Jay Prakash Pandey

Verified email at bitmesra.ac.in

Professor Dept. of chemistry
Birla institute of Technology, Mesra, India




Graft copolymer
Microwave assisted polymerization


Scopus Publications

Scopus Publications

  • Microwave assisted synthesis of guar gum based biopolymeric macromolecule optimized as a flocculant for mineral ore processing
    Smita Singh, J.P. Pandey, and Gautam Sen

    International Journal of Biological Macromolecules, ISSN: 01418130, eISSN: 18790003, Volume: 220, Pages: 307-315, Published: 1 November 2022 Elsevier BV

  • Microwave-assisted cationization of Gum ghatti by grafting with diallyldimethylammonium chloride (DADMAC) and its applications as nano scavenger
    Anurag Mehta, Gautam Sen, and Jay Prakash Pandey

    Industrial Crops and Products, ISSN: 09266690, Volume: 179, Published: May 2022 Elsevier BV

  • Synthesis of a Novel Water-Soluble Graft Copolymer for Mineral Ore Beneficiation and for River Water Treatment towards Drinking Water Augmentation
    Anurag Mehta, Ayush Aryan, Jay Prakash Pandey, and Gautam Sen

    ChemistrySelect, eISSN: 23656549, Published: 17 January 2022 Wiley

  • Study on cocoonase, sericin, and degumming of silk cocoon: computational and experimental
    Preeti Anand, Jay Prakash Pandey, and Dev Mani Pandey

    Journal of Genetic Engineering and Biotechnology, ISSN: 1687157X, eISSN: 20905920, Published: December 2021 Springer Science and Business Media LLC
    Abstract Background Cocoonase is a proteolytic enzyme that helps in dissolving the silk cocoon shell and exit of silk moth. Chemicals like anhydrous Na2CO3, Marseille soap, soda, ethylene diamine and tartaric acid-based degumming of silk cocoon shell have been in practice. During this process, solubility of sericin protein increased resulting in the release of sericin from the fibroin protein of the silk. However, this process diminishes natural color and softness of the silk. Cocoonase enzyme digests the sericin protein of silk at the anterior portion of the cocoon without disturbing the silk fibroin. However, no thorough characterization of cocoonase and sericin protein as well as imaging analysis of chemical- and enzyme-treated silk sheets has been carried out so far. Therefore, present study aimed for detailed characterization of cocoonase and sericin proteins, phylogenetic analysis, secondary and tertiary structure prediction, and computational validation as well as their interaction with other proteins. Further, identification of tasar silkworm (Antheraea mylitta) pupa stage for cocoonase collection, its purification and effect on silk sheet degumming, scanning electron microscope (SEM)-based comparison of chemical- and enzyme-treated cocoon sheets, and its optical coherence tomography (OCT)-based imaging analysis have been investigated. Various computational tools like Molecular Evolutionary Genetics Analysis (MEGA) X and Figtree, Iterative Threading Assembly Refinement (I-TASSER), self-optimized predicted method with alignment (SOPMA), PROCHECK, University of California, San Francisco (UCSF) Chimera, and Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) were used for characterization of cocoonase and sericin proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), protein purification using Sephadex G 25-column, degumming of cocoon sheet using cocoonase enzyme and chemical Na2CO3, and SEM and OCT analysis of degummed cocoon sheet were performed. Results Predicted normalized B-factors of cocoonase and sericin with respect to α and β regions showed that these regions are structurally more stable in cocoonase while less stable in sericin. Conserved domain analysis revealed that B. mori cocoonase contains a trypsin-like serine protease with active site range 45 to 180 query sequences while substrate binding site from 175 to 200 query sequences. SDS-PAGE analysis of cocoonase indicated its molecular weight of 25–26 kDa. Na2CO3 treatment showed more degumming effect (i.e., cocoon sheet weight loss) as compared to degumming with cocoonase. However, cocoonase-treated silk cocoon sheet holds the natural color of tasar silk, smoothness, and luster compared with the cocoon sheet treated with Na2CO3. SEM-based analysis showed the noticeable variation on the surface of silk fiber treated with cocoonase and Na2CO3. OCT analysis also exemplified the variations in the cross-sectional view of the cocoonase and Na2CO3-treated silk sheets. Conclusions Present study enlightens on the detailed characteristics of cocoonase and sericin proteins, comparative degumming activity, and image analysis of cocoonase enzyme and Na2CO3 chemical-treated silk sheets. Obtained findings illustrated about use of cocoonase enzyme in the degumming of silk cocoon at larger scale that will be a boon to the silk industry.

