D.D.S (General Dentistry) mashhad medical science University, Dental school
M.S. in Periodontics( shahid beheshti Medical science University, Dental School
Digitally Guided Hydraulic Crestal Sinus Floor Elevation Versus Free-Hand Osteotome Technique: A Single-Blinded Randomized Controlled Clinical Trial Mahsa Enssi, Mohammadreza Karimi, Ardavan Etemadi, Mahsa Khademi, Hamoun Sabri Clinical Oral Implants Research, 2026 Objectives To compare the clinical and radiographic outcomes of crestal sinus floor elevation (SFE) using a digital surgical guide (DSG) and hydraulic sinus lift technique versus the conventional osteotome technique. Methods Patients requiring crestal SFE and single‐implant placement in the posterior maxilla with a minimum of 5 mm of residual bone height were included and randomly assigned to two groups of crestal SFE with (i) the conventional technique (elevation of a mucoperiosteal flap and SFE by the osteotome technique, control group) and (ii) by using a DSG (without flap elevation, by using the sinus lift hydraulic system in the presence of a DSG, test group). Frequency of intraoperative membrane perforation, surgical time, and patient‐reported outcomes were collected. Crestal bone loss at 6 months, new bone formation in the sinus, and implant position were assessed. Results Twenty‐three patients (24 implants, 12 in each group) were included. The DSG group experienced significantly lower postoperative pain (measured by the number of analgesics taken, 1.67 ± 0.77 vs. 2.75 ± 1.21, p = 0.028), shorter surgical time (22.3 ± 2.4 vs. 33.1 ± 4.1 min, p < 0.001), and smaller deviation of implant angulation from the ideal position (2.9° ± 0.6° vs. 8.6° ± 1.3°, p < 0.001) compared to the conventional group. The difference in other parameters was not significant. Conclusion Considering the study limitations, using a DSG with a hydraulic system for crestal SFE was superior to the conventional technique regarding pain score, surgical time, and implant angulation. Due to the relatively small sample size and lack of statistically significant differences in some parameters, these findings should be interpreted with caution. Trial Registration Iranian Registry of Clinical Trials: IRCT20230529058333N1
The photobiomodulation effects of continuous and pulsed blue diode laser on proliferation and osteogenic differentiation of periodontal ligament stem cells Mohammadreza Karimi, Mahsa Sheibani Pour, Ardavan Etemadi, Mohammad Amin Karimi, Mahshid Hodjat, Nasim Chiniforush Photochemistry and Photobiology, 2026 This study investigated the photobiomodulation effect of pulsed and continuous blue diode laser on osteogenic differentiation and proliferation of periodontal ligament mesenchymal stem cells. Periodontal Ligament Stem cells were seeded in 96‐well plates, and 450 nm blue laser irradiation procedure was performed a day after cell seeding. Each experimental group was divided into two subgroups according to their energy density and irradiation duration: Continuous wave (100 mW, 10s, 2 J/cm2 and 100 mW, 20 s, 4 J/cm2) and pulse wave (200 mW, 10 s, 2 J/cm2 and 200 mW, 20 s, 4 J/cm2 and duty cycle 50% for both). Then, all groups were evaluated with a cell viability test (MTT), cell apoptosis (Annexin V) on the second and fourth days after irradiation, Alizarin Red staining on the 14th day after irradiation based on genes. Real‐time PCR was conducted 7 and 14 days after irradiation. GAPD gene primers were used as internal control, and OPN, OCN, ALP, and RUNX2 gene primers were used as tests. The one‐way ANOVA statistical analysis revealed that cell proliferation in the continuous‐irradiated groups was significantly higher than in pulsed groups. However, there is no significant difference in comparison with the control group. Also, pulsed‐irradiated groups demonstrated a higher rate of necrosis. The osteogenic differentiation in the continuous groups was more substantial than in the pulsed and the control groups. In comparison to all other study groups, the group that received continuous mode irradiation at an energy density of 2 J/cm2, power of 100 mW, and a radiation time of 10 s exhibited significantly higher numbers of calcified nodules and increased expression of OPN, OCN, and ALP genes (p < 0.05). Overall, treating periodontal ligament stem cells with a continuous blue diode laser and appropriate parameters can enhance their osteogenic differentiation and proliferation, accelerating the regeneration of periodontal tissues.
