Syazili Mustofa

@fk.unila.ac.id

Department of Biochemistry, Molecular Biology and Physology, Medical Faculty, Universitas Lampung
Universitas Lampung

Syazili Mustofa


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https://researchid.co/1lmuduni4

EDUCATION

MD, Doctor of Science

RESEARCH, TEACHING, or OTHER INTERESTS

Medicine, Biochemistry, Genetics and Molecular Biology, Pulmonary and Respiratory Medicine, Clinical Biochemistry
7

Scopus Publications

1163

Scholar Citations

18

Scholar h-index

40

Scholar i10-index

Scopus Publications

  • Inhibition of Growth MCF-7 Breast Cancer Cell with Umbilical Cord Mesencymal Stem Cells (UCMSCs) Derived Secretome
    Evi Kurniawaty, Syazili Mustofa, Silvia Andriani, Suharyani Suharyani S
    Current Trends in Biotechnology and Pharmacy, 2026
  • Limitations of blood culture, cardiovascular sequelae and the emerging role of metagenomic next generation sequencing in early-onset neonatal sepsis among premature infants
    Revista Latinoamericana De Hipertension, 2026
  • Black pepper (Piper nigrum L.) fruit extract ameliorates erectile dysfunction in alloxan-induced diabetic rats
    Exsa Hadibrata, Sutyarso Sutyarso, Hendri Busman, Syazili Mustofa, Wawan Abdullah Setiawan, et al.
    F1000research, 2026
    Background Diabetes mellitus (DM) is a chronic metabolic disorder frequently associated with male sexual and reproductive dysfunction, including erectile dysfunction (ED), reduced libido, and impaired spermatogenesis. Black pepper ( Piper nigrum L.) fruit extract has been reported to possess antidiabetic and reproductive benefits, yet its effect on DM-related ED remains underexplored. Objective To evaluate the effects of Piper nigrum fruit extract on erectile function, libido, sperm parameters, and testicular histology in alloxan-induced diabetic male rats. Methods Thirty male Sprague Dawley rats were divided into five groups: normal control, diabetic control, diabetic rats treated with black pepper extract (122.5 or 245 mg/kg BW), and diabetic rats treated with sildenafil citrate. Erectile function was assessed via penile reflexes, libido by mating behavior, sperm quality by concentration, motility, and morphology, and testicular histology by Leydig cell and spermatogonia counts. Data were analyzed using ANOVA with significance at p<0.05. Results Alloxan-induced diabetic rats showed significant impairment in erectile function, libido, sperm quality, and testicular histology (p<0.001 vs control). Black pepper extract at 122.5 mg/kg BW significantly improved total penile reflexes compared with diabetic controls (9.33±1.03 vs 6.00±1.26, p=0.02). Libido parameters including courtship latency (5.50±0.55 vs 21.00±9.47, p=0.013), mount latency (19.00±10.81 vs 37.17±6.31, p=0.009), and mount frequency (18.05±5.99 vs 7.17±1.83, p=0.002) were significantly improved. Sperm analysis revealed increases in sperm concentration (19.2±6.7 vs 12.6±1.3, though not significant, p=0.877), motility (31.8±23 vs 27±30, p=0.697), and normal morphology (40.9±7.8 vs 35±10.8, p=0.04). Testicular histology showed restoration of Leydig cell count (59.33±4.0 vs 30.50±3.86, p=0.035) and spermatogonia number (319.4±64.59 vs 491±37.0, p<0.001). The 245 mg/kg BW dose primarily improved sperm concentration (62.95±29.4 vs 12.6±1.3, p=0.01) and motility (36.6±23 vs 27±30, p=0.436). Sildenafil citrate significantly enhanced most parameters compared with diabetic controls (p<0.05). Conclusion Piper nigrum fruit extract ameliorates sexual dysfunction and reproductive impairment in alloxan-induced diabetic rats, particularly at 122.5 mg/kg BW, with significant improvements in erectile function, libido (p<0.05), sperm quality (p≤0.04), and testicular histology (p=0.035). These findings suggest its potential as a natural therapeutic agent for DM-related male reproductive dysfunction.
