Niraj Tripathi
@jnkvv.org
Directorate of Research Services
Jawaharlal Nehru Krishi Vishwa Vidyalaya
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, General Agricultural and Biological Sciences, Plant Science, Biotechnology
Scopus Publications
Scopus Publications
Rahul Sharde, Manoj Kumar Tripathi, Deepa Bhatt, Sushma Tiwari, Mohini Sharma, Yashi Singh Tomar, and Niraj Tripathi
Springer Science and Business Media LLC
Nishi Mishra, M.K. Tripathi, Sushma Tiwari, Niraj Tripathi, Neha Gupta, and Akash Sharma
Agricultural Research Communication Center
Background: Soybean is a key crop that grants an imperative supply of oils and proteins to humans and animals; however, its productivity spectacularly diminished owing to the occurrence of drought stress. Methods: The present investigation was executed during Kharif 2018-2019 to recognize drought tolerant genotypes on the basis of an array of morpho-physiological traits. Morpho-physiological analysis among 53 genotypes divulged the existence of drought tolerance capability in studied genotypes.Result: On the basis of current findings, it can be concluded that drought stress retards the growth and metabolic activity of soybean genotypes. These parameters showed considerable amount of variability under drought stress at different growth stages in soybean. Among 53 soybean genotypes, four genotypes viz., JS97-52, AMS 2014-1, RVS-14 and NRC-147 was found to be drought tolerant.
Prerana Parihar, R.K Pandya, Purnima Singh, Sushma Tiwari, M.K Tripath, Niraj Tripathi, and C Tara Satyavathi
Range Management Society of India
Pearl millet is an important nutri-cereal for humans and a forage crop for livestock. It is ()Pennisetum glaucumthe only cereal crop that is proficient in adopting punitive climate conditions and peripheral soil. The blast disease caused by is a major problem in pearl millet, causing significant losses in high-yield Pyricularia griseahybrids/varieties grown for fodder. Blast disease is a major biotic threat in the cultivation of pearl millet in northern Madhya Pradesh, especially Gwalior, Morena, Bhind and Sheopur. In this investigation, we studied eight isolates of that cause pearl millet blast disease. We used RAPD and ISSR molecular markers to P. griseaassess their morphology, pathogenicity and genetics. Mycelium growth was investigated maximum in isolate PGMP1, howeverThe pearl millet blast , two isolates ., PGMP1 and PGMP8 were found to be highly virulent. vizfungus population in northern Madhya Pradesh is genetically diverse. RAPD and ISSR markers reliablyexplained the relationships among the different isolates. Our results on the characterization of blast pathogen might be advantageous in devising location-specific disease management strategies in pearl millet against blast disease.
Moni Thomas, Niraj Tripathi, Shibu M. Eappen, Kailash C. Meena, Atul Shrivastava, and Niranjan Prasad
Springer Science and Business Media LLC
AbstractGuggul is a gum oleo-resin, tapped from a data deficient plant- Commiphora wightii (Arnott.) Bhandari in India. It is extensively used in ayurvedic drugs and formulations since ages. Natural plant-based products; especially aromatic ones like guggul gum oleo-resin deteriorates, qualitatively during its storage and transits before reaching the industry for its value addition. This economical and ecological loss can be avoided if it is stored in proper containers. Physico-chemical degradation of guggul samples stored were analysed by scanned electron microscopy, fourier transformed infra red, thermogravimatric, Powdered X-ray diffraction and elemental analysis for carbon, hydrogen, nitrogen and sulphur. Physico-chemical degradation of guggul oleo-resin occurs with the age of storage and the type of storage containers used. Among the four storage containers (earthen pot, plastic jar, polythene bag, jute bag) evaluated, earthen pot was found to be the best in checking the qualitative loss of guggul even upto 24 months. The qualitative information generated in the study on guggul storage may be useful to the drug industry and guggul traders. It may encourage them practice storing guggul in earthen pots against current practice of using jute bags and polythene bags, to store it.