Efficacy of antimicrobial photodynamic therapy with chitosan nanoparticles for decontamination of dental implants contaminated with Aggregatibacter actinomycetemcomitans Ferena Sayar, Mohammad Reza Karimi, Sepehr Boroujerdi Scientific Reports, 2025 This study aimed to assess the efficacy of antimicrobial photodynamic therapy (aPDT) with chitosan nanoparticles (CNPs) for decontamination of dental implants inoculated with Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). In this in vitro study, CNPs containing indocyanine green (ICG) were synthesized and characterized. Sandblasted large-grit acid-etched (SLA) titanium implants (n = 54) were inoculated with A. actinomycetemcomitans, and randomly assigned to 6 groups (n = 9) for decontamination: (I) negative control (PBS rinse for 60 s), (II) positive control [exposure to 0.2% chlorhexidine (CHX) for 5 min], (III) exposure to 0.25 mg/mL ICG at 37 °C for 5 min, (IV) exposure to 0.25 mg/mL ICG followed by 808 nm diode laser irradiation for 60 s, (V) exposure to 0.25 mg/mL ICG-loaded CNPs, (VI) exposure to 0.25 mg/mL ICG-loaded CNPs followed by 808 nm diode laser irradiation for 60 s. The A. actinomycetemcomitans colonies were counted, and data were analyzed by one-way ANOVA and Tamhane test (alpha = 0.05). The test groups showed a significant reduction in colony count compared to the negative control (P < 0.05). Laser plus ICG-loaded CNPs and CHX had comparably the highest decontamination efficacy. This study demonstrated that aPDT with ICG-loaded CNPs significantly reduced the A. actinomycetemcomitans colony count on dental implants, showing comparable efficacy to CHX.
A comparative evaluation of the effects of 635 nm laser on cell proliferation and osteogenic differentiation of buccal fat pad mesenchymal stem cells Ardavan Etemadi, Mohammad Amin Karimi, Mohammadreza Karimi, Mahshid Hodjat, Mahsa Sheibani Pour, Alireza Karimi, Nasim Chiniforush Photochemistry and Photobiology, 2025 The purpose of this study was to evaluate the effects of 635 nm diode laser with different powers on undifferentiated mesenchymal stem cells obtained from buccal fat pad. Human buccal fat stem cells were cultured in DMEM containing 10% FBS, penicillin, and streptomycin under 5% CO2 and 95% humidity. Cells were cultured in 96‐well plate and 24 h later, laser irradiation with 635 nm diode laser was performed in four groups of 200, 300, 400, and 500 mW powers in addition to the control group with the same energy density of 4 J/cm2. MTT and flow cytometry assay was performed to evaluate cell proliferation and viability on 2 and 4 days after irradiation. Alizarin red assay and real‐time PCR (OPN, OCN, ALP, and RUNX‐2 genes) was performed to evaluate osteogenic differentiation. According to the MTT assay, none of the mentioned powers of 635 nm diode laser had significant effect on cell proliferation. Cells irradiated with power of 400 mW and 500 mW significantly showed a greater number of necrotic cells compared to the control group in Day 4. Cells irradiated with 300 mW power significantly exhibited a greater amount of nodule formation compared to all groups. Results of this study indicated that 635 nm diode laser with energy density of 4 J/cm2 has a positive effect inducing osteogenic differentiation when applying with a power of 300 mW in buccal fat pad mesenchymal stem cells.
Investigating the Effect of Photobiomodulation Therapy With Different Wavelengths of Diode Lasers on the Proliferation and Adhesion of Human Gingival Fibroblast Cells to a Collagen Membrane: An In Vitro Study Mohammad Reza Karimi, Shahrzad Abdollahi, Ardavan Etemadi, Neda Hakimiha Journal of Lasers in Medical Sciences, 2024 Introduction: Photobiomodulation (PBM) is considered a promising adjunctive approach in regenerative medicine. This study aimed to investigate the proliferation and adhesion of human gingival fibroblast (HGF) cells to a collagen membrane following PBM. Methods: Cultured HGF cells on a collagen membrane received PBM at wavelengths of 808 nm, 915 nm (2 and 4 J/cm2 ), and 660 nm (2.1 and 4.2 J/cm2 ) in interventional groups, while non-irradiated cells served as the control. On days 1 and 3 post-irradiation, cell proliferation was measured by MTT assay, and adhesion to the membrane was assessed under the scanning electron microscope (SEM). Results: Cell proliferation significantly increased in interventional groups compared to the control, with the most significant increase at 915 nm (4 J/cm2 ) in both time points. On the first day, the 808 nm and 660 nm lasers demonstrated similar results, significantly lower than the 915 nm laser (2 J/cm2). On day 3, the 660 nm, 808 nm, and 915 nm (2 J/cm2) groups showed comparable results. Qualitative analysis by the SEM identified spindle-shaped cells with multiple extended projections in 915 nm groups, especially at an energy density of 4 J/cm2 . Groups of 660 nm and 808 nm (4 J/cm2 ) showed spindle-shaped cell morphology. No distinct cellular morphology indicative of enhanced adhesion was observed at 808 nm (2 J/cm2). Conclusion: The most effective PBM setup for promoting HGF proliferation and adhesion to a collagen membrane was identified at 915 nm (4 J/cm2 ).
