamal A. M. Abo-Elyousr
Springer Science and Business Media LLC
Magdi A. A. Mousa, Kamal A. M. Abo-Elyousr, and Omer H. M. Ibrahim
MDPI AG
The availability of adequate information about the documentation and characterization of germplasm is fundamental for any crop improvement program. The importance of cumin as a medicinal plant yet the lack of information about its genetic variability encouraged us to initiate the current study aiming at assessing the genetic variability among 17 cumin genotypes from different geographical regions using four molecular markers (RAPD, ISSR, SRAP and SCoT). Further, the potential of six accessions to induce callus was studied under in vitro conditions on MS and B5 basal media supplemented with various combinations between 2,4-D and kinetin. Our findings showed that combining 87 primers, including 42, 15, 7 and 23 primers of RAPD, ISSR, SCoT and SRAP, respectively, facilitated detecting the relationship among the assessed cumin accessions. A total number of 765 bands were analyzed, among which only 74 bands were polymorphic. The polymorphism was low (9.67%) and varied among and within markers. The SCoT markers exposed the highest average values of polymorphism information content (0.06), resolving power (0.91) and diversity index (0.08), while ISSR induced the highest expected heterozygosity (0.06) and marker index (0.08). The UPGMA dendrogram based on data from all the molecular markers separated the genotypes into three main clusters, with a partial geographic-based relationship among the genotypes. Out of the six accessions evaluated for callus induction in vitro, five were potent to induce callus, with a frequency ranging from 90.4 to 97.5% and no significant differences among the five accessions tested using ANOVA. Two medium combinations showed superior results: MS amended with 2,4-D (4.44 mg/L) + Kin (0.22 mg/L) and B5 with 2,4-D (8.88 mg/L) + Kin (0.22 mg/L). Statistically significant variations in the relative growth rate of the produced callus were detected among accessions, where EG-4 accessions induced the highest values, followed by EG-5. All medium combinations, including 2,4-D alone, exhibited significant superiority compared with those including both 2,4-D and Kin. Our findings exposed low variability among the studied cumin accessions, implying the real need for more effort to assess wider populations from different geographic regions together with the need for reliable diversification programs.
Mansour M. El-Fawy, Kamal A. M. Abo-Elyousr, Nashwa M. A. Sallam, Rafeek M. I. El-Sharkawy, and Yasser Eid Ibrahim
MDPI AG
Wood vinegar (WV) by-product of charcoal production is considered one of the most promising alternatives to synthetic pesticide and fertilizer applications, especially for organic production. Our goal in this study is to evaluate the efficacy of guava (Psidium guajava) WV to control Colletotrichum coccodes, which causes black dot disease, and how it influences potato plant development and yield. This study tested the efficacy of guava WV against the pathogen both in vitro and under greenhouse conditions. Different guava WV concentrations were tested on pathogen growth development, including 0, 0.25%, 0.50%, 1%, 2%, and 3% (v/v). Data revealed that the pathogen’s mycelial growth was significantly inhibited at all the concentrations, and the highest inhibition (100%) was obtained at 3% guava WV. In greenhouse trials conducted for two seasons (2021 and 2022), guava WV applied as a foliar spray at the concentration of 2% and 3% considerably reduced the potato black dot severity evaluated as stem colonization (average of 22.9% for 2021, average of 22.5% for 2022), root covering with sclerotia (average of 21.7% for 2021, average of 18.3% for 2022) and wilted plants percentage (average of 27.8% for 2021, average of 33.3% for 2022). Overall, guava WV also showed a positive effect on plant growth by increasing plant height, stem diameter, and tuber yield per plant of treated potato in both seasons. Gas chromatography-mass spectrometry (GC-MS) analyses revealed the presence in guava WV of phenols, esters, organic acids, antioxidants, and alcohols. In conclusion, guava WV could represent a viable alternative for potato black dot disease management and for plant growth promotion.
Sherif S. Hindi, Jamal S. M. Sabir, Uthman M. Dawoud, Iqbal M. Ismail, Khalid A. Asiry, Zohair M. Mirdad, Kamal A. Abo-Elyousr, Mohamed H. Shiboob, Mohamed A. Gabal, Mona Othman I. Albureikan,et al.
