Bruno de Carvalho Dornelas
@ufu.br
Pathology Department
Federal University of Uberlandia
RESEARCH, TEACHING, or OTHER INTERESTS
Medicine, Pathology and Forensic Medicine
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Lídia Laura Salvador Ramos, Iago Resende Carvalho, Diogo Fernandes dos Santos, Andrea de Martino Luppi, and Bruno de Carvalho Dornelas
Georg Thieme Verlag KG
A 60-year-old man presented with vertigo, paraparesis, and dysarthriathat evolved over 3 months. A magnetic resonance imaging (MRI) scan revealed nodular masses in the pons, peduncles, and cerebellar hemispheres, indicating a metastatic neoplasm ( ► Figure 1 ). A craniectomy was performed, showing dense histiocyte proliferation, Touton giant cells, and lymphocytic in fi ltrate consistent with Erdheim-Chester disease ( ► Figure 2 ). The cytogenetic analysis con fi rmed a BRAF V600 mutation. Vemurafenib therapy was initiated. Six months later, the patient still exhibited dyslalia and left hemiparesis, and a follow-up MRI scan was performed, revealing an area of sequelae ( ► Figure 3 ). Erdheim-Chester disease is a rare non-Langerhans histiocytosis, with neurological symptoms occurring in 41% of the cases. 1,2
Diogo Fernandes dos Santos, Leonardo Peixoto Garcia, Iago Resende Carvalho, Bruno de Carvalho Dornelas, and Isabela Maria Bernardes Goulart
Lepra
Bruno de Carvalho Dornelas, Willian Vargas Tenório da Costa, João Pablo Ferraz de Abreu, Juliana Salomão Daud, Felipe dos Anjos Rodrigues Campos, Deiriene Rodrigues de Oliveira Campos, Douglas Eulálio Antunes, Lúcio Borges de Araújo, Diogo Fernandes dos Santos, Cleverson Teixeira Soares,et al.
Wiley
This study aimed to identify laboratory factors predicting leprosy relapse (LR) after multi‐drug therapy (MDT). A case–control study included 80 patients treated with MDT at a national reference center over 12 years. The Relapse Group had 40 patients who relapsed after an average of 89.2 months post‐MDT, while the Control Group had 40 patients who remained asymptomatic for an average of 113.1 months. Significant predictors of LR included neural/perineural lymphocytic infiltrate (OR = 4.67; p = 0.0076) and foamy granulomas (OR = 15.55; p = 0.0005), increasing odds by 4.7 and 15.6 times, respectively. The Relapse Group had a mean histological bacillary index (hBI) of 3.23+ compared to 1.8 in the Control Group (p = 0.004). An hBI ≥3+ had 72% sensitivity and 65% specificity for detecting LR (AUC = 0.72; p = 0.0002). Elevated anti‐phenolic glycolipid I (anti‐PGL‐I) IgM antibody levels (ELISA index, EI ≥1) were also associated with LR (OR = 4.67; p = 0.0031). An EI ≥3.6 had 71% sensitivity and 62% specificity (AUC = 0.70; p = 0.0012). Multivariate analysis indicated that neural/perineural infiltrate, foamy granulomas, hBI ≥ 1+, and EI ≥ 1 significantly predicted LR, with up to 94.32% probability. Conclusively, these factors can identify individuals at high probability of LR after MDT.
Bruno de Carvalho Dornelas, Willian Vargas Tenório da Costa, João Pablo Ferraz de Abreu, Juliana Salomão Daud, Felipe dos Anjos Rodrigues Campos, Deiriene Rodrigues de Oliveira Campos, Douglas Eulálio Antunes, Lúcio Borges de Araújo, Diogo Fernandes dos Santos, Cleverson Teixeira Soares,et al.