Prakash N. Tiwari, Sharad Tiwari, Swapnil Sapre, Anita Babbar, Niraj Tripathi, Sushma Tiwari, and Manoj Kumar Tripathi
Agricultural Research Communication Center
Background: Being a vital source of high-quality dietary protein, chickpea is an unavoidable legume. The present investigation was performed to study the applicability of the microsatellite markers linked with drought tolerance in Indian chickpea genotypes collected from different genetic background. Methods: In Rabi 2021-22, forty chickpea genotypes including national check for drought tolerance, elite cultivars, released varieties and advanced breeding lines were screened employing forty microsatellite markers linked with drought tolerance associated traits. Result: Among forty drought tolerance related microsatellite markers, twenty-six were found to be polymorphic and produced a total of 66 alleles, with a mean of 2.5 alleles per locus. Model-based population structure analysis clearly distinguished the drought tolerant genotypes including ICC4958, JG74, JAKI9218, JG16, JG6, JG14 and JG11. The principle coordinate analysis (PCoA) and analysis of molecular variance (AMOVA) further confirmed these results. Findings of the present investigation would have a greater potential for further utilization in breeding of drought specific chickpea cultivar(s).
Prakash N. Tiwari, Sharad Tiwari, Swapnil Sapre, Niraj Tripathi, Devendra K. Payasi, Mrinalini Singh, Satyendra Thakur, Mohini Sharma, Sushma Tiwari, and Manoj Kumar Tripathi
MDPI AG
Chickpea is widely grown in rainfed areas of developing countries because of its nutritional abundance and adaptability. To overcome the environmental effect of drought on yield, a characteristic-linked selection strategy is proved as well-thought-out and advantageous for the development of drought-tolerant cultivars. To precisely understand the contribution of various physio-biochemical and yield-attributing traits toward drought tolerance in chickpea (Cicer arietinum L.), forty chickpea genotypes were evaluated in the years 2020–2021 and 2021–2022 under normal irrigated as well as drought-stressed conditions. Among the studied genotypes, genotype ICC4958 retained the highest chl content (0.55 mg g−1 FW), minimal electrolyte leakage, and superoxide dismutase (1.48 U/mg FW) and peroxidase (2.21 µmol/min/g FW) activities while cultivar JG11 maintained the maximum relative water content and proline accumulation. The principal-component-based biplots prioritized the physio-biochemical and yield-accrediting characteristics based on their association significance and contribution to terminal drought tolerance. Under drought stress, grain yield per plant was depicted to have a strongly positive association with canopy temperature depression, catalase, superoxide dismutase, and peroxidase activities as well as total soluble sugar, proline, and chlorophyll content, along with the numbers of pods and biological yield per plant. These identified physio-biochemical and yield-attributing traits can be further deployed to select drought-tolerant chickpea genotypes for the breeding of climate-smart chickpea genotypes.
Shivani Singh Rana, Sushma Tiwari, Neha Gupta, Manoj Kumar Tripathi, Niraj Tripathi, Sangeeta Singh, and Sameer S. Bhagyawant
MDPI AG
Millets are group of underutilized cereal crops with higher nutritional values. The present investigation used different classes of minor millets, including barnyard (sava), little (kutki), finger (ragi), kodo and foxtail millets, for evaluation of their nutritional parameters, i.e., the content of proteins, total amino acids, total sugars, insoluble fibers, soluble fibers, total dietary fibers, iron (Fe) and zinc (Zn), along with antinutritional and antioxidant parameters, viz., tannic acid, phytic acid, phenol, flavonoid, proline and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Alpha amylase and alpha glucosidase activity were also thought to elevate millets as a viable staple meal. Foxtail millet showed the maximum inhibition, with an IC50 value of 20.46 ± 1.80 µg mL−1 with respect to α-amylase. The coefficient of correlation between nutritional and antinutritional compositions showed that the starch content was significantly and positively correlated with insoluble fiber (r = 0.465) and dietary fiber (r = 0.487). Moreover, sugar was positively correlated with the phytic acid (r = 0.707), Fe and Zn (r = 0.681) contents. To determine the peptides responsible for anticancer activity, the foxtail protein was subjected to ultrafiltration; it was found that the 3 kDa fraction retained the greatest anticancer activity. Selected millet germplasm line(s) that have the best nutraceutical properties could be used in millet improvement programs.