  • Biochemical characterization of sericin isolated from cocoons of tropical tasar silkworm Antheraea mylitta raised on three different host plants for its prospective utilization
    K. Jena, Ruchi Kumari, J.P. Pandey, P.K. Kar, J. Akhtar, A.K. Singh, V.P. Gupta, and A.K. Sinha

    Journal of Asia-Pacific Entomology, ISSN: 12268615, Pages: 903-911, Published: August 2021 Elsevier BV

  • Protein–protein docking and molecular dynamics studies of sericin and cocoonase of silkworm: an insight for cocoon softening
    Nishi Prakash Tiwari, Jay Prakash Pandey, and Dev Mani Pandey

    Journal of Biomolecular Structure and Dynamics, ISSN: 07391102, eISSN: 15380254, Published: 2021 Informa UK Limited
    Cocoonase is known to digest the sericin protein that encapsulates the silkworm cocoon's fibroin protein. Silk fibroin and sericin are two types of proteins that make up silk, and accounts for around 20-30% of the overall cocoon weight. The aim of the study was to see the protein-protein interaction (PPI) and molecular dynamic study of sericin, cocoonase and protein-protein docked complex of silkworm by computational approaches. Here motif analysis, phylogenetic analysis, principal component analysis, root-mean-square deviation (RMSD), root mean square fluctuation, radius of gyration, structural and functional study of cocoonase and sericin as well as molecular docking study were carried out. The 33 amino acid residues of cocoonase shows interaction with 38 aa residues of sericin involving 4 disulphide bonds, 22 hydrogen bonds and 319 non-bonded contacts. The confirmational stability and flexibility of both the proteins as well as protein-protein complex were achieved at 70 ns of MD simulation study. RMSD-based data indicated that cocoonase is more stable than sericin and complex, and complex has a greater fluctuation with more compact (higher Rg) value than cocoonase and sericin, inferring higher conformational stability and flexibility of protein-protein complex than cocoonase and sericin. This study provides a new dimension for PPI study by computational approaches.Communicated by Ramaswamy H. Sarma.

  • Synthesis of Diallyl dimethyl ammonium chloride grafted polyvinyl pyrrolidone (PVP-g-DADMAC) and its applications
    Anurag Mehta, J.P. Pandey, and Gautam Sen

    Materials Science and Engineering B: Solid-State Materials for Advanced Technology, ISSN: 09215107, Volume: 263, Published: January 2021 Elsevier BV
    Abstract The search for a sustainable and nontoxic polymeric product has fired an intense research in the field of graft copolymers. This study endeavor to create a polyacrylamide free novel flocculant which showed efficient behavior towards water and wastewater treatment and for mineral ore beneficiation. We synthesized the novel graft copolymer - Poly Diallyl Dimethyl Ammonium Chloride (DADMAC) grafted polyvinyl pyrrolidone (PVP-g-pDADMAC) via graft polymerization route by microwave assisted method. The synthesized novel cationic graft co-polymer has been characterized by elemental analysis, FTIR spectroscopy, intrinsic viscosity measurement, molecular weight determination, and scanning electron micrograph (SEM), taking PVP as reference. The effects of reaction parameters onto the percentage grafting have been studied. The applicability of these grafted polymers as flocculant for the treatment of municipal sewage waste water as well as for mineral ore beneficiation and for suppression of nanopollutant like multi-walled carbon Nano-tubes (MWCNTs) (as nano scavenger) from water systems were also investigated.