MDPI AG
Passivated-carbon quantum dots (P-CQDs) have been attracting great interest as an antimicrobial therapy tool due to their bright fluorescence, lack of toxicity, eco-friendly nature, simple synthetic schemes, and possession of photocatalytic functions comparable to those present in traditional nanometric semiconductors. Besides synthetic precursors, CQDs can be synthesized from a plethora of natural resources including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC). Converting MCC into NCC is performed chemically via the top-down route, while synthesizing CODs from NCC can be performed via the bottom-up route. Due to the good surface charge status with the NCC precursor, we focused in this review on synthesizing CQDs from nanocelluloses (MCC and NCC) since they could become a potential source for fabricating carbon quantum dots that are affected by pyrolysis temperature. There are several P-CQDs synthesized with a wide spectrum of featured properties, namely functionalized carbon quantum dots (F-CQDs) and passivated carbon quantum dots (P-CQDs). There are two different important P-CQDs, namely 2,2′-ethylenedioxy-bis-ethylamine (EDA-CQDs) and 3-ethoxypropylamine (EPA-CQDs), that have achieved desirable results in the antiviral therapy field. Since NoV is the most common dangerous cause of nonbacterial, acute gastroenteritis outbreaks worldwide, this review deals with NoV in detail. The surficial charge status (SCS) of the P-CQDs plays an important role in their interactions with NoVs. The EDA-CQDs were found to be more effective than EPA-CQDs in inhibiting the NoV binding. This difference may be attributed to their SCS as well as the virus surface. EDA-CQDs with surficial terminal amino (-NH2) groups are positively charged at physiological pH (-NH3+), whereas EPA-CQDs with surficial terminal methyl groups (-CH3) are not charged. Since the NoV particles are negatively charged, they are attracted to the positively charged EDA-CQDs, resulting in enhancing the P-CQDs concentration around the virus particles. The carbon nanotubes (CNTs) were found to be comparable to the P-CQDs in the non-specific binding with NoV capsid proteins, through complementary charges, π-π stacking, and/or hydrophobic interactions.
Bahaa E. S. Abd El-Fatah, Muhammad Imran, Kamal A.M Abo-Elyousr, and Amer F. Mahmoud
Springer Science and Business Media LLC
Abstract Background The bacterial speck disease of tomato caused by a bacterial pathogen Pseudomonas syringae pv. tomato is a most important disease causing severe crop losses. Methods and results Present study was conducted to investigate and characterize the population diversity of P. syringae pv. tomato pathogen isolated from infected tomato plants from various regions of Egypt. Significant variation among the isolates was observed which demonstrated considerable virulence. All isolates were pathogenic and the CFU population recovered from inoculate tomato leaves by isolate Pst-2 was higher than other isolates. Genetic disparity among the isolates was investigated by PCR analysis by amplifying hrpZ gene using random amplified polymorphic DNA (RAPD), sequence-related amplified polymorphism (SRAP), and inter-simple sequence repeats (ISSR) markers. The amplified products for ITS1 were found to have 810 bp length whereas 536 bp length was observed for hrpZ gene using primer pairs (1406-f/23S-r) and (MM5-F, MM5-R) respectively. The restriction analysis of amplified regions “ITS” and hrpZ by using 5 and 4 endonucleases respectively demonstrated slight variation among the bacterial isolates. The results of RAPD, ISSR and SRAP showed higher polymorphism (60.52%) within the isolates which may assist for successful characterization by unique and specific markers based on geographical distribution, origin and virulence intensity. Conclusion The results of present study suggested that the use of molecular approach may provide successful and valuable information to differentiate and classify P. syringae pv. tomato strains in future for the detection and confirmation of pathogenicity.