Shraddha Tomar, Stuti Sharma, Niraj Tripathi, Sunny Thakur, Nidhi Pathak, Radhyeshyam Sharma, and Priya Tiwari
Agricultural Research Communication Center
Background: Lentil is an important legume crop that plays a vital role in sustainable agriculture and human health. However, the restricted genetic foundation or parentage of contemporary cultivars has arisen as a serious challenge for lentil development. Therefore, determining the genetic diversity and yield-related characteristics is crucial for the breeder to broaden the genetic base and aid in selecting the desirable parents for hybrid development programmes. Methods: In the current study, microsatellite markers were used for diversity analysis among 37 lentil genotypes. Morphological and molecular systems both were able to differentiate lentil genotypes. Among applied SSR primers 10 were able to produce successful amplifications with template DNA of all the studied genotypes of lentil. Result: A sum of 357 scoreable bands were produced, 148 of which accounted for 41.45% polymorphism. The UPGMA dendrogram grouped 37 lentil genotypes into 2 groups. PIC values ranged from 0.37 to 0.77. For this experiment, SSR 80, SSR 130, SSR 34-2 and SSR 33 were highly informative polymorphic markers, demonstrating the efficacy and higher resolution in exposing molecular genetic diversity among lentil genotypes. The highest numbers of alleles (5) were produced by SSR 80 primer which was selected as extremely polymorphic. This study reveals the variation across lentil genotypes, which might be employed further in breeding efforts for lentils that result in strong heterosis in the segregating generation. The SSR markers found as polymorphic may also be utilized for further polymorphism analysis among different set of lentil genotypes.
Rakesh Kumar Yadav, Manoj Kumar Tripathi, Sushma Tiwari, Niraj Tripathi, Ruchi Asati, Shailja Chauhan, Prakash Narayan Tiwari, and Devendra K. Payasi
MDPI AG
Genome editing aims to revolutionise plant breeding and could assist in safeguarding the global food supply. The inclusion of a 12–40 bp recognition site makes mega nucleases the first tools utilized for genome editing and first generation gene-editing tools. Zinc finger nucleases (ZFNs) are the second gene-editing technique, and because they create double-stranded breaks, they are more dependable and effective. ZFNs were the original designed nuclease-based approach of genome editing. The Cys2-His2 zinc finger domain’s discovery made this technique possible. Clustered regularly interspaced short palindromic repeats (CRISPR) are utilized to improve genetics, boost biomass production, increase nutrient usage efficiency, and develop disease resistance. Plant genomes can be effectively modified using genome-editing technologies to enhance characteristics without introducing foreign DNA into the genome. Next-generation plant breeding will soon be defined by these exact breeding methods. There is abroad promise that genome-edited crops will be essential in the years to come for improving the sustainability and climate-change resilience of food systems. This method also has great potential for enhancing crops’ resistance to various abiotic stressors. In this review paper, we summarize the most recent findings about the mechanism of abiotic stress response in crop plants and the use of the CRISPR/Cas mediated gene-editing systems to improve tolerance to stresses including drought, salinity, cold, heat, and heavy metals.
Shruti Paliwal, Manoj Tripathi, Sushma Tiwari, Niraj Tripathi, Devendra Payasi, Prakash Tiwari, Kirti Singh, Rakesh Yadav, Ruchi Asati, and Shailja Chauhan
MDPI AG
Flax, or linseed, is considered a “superfood”, which means that it is a food with diverse health benefits and potentially useful bioactive ingredients. It is a multi-purpose crop that is prized for its seed oil, fibre, nutraceutical, and probiotic qualities. It is suited to various habitats and agro-ecological conditions. Numerous abiotic and biotic stressors that can either have a direct or indirect impact on plant health are experienced by flax plants as a result of changing environmental circumstances. Research on the impact of various stresses and their possible ameliorators is prompted by such expectations. By inducing the loss of specific alleles and using a limited number of selected varieties, modern breeding techniques have decreased the overall genetic variability required for climate-smart agriculture. However, gene banks have well-managed collectionns of landraces, wild linseed accessions, and auxiliary Linum species that serve as an important source of novel alleles. In the past, flax-breeding techniques were prioritised, preserving high yield with other essential traits. Applications of molecular markers in modern breeding have made it easy to identify quantitative trait loci (QTLs) for various agronomic characteristics. The genetic diversity of linseed species and the evaluation of their tolerance to abiotic stresses, including drought, salinity, heavy metal tolerance, and temperature, as well as resistance to biotic stress factors, viz., rust, wilt, powdery mildew, and alternaria blight, despite addressing various morphotypes and the value of linseed as a supplement, are the primary topics of this review.
