professor Dr.saad hasan mohammed ali
@uobaghdad.edu.iq
COLLEGE OF MEDICINE, BAGHDAD
Scopus Publications
Scopus Publications
Sarmad Zeiny and Saad Mohammed Ali
EpiSmart Science Vector Ltd
BACKGROUND
Tumor-infiltrating lymphocytes (TIL) are important immunological components in response to cancers. Patients with higher numbers of TIL in breast cancerous tissues, comprising T- cytotoxic and T - helper cells along with B- and rare natural killer (NK) cells, have more favorable clinical outcomes.
OBJECTIVE
To analyze the rate of the expressed surface biomarker proteins of CD20-B cells and CD56- NK cells on the infiltrative lymphocytic subpopulations in a group of breast tumorous tissues (invasive and benign) from female patients in Iraq and explore the relations to the grade of the invasive breast cancerous tissues.
PATIENTS AND METHODS
One hundred and 75 archived breast tissues were enrolled in this retrospective research: 100 archived breast from female patients with invasive breast cancers (BC) [20 well differentiated BC tissues; 48 moderately differentiated BC and 32 poorly differentiated BC tissues]; 50 tissue biopsies from female patients with benign breast tumors and 25 apparently normal individuals with healthy breast tissues (included as the control group for this study). Immunohistochemistry was achieved for the detection of the expressed surface biomarker proteins related to B cell CD20 and NK cell CD56 present on the infiltrative lymphocytic subpopulations in breast tissues by using specific primary antibodies for these proteins via utilizing an immune-enzymatic antigen detection system.
RESULTS
The detection of IHC reactions for the expressed B cell CD20 - cell surface ( CD) biomarker proteins were observed in 53 out of 100 (53.0%) BC tissues, and in 24 out of 50 (48.0%) benign breast tumorous tissues, while CD20- positive cell surface markers was detected in apparently healthy breast tissues of the control group in a percentage of 32.0% (8 out of 25 tissues). Statistical significant differences (P<0.05) between both groups of malignant and benign breast tumors and the control group were found. However, between breast malignant and benign tumor groups, no significant difference was found ( p >0.05). Detection of CD56- IHC reactions revealed in 14% (14 out of 100 BC tissues), in 16% (8 out of 50 benign breast tissues) and none of control breast tissues revealed CD56- IHC reactions. Among all the enrolled groups, no significant differences (P>0.05) were detected.
CONCLUSIONS
The observed significant rates that showed highly significant differences between both studied groups of breast malignant and benign tumor in comparison to the control group indicate that the CD20- positive infiltrative B cell- lymphocytic subpopulations might contributed in the defense against these subsets of benign and malignant breast tumors. However, the observed rates of NK cell CD56 present on the lymphocytic subpopulations infiltrating the examined malignant and benign breast tumorous tissues seeming to play irrelevant roles in the defense against these studied breast tumor groups.
Saad Hasan Mohammed Ali, Khalil Ismail Abid Mohammed, Wifaq M Ali, Suha AL-Fakhar, Shakir H. Mohammed Al-Alwany, and Jinan M. Mousa
EpiSmart Science Vector Ltd
OBJECTIVE
The objective of the study was to examine the prevalence of HMTV infection, its associations with breast malignant tissues, and the expression of BRCA1 and BRCA2 proteins.
METHODS
One hundred archival breast tissues, 40 biopsies from female patients with breast cancer (BC), and 20 healthy breast tissues from the control group were used in the study. Immunohistochemical analysis was conducted to detect the expressed BRCA1 and BRCA2 proteins. Digoxigenin-labeled HMTV probes were used in chromogenic in situ hybridization for the identification of HMTV in breast tumor tissues. The complementary sequence sites of the HMTV probe sequences were stained by NBT/BCIP as blue signals.
RESULTS
There were 12 out of 40 (30%) benign breast tumorous tissues and 14 out of 40 (35%) BC tissues, while healthy control breast tissues were 10% (2 out of 20 tissues). Positive immunohistochemical (IHC) reactions for BRCA2 protein were observed in 12 out of 40 BC tissues (30.0%), 25% of benign breast tumorous tissues, and 5% of the control group. A significant (p < 0.05) statistical difference in the percentages of HMTV in the studied groups was found.
CONCLUSION
HMTV might contribute to the development of subsets of benign and malignant breast tumors. The observed rates of defective or mutated BRCA1 and BRCA2 genes in healthy tissues indicate a role in the development of breast tumors.