  • Graft copolymer of PVP—A sutureless, haemostatic bioadhesive for wound healing application
    Pinki Pal, Naresh Rangra, S. Samanta, Ayush Aryan, Jay Prakash Pandey, and Gautam Sen

    Polymer Bulletin, ISSN: 01700839, eISSN: 14362449, Pages: 5191-5212, Published: 1 October 2020 Springer Science and Business Media LLC
    This study includes the application of graft copolymer (polyacrylamide-grafted polyvinyl pyrrolidone—PVP-g-PAM) as wound healing agent. It is hypothesized that the branches of the graft copolymer adsorb at various colloidal components of blood plasma—thus assisting in formation of a networked structure, i.e. ‘blood clot’. Further, the adhesive property of graft copolymer supports in wound closure and immobilization of pathogens. Wound healing efficiency of PVP-g-PAM has been investigated ‘in vivo’ by excision and incision wound model. The 100% wound contraction has been shown by PVP-g-PAM after 13 days. This is superior to both in cases of standard drug and ungrafted polyvinyl pyrrolidone (PVP), which fails to achieve complete wound closure even after 21 days. Additionally, the graft copolymer-treated mice’s skin exhibited higher tensile strength than that of standard drug, PVP and stitched model.

  • Gum ghatti based hydrogel: Microwave synthesis, characterization, 5-Fluorouracil encapsulation and ‘in vitro’ drug release evaluation
    Pinki Pal, Srikant Kumar Singh, Sumit Mishra, Jay Prakash Pandey, and Gautam Sen

    Carbohydrate Polymers, ISSN: 01448617, Volume: 222, Published: 15 October 2019 Elsevier BV
    The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated. Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique. The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method. The degree of crosslinking analysed through equilibrium swelling ratio (ESR) has been correlated with release kinetics. The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values. Additionally, the release rate rises with rise in pH milieu.

  • Processing techniques or mycotoxins-a balancing act of food safety and preservation
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen

    Preservatives and Preservation Approaches in Beverages: Volume 15: The Science of Beverages, Pages: 375-425, Published: 1 January 2019 Elsevier
    Abstract Mycotoxins, the toxic metabolites of filamentous fungi have deleterious and persistent effects not only on human being but also on other vertebrates and invertebrates including plants and microorganisms. Agricultural commodities may easily get contaminated during both preharvest and postharvest. Presently, over 300 mycotoxins are identified. The International Agency for Research on Cancer (IARC) has categorized some of these toxins as carcinogenic such as aflatoxin, ochratoxins, patulin, alternaria, etc. Numerous techniques are being employed in food industries for the processing and preservation of vegetables and fruits juice. The prime objective of these techniques is the development of new products and increasing the shelf life of raw and processed food products. Prevailing trends of consumption of fruits juice have compelled companies to package fruits juice with minimal treatment, but every raw is not holistic—specially when consumed in hours or even in days after packaging. The threat of mycotoxins looms covertly behind such products. The key focus of this review is to evaluate the preservation and processing techniques and their calculated risks involved with respect to uncertainty of the presence of mycotoxins.

  • Novel Biocide Based on Cationic Derivative of Psyllium: Surface Modification and Antibacterial Activity
    Pinki Pal, Aparna Banerjee, Karuna Soren, Priyanka Chakraborty, Jay Prakash Pandey, Gautam Sen, and Rajib Bandopadhyay

    Journal of Polymers and the Environment, ISSN: 15662543, Published: 2019 Springer Science and Business Media LLC
    To circumvent the problems (such as volatilization, photolytic decomposition, chemical instability, easy permeability through the skin) associated with low molecular weight antimicrobial agents, our strategy employed graft copolymerization of cationic monomer with the polymeric substrate viz. psyllium husk. The surface graft concentration of quaternary ammonium ion on psyllium was optimized by measuring the extent of grafting in the microwave-induced process. The surface modification of graft copolymers was evident by different physico–chemical techniques like 13C nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), X-ray diffractometer (XRD), differential scanning calorimetry (DSC), Thermo gravimeteric analysis (TGA), and viscometric study. The synthesized water soluble product resulted good antibacterial activity against Gram positive Staphylococcus aureus and Bacillus anthracis but failed to produce any antibacterial activity against Gram negative Salmonella typhi and Escherichia coli. The reason to failure has been explained on the basis of differences in the electrokinetic property between Gram-positive and Gram-negative bacteria. Optimum minimum inhibitory concentration (MIC) for the polymeric grade which showed good biocidal effect evaluated by both zone of inhibition and absorbance is found at 1000 µg/ml.

