Dr. Chandrashekar Mootapally
@gtu.ac.in
School of Applied Sciences and Technology
Gujarat Technological University
RESEARCH INTERESTS
Marinomics (pro- to eu- karyotes), Population Genetics and Molecular Taxonomy, Biotechnology, Bioinformatics.
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Parthkumar Prajapati, Riya Desai, Mamta Varma, Ketankumar Panchal, Subhash Jakhesara, Prakash Koringa, Vaibhav Bhatt, Neelam Nathani, and Chandrashekar Mootapally
Springer Science and Business Media LLC
Divyarajsinh A. Jadeja, Neelam M. Nathani, Chandrashekar Mootapally, and Ramesh K. Kothari
Springer Science and Business Media LLC
Neelam M. Nathani, Chandrashekar Mootapally, Parth Sharma, Solly Solomon, Rakesh Kumar, Abhay B. Fulke, and Manish Kumar
Elsevier BV
Rahul Silori, Vikalp Shrivastava, Payal Mazumder, Chandrashekar Mootapally, Ashok Pandey, and Manish Kumar
Elsevier BV
Shilpa Doultani, Vishal S. Suthar, Chandrashekhar Mootapally, Neelam Nathani, Madhavi Joshi, Deepak B. Patil, and Chaitanya G. Joshi
Agricultural Research Communication Center
Background: Number of genes expressed during in vitro maturation (IVM) of which selected genes can be used as the potential biomarkers of oocyte competence. Hence, this study was planned to evaluate selected gene expression of GDF9, HAS2, SPRY, ARHGAP22, COL18A1 and GPC4 in IVM and immature cumulus oocyte complexes (COCs). Methods: The COCs were recovered from slaughter origin ovaries of buffaloes. Of which first three grade COCs were randomly allotted in immature (IMT; n=217) and IVM group (n=272). IVM of COCs was performed under drops of BO-maturation media in CO2 incubator at 39.0°C for 24 hours. The expression of genes was evaluated using qPCR and the relative expression of each gene was calculated using the ΔΔCt method with efficiency correction. The logarithmic transformation of fold change of each candidate genes in the IVM group was computed against the IMT group using the Ct values. Result: The expression obtained for IVM group of earlier reported up-regulated genes (GDF9, HAS2, SPRY1) was higher (upto 10xfold) compared to the IMT group. Relatively lower expression was observed for the rest of the three candidate genes (ARHGAP22, COL18A1, GPC4) in the bovine transcripts of oocyte which were earlier also reported as down regulated.
Neelam M. Nathani, Kaushambee J. Dave, Priyanka P. Vatsa, Mayur S. Mahajan, Parth Sharma, and Chandrashekar Mootapally
Springer Science and Business Media LLC
Neelam M. Nathani, Kaushambee J. Dave, Priyanka P. Vatsa, Mayur S. Mahajan, Parth Sharma, and Chandrashekar Mootapally
Springer Science and Business Media LLC
AbstractProkaryoplankton genomes from the deep marine sediments are less explored compared to shallow shore sediments. The Gulfs of Kathiawar peninsula experience varied currents and inputs from different on-shore activities. Any perturbations would directly influence the microbiome and their normal homeostasis. Advancements in reconstructing genomes from metagenomes allows us to understand the role of individual unculturable microbes in ecological niches like the Gulf sediments. Here, we report 309 bacterial and archaeal genomes assembled from metagenomics data of deep sediments from sites in the Gulf of Khambhat and Gulf of Kutch as well as a sample from the Arabian Sea. Phylogenomics classified them into 5 archaeal and 18 bacterial phyla. The genomes will facilitate understanding of the physiology, adaptation and impact of on-shore anthropogenic activities on the deep sediment microbes.
Jay Nimavat, Chandrashekar Mootapally, Neelam M. Nathani, Devyani Dave, Mukesh N. Kher, Mayur S. Mahajan, Chaitanya G. Joshi, and Vaibhav D. Bhatt
Frontiers Media SA
Humankind has suffered many pandemics in history including measles, SARS, MERS, Ebola, and recently the novel Coronavirus disease caused by SARS-CoV-2. As of September 2021, it has affected over 200 million people and caused over 4 million deaths. India is the second most affected country in the world. Up to this date, more than 38 Lakh viral genomes have been submitted to public repositories like GISAID and NCBI to analyze the virus phylogeny and mutations. Here, we analyzed 2349 genome sequences of SARS-CoV-2 submitted in GISAID by a single institute pertaining to infections from the Gujarat state to know their variants and phylogenetic distributions with a major focus on the spike protein. More than 93% of the genomes had one or more mutations in the spike glycoprotein. The D614G variant in spike protein is reported to have a very high frequency of >95% globally followed by the L452R and P681R, thus getting significant attention. The antigenic propensity of a small peptide of 29 residues from 597 to 625 of the spike protein variants having D614 and G614 showed that G614 has a little higher antigenic propensity. Thus, the D614G is the cause for higher viral antigenicity, however, it has not been reported to be effective to be causing more deaths.