Mansour M. El-Fawy, Rafeek M. I. El-Sharkawy, Kamal A. M. Abo-Elyousr, and Moshref M. Sh. Ahmed
Springer Science and Business Media LLC
Mohamed H. Abd El-Wahed, Mamdouh A. Eissa, Najeeb M. Almasoudi, and Kamal A.M. Abo-Elyousr
Elsevier BV
Muhammad Imran, Kamal A. M. Abo-Elyousr, Mohammad S. AL-Harbi, Esmat F. Ali, Nashwa M. A. Sallam, and Hadeel M. M. Khalil Bagy
Springer Science and Business Media LLC
Muhammad Imran, Kamal A. M. Abo-Elyousr, Mohamed E. El-Sharnouby, Esmat F. Ali, Nashwa M. A. Sallam, Hadeel M. M. Khalil Bagy, and Ismail R. Abdel-Rahim
Springer Science and Business Media LLC
Nashwa M. A. Sallam, Heba-Alla S. AbdElfatah, Hadeel M. M. Khalil Bagy, Ameer Elfarash, Kamal A. M. Abo-Elyousr, Edward J. Sikora, and Ahmed Sallam
Frontiers Media SA
Controlling early blight of tomatoes using endophytic bacteria is an eco-friendly and sustainable approach to manage this common fungal disease caused by Alternaria solani, Alternaria alternata, and Curvularia lunata. Endophytic bacteria are microorganisms that live inside plant tissues without causing harm and can help protect the host plant from pathogens. In this work, twenty endophytic bacterial isolates from tomato healthy plants were tested against pathogenic fungal isolates that caused early blight disease in vitro. Out of the 20 tested isolates, three (B4, B7, and B17) were considered effective isolates against the growth of fungal pathogens. The three isolates were recognized as Enterobacter cloacae HS-6 (B4), Pseudomonas gessardii HS-5 (B 7), and Pseudomonas mediterranea HS-4 (B17) using 16s-rDNA sequencing. Different concentrations of bacterial cultural diltrates at 20, 40, and 60% were tested for their antagonistic effects on the development of pathogenic fungi in vitro. The lowest dry weights of pathogenic isolates in all bacterial culture filtrates were discovered at 60%. In all culture filtrates, phenolic compounds showed the largest peak area. Under greenhouse conditions, the least disease severity of tomato early blight was found for E. cloacae and its culture filtrate compared to other treatments. Real-time PCR was used to examine the expression pattern of the defense response gene β-1.3 glucanase gene in infected tomato plants with pathogenic fungi (control) as well as its relations with efficient biocontrol agent (E. cloacae). The expression of the gene increased substantially and significantly after three days from the inoculation-infected plants with C. lunata and E. cloacae while it reached the maximum after five days from the inoculation with A. alternata, A. solani and E. cloacae. Our study concluded that the endophytic bacterial isolate E. cloacae can be considered a promising biocontrol agent for preventing tomato early blight.
Suleiman K. Bello, Samir G. AL-Solaimani, and Kamal A. M. Abo-Elyousr
Informa UK Limited
Muhammad Imran, Kamal A. M. Abo-Elyousr, Magdi A. A. Mousa, and Maged M. Saad
Frontiers Media SA
IntroductionAlternaria solani is a challenging pathogen in the tomato crop globally. Chemical control is a rapid approach, but emerging fungicide resistance has become a severe threat. The present study investigates the use of culture filtrates (CFs) of three species of Trichoderma spp. to control this disease.MethodsHighly virulent A. solani strain and three Trichoderma fungal strains viz., T. harzianum (Accession No: MW590687), T. atroviride (Accession No: MW590689) and T. longibrachiatum (Accession No: MW590688) previously isolated by authors were used in this study. The efficacy of culture filtrates (CFs) to mitigate early blight disease were tested under greenhouse and field conditions, experiments were conducted in different seasons of 2020 using a tomato variety “doucen”.Results and discussionThe CFs of T. harzianum, T. longibrachiatum, and T. atroviride significantly inhibited the in vitro mycelial growth of A. solani (62.5%, 48.73%, and 57.82%, respectively, followed by control 100%). In the GC–MS analysis of Trichoderma CF volatile compounds viz., harzianic acid (61.86%) in T. harzianum, linoleic acid (70.02%) in T. atroviride, and hydroxymethylfurfural (68.08%) in the CFs of T. longibrachiatum, were abundantly present. Foliar application of CFs in the greenhouse considerably reduced the disease severity (%) in all treatments, viz., T. harzianum (18.03%), T. longibrachiatum (31.91%), and T. atroviride (23.33%), followed by infected control (86.91%), and positively affected the plant biomarkers. In the greenhouse, the plants treated with CFs demonstrated higher flavonoids after 6 days of inoculation, whereas phenolic compounds increased after 2 days. The CF-treated plants demonstrated higher antioxidant enzymes, i.e., phenylalanine ammonia-lyase (PAL) and peroxidase (POD), after 4 days, whereas polyphenol oxidase (PPO) was higher after 6 days of inoculation, followed by healthy and infected controls. In open field conditions, disease severity in CF-treated plants was reduced in both seasons as compared to naturally infected plants, whereas CF-treated plants exhibited a higher fruit yield than controls. The present results conclude that CFs can be a potential biocontrol candidate and a promising alternative to the early blight pathogen for sustainable production.