  • Free radical scavenging potential of sericin obtained from various ecoraces of tasar cocoons and its cosmeceuticals implication
    K. Jena, J.P. Pandey, Ruchi Kumari, A.K. Sinha, V.P. Gupta, and G.P. Singh

    International Journal of Biological Macromolecules, ISSN: 01418130, eISSN: 18790003, Volume: 120, Pages: 255-262, Published: December 2018 Elsevier BV
    Tropical tasar silkworm Antheraea mylitta is a wild sericigenous insect which is distributed in different geographical regions and named as different ecoraces. In the present study, we investigated the molecular characterisation and cosmeceutical properties of sericin extracted from different ecoraces of tasar cocoons. The surface morphology and molecular weight of cocoons were determined by scanning electron microscope (SEM) and SDS-PAGE, respectively. Characterisation of sericin was performed by various methods such as FTIR, CHNS, TGA and amino acid analyzer. The anti-tyrosinase, anti-elastase, glutathione-S-transferase inhibition, free radical scavenging potential and inhibition of oxidative damages were measured in tasar ecoraces sericin. SEM images have revealed the removal of sericin from the surface of cocoons. SDS-PAGE of sericin depicted the presence of diverse molecular weight of proteins. Structural determination by FTIR revealed the presence of both α-helical and β-sheet structures. Thermal properties of sericin were studied by TGA which showed a 50% weight loss at temperature 410 °C-430 °C. Additionally, ecoraces sericin contains 17 amino acids in which serine, aspartic acid and glycine are predominantly present (55.68-59.61%). Further, anti-tyrosinase, anti-elastase, inhibition of glutathione-S-transferase activity, free radical scavenging potential and inhibition of lipid peroxidation were also observed in ecoraces sericin. Our findings suggest that the present study appear to be helpful in exploiting sericin as potential biomaterial in cosmeceutical and allied field.

  • Synthesis and optimization of hydrolyzed gum ghatti as nano-hunters – Flocculant for destabilization of nanoparticles
    Pinki Pal, Sakshi Suman, Arpita Verma, Jay Prakash Pandey, and Gautam Sen

    Colloids and Surfaces A: Physicochemical and Engineering Aspects, ISSN: 09277757, eISSN: 18734359, Volume: 555, Pages: 699-707, Published: 20 October 2018 Elsevier BV
    Abstract This article presents a successful endeavour towards destabilization of an aqueous suspension of carbon nanoparticles via synthesized hydrolyzed graft copolymer (Hyd. GGI-g-PAM) of gum ghatti. Flocculation proficiency of the preformed graft copolymer of gum ghatti (GGI-g-PAM) has been amplified via partial alkaline hydrolysis that helps in straightening and uncoiling of the grafted monomer chains consequently increasing radius of gyration and hence the hydrodynamic volume of the molecule. Standard jar test procedure has been followed to analyze and compare the flocculation proficiency of the novel product with that of both the gum ghatti and GGI-g-PAM in coal-fine suspension as well as in aqueous suspensions of MWCNTs.

