Sri Nabawiyati Nurul Makiyah
@umy.ac.id
School of Medicine, Faculty of Medicine and Health Sciences
Universitas Muhammadiyah Yogyakarta
Scopus Publications
Scopus Publications
Sri Nabawiyati Nurul Makiyah and Sartika Puspita
Universitas Airlangga
Background: The trend of studies on dental medicaments is increasing rapidly. Antibacterial or anti-inflammatory activity is most frequently studied. Ovalbumin is one of the proteins whose benefits have been studied, but these benefits are still limited because of ovalbumin’s potential for proliferative bioactivity. Purpose: The aim of this study is to examine ovalbumin’s potential as a woundhealing medicament through molecular docking analysis on a protein related to the extracellular signal-regulated kinases/mitogenactivated protein kinase (ERK/MAPK) signaling pathway. Methods: Ovalbumin was hydrolyzed through BIOPEP-UWM (The BIOPEPUWM™ database of bioactive peptides). Protein target and interaction were predicted using Similarity Ensemble Approach target prediction webserver, SuperPred webserver, STRING webserver, and Cytoscape version 3.9.1. Selected fragments were docked using Autodock Vina in PyRx 0.8 with Tukey’s multiple comparison test and Biovia Discovery Studio version 19.1.0.18287 for visualization. Results: This study found that ovalbumin has the potential to positively regulate cell proliferation, angiogenesis, and fibroblast growth factor production. Six of the 131 fragments of ovalbumin could interact with 73 proteins, and the 20 proteins with the highest probability and score of betweenness centrality showed potential for bioactivity. Five fragments and povidone-iodine interacted inside the Adenosine triphosphate (ATP) phosphorylation site of ERK2, whereas fragment 1 (F1) and glycerin interacted outside the site. F1 could decrease the binding energy required for adenosine 5′-[,-methylene]triphosphate or an ATP-analogue chemical compound to interact with ERK2 compared to the control, with a score that was not significant. Conclusion: Ovalbumin has the potential to induce cell proliferation by affecting ERK2-ligand interactions.
Sri Nabawiyati Nurul Makiyah, Sri Tasminatun, Muhammad Sasmito Djati, Muhaimin Rifa’i, and Widodo Widodo
Universitas Gadjah Mada
Steroidal saponin compound in Dioscorea species is one of the crucial substance because it has some biological functions, such as immunomodulatory and anti-allergic agent. This study aims to analyze the effect of steroidal saponin of Dioscorea alata L. on CD4 T cells activation, cytokines IL-4, IL-5 and TGF-β. This study used primary lymphocyte cell which isolated from BALB/c mice spleen. Mice lymphocyte were cultured in the complete medium with presence of various concentrations (50, 25 and 12.5 µg/mL) of three isolate of steroidal saponin of D. alata for 24 h. Diosgenin was used as positive control. After 24 h of treatment, the activation of lymphocyte, IL-4 and IL-5 production was determined by Flowcytometry analysis. Data were analyzed using student t-test with p < 0.05 and p < 0.01. The results showed that the low concentration of Isolate 1 (12.5 µg/mL) was found to be effective to stimulate the activation of CD4 T cells, reduce the production of IL4 and IL-5 by CD4 T cells and increase TGF-β production. These results showed that a low concentration of steroidal saponin of D. alata could be considered as immunomodulatory and anti-allergic agent. Purification and identification of each isolate isolated from DAE is in progress.
Erna Rochmawati, Endrat Kartiko Utomo, and Sri Nabawati Nurul Makiyah
Wiley
The study aimed to determine the effects of giving range of motion (ROM) twice a week as a form intradialytic exercise on patients' dialysis adequacy and quality of life. A total of 48 eligible participants were recruited and completed this study. The intervention group (n=24) received twice a week range of motion exercise over a 4 weeks period. The outcomes were assessed at pre-test and at the end of the intervention. The findings indicated that ROM was effective in improving dialysis adequacy, in terms of ureum retention ratio (p = 0.027), and Kt/V (p=0.017). Quality of life improved significantly particularly in the domains of symptom and effect of kidney disease. Integrating ROM into dialysis care shows as a potential intervention to improve dialysis adequacy and quality of life for patients undergoing haemodialysis. Future research should evaluate its efficacy using a randomised clinical trial design and larger sample of patients.