  • Effects of Black Pepper (Piper nigrum L.) Fruit Extract on Sexual Function, Hormonal Parameters, Sperm Parameters, Testicular Histology, and eNOS Expression in Alloxan-Induced Diabetic Rats
    Exsa Hadibrata, Sutyarso Sutyarso, Hendri Busman, Syazili Mustofa, Wawan Abdullah Setiawan, et al.
    F1000research, 2026
    Background Diabetes mellitus (DM) is often associated with male sexual and reproductive dysfunction, including erectile impairment, reduced libido, hormonal disturbances, and poor semen quality. Black pepper ( Piper nigrum L.) fruit extract has shown antidiabetic and reproductive benefits in experimental studies, but its role in diabetes-related sexual dysfunction remains unclear. Objective To evaluate the effects of black pepper fruit extract on hormonal, erectile, libido, sperm, histological, and molecular parameters in alloxan-induced diabetic male rats. Methods Thirty male Sprague Dawley rats were assigned to five groups: normal control, diabetic control, diabetic rats treated with black pepper extract at 122.5 or 245 mg/kg body weight (BW) for 8 consecutive days, and diabetic rats treated with sildenafil citrate 1 mg/kg BW. Outcomes included intratesticular testosterone (ITT), erectile parameters (quick flip, long flip, erection, total penile reflexes [TPR]), libido parameters (courtship latency [CL], mount latency [ML], mount frequency [MF]), sperm parameters (concentration, progressive motility, normal morphology), Leydig cell and spermatocyte counts, and eNOS mRNA expression. Data were analyzed using the Kruskal-Wallis test followed by Dunn’s post hoc test. Results Compared with diabetic controls, the 122.5 mg/kg BW black pepper group significantly improved TPR, CL, ML, and MF. The 245 mg/kg BW group significantly improved ITT, sperm concentration, and normal sperm morphology. Both black pepper doses increased Leydig cell counts. Sildenafil significantly improved TPR, sperm concentration, progressive motility, and normal morphology. eNOS mRNA expression was higher in all treatment groups than in diabetic controls. Conclusion Black pepper fruit extract showed dose-dependent effects in alloxan-induced diabetic rats. The lower dose was more beneficial for erectile and libido-related parameters, whereas the higher dose had greater effects on ITT and selected sperm outcomes. These findings support its potential as a preclinical candidate for diabetic male sexual and reproductive dysfunction.
  • Fruit Extract of Black Pepper (Piper nigrum L.) Ameliorate Male Reproductive Dysfunction in Alloxan-Induced Diabetic Rats
    Urology Journal, 2026
  • Influence of Rhizophora apiculata barks extract on Cholesterol, Triglyceride, LDL, and HDL Levels of Rattus norvegicus (Sprague Dawley) fed high-cholesterol diet
    Syazili Mustofa, Andi Nafisah Tendri Adjeng, Evi Kurniawaty, Lovina Ramadhita, Tania Tamara
    Research Journal of Pharmacy and Technology, 2024