RECENT SCHOLAR PUBLICATIONS
MOST CITED SCHOLAR PUBLICATIONS
Publications
1- Bereika, M.F.F., M.H.A. Moharam, Kamal A.M. Abo-Elyousr and M.R. Asran 2020. Control of brown rot and wilt disease of potato caused by Ralstonia solanacearum using some plant water extracts. Journal of Sohag Agriscience (JSAS),
2- Bereika F.F. Mohamed, Nashwa M.A. Sallam, Saad A.M. Alamri, Kamal A.M. Abo-Elyousr Mohamed Hashem and Yasser S. Mostafa 2020. Approving the biocontrol strategy of potato wilt caused by Ralstonia solanacearum on field scale using Enterobacter cloacae PS14 and Trichoderma asperellum T34. Egyptian Journal of Biological Pest Control, 30: 61.
3- Yasser S. Mostafa, Saad A. Alamri, Mohamed Hashem, Nivien A. Nafady, Kamal A.M. Abo-Elyousr, Zakaria A. Mohamed 2020. Thermostable cellulase biosynthesis from Paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation. Open Life Sci. 15: 185–197
4- Adel Kamel Madbouly; Kamal A.M. Abo-Elyousr; Ismail Mohamed Ismail. 2020. Biocontrol of Monilinia fructigena the causal agent of brown rot of stored apple fruits using certain endophytic yeasts. Biological Control 144C: 104239 DOI:10.1016/j.
5- Bahaa E. S. Abd El-Fatah, Mohamed Hashem, Kamal A. M. Abo-Elyousr, Hadeel MMK Bagy and Saad A.M. Alamri 2020. Genetic and biochemical variations among sugar beet cultivars resistant to Cercospora leaf spot. Physiological and Molecular Plant Pathology 109C: 101455 DOI:10.1016/j.
6- Kamal A.M. Abo-Elyousr, Saad A.M. Alamri Mohamed M. A. Hussein, Mohamed A.H. Hassan and Bahaa E. S. Abd El-Fatah and Mohamed Hashem 2020. Molecular disparities among Botrytis species involving in onion umbel blight disease and its management using Bacillus subtilis PHYS7. Egyptian Journal of Biological Pest Control, 30: 1 DOI:10.1186/s41938-020-0205-X
7- Kamal A.M. Abo-Elyousr, Hadeel M. M. Khalil Bagy, Mohamed Hashem, Saad A.M. Alamri, and Yasser S. Mostafa 2019. Biological Control of Tomato Wilt Caused by Clavibacter michiganensis subsp. michiganensis Using Formulated Plant Growth-Promoting Bacteria. Egyptian Journal of Biological Pest Control, 29:54
8- Al-Sman K. Mohamed, Kamal A.M. Abo-Elyousr, Eraky Amal, El-Zawahry Aida 2019. Efficiency of Pseudomonas spp-based formulation for controlling root rot disease of black cumin under greenhouse and field conditions. Archive of Phytopathology and Plant Protection 52: (19-20): 1313-1325. DOI:10.1080/03235408.2019.1707384
9- Al-Sman K. Mohamed, Kamal A.M. Abo-Elyousr, Eraky Amal, El-Zawahry Aida 2019. Potential activities of Bacillus simplex as biocontrol agent against root rot of Nigella sativa caused by Fusarium camptoceras. Egyptian Journal of Biological Pest Control, 29:79
10- Hadeel M. M. Khalil Bagy and Kamal A.M. Abo-Elyousr, 2019. Antibacterial activity of some essential oils on bacterial spot disease of tomato plant caused by Xanthomonas axonopodis pv. vesicatoria. International Journal of Phytopathology 8: 53-61 DOI: 10.33687/
11- Nivien A. Nafady, Saad A.M. Alamri, Elhagag A. Hassan, Mohamed Hashem, Yasser S. Mostafa, Kamal A.M. Abo-Elyousr 2019. Application of ZnO-nanoparticles to manage Rhizopus soft rot of sweet potato and prolong shelf life. Folia Hort. 31(2), 319-329 DOI: 10.2478/fhort-2019-0025