Springer Science and Business Media LLC
Stefano Machado, Egon Ewaldo Lindorfer Neto, Bruno de Carvalho Dornelas, Andrea de Martino Luppi, Elder Henrique de Oliveira, Paulo Cesar Marinho Dias, Maykell Queiroz dos Reis, and Diogo Fernandes dos Santos
Ovid Technologies (Wolters Kluwer Health)
An 80-year-old man from the Brazilian Midwest presented with tetraparesis with brachial predominance and hyperreflexia with 1 year of evolution. In investigation, cervical MRI showed intramedullary cystic formation (4.6 × 1.7 × 1.3 cm), and brain MRI was suggestive of neurocysticercosis (Figure 1). CT of the chest, abdomen, and pelvis and endoscopy and colonoscopy were normal. CSF showed hyperproteinorachia (113 mg/dL). The excision of the lesion of the spinal cord was uneventful, and the anatomopathological examination was compatible with neurocysticercosis (Figure 2). Slight improvement in paresis was noted on reassessment after 2 weeks, when albendazole (15 mg/kg/d) and corticosteroids were started for 4 weeks. This rare presentation of neurocysticercosis (Taenia solium) corresponds to 1%–3% of cases.1 Infectious etiologies should be considered in the differential diagnosis of spinal cord syndromes, mainly in emerging countries, where Dr. there is precarious basic sanitation and contact with contaminated water and food.2
Marcela Araujo de Oliveira Santana, Willian Vargas Tenório da Costa, Isadora Costa Celestino, Diogo Fernandes dos Santos, Bruno de Carvalho Dornelas, Matthew Martin Pavelka, Andrea De Martino Luppi, and Isabela Maria Bernardes Goulart
Frontiers Media SA
Mycobacterium leprae, the etiologic agent of leprosy, is an acid-fast-staining and slow-growing bacilli that infect macrophages and Schwann cells individually or through forming globi. The clinical presentation of leprosy is broad and depends on the host immune response. We report a case of a 42-year-old Brazilian man presenting with fever of unknown origin (FUO), anemia, wasting syndrome, and neuropathy. The diagnosis of lepromatous leprosy was made after an extensive investigation revealed the presence of M. leprae in the bone marrow. Bone marrow involvement in leprosy is rare and some authors believe the presence of M. leprae in the bone marrow can act as a reservoir of the disease facilitating future relapses. It is important to investigate bone marrow involvement in leprosy, especially when the patient presents with cytopenias and positive epidemiologic history.
Ana Cláudia Mendes do Nascimento, Diogo Fernandes dos Santos, Douglas Eulálio Antunes, Maria Aparecida Gonçalves, Marcela Araujo de Oliveira Santana, Bruno de Carvalho Dornelas, Luiz Ricardo Goulart, and Isabela Maria Bernardes Goulart
Elsevier BV
Isabela Maria Bernardes Goulart, Marcela Araujo de Oliveira Santana, Willian Vargas Tenório da Costa, Matthew Martin Pavelka, and Bruno de Carvalho Dornelas
Elsevier BV
Caddie Laberiano-Fernández, Joan Moreno Luján, Bruno de Carvalho Dornelas, Magali Franco Benites, Patricia Gutiérrez Quispe, Valeria Aguilar Vásquez, Andric Guerrero Espinoza, Elsa Guerra Guerra, Gabriela Gil-Arroyo Álvarez, Juan Astigueta-Pérez,et al.
Ecancer Global Foundation
The seventh session of the Oncological Pathology Conference (JoPaO) entitled ‘Pathological Anatomy in the context of the National Cancer Law: An overview of the Latin American experience’, was held virtually on July 15, 22 and 23. Peru was the headquarters for this event, where 17 national and international professors of high academic standing participated. They interacted in a multidisciplinary context through talks with national panellists and the general public. The recent promulgation of the ‘National Cancer Law’ fosters the development of discussion forums to analyse the national realities and uphold continuous learning about experiences in other Latin American countries with successful cancer programmes, in which pathology holds a principal role. The topics addressed during this JoPaO included the exchange of Latin American cancer management experiences, an emphasis on investments in and the development of strategic plans to improve care, the use of new technologies, laboratory quality control, and the need to advance scientific research.
Diogo Fernandes dos Santos, Douglas Eulálio Antunes, Bruno Carvalho Dornelas, Bruno Araujo da Cunha, Thales Junqueira Oliveira, Raquel Campos Pereira, Luiz Ricardo Goulart, and Isabela Maria Bernardes Goulart
Oxford University Press (OUP)
Abstract Background The early recognition of neural impairment in leprosy, especially in primary neural forms, represents a challenge in clinical practice and a peripheral nerve biopsy may be required for diagnostic confirmation. This study aims to characterize the epidemiological, clinical, electroneuromyographic, laboratory and histopathological aspects of patients undergoing peripheral nerve biopsy during investigation of primary neural cases in leprosy. Methods A total of 104 patients with peripheral neuropathy who were referred to a national reference centre for leprosy were biopsied from 2014 to 2018. All cases underwent clinical, laboratory, histopathological and electroneuromyographic evaluations. Results Of 104 biopsied patients, leprosy was confirmed in 89.4% (93/104). The biopsied nerves were the ulnar (67.8% [63/93]), superficial fibular (21.5% [20/93]), sural (8.6% [8/93]), radial (1.1% [1/93]) and deep fibular (1.1% [1/93]). Twenty-nine percent (27/93) presented histopathological abnormalities and 4.4% (4/93) presented acid-fast bacilli. Nerve and superjacent skin quantitative polymerase chain reaction were positive in 49.5% (46/93) and 24.8% (23/93) of cases, respectively. Patients with multiple mononeuropathy had a higher frequency of histopathological abnormalities (p=0.0077). Conclusions This study reinforces peripheral nerve biopsy's role as an important tool in the investigation of primary neural cases, contributing to the early diagnosis and also reducing diagnostic errors and the need for empirical treatment.