    Background:Hypercholesterolemia is a lipid metabolic condition associated with cardiovascular disease (CVD), the leading cause of death worldwide. Rhizophora apiculata is a plant that grows along the Indonesian coast and may provide significant support for using traditional medicine. Bioactive molecules extracted from Rhizophora apiculta can be used in supplementary medicines and considered to be an alternative way to address the ever-increasing requirements of novel drugs. More research is needed to substantiate the usage of Rhizophora apiculate astraditional medicine.This study aims to investigate the effects of Rhizophora apiculata barks (RAB) ethanolic extract on lipid metabolism, namely Total Cholesterol (TC), Triglyceride (TG), LDL, and HDL levels in mice fed a high-cholesterol diet (HCD). Methods: The study comprised 30 white male rats (Rattus norvegicus) of the Sprague Dawley strain, aged 2-3 months and weighing 200-250 grams. Six groups of mice (n = 5) were formed. Group 1 was fed a conventional diet; Group 2 was given the High-Cholesterol Diet (HCD); Group 3 was given HCD and simvastatin 40 mg/kgbwt (p.o. ); and Groups 4, 5, and 6 were treated with HCD and RAB extract 56.55 mg/kgbwt (p.o.), RAB 28.28 mg/kgbwt (p.o.), and RAB 14.14 mg/kgbwt. All of the treatments began on the same day and lasted 30 days. Results: RAB-treated animal groups (56.55; 28.28; and 14.14 mg/kgbwt) had markedly lessened TC levels by 41.18%, 35.57%, and 9.24%, respectively; TG levels declined by 45.85%; 43.77%; and 27.74%, respectively; LDL levels reduced by 26.08%; 16.55%; and 11.21%, respectively; and HDL levels increased by 19.37%; 15.12%; and 13.21%, respectively. Conclusion: RAB-treated animal groups (56.55; 28.28; and 14.14 mg/kg) had markedly lessened total cholesterol, triglyceride, LDL and increased HDL levels (p &lt; 0.01) related to the HCD alone batch. These findings imply that the ethanolic extract of Rhizophora apiculata barks influences lipid metabolism and has potency as traditional medicine.
  • Decreased plasma epidermal growth factor (EGF) levels in patients with severe chronic obstructive pulmonary disease
    Retno AS Soemarwoto, Jamsari, Yanwirasti, Andika Chandra Putra, Syazili Mustofa
    Pneumologia, 2019
    Background Chronic mucus hypersecretion is a common feature in chronic obstructive pulmonary disease (COPD) and is associated with epidermal growth factor (EGF) activity. Aberrant EGF and its receptor signalling can cause airway hyperproliferation, increase in mucous cell differentiation and mucus hyperproduction. Furthermore, it can also promote subepithelial fibrosis and excessive collagen deposition in COPD. The objective of this research was to investigate the plasma levels of EGF in smokers with COPD in comparison with clinically healthy smokers. In addition, the relationship between the plasma levels of EGF and clinical features was investigated. Methods A cross-sectional study included 82 clinically stable male patients with mild-to-very severe COPD (mean age: 64.5±8.6 years), and the control group consisted of 86 healthy male smokers (mean age: 61.6±9.5 years). To define COPD, we performed spirometry and classified COPD using Global Initiative for Chronic Obstructive Lung Disease (GOLD) classification. We analyzed the levels of EGF by enzyme-linked immunosorbent assay in plasma. Results The mean serum levels of EGF were significantly lower in smokers with COPD than those in controls (69.30 and 83.82 pg/mL, respectively, p = 0.046). The plasma levels of EGF were significantly different (p = 0.004) between mild COPD and moderate-to-very severe COPD. There were no significant differences between the levels of EGF in plasma of spontaneous sputum producers (COPD patients) vs. nonsputum producers (p = 0.101) and between nonexacerbated COPD and exacerbated COPD patients(p = 0.138). Conclusions There is a significant difference in the plasma levels of EGF in male smokers with COPD as compared with male healthy smokers. Our findings suggest that the plasma levels of EGF may contribute to the pathogenesis of COPD.