Neelam M. Nathani, Priyanka P. Vatsa, Mayur S. Mahajan, Solly Solomon, and Chandrashekar Mootapally
Elsevier BV
Mayur Mahajan, Devika Manek, Nishant Vora, Ramesh K. Kothari, Chandrashekar Mootapally, and Neelam M. Nathani
Elsevier BV
Chandrashekar Mootapally, Mayur S. Mahajan, and Neelam M. Nathani
Springer Science and Business Media LLC
Riddhi Rajyaguru, Nataraja Maheshala, Chandrashekar Mootapally, Neelam Nathani, Rukamsingh Tomar, Hiren Bhalani, and Priyanka Sharma
CRC Press
Ninian Prem Prashanth Pabbathi, Chandrashekar Mootapally, Aditya Velidandi, Kaushambee Dave, Neelam M. Nathani, and Rama Raju Baadhe
Elsevier BV
Chandrashekar Mootapally, Neelam Mustakali Nathani, Solly Solomon, Bharathiraja Subramanian, and Indra Ramjibhai Gadhvi
Wiley
Neelam M. Nathani, Ananya Mitra, Chandrashekar Mootapally, and Ramesh K. Kothari
Wiley
Prashakha J. Shukla, Vaibhav D. Bhatt, Jayaraman Suriya, and Chandrashekar Mootapally
Wiley
Apurvasinh Puvar, Chandrashekar Mootapally, Chaitanya Joshi, and Madhvi Joshi
Elsevier BV
C. Mootapally, I. R. Gadhvi, Bharat B. Maitreya and C. Joshi
Springer Singapore
Chandrashekar Mootapally, Neelam M. Nathani, Paresh Poriya, Imtiyaz Beleem, Jignesh C. Dabhi, Indra R. Gadhvi, and Chaitanya G. Joshi
Springer Science and Business Media LLC
AbstractAntibiotic resistance has been one of the most persistent global issue. Specifically, marine microbiomes have served as complex reservoirs of antibiotic resistant genes. Molecular advancements have enabled exploration of the uncultured microbial portion from hitherto difficult to sample niches such as deeper oceans. The Gulfs of Kathiawar Peninsula have been known for their unique properties like extreme tidal variations, different sediment textures and physicochemical variations. Pelagic sediment cores across four coordinates each of the Gulf of Kutch, Gulf of Khambhat and an open Arabian Sea were collected, processed for metagenomic sequencing and assessed for antibiotic and metal resistome. The dominant genes were mostly resistant to macrolides, glycopeptides and tetracycline drugs. Studied samples divided into three clusters based on their resistome with carA, macB, bcrA, taeA, srmB, tetA, oleC and sav1866 among the abundant genes. Samples from creek of Gulf of Kutch and mouth of Gulf of Khambhat were most diverse in resistance gene profile. Biomarkers observed include gyrA mutation conferring resistance gene in the Arabian Sea; Proteobacteria species in Gulf of Kutch and Arabian sea; while Aquificae, Acidobacteria and Firmicutes species in the Gulf of Khambhat. Region-wise differentially abundant 23 genes and 3 taxonomic biomarkers were proposed for antibiotic resistance monitoring.
Neelam M. Nathani, Chandrashekar Mootapally, and Bharti P. Dave
Elsevier BV
Ramesh K. Kothari, Neelam M. Nathani, Chandrashekar Mootapally, Jalpa K. Rank, Haren B. Gosai, Bharti P. Dave, and Chaitanya G. Joshi
Elsevier
Sabapathi Arularasan, Neelam M Nathani, Venmathi Maran BA, Ramesh K Kothari, and Chandra Shekar Mootapally
Bulletin of Marine Science
Haplotype variation and phylogenetics of three economically important morphological variants of Turbinella pyrum (Linnaeus, 1767)—var. obtusa, var. acuta, and var. globosa—were elucidated based on molecular analysis of mitochondrial 16S rDNA signatures. Haplotype distribution indicated that the var. obtusa was unique as revealed by the absence of any shared haplotype with the other two variants. Diversity indices depicted overall high mtDNA diversity for the morphological variants. Analysis of molecular variance revealed distinct population structure in the three studied variants. Based on haplotype diversity (H) and genetic distances, highest variation was observed between var. obtusa and the other two variants, while lesser variation was observed between var. acuta and var. globosa. However, highest nucleotide diversity (π) was observed between the var. acuta and the other two variants. Phylogenetic analyses also distinguished a phylogroup of var. obtusa highlighting its uniqueness. Overall, findings of the present molecularbased analysis indicated substantial genetic variation among the studied morphological variants of genus Turbinella and revealed that the morph obtusa was quite distinct from acuta and globosa.
Neelam M. Nathani, Amrutlal K. Patel, Chandra Shekar Mootapally, Ramesh K. Kothari, and Chaitanya G. Joshi
Springer Singapore
Chandra Shekar Mootapally, Paresh Poriya, Neelam Mustakali Nathani, Balu Alagar Venmathi Maran, and Indra Ramjibhai Gadhvi
Springer Singapore