Prakash N. Tiwari, Sharad Tiwari, Swapnil Sapre, Anita Babbar, Niraj Tripathi, Sushma Tiwari, and Manoj Kumar Tripathi
MDPI AG
Chickpea production is seriously hampered by drought stress, which could be a great threat in the future for food security in developing countries. The present investigation aimed to screen the drought-tolerant response of forty desi chickpea genotypes against drought stress through various physio-biochemical selection indices and yield-attributing traits. Principle component-based biplot analysis recognized PG205, JG2016-44, JG63, and JG24 as tolerant genotypes based on physiological selection indices. These genotypes retained higher relative water content, stomatal conductance, internal CO2 concentration, and photosynthetic rate. ICC4958, JG11, JAKI9218, JG16, JG63, and PG205 were selected as tolerant genotypes based on biochemical selection indices. These genotypes sustained higher chlorophyll, sugar and proline content with enhanced antioxidant enzyme activities. With respect to yield trials, JAKI9218, JG11, JG16, and ICC4958 had higher seed yield per plant, numbers of pods, and biological yield per plant. Finally, JG11, JAKI9218, ICC4958, JG16, JG63, and PG205 were selected as tolerant genotypes based on cumulative physio-biochemical selection indices and yield response. These identified drought-tolerant genotypes may be further employed in climate-smart chickpea breeding programs for sustainable production under a changing climate scenario.
Satish Kachare, Sharad Tiwari, and Niraj Tripathi
Springer Science and Business Media LLC
Shikha Upadhyay, A.K. Singh, M.K. Tripathi, Sushma Tiwari, Niraj Tripathi, and R.P. Patel
Agricultural Research Communication Center
Background: Soybean is an important oilseed crop. Charcoal rot caused by soil borne polyphagous fungus Macrophomina phaseolina in soybean causes about 80 percent yield loss. Methods: Callus and cell suspension cultures derived from immature and mature embryonic axis and cotyledons explants from disease susceptible soybean cultivars (cv JS335 and JS95-60) were treated with purified toxic culture filtrate generated by the fungus Macrophomina phaseolina fortified with MS culture medium. A continuous method of four cycles of selection was executed on toxic medium while during discontinuous method, a silence was given after the second and third cycle of selection using non-toxic medium. Result: The discontinuous method appeared to be superior as it permitted the calli to recover their regeneration capability. Continuous exposure to toxic culture filtrate resulted up to about 65-75 percent mortality. A total of four lines of JS335 and nine of JS95-60 were found resistant amongst an array of putative resistant/tolerant lines during S1 generation.
Rakesh Kumar Yadav, Manoj Kumar Tripathi, Sushma Tiwari, Niraj Tripathi, Ruchi Asati, Vinod Patel, R. S. Sikarwar, and Devendra K. Payasi
MDPI AG
Chickpea is an important leguminous crop with potential to provide dietary proteins to both humans and animals. It also ameliorates soil nitrogen through biological nitrogen fixation. The crop is affected by an array of biotic and abiotic factors. Among different biotic stresses, a major fungal disease called Fusarium wilt, caused by Fusarium oxysporum f. sp. ciceris (FOC), is responsible for low productivity in chickpea. To date, eight pathogenic races of FOC (race 0, 1A, and 1B/C, 2-6) have been reported worldwide. The development of resistant cultivars using different conventional breeding methods is very time consuming and depends upon the environment. Modern technologies can improve conventional methods to solve these major constraints. Understanding the molecular response of chickpea to Fusarium wilt can help to provide effective management strategies. The identification of molecular markers closely linked to genes/QTLs has provided great potential for chickpea improvement programs. Moreover, omics approaches, including transcriptomics, metabolomics, and proteomics give scientists a vast viewpoint of functional genomics. In this review, we will discuss the integration of all available strategies and provide comprehensive knowledge about chickpea plant defense against Fusarium wilt.