RECENT SCHOLAR PUBLICATIONS

  • Design and Molecular Validation of eNOS PCR Primers for Gene Expression Studies in Rat Corpus Cavernosum: A Tool for Erectile Dysfunction Research
    E Hadibrata, WA Setiawan, AN Putri, S Sutyarso, H Busman, S Mustofa, ...
    F1000Research 15, 643 , 2026
    2026
  • Complex Case Of Copd Exacerbation With Lung Cancer And Paraneoplastic Syndrome: A Case Report
    S Mustofa, FZ Sidik, E Kurniawaty, M Rohman, N Karima
    Jurnal Ilmu Kedokteran dan Kesehatan 13 (4), 861-867 , 2026
    2026
  • Case Report: Complex Management of COPD Exacerbation, Lung Cancer, and Paraneoplastic Syndrome
    S Mustofa, FZ Sidik, E Kurniawaty, M Rohman, N Karima
    Jurnal Ilmu Kedokteran dan Kesehatan 13 (4), 861-867 , 2026
    2026
  • Keberhasilan Pleurodesis Bleomisin Pada Pneumotoraks Spontan Sekunder Berulang Akibat Tuberkulosis Paru: Laporan Kasus
    S Mustofa, RA Fitri, N Choerunnisa, RA Soemarwoto, GT Putri, H Busman
    Jurnal Ilmu Kedokteran dan Kesehatan 13 (3), 685-690 , 2026
    2026
  • Acetazolamide-mediated carbonic anhydrase inhibition suppresses human peripheral blood mononuclear cell proliferation via G1/S cell cycle arrest
    S Mustofa, M Sadikin
    Acta Biochimica Indonesiana 9 (1), 145-145 , 2026
    2026
  • Peran Eksosom dalam Regenerative Medicine
    S Mustofa, ZN Z
    2026
  • Sindrom Metabolik: Konsep, Biomarker dan Inovasi Diagnostik
    S Mustofa, SO Handayani
    2026
  • Luka Bakar: Epidemiologi, Patofisologi Dan Faktor Faktor Yang Mempercepat Penyembuhannya
    MDA Arja, S Mustofa
    Medical Profession Journal of Lampung 16 (3) , 2026
    2026
  • Laporan Kasus: Penatalaksanaan Asma Berat Pada Pasien Dengan Pneumonia Dan Diabetes Mellitus
    S Mustofa, MK Dewi, GT Putri, E Kurniawaty, FRH Sirait, RA Soemarwoto
    Jurnal Ilmu Kedokteran dan Kesehatan 13 (1), 89-97 , 2026
    2026
  • Comparison of Conventional and Modern Extraction Methods on the Antibacterial Activity of Natural Products
    ARP Ratu, S Mustofa, GT Putri, H Busman
    Medical Profession Journal of Lampung 16 (3), 76-85 , 2026
    2026
  • The Efektivitas Sediaan Topikal Berbasis Tanaman Herbal terhadap Penyembuhan Luka Sayat pada Studi In Vivo: Sebuah Tinjauan Naratif: Sebuah Tinjauan Studi Hewan Coba