Sri Nabawiyati Nurul Makiyah, Masaki Kita, Ika Setyawati, and Sri Tasminatun
Secretariat of The Indonesian Biomedical Journal
BACKGROUND: Dioscorea alata L. tubers (DA) are suspected to prevent diabetes mellitus (DM) because they have a low glycemic index. However, only a few reports about the anti-diabetic effect of DA were reported up to date. This study aims to analyze the effect of DA consumption on several diabetic biomarkers through in vitro, in vivo, and in silico analysis.METHODS: In vitro experiments were conducted by observing the anti-inflammatory activity of DA extract, steroidal saponins (SDA) isolated from DA, and diosgenin in lymphocyte cell cultures. The tumor necrosis factor (TNF)-α and interferon (IFN)-γ percentages were analyzed by flow cytometry. In vivo study involved 24 healthy adolescents that were given a boiled DA 10 hours post-prandial. The blood sugar levels were measured at 0, 30, 60, and 120 min after treatment. Furthermore, the in silico study was carried out by analyzing the active compounds and predicting the biological activity, the target proteins, and interactions of target proteins with compounds contained in DA .RESULTS: DA extract, SDA isolated from DA, and diosgenin at 50 µg/mL significantly reduced pro-inflammatory cytokines TNF-α and IFN-γ in lymphocyte cell culture. The blood glucose levels in the DA group were lower at 30 and 60 min after treatment. Based on the in silico study, the anti-diabetic activity of DA was speculated to be attributed to the mechanisms of anti-hyperglycemia, prevention of mitochondrial dysfunction, anti-inflammation, and treated insulin resistance. Several proteins included in the DM pathway became the protein target of compounds contained in DA.CONCLUSION: DA potentially have an anti-diabetic activity through several mechanisms.KEYWORDS: hyperglycemia, inflammation, insulin resistance, yam
L N Aini, J Prasetyo, S N Nurul Makiyah, and Mulyono
IOP Publishing
Cangkringan Subdistrict is one of areas in Southern flank of Mount Merapi, Special Region of Yogyakarta which is prone to eruptions. Landuse changes due to eruptions often occur in this area. As a result, the Cangkringan area is prone to food security, due to the damage and landuse changes that have occurred. Yam is a tuber crop that is resistant to nutrient-poor land and has not been widely cultivated. The prospect of developing Yam is very large as a way of diversifying food. Therefore, an effort is needed to evaluate the land for cultivation of Yam on marginally lands affected by the eruption by determining the characteristics of the land as the basis for determining land suitability. The research was conducted using analytical descriptive method and analyzed using the matching method between land quality and land characteristics as parameters with land suitability class criteria. The results showed that the actual land suitability class in Cangkringan sub-district for Yam cultivation was S3nr, na, eh, tc with limiting factors for nutrient retention, total N content, land slope, and temperature. While the potential land suitability is S3eh, tc with limiting factors for land slope and temperature. Improvements that can be made include the addition of organic matter, fertilization, making terraces and planting on land that is not shaded.
Linda Yulyani, Sri Nabawiyati Nurul Makiyah, and Sulistyaningsih Sulistyaningsih
DiscoverSys, Inc.
Exclusive breastfeeding behavior of
Sri Nabawiyati Nurul Makiyah, Sri Tasminatun, Puguh Novi Arsito, Khairina Nurul Fauziah, Dieny Rizka Nugrahanti, and Annisa Putriani
DiscoverSys, Inc.