Nihaya Kadhim Salim, SaadHasan Mohammed Ali, and Israa Mahdi Al-Sudani
A and V Publications
Background: The incidence of thyroid cancer has increased during the last three decades. Many studies have been conducted to determine whether there is a link between viral infections and thyroid carcinogenesis. Human Papilloma viruses (HPV) are related to a variety of benign and malignant tumors. P57 is a member of the Cip/Kip family that binds and inhibits all cyclin/CDK complexes, resulting in cell-cycle arrest as well as cell growth. Objective: This retrospective study designed to investigate histopathological expression of thyroid cancer tissues in relation to the concordant HPV31/33 infection and p57 protein over-expressions. Patients and Methods: HPV31/33 DNA and P57-gene protein expression were evaluated in 116 thyroid tissues. The samples related to 36 patients with thyroid carcinoma, 40 thyroid adenoma, and 40 normal thyroid tissues. In situ hybridization (ISH) used to identify HPV31/33-DNA, whereas immunohistochemistry (IHC) used to assess P57-gene expression. Results: Regarding thyroid carcinoma group, HPV31/33DNA-ISH are responses observed in 77.8%, in 30 % of thyroid adenomas group, and in 22.5 %of normal thyroid tissues group. The difference between HPV31/33 in thyroid malignancies and the control group was statistically significant. The p57 protein detected in 75% thyroid cancer tissues and in 52.5 % adenoma thyroid tissues, whereas 35% of the investigated normal thyroid tissues showed IHC-reactions. The difference in the detected percentages of P57 protein was statistically significant in thyroid tissues between the study groupsin relations to the control group. Conclusions: The significant detection of HPV31/33 along with over-expressed P57-gene in thyroid cancer patients could support a possible role for both HPV 31/33 along with this p57 protein in thyroid pathogenesis and for carcinogenesis.
Suha A. AL-Fakhar, Wifaq M. Ali, Khalil Ismail A. Mohammed, Saad Hasan Mohammed Ali, Jinan M. Mousa, and Israa Qasim Hussein
A and V Publications
Background: Recently it has been noticed that toxoplasmosis played an important role in distressing women’s pregnancy through certain outcomes such as congenital abnormalities and abortions. The lack in Vitamin D may play role fragility of bones like; osteoporosis, and bone deformities. Objective: To evaluate the association between the lake in vitamin D and toxoplasmosis seropositivity in the women’s sera in the age of childbearing by applying ELISA method. Materials and Methods: 56 women, from Central Public Health Laboratories in Baghdad Province were selected in this study, their age range between (17-45) years. The individuals were diagnosed with BOH (Bad Obstetric History). Among 56 women infected (44)or not infected (12)with toxoplasmosis blood samples were collected from patients who visited Central Public Health Laboratories in Baghdad Province . IgG and IgM Abs Antibodies against Toxoplasma gondii were detected using minividas kit ) BioMerieux /France). Also vitamin D was detected using by ELISA microwells method (Monobind Inc. USA). Results: The results were considered as statistically significant (P=0.386), it shows no important difference between toxoplasmosis and the concentration of vitamin D3 in women with history of abortion or not. Conclusion: The toxoplasmosis rate in women in childbearing age was elevated, and the association between vitamin D deficiency and toxoplasmosis was not considerable.
Khalil Ismail A. Mohammed, Saad Hasan Mohammed Ali, Suha A. Al-Fukhar, Wifaq M. Ali Al-Wattar, and Jinan M. Mousa
Journal of Experimental Biology and Agricultural Sciences
Salmonella typhoid and paratyphoid are transmitted mainly by the fecal-oral route. This study was designed to find the correlation between Salmonella and IgM, IgG, and the levels of interleukin-33, TNF-α, and LTB 4. The study was carried out from March 2020 to January 2021 for the detection of Salmonellosis in 100 suspected patients with age group ranging from 17 - 69 years, who attended Baghdad teaching hospitals that had been examined and defined as suspected cases by a specialized physician with the recording of clinical manifestation. The diagnosis was done by immunochromatography method, a blood sample was taken from the patient as well as other 30 healthy controls matching in age and gender. The study included measurement of the level of interleukin -33, Tumor necrosis Factor α, and Leukotreins B4 level in sera of patients and healthy control. The results indicated that anti –salmonella IgM positive in 54 cases, anti- salmonella IgG positive in 46 cases, and 18 positive cases with both IgM and IgG. The Level of interleukin 33, Tumor necrosis Factor – α increased significantly while the serum Leukotreins B4 level decreased significantly in patients sera as compared with healthy control.
Tayseer Anmar Hassan, Jenan M. Jawad AL-Saffar, and Saad Hasan Mohammed Ali
University of Baghdad College of Science
Human cytomegalovirus (HCMV) infects a wide range of human cells, resulting in both benign and malignant tumors. In the last few decades, proteins and/or nucleic acids of the virus were found to be often highly expressed in in patients with basal cell hyperplasia and prostatic neoplasia.