    RR Hakim, S Mustofa, GT Putri, H Busman
    Medical Profession Journal of Lampung 16 (3), 86-92 , 2026
    2026
  • Black pepper (Piper nigrum L.) fruit extract ameliorates erectile dysfunction in alloxan-induced diabetic rats
    E Hadibrata, S Sutyarso, H Busman, S Mustofa, WA Setiawan, RDP Sari, ...
    F1000Research 15, 38 , 2026
    2026
  • Inhibition of Growth MCF-7 Breast Cancer Cell with Umbilical Cord Mesencymal Stem Cells (UCMSCs) Derived Secretome.
    E Kurniawaty, S Mustofa, S Andriani
    Current Trends in Biotechnology & Pharmacy 20 (1), 2822 , 2026
    2026
  • PENATALAKSANAAN ASMA BERAT PADA PASIEN DENGAN PNEUMONIA DAN DIABETES MELLITUS: LAPORAN KASUS
    S Mustofa, MK Dewi, GT Putri, E Kurniawaty, FRH Sirait, RA Soemarwoto
    Jurnal Ilmu Kedokteran dan Kesehatan 13 (1) , 2026
    2026
  • Identification of Active Compound and Effectiveness Test of 96% Tithonia diversifolia Ethanol Extract on Mortality of Aedes aegypti Mosquito
    AP Gadila, S Mustofa, R Graharti, E Setyaningrum
    Medical Profession Journal of Lampung 16 (1), 155-162 , 2026
    2026
  • Uji Toksisitas Subkronik Ekstrak Kulit Batang Bakau (Rhizophora Apiculata) Terhadap Kerusakan Ginjal Tikus Putih Jantan (Rattus Norvegicus) Galur Sparague Dawley
    RY Maulana, S Mustofa, H Busman, E Kurniawaty
    Medical Profession Journal of Lampung 16 (1), 110-118 , 2026
    2026
  • Peran Penyuluhan Kesehatan dalam Pencegahan Penyakit Menular Pascabencana Banjir di Kabupaten Aceh Tamiang
    RZ Oktarlina, E Kurniawaty, S Mustofa, TA Happy, R Pratiwi, DF Pradata, ...
    Jurnal Pengabdian Dharma Wacana 6 (3), 465-471 , 2025
    2025
  • Strategi Penguatan Kesehatan Mental dan Psikososial Pasca-Banjir Aceh Tamiang 2025
    RZ Oktarlina, E Kurniawaty, S Mustofa, TA Happy, R Pratiwi, DF Pradata, ...
    Jurnal Pengabdian Dharma Wacana 6 (3), 458-464 , 2025
    2025
  • Indonesian Medicinal Plants for Anticancer
    S Mustofa
    Journal of Pharmaceutical and Natural Resources 1 (2), 1-10 , 2025
    2025
  • Indonesian Marine Natural Product for Anticancer
    FZ Sidik, S Mustofa, E Kurniawaty, M Rohman
    Jurnal Agromedicine Unila: Jurnal Kesehatan dan Agromedicine 12 (2), 107-115 , 2025
    2025