Reduction of Streptococcus salivarius by ChlorellaMediated Antimicrobial Photodynamic Therapy Mohammad Reza Karimi, Navid Pourahmad, Shima Afrasiabi, Ardavan Etemadi, Nasim Chiniforush Journal of Lasers in Medical Sciences, 2024 Introduction: Nowadays, antimicrobial photodynamic therapy (aPDT) has been introduced as one of the minimally invasive methods for disinfection of the surfaces of dental implants. Being derived from seaweed, Chlorella has been used as a photosensitizer in this study. This study aimed to investigate the impacts of aPDT with Chlorella on the rate of reduction of Streptococcus salivarius in vitro. Methods: The minimum inhibitory concentration of Chlorella, the sublethal exposure to 660 nm diode laser irradiation, and the minimum sublethal dose of aPDT utilizing Chlorella against S. salivarius were determined. Finally, the CFU/mL value of each plate was calculated. Then, Tukey HSD and one-way ANOVA tests were utilized for comparison the number of colonies after the interventions. Results: A concentration of 250 µg/mL of Chlorella at an irradiation time of 3 minutes, was identified as a sublethal dose of aPDT for the reduction of S. salivarius. In contrast, the application of aPDT utilizing a 660 nm diode laser for 4 minutes in combination with Chlorella at a final concentration of 500 µg/mL, demonstrated significantly greater efficacy in reducing S. salivarius compared to the other experimental groups (P<0.001). Conclusion: Chlorella 500 µg/mL mediated aPDT (660 nm, 4 minutes) has a significant effect on reducing S. salivarius count.
Attachment and proliferation of human gingival fibroblasts seeded on barrier membranes using Wharton's jelly-derived stem cells conditioned medium: An in vitro study Mohammadreza Karimi, Seyed Ali Mosaddad, Seyedeh Sara Aghili, Hengameh Dortaj, Seyedeh‐Sara Hashemi, Farin Kiany Journal of Biomedical Materials Research Part B Applied Biomaterials, 2024 The effect of Wharton's jelly mesenchymal stem cells conditioned medium (WJMSCs‐CM) and zinc oxide nanoparticles (ZnO‐NPs) on cultured human gingival fibroblasts on various barrier membranes was investigated in this study. In this study, human gingival fibroblasts were prepared and cultured on three membranes: collagen membrane, acellular dermal matrix (ADM) with ZnO‐NPs, and ADM without ZnO‐NPs. WJMSCs‐CM was given to the testing groups, while control groups received the same membranes without WJMSCs‐CM. Following 48 and 72 h, 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) tests were performed to assess cell survival. Cell proliferation on the membranes was also evaluated using 4′,6‐diamidino‐2‐phenylindole (DAPI) staining after 48 and 72 h. Field emission scanning electron microscopy was used to determine membrane surface structure and cell adhesion. Nanoparticles were also subjected to an energy‐dispersive x‐ray analysis to identify their chemical structure. Two‐way analysis of variance was used to conduct the statistical analysis. The p‐value ≤.05 was considered significant. When ADM‐ZnO‐NPs were combined with CM, fibroblast viability, and adhesion significantly differed from ADM‐ZnO‐NPs alone. DAPI results confirmed cell proliferation in all six groups on both experiment days. The abundance and concentrated distribution of cells during cell proliferation were found in CM‐containing membranes, specifically the ADM‐ZnO‐NPs membrane, demonstrating the improved biocompatibility of the ADM‐ZnO‐NPs membrane for cell proliferation. The other groups did not significantly differ from one another. WJMSCs‐CM positively affected the viability and proliferation of gingival fibroblasts, but only marginally. Under certain conditions, ZnO‐NPs below a specific concentration increased the biocompatibility of the membranes.