Sara Teixeira Soares Mota, Lara Vecchi, Douglas Alexsander Alves, Antonielle Oliveira Cordeiro, Gabriela Silva Guimarães, Esther Campos-Fernández, Yara Cristina Paiva Maia, Bruno de Carvalho Dornelas, Stephania Martins Bezerra, Victor Piana de Andrade,et al.
Elsevier BV
Fabíola Prado de Morais, Noah Romero Nakajima, Olívia Félix Marconi Andalécio, Pedro de Santana Prudente, Guilherme Emílio Ferreira, Andrea de Martino Luppi, Fernando Costa Mundim, Olga Maria Lima Aguiar, Juliana Salomão Daud Melo, and Bruno de Carvalho Dornelas
Hindawi Limited
Lipomas are rare primary heart tumors and may involve the endocardium, myocardium, or pericardium. Signs and symptoms depend on the tumor location and size. The intrapericardial lipoma we report has massive dimensions and mimics a pericardial effusion. A 38-year-old male complained of dyspnea and precordial pain. On physical examination, heart sounds were diminished. The patient had received extensive medication for a clinically suspected pericardial effusion due to heart failure. A voluminous mass resembling fat within the pericardial sac was revealed by transesophageal echocardiography and a computed tomography scan. The tumor was removed successfully by a subxiphoid surgical approach. The diagnosis of a 635 gram intrapericardial lipoma was confirmed by pathological examination. After surgery, the patient recovered well and was completely asymptomatic at a follow-up at 90 days. No medications were being taken since. The diagnosis of a pericardial effusion should be secured by imaging exams to avoid unnecessary medications. Cardiac lipomas can be readily recognized by their typical features on radiologic imaging. The surgical pathology examination confirms the diagnosis and rules out malignancy criteria.
Sara T. S. Mota, Lara Vecchi, Mariana A. P. Zóia, Fabrícia M. Oliveira, Douglas A. Alves, Bruno C. Dornelas, Stephania M. Bezerra, Victor P. Andrade, Yara C. P. Maia, Adriana F. Neves,et al.
MDPI AG
The human protein Polybromo-1 (PBMR1/BAF180) is a component of the SWI/SNF chromatin-remodeling complex that has been reported to be deregulated in tumors. However, its role in prostate cancer (PCa) is largely unknown. In this study, we described the PBRM1 transcriptional levels and the protein expression/localization in tissues of PCa patients and in prostatic cell lines. Increased PBRM1 mRNA levels were found in PCa samples, when compared to benign disease, and were correlated with higher Gleason score. We also verified that only the nuclear localization of PBRM1 protein is correlated with a more aggressive disease and high Prostate-Specific Antigen (PSA) levels in tissue microarrays. Intriguing expression patterns of mRNA and protein were identified in the cell lines. Although PBRM1 protein was restricted to the nuclei, in tumor cell lines in non-neoplastic cells, it was also present in vesicular-like structures that were dispersed within the cytoplasm. We knocked-down PBRM1 in the castration-resistant PCa (CRPC) cell line PC-3 and we verified that PBRM1 promotes the expression of several markers of aggressiveness, including EpCAM, TGF-β, and N-Cadherin. Therefore, our data supported the hypothesis that PBRM1 displays a pivotal role in the promotion and maintenance of the malignant behavior of PCa, especially in CRPC.
Bruno Tumang Frare, Yann Keller Silva Resende, Bruno de Carvalho Dornelas, Miguel Tannus Jorge, Valter Augusto Souza Ricarte, Lívia Maria Alves, and Luiz Fernando Moreira Izidoro
Hindawi Limited
Snakebite envenoming is a neglected public health issue in many tropical and subtropical countries. To diagnosis and treat snakebites may be challenging to health care personnel since sufficient information has not been yet provided. This review presents the clinical, therapeutic, and laboratory aspects of Crotalus durissus (South American rattlesnakes) victims. The clinical setting may show local effects such as little or no pain, mild edema, and recurrent erythema. In contrast, the systemic effects may be quite remarkable, such as changes due to neurological damage, intense rhabdomyolysis, incoagulability of the blood, and variations in the peripheral blood elements. The main complication is acute kidney injury. The appropriate treatment depends mainly on the correct recognition of the aggressor snake and the symptoms expressed by the victim. Rattlesnake venom can cause irreparable damage and lead to death. Therefore, a prompt diagnosis allows the immediate onset of proper serotherapy.