 This research aimed to unravel the rate of HCMV infections among prostatic tissue subsets from Iraqi patients with adenocarcinoma and benign hyperplasia.
 One hundred, formalin-fixed and paraffin embedded prostatic tissues were obtained from 40 tissue samples collected from different grades of prostate carcinoma; 40 from benign prostatic hyperplasia and 20 from apparently healthy prostatic tissues. These tissue specimens were collected from the archives of different public and private histopathological laboratories in Baghdad. Detection of HCMV-DNA was achieved by a highly sensitive version of chromogenic in situ hybridization technique.
 The signals of chromogenic in situ hybridization reactions for HCMV-DNA detection in prostatic adenocarcinoma tissues were found in 65% (26 out of 40) of the tissues, whereas in BPH (Benign Prostatic Hyperplasia), HCMV-DNA was detected in 57.5% (23 out of 40) of the tissues, and in the healthy control group in 25% (5 out of 20) of the tissues. The highest percentage of positive- HCMV- DNA-CISH reactions (57.5%) was found in prostatic adenocarcinomatous tissues that showed poor differentiation.
 Our results could show that HCMV might contribute to the development of the studied subsets of prostatic adenocarcinoma and benign prostatic hyperplasia.
Suha A. AL-Fakhar, Saad Hasan Mohammed Ali, Khalil Ismail A. Mohammed, Wifaq M. Ali, and Jinan M. Mousa
A and V Publications
Background: Diarrhea is a common problem in patients with hematologic disease and in patients with immuno compromising conditions. Objective: The aim of the study was to evaluate the presence risk factors that leading to acquire infection with Cl. difficile in children infected sever diarrhea in and immuno compromised patients. Material and Methods: The study was performed on freshly collected stool samples among 32 patients (13 females,19 males) with acute diarrheal in children their ages (1.5 months – 1.3 years) admitted to Pediatric Teaching Hospitals and adults admitted to Baghdad Teaching Hospital (patients with low cellular immunity like ALL, AML (20-75years) from April 2017 to April 2019. A questionnaire was completed for each patient name, age, gender, clinical symptoms like fever, diarrhea, constipation and, abdominal pain. The criteria included also, the risk factors that leading to suppression of cellular immunity. Fresh stool samples were tested by immunochromatographic assay for antigenic detection of Clostridium difficile Ag. Results: C. Idifficile antigen was identified only in three stool samples of male their mean ± S.D. was (24.75-24.37) from 32 patients (13 females,19 males) their ages rang (1.5months-75years), seven of them suffered blood malignancies (such as AML, ALL), while 21patients (seven of them babies) suffered from acute diarrhea with different causes (E. histolytica and fungal infections). All patients had fever, flatulence and abdominal patients, while 4 of patients with low cellular immunity. Conclusion: Cl. diffcile antigen present only few number of patients in three stool samples of maleswho suffered from sever diarrhea, or ALL (acute lymphocytic leukemia), or NHL (non-hodgkinse lymphoma).
Hind Hamed Shaker, Luma Amer Yasir, Ausama Abed-Alkadum Alajeely, Saad Hasan Mohammed Ali, and Shakir H. Mohammed Al-Alwany
Africa Health Research Organization
Suha A. AL-Fakhar, Wifaq M. Ali, Khitam Yahya Obaid, Khalil Ismail A Mohammed, Saad Hasan Mohammed Ali, and Jinan M. Mousa
Diva Enterprises Private Limited
Marwa Mohammed Ali, Majid Mohammed Mahmood, and Saad Hasan Mohammed Ali
Diva Enterprises Private Limited
Marwa Mohammed Ali Jassim, Majid Mohammed Mahmood, Saad Hasan Mohammed Ali, and Mohammed Sobhi Kamal
Diva Enterprises Private Limited
Hayat Ghaith Sachit, Taghreed F. Almahbobi, Zahraa Muhsen M. Ali, Saad Hasan Mohammed Ali, and Shakir H. Mohammed Al-Alwany
Journal of Pure and Applied Microbiology
Khalil Ismail A. Mohamed, Wifaq M. Ali Al-Wattar, Ghada Ibrahim Taha, Saad Hasan Mohammed Ali, Huda Q. Mohammed Abu-Al-Ess, Suha A. Al-Fukhar, and Jinan M. Mousa
Journal of Pure and Applied Microbiology
Saad H. Mohammed Ali, Taghreed F Almahbobi, Yasmeen J. AL-Bayaa, and Shakir H. Mohammed Al-Alwany
Diva Enterprises Private Limited
Muhi Kadhem Wannas Al-Janabi, Saad Hasan Mohammed Ali, Nadia Aziz Nasir, and Muhammed Wehby
Springer Science and Business Media LLC