Subchronic toxicity of piperine in piper nigrum on the histology of the kidney, liver, and lungs of mice (Mus musculus L.) Sri Nabawiyati Nurul Makiyah1*, Sri Tasminatun2, Puguh Novi Arsito2, Khairina Nurul Fauziah3, Dieny Rizka Nugrahanti3, Annisa Putriani3 Introduction: Piperine is a compound that has many health benefits, but piperin has toxic effects that endanger the human body. The purpose of this study was to determine the toxic dose of subchronic piperine administration using histology of the liver, kidneys, and lungs of mice (Mus musculus L). Method: This is a quasi-experimental research with posttest only control group design. Thirty male BALB/c mice were divided into five groups. Piperine was administered orally to the mice. The normal control group and four treatment groups at dosage of 17.5, 35, 70, and 140 mg/kg body weight for 21 days. At the day-22, the liver, kidneys, and lungs were removed and prepared using the HE staining technique. Scores of liver, kidney, and lung damage are recorded. The data was analyzed using one-way ANOVA with the post hoc Tukey test. Result: The liver histological damage score after administration of piperine doses of 35, 70, and 140 mg/kg significantly different from the control group. The kidney and lung damage scores after 140 mg/kg administration showed significant differences from the control group. The conclusion is piperine at dosage of 35, 70, and 140 mg/kg was toxic to the liver, and piperine at doses of 140 mg/kg was toxic to the kidney and lung histology of BALB/c mice. Conclusion: Piperine should not be used at doses of 35 mg/kg body weight or larger.
Gracea Petricka, SN Nurul Makiyah, and Retno Mawarti
Belitung Nursing Journal
Background: It is estimated that 49.2% of Indonesian adolescents (10-19 years) have iron deficiency of anemia. Green vegetables can be used as an alternative consumption to meet the body's need for iron. Kelakai (Stenochlaena palustris) containing 291.32 mg-100 g of Fe has been consumed by Dayak ethnic society to prevent anemia.Objective: The aim of this study was to determine the effect of Kelakai (Stenochlaena palustris) consumptions to increase hemoglobin levels among late adolescents (17-19 years).Methods: It was a quasy-experiment with pre-test and post-test study on anemic Midwifery students (8-11 g / dl) of Betang Asi Midwifery Academy of Palangka Raya in September 2017. In which Kelakai was given as dietary supplements of the subject line and ferrous fumarate tablet as a control. There were 66 participants recruited by purposive sampling, divided into 33 subjects for each group. Kelakai (Stenochlaena palustris) (250 mg) and ferrous fumarate tablet (60 mg) were administrated daily for a week. Hemoglobin levels were measured before and after intervention using the hemoglobin testing system quick-check tool. Data were analyzed using paired and independent t-test.Result: The result showed of significant increases on hemoglobin levels (3.24 g / dl) after consuming Kelakai (Stenochlaena palustris) for a week (p≤0.05). The hemoglobin levels after intervention in the control group were 0.03 g / dl higher than Kelakai group, but based on the analysis, there was no significant difference on both groups (p≥0.05).Conclusion: Kelakai is proven to increase hemoglobin levels. Thus, Kelakai (Stenochlaena palustris) is recommended a food supplementation to prevent iron deficiency of anemia.
Sri Nabawiyati Nurul Makiyah, Widodo, Muhaimin Rifa’i, and Muhamad Sasmito Djati
Author(s)
Naive T cells and activated T cells express different adhesion molecules and patterns of migration. Naive T cells express CD62L. The research aims to assess the CD4+CD62L+ and CD8+CD62L+ profile of BALB/c mice model allergy on the sensitivity, the challenge and the gastrointestinal tract allergy phase after treatment with ethanol extract of Dioscorea alata L. (EEDA). An experimental study with post test only control group design using 63 BALB/c mice was divided into seven groups: control group (I) and treatment groups with the ethanol extract of D. alata L. tubers dose (0.00, 0.17, 2.01, 10.04) g · kg−1 bw (II-V); an antihistamine drug treatment (VI) and diosgenin treatment of 200 mg · kg−1 bw (VII). Treatment groups induced with ovalbumin i.p. on 15 d and 22 d, orally on 23 d to 30 d subsequently. Mice were sacrificed on (18, 25 and 31) d, the spleen was taken and isolated to measure CD4+CD62L+ and CD8+CD62L+ profile with flowcytometry. The results showed that EEDA dose (0.17, 10.04) g · kg−1 and drug on...