  • Synthesis and study of hydrolyzed polyacrylamide grafted polyvinyl pyrrolidone (Hyd.PVP-g-PAM) as flocculant for removal of nanoparticles from aqueous system
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen

    Materials Science and Engineering B: Solid-State Materials for Advanced Technology, ISSN: 09215107, Volume: 236-237, Pages: 32-42, Published: October 2018 Elsevier BV
    Abstract This study investigates the real potential of synthesized hydrolyzed graft copolymer (Hyd.PVP-g-PAM) as flocculant in destabilization of aqueous suspension of nanoparticles (multi-walled carbon nanotubes or MWCNTs). A novel product (Hyd.PVP-g-PAM) has been synthesized by partial alkaline hydrolysis of a preformed graft copolymer of polyvinyl pyrrolidone (PVP-g-PAM). This novel product has much higher flocculation efficacy than the parent graft copolymer (PVP-g-PAM) as well as PVP, due to much larger radius of gyration as a consequence of straightening and uncoiling of the grafted chains. The flocculation competency of the product has been examined and compared with the PVP-g-PAM and with the commercial flocculant polyacrylamide (PAM) in coal-fine and in aqueous suspensions of MWCNTs through standard jar test procedure. The result indicates that the synthesized flocculant has better flocculation efficacy than PAM. The optimal dosage as flocculant of synthesized product was at 1 ppm in MWCNT suspension whereas in coal-fine at 0.75 ppm.

  • Conferring Antibacterial Properties on Sesbania Gum via Microwave-Assisted Graft Copolymerization of DADMAC
    Pinki Pal, Aparna Banerjee, Urmi Halder, Jai Prakash Pandey, Gautam Sen, and Rajib Bandopadhyay

    Journal of Polymers and the Environment, ISSN: 15662543, Pages: 3272-3282, Published: 1 August 2018 Springer Science and Business Media LLC
    Microbial contamination, the consequences of both anthropogenic and geogenic activity, includes high health and environmental threats and may adversely affects the ecological niche. The present work is a successful attempt towards the fabrication of anti-bacterial agent by incorporation of cationic monomer, diallyldimethylammonium chloride (DADMAC) on sesbania gum through microwave assisted method. The grafted chains of poly (DADMAC) provide positive charge to sesbania gum which in turn confers antibacterial activity. The synthesized product was characterized through FTIR, SEM, TGA, DSC elemental and viscometric analysis. The inhibitory activity of the synthesized product has been evaluated in vitro against both Gram-positive (Staphylococcus aureus NCIM 2122 and Bacillus anthracis) as well as Gram-negative (Salmonella typhi NCIM 2501 and Escherichia coli NCIM 2832) bacteria through standard agar cup method. Optimum minimum inhibitory concentration for each grade of graft copolymer measured by calculating the zone of inhibition has been found at 750 µg/ml.Graphical Abstract

  • Grafted sesbania gum: A novel derivative for sugarcane juice clarification
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen

    International Journal of Biological Macromolecules, ISSN: 01418130, eISSN: 18790003, Volume: 114, Pages: 349-356, Published: 15 July 2018 Elsevier BV
    This work describes the significance of graft copolymer induced flocculation process in sugarcane juice clarification. The naturally occurring cinnamic acid has been successfully incorporated into the structure of sesbania gum through microwave assisted technique (hybrid of conventional and microwave based method) to develop novel, non-toxic, eco-friendly product with highly extended structure. The customized structure of gum derivative has been characterized by a number of modern techniques like 13C NMR, FTIR, SEM, XRD, TGA, DSC, elemental and viscometric analysis. The separation of suspended colloidal impurities from cane juice has been achieved through the application of synthesized gum derivative as a flocculant. The clarification performance of the graft copolymer has been evaluated in terms of turbidity, absorbance, and degree brix (°bx). The flocculant exhibited maximum efficiency at 1.0ppm (optimized dose) without altering the nutritional parameters (% sugar content). The clarified juice thus obtained may be used as a beverage for direct consumption or for the manufacture of white sugar.