Purnima Singh, Sushma Tiwari, Prerana Parihar, Reeti Singh, Niraj Tripathi, R.K. Pandya, and M.K. Tripathi
Agricultural Research Communication Center
Background: The prevailing methods for the genomic DNA extraction of Sclerotium rolfsii from its mycelium mat is often time consuming and yields poor quality and quantity of genomic DNA owing to presence of the outrageous magnitude of mucilage and polysaccharides. Methods: A new fast track method for DNA isolation from the resting structure (sclerotia) of S. rolfsii by modifying CTAB method deprived of supplementation of proteinase K has been standardized. Result: The protocol produced 500ng DNA from sclerotia with purity vacillating from 1.7 to 1.9 as confirmed by A260/A280 and A260/A230 spectrophotometric documentation. The DNA extracted from sclerotia commissioning protocol was efficaciously used for the further downstream reactions/process like PCR-RAPD, PCR-ISSR and ITS amplification of rDNA-ITS region.
Rahul Patidar, Shivam Vajpayee, Sumit Kakade, Moni Thomas, Niraj Tripathi, Anubha Upadhyay, Brajesh Kumar Namdev, Anil Kurmi, H.L. Sharma, and P.S. Kulhare
Agricultural Research Communication Center
Background: Pigeonpea [Cajanus cajan (L.) Millsp.] is an important deep-rooted pulse crop, predominantly cultivated in rainfed areas by both Indian and South African countries by small and marginal farmers. Incidentally, this group of farmers are malnourished and socio-economically backward.Methods: A low input technology-‘Jawahar model for doubling income of resource constrained marginal farmers’ was evaluated once again with different combination of soil microbes in a substrate filled in used Polypropylene bags.Conclusion: Total C. cajan seed yield per plant in 3 hand pickings varied from 1066.66g to 1254.83g in different treatment combination of soil microbes in a substrate. Lac- the next produce, from the plant varied from a mean of 327.47g to 386.07g per C. cajan plant. Two premium crops from the same plant with same effort and resources per unit area may help C. cajan growers to double their income, improve their household nourishment as well as soil nutrient status.
Ruchi Asati, Manoj Kumar Tripathi, Sushma Tiwari, Rakesh Kumar Yadav, and Niraj Tripathi
Cicer arietinum L. is the third greatest widely planted imperative pulse crop worldwide, and it belongs to the Leguminosae family. Drought is the utmost common abiotic factor on plants, distressing their water status and limiting their growth and development. Chickpea genotypes have the natural ability to fight drought stress using certain strategies viz., escape, avoidance and tolerance. Assorted breeding methods, including hybridization, mutation, and marker-aided breeding, genome sequencing along with omics approaches, could be used to improve the chickpea germplasm lines(s) against drought stress. Root features, for instance depth and root biomass, have been recognized as the greatest beneficial morphological factors for managing terminal drought tolerance in the chickpea. Marker-aided selection, for example, is a genomics-assisted breeding (GAB) strategy that can considerably increase crop breeding accuracy and competence. These breeding technologies, notably marker-assisted breeding, omics, and plant physiology knowledge, underlined the importance of chickpea breeding and can be used in future crop improvement programmes to generate drought-tolerant cultivars(s).