MOST CITED SCHOLAR PUBLICATIONS

  • Hipertensi: Gambaran Umum
    NR Aditya, S Mustofa
    stroke 5 (9), 10 , 2023
    2023
    Citations: 59
  • Pengaruh pemberian ekstrak kulit batang bakau minyak (Rhizophora apiculata) etanol 95% terhadap arteri koronaria tikus putih (Rattus novergicus) jantan galur Sprague Dawley …
    S Mustofa, N Alfa, AJ Wulan, S Rahmanisa
    Jurnal Kedokteran Universitas Lampung 3 (1), 28-33 , 2019
    2019
    Citations: 48
  • The effect of Mangrove (Rhizophora Apiculata) bark extract ethanol on histopathology pancreas of male white rats Sprague Dawley strain exposed to cigarette smoke
    S Mustofa, E Kurniawaty, S Rahmanisa
    Acta Biochimica Indonesiana 1 (1), 7-13 , 2018
    2018
    Citations: 48
  • Pengaruh Ekstrak Etanol Daun Rhizophora apiculata terhadap Kolesterol Total dan Trigliserida Rattus norvegicus Galur Sprague dawley yang Diinduksi Diet Tinggi Lemak
    S Mustofa, DW Sumekar, H Busman
    Jurnal Kesehatan 13 (3), 472-477 , 2022
    2022
    Citations: 42
  • The protective effect of Rhizophora apiculata bark extract against testicular damage induced by cigarette smoke in male rats
    S Mustofa, F Hanif
    Acta biochimica indonesiana 2 (1), 23-31 , 2019
    2019
    Citations: 40
  • Ekspresi IL-6 dan TNF-α pada obesitas
    T Susantiningsih, S Mustofa
    Jurnal Kedokteran Universitas Lampung 2 (2), 174-180 , 2018
    2018
    Citations: 40
  • Manajemen gangguan saluran cerna: Panduan Bagi dokter umum
    S Mustofa, E Kurniawaty
    Bandar Lampung: Aura Printing & Publishing. hlm 272 , 2013
    2013
    Citations: 39
  • Efek hepatoprotektif ekstrak etanol rhizophora apiculata pada tikus yang dipaparkan asap rokok
    S Mustofa, V Anisya
    Jurnal Kedokteran Universitas Lampung 4 (1), 12-17 , 2020
    2020
    Citations: 34
  • Efek Protektive Kardiovaskular Ekstrak Rhizophora Apiculata Berbagai Pelarut pada Tikus Yang Dipaparkan Asap Rokok
    S Mustofa, ZY Fahmi
    Jurnal Kedokteran Universitas Lampung 5 (1), 7-15 , 2021
    2021
    Citations: 33
  • Subacute toxicity test of Rhizophora apiculata bark extract on liver and pancreas histopathology of rats
    S Mustofa, I Ciptaningrum, CS Zuya
    Acta Biochimica Indonesiana 3 (2), 89-97 , 2020
    2020
    Citations: 29
  • The influence of Piper retrofractum Vahl (Java's chili) extract towards lipid profile and histology of rats coronary artery with high-fat diet
    M Maulana
    Juke Unila , 2014
    2014
    Citations: 26
  • Pengaruh Pemberian Ekstrak Etanol 95% Kulit Batang Bakau Minyak (Rhizophora apiculata) terhadap Kadar MDA Tikus Putih (Rattus norvegicus) Galur Sprague dawley yang Dipaparkan …
    B Caesario, S Mustofa
    MEDULA, medicalprofession journal of lampung university 9 (1), 43-47 , 2019
    2019
    Citations: 23
  • Influence of Rhizophora apiculata barks extract on Cholesterol, Triglyceride, LDL, and HDL Levels of Rattus norvegicus (Sprague Dawley) fed high-cholesterol diet
    S Mustofa, ANT Adjeng, E Kurniawaty, L Ramadhita, T Tamara
    Research Journal of Pharmacy and Technology 17 (1), 396-400 , 2024
    2024
    Citations: 22
  • Efek protektif ekstrak kulit batang bakau Rhizophora apiculata terhadap kerusakan histologi paru Rattus norvegicus yang diinduksi asap rokok
    S Mustofa, CY Tarigan
    Jurnal Kesehatan 14 (2), 241-250 , 2023
    2023
    Citations: 22
  • Likopen, antioksidan eksogen yang bermanfaat bagi fertilitas laki-laki
    N Alfa, S Mustofa, NAV Irawati
    Jurnal Majority 8 (1), 237-241 , 2019
    2019
    Citations: 21
  • Diagnosis dan Penatalaksanaan Karsinoma Mammae Stadium 2
    R Humaera, S Mustofa, F Kedokteran
    J Medula Unila 7 (2), 103-7 , 2017
    2017
    Citations: 20
  • Identifikasi Telur Soil Transmitted Heminths pada Lalapan Kubis (Brassic oleracea) Di Warung-Warung Makan Universitas Lampung
    KP Wardhana, B Kurniawan, S Mustofa
    Medical Jurnal Of Lampung University, 87-88 , 2014
    2014
    Citations: 20
  • Potensi Rhizophora apiculata Sebagai Fitofarmaka
    MA Wardina, S Mustofa, ANTA Malarangeng
    Medical Profession Journal of Lampung 13 (2), 137-146 , 2023
    2023
    Citations: 19
  • Diagnosis dan tatalaksana kolelitiasis
    AR Adhata, S Mustofa, TU Soleha
    Medical Profession Journal of Lampung 12 (1), 75-78 , 2022
    2022
    Citations: 18
  • Drug-Induced Liver Injury Akibat Penyalahgunaan Parasetamol
    A Anindyaguna, S Mustofa, DI Anggraini, RZ Oktarlina
    Medical Profession Journal of Lampung 12 (3), 500-507 , 2023
    2023
    Citations: 16