  • Tasar silk fiber waste sericin: New source for anti-elastase, anti-tyrosinase and anti-oxidant compounds
    K. Jena, J.P. Pandey, Ruchi Kumari, A.K. Sinha, V.P. Gupta, and G.P. Singh

    International Journal of Biological Macromolecules, ISSN: 01418130, eISSN: 18790003, Volume: 114, Pages: 1102-1108, Published: 15 July 2018 Elsevier BV
    The present study investigates the properties of sericin extracted from tasar silk fiber waste (TSFW). The surface morphology of TSFW was observed by scanning electron microscope (SEM). SEM images revealed the removal of residual sericin over the surface of TSFW. The molecular weight distribution of sericin was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results suggested that TSFW sericin represented a family of proteins with wide-ranging molecular weight distribution (11-245 kDa). Structural determination by FTIR revealed the presence of both α-helical and β-sheet structures. The colour was studied by colorimeter indicating less brightness, more red and yellow colour intensities. The carbon: nitrogen ratio (C:N) was studied by CHNS element analyzer and the ratio is 5.15-7.85. Thermal properties of TSFW sericin have been studied by thermogravimetric analysis (TGA) method. TGA curve showed higher thermal stability and variable degradation profiles. Furthermore, TSFW sericin contains 17 amino acids where serine, aspartic acid and glycine are the more significant compounds (54.34-60.49%). In addition, sericin was found to inhibit tyrosinase, elastase and glutathione-S-transferase activity, and had apparent radical scavenging impacts on 2.2‑diphenyl‑1‑picryl‑hydrazil (DPPH), hydrogen peroxide and inhibition of lipid peroxidation. Result suggested that TSFW sericins might be a valuable ingredient for cosmoceutical products.

  • Sesbania gum based hydrogel as platform for sustained drug delivery: An ‘in vitro’ study of 5-Fu release
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen

    International Journal of Biological Macromolecules, ISSN: 01418130, eISSN: 18790003, Volume: 113, Pages: 1116-1124, Published: 1 July 2018 Elsevier BV
    The purpose of this study is to fabricate 5-Fluorouracil sustained release matrix based on a novel, nontoxic, eco-friendly modified biopolymer. The sesbania gum based hydrogel has been prepared by microwave assisted method using acrylamide as a monomer and N,N Methylenebisacrylamide as a crosslinker. The crosslink copolymerization has been confirmed by several modern techniques such as FTIR, SEM, XRD, TGA, DSC, elemental analysis etc. The bioactive 5-Fluorouracil has been encapsulated via solvent swelling method and its release rate has been investigated in various pH dissolution medium through USP standard protocol. The synthesized hydrogel with higher degree of crosslinking exhibited slower release rate than that of hydrogel having lower degree of crosslinking. Thus, resulting higher t25 value, the release rate increases with increase in pH of the medium. Release kinetics suggests the non-Fickian release behaviour of the hydrogel.

  • Biosorptive removal of arsenite and arsenate from aqueous medium using low-cost adsorbent derived from ‘Pods of green peas’: Exploration of kinetics, thermodynamics and adsorption isotherms
    Ashish Kumar, Jayprakash Pandey, and Satish Kumar

    Korean Journal of Chemical Engineering, ISSN: 02561115, Pages: 456-469, Published: 1 February 2018 Springer Science and Business Media LLC
    The present work explores the biosorption characteristics of pods of green peas (PGP) for arsenite [As(III)] and arsenate [As(V)] removal from aqueous medium. Optimization of adsorption parameters like contact time, arsenic concentration, temperature, biosorbent dose and pH through batch mode of experiments was studied. Among the different isotherm models, Langmuir for As(III) and Temkin for As(V) provided the best fit for the obtained data of arsenic sorption onto PGP. D-R mean free energy (E) indicates the process is physisorption. Thermodynamics studies were found to be endothermic, feasible and spontaneous. Kinetically, adsorption follows the pseudo-second-order kinetics in sorption of As(III) and As(V) both. The desorption studies of exhausted PGP show 81% of As(III) and 72% of As(V) could be leached out. Reusability of biosorbent up to 7th cycles of incessant operation supports their commercial importance with very little effects of common ions on sorption capacity.

