@tnau.ac.in
Professor, Soil Science and Agricultural Chemistry
Tamil Nadu Agricultural University
Soil fertility, Heavy metal bio-remediation, Organic amelioration for salt affected soils
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
R. Renuka, K. Renuka Devi, M. Sivakami, and T. Thilagavathi
Springer Science and Business Media LLC
The silver nanoparticles (AgNPs) with biogenic synthesis employing plants have been grown to be a promising alternative to the traditional chemical synthesis method. The AgNPs were triumphantly synthesized using Solanum torvum (Turkey berry) fruit extract characterized by UV–Visible spectrum as a consequence of strong surface plasmon resonance peak at 442 nm. The existence of bio-molecules acting as a capping and stabilizing agent in the AgNPs with Solanum torvum was revealed by Fourier transform infrared spectroscopy analysis. X-ray diffraction investigation for the greener route synthesized AgNPs witnessed as face centered cubic crystalline nature along with an average crystalline size of 25 nm. Scanning electron microscope images evidenced that the nanoparticles have an irregular shape of hexagonal morphology and the presence of silver element was depicted in Energy-dispersive X-ray spectroscope profile. The AgNPs were explored against two gram-positive and negative bacteria via antibacterial activity. The results showed that biosynthesized AgNPs have a strong inhibitory effect on bacteria with a more pronounced inhibition against Bacillus subtilis. AgNPs with very high antibacterial activity can also be employed as an efficient material for different medicinal applications.
Sivakami M., Renuka Devi K., Renuka R., and Thilagavathi T.
Elsevier BV
Abstract The green synthesis of magnetic Fe nanoparticles (NPs) was prepared via Cinnamomum Verum bark extract. The UV–vis analysis explained the formation of Fe NPs with SPR band at 288 nm. The powder X-ray diffraction analysis (XRD) explicated the mean crystallite size was at 36 nm. The Fourier Transform Infra-Red analysis (FTIR) revealed the functional groups in the prepared Fe NPs. The Scanning Electron Microscope (SEM) and High-Resolution Transmission Electron Microscope (HR-TEM) analyses notified the circular and spherical shaped Fe NPs with the size at 20−50 nm. The Energy dispersive X-ray spectroscopy (EDS) and mapping analyses validated the formation of Fe NPs with purity. The Vibrating Sample Magnetometer (VSM) revealed the paramagnetic behavior of prepared Fe NPs. The phytochemical analysis described the phytochemicals compounds which present in Cinnamomum Verum bark extract. The Antibacterial assay described the much higher inhibition zone of prepared Fe NPs upon human pathogenic bacteria. The Antioxidant (DPPH) assay described the efficient scavenging behavior of Fe NPs with 89 % at 80 μg/mL concentration. The Anti-inflammatory assay explicated the potential protein denaturation behavior of Fe NPs with 87 % at 500 μg/mL concentration. The Anti-diabetic assay reported the much higher potential efficiency of prepared Fe NPs with 84 % at 500 μg/mL concentration.
R. Renuka, K. Renuka Devi, M. Sivakami, T. Thilagavathi, R. Uthrakumar, and K. Kaviyarasu
Elsevier BV
Abstract Biological synthesis of metal nanoparticles is one of the effortless, cost - effective, environmental - friendly methods and scale down the handling of toxic chemicals. The silver nanoparticles were triumphantly synthesized from the silver nitrate solution through a greener route using Indian gooseberry (phyllanthus emblica) fruit extract and the prepared particles are of well-balanced shape and size. The stabilization and the bio-reduction of silver nanoparticles were described by adopting UV–vis spectrometry interprets the surface plasmon resonance peak at 449 nm. The functional groups of phyllanthus emblica fruit extract and the silver nanoparticles were diagnosed by using Fourier transform Infrared spectrum (FTIR). The crystalline nature of face-centered cubic (FCC) was examined by X-ray diffraction (XRD) and the crystalline size ranges between 19 nm to 45 nm with an average size 30 nm. Scanning electron microscope (SEM) witnessed the hexagonal shape of silver nanoparticles and Energy-dispersive X-ray spectroscope (EDX) assured the well-established fabrication of nanocrystalline silver element. The synthesized nanoparticle exhibits significant antibacterial effects and very clear zone of inhibition against Klebsiella pneumoniae and Staphylococcus aureus bacterias.
Govindarajan Kathiravan and Thilagavathi T
Innovare Academic Sciences Pvt Ltd
<p>ABSTRACT<br />Objective: The potential compound of taxol has been used treatment of cancer in the medical field. This study is focused on the extraction of taxol<br />from Fusicoccum sp.<br />Methods: The pathogenic strain was grown in MID medium for the taxol production and extracted from the Fusicoccum culture filtrate and processed<br />to the ultraviolet (UV)-spectroscopy analysis as well as infrared (IR) spectra analysis was done, and the sample was performed to the high-performance<br />column chromatography and further processed to the random amplified polymorphic DNA (RAPD) analysis of specific primer is PGFO1, PGFO2 are<br />performed.<br />Results: UV-visible spectral analysis showed maximum value for 273 nm. The result of IR-spectrum analysis to find out the functional groups present<br />in sample and characteristic peak to conform the existences of OH group. The presence of taxol was confirmed using column chromatography.<br />An analysis of taxol production was on the external standard of authentic taxol. The column chromatography process shows the amount of taxol<br />production by Fusicoccum spp. 325 µg/L. RAPD analysis indicates the genetic relation among all isolates of fungus was analyzed by two random<br />primers PGF01 and PGF02.<br />Conclusion: The present investigation revealed that the taxol production from biological source was a significant effect, and the presence of taxol<br />amount is analysis in Fusicoccum sp. 325 µg/L. The RAPD analysis of genetic stability of in vitro grown fungus and genotypic with two different<br />primers shows that five fungus species with corresponding primer efficiency was amplified.<br />Keywords: Taxol production, Fusicoccum sp., Ultraviolet-spectroscopy, Infrared, Spectrum analysis, Scanning electron microscopy.</p>