Yuan Su

@purdue.edu

Post-Doctoral Research Associate
Purdue University



              

https://researchid.co/yuansu
3

Scopus Publications

228

Scholar Citations

6

Scholar h-index

5

Scholar i10-index

Scopus Publications

  • The SARS-CoV-2 nucleoprotein associates with anionic lipid membranes
    Mandira Dutta, Yuan Su, Caroline B. Plescia, Gregory A. Voth, and Robert V. Stahelin

    Elsevier BV

  • SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles
    Caroline B. Plescia, Emily A. David, Dhabaleswar Patra, Ranjan Sengupta, Souad Amiar, Yuan Su, and Robert V. Stahelin

    Elsevier BV
    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first discovered in December 2019 in Wuhan, China, and expeditiously spread across the globe causing a global pandemic. Research on SARS-CoV-2, as well as the closely related SARS-CoV-1 and MERS coronaviruses, is restricted to BSL-3 facilities. Such BSL-3 classification makes SARS-CoV-2 research inaccessible to the majority of functioning research laboratories in the United States; this becomes problematic when the collective scientific effort needs to be focused on such in the face of a pandemic. However, a minimal system capable of recapitulating different steps of the viral life cycle without using the virus’ genetic material could increase accessibility. In this work, we assessed the four structural proteins from SARS-CoV-2 for their ability to form virus-like particles (VLPs) from human cells to form a competent system for BSL-2 studies of SARS-CoV-2. Herein, we provide methods and resources of producing, purifying, fluorescently and APEX2-labeling of SARS-CoV-2 VLPs for the evaluation of mechanisms of viral budding and entry as well as assessment of drug inhibitors under BSL-2 conditions. These systems should be useful to those looking to circumvent BSL-3 work with SARS-CoV-2 yet study the mechanisms by which SARS-CoV-2 enters and exits human cells.

  • The minor matrix protein VP24 from ebola virus lacks direct lipid-binding properties
    Yuan Su and Robert V. Stahelin

    MDPI AG
    Viral protein 24 (VP24) from Ebola virus (EBOV) was first recognized as a minor matrix protein that associates with cellular membranes. However, more recent studies shed light on its roles in inhibiting viral genome transcription and replication, facilitating nucleocapsid assembly and transport, and interfering with immune responses in host cells through downregulation of interferon (IFN)-activated genes. Thus, whether VP24 is a peripheral protein with lipid-binding ability for matrix layer recruitment has not been explored. Here, we examined the lipid-binding ability of VP24 with a number of lipid-binding assays. The results indicated that VP24 lacked the ability to associate with lipids tested regardless of VP24 posttranslational modifications. We further demonstrate that the presence of the EBOV major matrix protein VP40 did not promote VP24 membrane association in vitro or in cells. Further, no protein–protein interactions between VP24 and VP40 were detected by co-immunoprecipitation. Confocal imaging and cellular membrane fractionation analyses in human cells suggested VP24 did not specifically localize at the plasma membrane inner leaflet. Overall, we provide evidence that EBOV VP24 is not a lipid-binding protein and its presence in the viral matrix layer is likely not dependent on direct lipid interactions.

RECENT SCHOLAR PUBLICATIONS

  • ILP1 and NTR1 affect the stability of U6 snRNA during spliceosome complex disassembly in Arabidopsis
    J Wu, W Chen, S Ge, X Liu, J Shan, M Zhang, Y Su, Y Liu
    Plant Science 347, 112199 2024

  • Spliceosomal snRNAs, the Essential Players in pre‐mRNA Processing in Eukaryotic Nucleus: From Biogenesis to Functions and Spatiotemporal Characteristics
    Y Su, J Wu, W Chen, J Shan, D Chen, G Zhu, S Ge, Y Liu
    Advanced Biology, 2400006 2024

  • R3-MYB proteins OsTCL1 and OsTCL2 modulate seed germination via dual pathways in rice
    Y Yi, C Lin, X Peng, M Zhang, J Wu, C Meng, S Ge, Y Liu, Y Su
    The Crop Journal 11 (6), 1752-1761 2023

  • Phosphate deficiency modifies lipid composition and seed oil production in camelina
    J Li, Y Su, CA Shapiro, DP Schachtman, X Wang
    Plant Science 330, 111636 2023

  • Phospholipase Dα6 and phosphatidic acid regulate gibberellin signaling in rice
    H Cao, R Gong, S Yuan, Y Su, W Lv, Y Zhou, Q Zhang, X Deng, P Tong, ...
    EMBO reports 22 (10), e51871 2021

  • Different effects of phospholipase Dζ2 and non‐specific phospholipase C4 on lipid remodeling and root hair growth in Arabidopsis response to phosphate deficiency
    Y Su, M Li, L Guo, X Wang
    The Plant Journal 94 (2), 315-326 2018

  • Roles of Phospholipases and Ribosomal S6 Kinase in Lipid Remodeling and Growth in Arabidopsis Response to Phosphate Deprivation
    Y Su
    University of Missouri-Saint Louis 2018

  • Non‐specific phospholipase C1 affects silicon distribution and mechanical strength in stem nodes of rice
    H Cao, L Zhuo, Y Su, L Sun, X Wang
    The Plant Journal 86 (4), 308-321 2016

  • Phosphatidic acid as lipid messenger and growth regulators in plants
    X Wang, Y Su, Y Liu, SC Kim, B Fanella
    Phospholipases in plant signaling, 69-92 2014

  • Phosphatidic acid-mediated signaling
    Y Liu, Y Su, X Wang
    Lipid-mediated protein signaling, 159-176 2013

MOST CITED SCHOLAR PUBLICATIONS

  • Phosphatidic acid-mediated signaling
    Y Liu, Y Su, X Wang
    Lipid-mediated protein signaling, 159-176 2013
    Citations: 110

  • Different effects of phospholipase Dζ2 and non‐specific phospholipase C4 on lipid remodeling and root hair growth in Arabidopsis response to phosphate deficiency
    Y Su, M Li, L Guo, X Wang
    The Plant Journal 94 (2), 315-326 2018
    Citations: 51

  • Non‐specific phospholipase C1 affects silicon distribution and mechanical strength in stem nodes of rice
    H Cao, L Zhuo, Y Su, L Sun, X Wang
    The Plant Journal 86 (4), 308-321 2016
    Citations: 30

  • Phosphatidic acid as lipid messenger and growth regulators in plants
    X Wang, Y Su, Y Liu, SC Kim, B Fanella
    Phospholipases in plant signaling, 69-92 2014
    Citations: 18

  • Phospholipase Dα6 and phosphatidic acid regulate gibberellin signaling in rice
    H Cao, R Gong, S Yuan, Y Su, W Lv, Y Zhou, Q Zhang, X Deng, P Tong, ...
    EMBO reports 22 (10), e51871 2021
    Citations: 11

  • Phosphate deficiency modifies lipid composition and seed oil production in camelina
    J Li, Y Su, CA Shapiro, DP Schachtman, X Wang
    Plant Science 330, 111636 2023
    Citations: 8