  • Study of biochemical changes on freeze-dried and conventionally dried white button mushroom as a sustainable method of food preservation
    Pinki Pal, A. K. Singh, Dipti Kumari, Rahul Rahul, J. P. Pandey, and Gautam Sen

    Applications of Biotechnology for Sustainable Development, Pages: 149-155, Published: 6 October 2017 Springer Singapore
    The conventional biotechnology and the advanced agricultural practices may not be sufficient to meet the rising and expected high protein demands. Presently mushroom has occupied a high status because of its nutritive value, i.e. it is rich in protein and fibre along with several vitamins and minerals contents, it contains virtually no fat and cholesterol. However, preservation of mushroom during its storage and distribution has always been a challenge. Cryogenic freezing of food material is the only valuable option for its preservation. The principle behind the freeze drying involves the triple point of water. It occurs at 0 °C temperature and 4.7 mm of Hg pressure. Dehydration of food material has taken place by sublimation process. A comparative study on freeze-dried and other conventional dried mushrooms has been performed. White button mushrooms were preserved by hot air oven method and freeze-drying method. Nutritional parameters like moisture, protein, crude fat, reducing sugars, ash content, crude fibre, ascorbic acid content and sensory evaluation like texture, flavour, aroma, shape, rehydration ratio, particle nature and storage stability of samples dried by different methods have been studied by different chemical methods. The freeze-drying technique has been found excellent for mushroom dehydration, since the quality of product is found to be the best in terms of its colour, flavour, texture and nutritive value. Therefore, this method is found to be useful for preservation of mushrooms.

  • Mitigation of arsenic poisoning from drinking water by phytoremediation
    Pharma Times, ISSN: 00316849, Pages: 17-19, Published: September 2017

  • Modified PVP based hydrogel: Synthesis, characterization and application in selective abstraction of metal ions from water
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen

    Materials Chemistry and Physics, ISSN: 02540584, Volume: 194, Pages: 261-273, Published: 15 June 2017 Elsevier BV
    Abstract Graft copolymerization and simultaneous crosslinking of polyvinyl pyrrolidone (PVP) have been carried out by microwave assisted method. The pore size and thus the functional properties of hydrogels were customized exclusively for Cr(VI) and As(III) ions abstraction by varying the concentrations of monomer and crosslinker, thus enabling selective entrapment. The hydrogels have been characterized through ESR at different temperatures and pH and also through FTIR, TGA, SEM, Elemental analysis. Selectivity of hydrogels for Cr(VI) and As(III) ions abstraction from spiked water samples have been evaluated in presence of Na+, Ca2+ and Mg2+ ions at different contact times. The results indicate that hydrogels have selective capability for Cr (VI) and As (III) ion abstraction from water without abstraction of other ions present in water like Ca2+ and Mg2+ ions (natural and essential ions in potable water). The experimental equilibrium data have been analyzed by Langmuir isotherm model and the characteristic parameters for the isotherm have been evaluated. The result of rate kinetics for the entrapment of CrVI ions have been found best fitted with pseudo-second order kinetics model.

  • Synthesis, characterization and flocculation studies of a novel graft copolymer towards destabilization of carbon nano-tubes from effluent
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen

    Polymer, ISSN: 00323861, Volume: 112, Pages: 159-168, Published: 10 March 2017 Elsevier BV
    Abstract Nano-particles discharged through anthropogenic activities contaminate the hydrosphere which create complications include bioaccumulation and bio-toxicity. Destabilization of nano-particles suspension is a challenging task. The present study is a successful attempt towards destabilization of aqueous suspension of multi-walled carbon Nano-tubes (MWCNTs) by a functionalized cationic polymeric flocculant. The flocculant was fabricated by microwave-assisted polymerization of diallyldimethylammonium chloride (DADMAC) on gum tragacanth. The grafted chains of poly(DADMAC) enhance the water solubility and functionality of the synthesized graft copolymer. The flocculation efficacy of the product has been studied in kaolin suspension and in destabilization of aqueous suspension of MWCNTs through standard jar test procedure. The optimal dosage of GT-g-P(DADMAC) as flocculant in MWCNTs suspension has been found at 1.875 ppm and in kaolin it was 1.25 ppm.