Nishi Mishra, M. K. Tripathi, Sushma Tiwari, Niraj Tripathi, and H. K. Trivedi
Agricultural Research Communication Center
Background: The growth and productivity of soybean are adversely affected by an array of biotic factors. Viruses are one of them as they cause great loss to the yield of soybean in India. The present study was conducted with an objective to identify yellow mosaic virus (YMV) resistant genotypes among the selected set of 53 soybean genotypes. Methods: The field screening was performed to identify YMV resistant genotypes. The field data was compared with molecular data recorded on the basis of gene specific SSR molecular markers. Result: During field study, 11 genotypes were found to be highly resistant, 26 resistant, 6 moderately resistant, 4 moderately susceptible, 3 susceptible, while three genotypes namely: JS335, JS 97-52 and RVS 2001-4 were found to be highly susceptible. In molecular analysis three genotypes viz.,: JS 20-29, JS 20-69 and JS 20-98 were found to be resistant against YMV. Among the polymorphic SSR markers the highest genetic diversity (0.4785) was observed with Satt554 while lowest genetic diversity (0.037) was observed with Satt308. Similarly polymorphism information content (PIC) was highest (0.364) in Satt554 and lowest (0.0363) in Satt308 among all polymorphic markers used for screening against YMV. The resistant genotypes identified in this study may be used as donor of resistance gene against YMV to develop improved genotypes which would stand as barrier against spread of the disease to newer areas and thus it can boost production and productivity of soybean in the country.
Ravindra Singh Solanki, Anita Babbar, and Niraj Tripathi
Bangladesh Journals Online (JOL)
Chickpea (Cicer arietinum L.) provides high quality protein for human and animal consumption as well as offers economic benefits to farmers because of the high market value for its grains. Fifty indigenous and exotic kabuli chickpea lines varying in seed yield and plant type were subjected to 20 SSR markers analysis for assessment of genetic diversity. Among twenty SSR primers, eleven of them were able to produce polymorphic bands. Quantitative traits based clustering pattern was found to be similar to molecular clustering for most of the kabuli genotypes such as JGK 1, JGK 2, JGK 3, JGK 32-1 and some of the ICRISAT (ICCVs) and ICARDA (FLIPs) lines. This indicated the association of used molecular markers with targeted quantitative traits in the present study. Bangladesh J. Bot. 51(3): 581-587, 2022 (September)
Niraj Tripathi, Manoj Kumar Tripathi, Sushma Tiwari, and Devendra K. Payasi
MDPI AG
Soybean (Glycine max (L.) Merr.) is an important leguminous crop and biotic stresses are a global concern for soybean growers. In recent decades, significant development has been carried outtowards identification of the diseases caused by pathogens, sources of resistance and determination of loci conferring resistance to different diseases on linkage maps of soybean. Host-plant resistance is generally accepted as the bestsolution because of its role in the management of environmental and economic conditions of farmers owing to low input in terms of chemicals. The main objectives of soybean crop improvement are based on the identification of sources of resistance or tolerance against various biotic as well as abiotic stresses and utilization of these sources for further hybridization and transgenic processes for development of new cultivars for stress management. The focus of the present review is to summarize genetic aspects of various diseases caused by pathogens in soybean and molecular breeding research work conducted to date.
Sushmita Mandloi, M.K. Tripathi, Sushma Tiwari, and Niraj Tripathi
Brill
Abstract Groundnut is an important oilseed crops with high nutritional values. Yield of groundnut is constrained owing to diverse biotic and abiotic factors. Amongst biotic stresses two foremost foliar fungal diseases viz., late leaf spot and rust are universal and sparingly significant. Both conventional and modern breeding methods may be applied for improvement of groundnut. The current investigation was carried out to recognize genetic variability present among different groundnut germplasm with the use of morphological characters and SSR markers with the screening of resistant to leaf spot and rust resistant genotypes. In morphological examination, the analysis of variance divulged highly substantial differences among 48 germplasm lines. Initial screening of Germplasm was tried with the use of 125 SSR markers. But, based on banding pattern, 21 markers were found to be the best and selected for amplification of whole set of genotypes. Out of 21 markers, 16 were found to be highly polymorphic. Highest PIC value (0.8196) was detected for marker S021 representing 81% diversity. SSR Markers viz., PM42, PM204, PM377, S052, S076, S078 and S078 have PIC value more than 0.5, confirms their superiority in detection of polymorphism among studied germplasm lines. UPGMA analysis assemblage all the germplasm lines into three major clusters. Most of the foliar disease resistant genotypes were grouped together with higher genetic resemblance. Polymorphic markers identified in the study may be utilized further for molecular diversity analysis and the identified resistant genotypes may be employed further for the improvement of the crop.
