Saad Shakir Mahdi AL-Amara
@uobasrah.edu.iq
Dpartment of biology
University of Basrah
Scopus Publications
Scopus Publications
Amani A. Al-Abdullah, Saad S.M Al-Amara, and Shayma’a J. Raisan
Egyptian Knowledge Bank
MURTAKAB Y. AL-HEJJAJ, YASIR A.J. ALABDALI, YESSAR A. DAWOOD, SAAD S. AL-AMARA, and SHAYMA’A J. RAISAN
Egyptian Knowledge Bank
A. Ali Albadery, S. Shakir Mahdi Al-Amara and A. Abd-Al-Ridha Al-Abdullah
One of the most important nosocomial organisms that cause urinary tract infections (UTIs) in cancer patients is Escherichia coli. A significant cause of concern in managing UTIs is the development of carbapenem-resistant bacteria. Escherichia coli with carbapenem resistance has become a more serious problem, particularly in Iraq. In this regard, the present study aimed to estimate the prevalence of carbapenem-resistant E. coli in Al-Basrah, Iraq. Conventional tests and the Vitek®2 system were used to identify the isolates and determine the susceptibility of E.coli isolates to antimicrobials. In addition, E.coli isolates were tested by mCIM and eCIM methods. Moreover, the major carbapenemase genes, including blaSPM, blaIMP, blaVIM, and blaKPC were detected by polymerase chain reaction. In total, 120 urine samples were collected from cancer patients who were suspected of having urinary tract infections at Basrah Center of Oncology Al-Sader Teaching Hospital, Basrah, Iraq. Identification of bacterial growth by using biochemical tests revealed different bacterial species. The most frequent bacteria were E. coli (n=22, 53.65%) isolates. The results showed that 13 (59.09%) and 11 (50%) out of 22 E. coli isolates were positive for the production of carbapenemase, based on the eCIM and sCIM, respectively. The majority of E.coli in this study possessed the blaVIM gene (n=13, 59.1%), followed by the blaKPC gene (n=5, 22.73%), blaIMP gene (n=5, 22.73%), and blaSPM gene (n=4, 18.18%). There is a spread of more than one type of carbapenemase among the E. coli isolates collected from UTI cancer patients in Basrah Hospital. The E. coli identified in the current study had a strong capacity to produce carbapenemase enzymes against the four generations of antibiotics, including imipenem and meropenem antibiotics.
Murtakab Y Al-Hejjaj, Saad S Al-Amara, Yessar A Dawood, Shaymaa Jabbar Raisan, and Hassan M AlTameemi
Africa Health Research Organization
Bacterial contamination is an important indicator for soil quality that could be related to the plant’s suitability for animals ‘grazing and ultimately human public health. Consequently, the microbial characteristics of soil could be linked to animals’ products quality, such as milk and meat. Bacillus species is one of the most distributed soil contaminating bacteria and is linked to animals’ products quality. Three different grazing areas (Abo Al-Kasib, Al-Seba, and Al-Fawdistricts) in Basrah province were selected to determine the bacterial (Bacillus)distribution in soil. Samples were randomly collected from each field of the study. Based on the bacterial cell shape and staining (microscopy), colony morphology, biochemical tests, and the 16S rRNA genes amplification results, the bacterial isolates were identified to be from the genus Bacillus, of which Bacillus cereus was dominant in all samples. Five new isolates were identified based on 16S rRNA nucleotide sequences. Phylogenic analysis showed a close relation between three isolates to Bacillus cereuswith high 16S rRNA gene sequences similarity 99% (SAMU1), 99% (SAMU2) and 98.9% (SAMU3). Furthermore, two isolates showed sequences similarity to Bacillus paramycoides (99%, SAMU4) and Bacillus safensis (99%, SAMU5). All the above isolates were registered in the NCBI centre under the following names Bacillus cereus strain SAMU1, Bacillus cereus strain SAMU2,Bacillus cereus strain SAMU3,Bacillus paramycoidess strain SAMU4 and Bacillus safensis strain SAMU5 and were assigned the following accession numbers MK418732, MK490900, MK490901, MK490902,and MK490903respectively.In addition, some isolates were found to be 100% similar to the already identified Bacillus strains such as Bacillus safensis, B. pummilus, B. paramycoides and B. safensis. The current study investigated and mapped the distribution of Bacillus Spp in animals grazing areas that had been subjected to salinity increment.
Shayma'a Jabbar Raisan, Huda Kadhim Kareem, and Saad S. Mahdi Al-Amara
Africa Health Research Organization
This study aimed to determine total IgE in subjects under study and controls an estimation of the specific IgE antibody response against kiwifruit antigen by ELISA assay. There are many studies on against kiwifruit in other countries of the world, but this study differs in sample type and Geographical location. A total of one hundred twenty blood samples from allergic patients (41 Males and 55 female) with age group (15-69) years were tested by direct and indirect Enzyme-linked immunosorbent assay for total and specific IgE antibodies against kiwifruit antigens. In our result, we show that the total IgE 100>IU/ml for patients had a higher rate of (72.9%) with significant difference (p<0.01) between total IgE 100< IU/ml, the sera positivity against kiwifruit had a higher rate of (72.9 %) compared with control. Female and first age group ≥40 had a higher rate of sera positivity against allergens. At rate of (83.6%, 64.6%) respectively. Also, the rustles showed relationship between total (IgE<100IU/ml) and specific had higher rates in Sera negative kiwifruit allergens were (53.8%) compared with sera positivity against kiwi fruit allergens a rate of (27.1%) while the relationship between total (IgE>100 IU/ml) and specific IgE had higher rates in sera positivity against kiwifruit allergens at a rate of (78.6%) compared with sera negative kiwifruit allergens at a rate of (21.4%).