  • Synthesis of polyacrylamide grafted polyvinyl pyrollidone (PVP-g-PAM) and study of its application in algal biomass harvesting
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen

    Ecological Engineering, ISSN: 09258574, Volume: 100, Pages: 19-27, Published: 1 March 2017 Elsevier BV
    Abstract Acrylamide monomers have been grafted onto the backbone of polyvinyl pyrollolidone by microwave assisted process, resulting to a novel graft copolymer i.e. polyacrylamide grafted polyvinyl pyrolidone (PVP-g-PAM).The synthesized graft copolymer have been characterized through standard physicochemical techniques. Further, dewatering capability of distinct grades of PVP-g-PAM has been studied as a flocculant in algal biomass harvesting. Best grade of graft copolymer showed highest flocculation efficacy which collected 35.37% algal biomass (speculative, as indicated by optical density) and 47.82% by gravimetric method (actual biomass recovered). The microscopic images of collected biomass indicated that the algal cells were vital and unbroken but stuck together in a massive clump which was collected by decantation process. Some nutritional investigations of the collected biomass were also carried out. The protein and crude fat content in dry algal biomass has been found 39.46% and 32.0% respectively. The calculated calorific value of algal biomass by DULONG FORMULA has been found 3591.26 Kcal/Kg.

  • Synthesis and application as programmable water soluble adhesive of polyacrylamide grafted gum tragacanth (GT-G-PAM)
    Pinki Pal, Jay Prakash Pandey, and Gautam Sen

    Biopolymer Grafting: Applications, Pages: 153-203, Published: 1 January 2017 Elsevier
    Abstract A novel graft copolymer–polyacrylamide grafted gum tragacanth (GT-g-PAM) was synthesized by microwave assisted method, i.e., grafting initiated by a synergism of chemical free radical initiator and microwave radiation. The synthesized graft copolymer has been characterized through various physicochemical techniques such as intrinsic viscosity measurement, fourier transform infrared spectroscopy, analysis of constituent elements, surface morphological study, and thermal analysis. The synthesized product has been examined as an adhesive through standard shear load test of wooden blocks conjoined through the synthesized graft copolymer as an adhesive. The programmability of properties in terms of percentage grafting of the novel graft copolymer as a water-soluble adhesive was investigated.


1. Anurag Mehata, J.P. Pandey, Gautam Sen; Synthesis of diallyl dimethyl ammonium chloride grafted polyvinyl pyrrolidone (PVP-g-DADMAC) and its applications, Material Science & Engineering B, Vol.263,114750(2020).
2. Pinki pal, Srikant kumar singh, Sumit Mishra, Jay prakash pandey, Gautam sen; Gum ghatti based hydrogel: Microwave synthesis, characterization, 5- Fluorouracil encapsulation and ‘in vitro’ drug release evaluation, Carbohydrate polymers, Vol.222,114979(2019).
3. Pinki pal, Naresh Ranga, S.Samanta, Ayush Aryan, Jay Prakash Pandey, Gautam Sen; Graft copolymer of PVP-A sutureless, hameostatic bioadhesive for wound healing applications, Polymer Bulletein, Vol.76,pp.5191-5212(2019).
4.Priti Rani, Pinki Pal, Jay Prakash Pandey, Sumit Mishra, Gautam Sen; Alginic Acid Derivatives: Synthesis, Characterization and Application in Wastewater Treatment, Journal of polymer and environment, Vol.21, pp.2759-2783(2019).
5. Pinki pal, Aparna Banerjee, Karuna Soren,Priyanka Chakraborty, Jay Prakash Pandey, Gautam Sen, Rajib Bandopadhay; Novel Biocide Based on Cationic Derivative of Psyllium: Surface Modification and Antibacterial Activity, Journal of polymer and the environment, Vol.27,pp.1178-1190(2019).
7. Pinki Pal, Jay Prakash Pandey, Gautam Sen; Synthesis and study of hydrolyzed polyacrylamide grafted polyvinyl pyrrolidone (Hyd. PVP-g-PAM) as flocculant for removal of nanoparticles from aqueous system, Material Science & Engineering B, Vol.236, pp.32-42(2018)