Akash Sharma, M.K. Tripathi, Sushma Tiwari, Neha Gupta, Niraj Tripathi, and Nishi Mishra
Agricultural Research Communication Center
Background: Soybean is an important leguminous crop. Abnormal weather has played an enormous role in the strident decline in crop yields. Drought is considered as a significant abiotic factor responsible for yield reduction in soybean. Methods: The present work was carried out in order to screen soybean genotypes for their drought tolerance ability by means of different biochemical and antioxidant enzymatic activities responses. Conclusion: On the basis of biochemical parameters and anti-oxidant enzymatic activities, soybean genotype viz., RVS-211-77, RVS-211-75, NRC-7, SL-96, NRC-136, AMS100-39, SL-96, RVS-2012-01, RVS-211-73 and JS97-52 have been identified with better performance and can be used as parents for further crop improvement programme to breed drought tolerant variety.
Chitralekha Shyam, Manoj Kumar Tripathi, Sushma Tiwari, Niraj Tripathi, Ravindra Singh Solanki, Swapnil Sapre, Ashok Ahuja, and Sharad Tiwari
MDPI AG
Brassica juncea is a crucial cultivated mustard species and principal oilseed crop of India and Madhya Pradesh, grown for diverse vegetables, condiments, and oilseeds. Somaclonal variation was explored as a probable source of additional variability for the manipulation of fatty acids, especially low erucic acid contents that may be valuable for this commercially important plant species. The plantlets regenerated from tissue cultures (R0), their R1 generation and respective parental lines were compared for morpho-physiological traits and fatty acid profile for the probable existence of somaclonal variations. The first putative somaclone derived from genotype CS54 contained 5.48% and 5.52% erucic acid in R0 and R1 regenerants, respectively, compared to the mother plant (41.36%). In comparison, the second somaclone acquired from PM30 exhibited a complete absence of erucic acid corresponding to its mother plant (1.07%). These putative somaclones present a source of variation for exploitation in the development of future mustard crops with low erucic acid content.
Manoj Kumar Tripathi, Niraj Tripathi, Sushma Tiwari, Gyanendra Tiwari, Nishi Mishra, Dilip Bele, Rajesh Prasad Patel, Swapnil Sapre, and Sharad Tiwari
MDPI AG
Santalum album (L.) is a prized tropical tree species of high therapeutic and industrial importance. The wood of these naturally grown plants is extensively harvested to acquire therapeutically important metabolite santalol and be used for additional functions such as in wood statuette industries. Due to high demand, it is crucial to maintain a sufficient plant population. An easy protocol for establishing cell suspension culture initiated from the loose embryogenic callus mass of sandalwood was realized by shifting 6–8-week-old morphogenic calli acquired from the mature embryonic axis and cotyledon explant cultures in fluid media. The asynchronous embryogenic cultures were sloughed with clumps of flourishing cell clumps and embryos of various progressive phases along with diffident non-embryogenic tissues. The frequency of embryo proliferation was evidenced to determinethe expansion pace of embryogenic masses under diverse conditions. The intonation of initiation and creation of cell suspension was under the directive of the influence of exogenous plant growth regulators amended in the nutrient medium at different concentrations and combinations. Maximum relative growth rate (386%) and clumps/embryoids in elevated integers (321.44) were accomplished on MS nutrient medium fortified with 2.0 mg L−1 2,4-D in association with 0.5 mg L−1 BA and 30.0 g L−1 sucrose raised from mature embryonic axis-derived calli. Plantlet regeneration in higher frequency (84.43%) was evidenced on MS medium amended with 1.0 mg L−1 each of TDZ and GA3 in conjunction with 0.5 mg L−1 NAA and 20.0 g L−1 sucrose. Mature embryonic axis-derived calli were found to be constantly better than mature cotyledon-derived calli for raising profitable and reproducible cell suspension cultures. Regenerants displayed normal growth and morphology and were founded successfully in the external environment after hardening.