Oral Complications Associated with the Piercing of Oral and Perioral Tissues and the Corresponding Degree of Awareness among Public and Professionals: A Systematic Review Seyed Mosaddad, Sahar Talebi, Maryam Hemmat, Mohammadreza Karimi, Alireza Jahangirnia, Mostafa Alam, Kamyar Abbasi, Mohsn Yazadaniyan, Ahmed Hussain, Hamid Tebyaniyan, Reza Abdollahi Namanloo Diagnostics, 2023 This study systematically reviews the literature to evaluate the potential relationships between oral/perioral piercing and consequent oral complications in the corresponding society. The second objective was determining public/professional sectors’ awareness of the subject. This research followed PRISMA and Cochrane guidelines for conducting systematic reviews and searching scientific databases, including PubMed, Scopus, Cochrane, and Google Scholar, until April 2023. Cross-sectional, cohort, and case–control studies in English were deemed eligible. The methodological quality of the included studies was assessed using proper quality assessment guidelines. Of the 965 initial articles retrieved, 34 were considered suitable for qualitative synthesis after screening procedures and removing duplicates and irrelevant records. There appears to be an imbalance between the general public’s low and dentists’ high awareness. This draws attention to the shortage of professional and societal knowledge-sharing and education initiatives. Women were more than twice as likely as men to have oral piercings. Piercing usage had a low incidence among a cohort of students with a mean age of 16. Merely circumstantial evidence has indicated a plausible correlation between oral and perioral piercings and the emergence of secondary bacterial and fungal colonization, particularly periodontopathogenic bacteria and Candida albicans. Furthermore, several adverse consequences have been observed linked to various piercings—such as lip and tongue piercings. These include caries, gingivitis/periodontitis, dental fractures, enamel chipping/cracks, plaque buildup, bone loss, bleeding, inflammation, and swelling. Given the risks involved and the complications that might impair oral health, the prevalence of oral piercings is alarming. As a result, public health authorities need to firmly support initiatives to raise awareness of the risks associated with oral/perioral piercings. For piercers to enhance their expertise in this field, professional training is necessary because there is a shortage of knowledge on the possible adverse effects of piercings.
Comparison of manual tools, Ultrasonic and Erbium-Doped Yttrium Aluminum Garnet (Er: YAG) Laser on the debridement effect of the surface of the root of teeth suffering from periodontitis Journal of Lasers in Medical Sciences, 2013
RECENT SCHOLAR PUBLICATIONS
Efficacy of titanium brush, 915 nm diode laser, citric acid for eradication of Staphylococcus aureus from implant surfaces MR Karimi, B Farkhondemehr, M Ghaeni Najafi, A Etemadi, N Chiniforush BMC Oral health 21 (1), 631 , 2021 2021 Citations: 7
What's better for children; an electric or manual toothbrush M Karimi Interv Pediatr Dent Open Access J 5, 475-78 , 2021 2021 Citations: 4
Dental Implant in Children: A Controversy issue M Karimi Clinical Oral Science and Dentistry 2, 3 , 2019 2019 Citations: 1
Ways to Encourage Children to Brush Their Teeth M Karimi CPQ Dentistry 1, 01-06 , 2019 2019 Citations: 2
Effect of photodynamic therapy using toluidine blue on Eikenella corrodens and Aggregatibacter actinomycetemcomitans biofilms adhered to titanium discs: an in vitro study MR Karimi, M Montazeri, M Harandi, L Aghazadeh Journal of Research in Dental and Maxillofacial Sciences 3 (4), 18-25 , 2018 2018 Citations: 6
The Comparison between effects of the injection rate in infiltration technique on pain, anxiety and vital signs of the patients using ICT injection MR Karimi, HR MAHASENI MOGHADAM, SH Kiaee Res Dent Sci 15 (3), 125-129 , 2018 2018
The association between serum vitamin D level with breast cancer and ER/PR, Her-2 in north of Iran F Nemati, S Siadati, M Karimi, S Khafri, S Kamali Ahangar, ... Iranian Journal of Surgery 26 (Spring), 38-46 , 2018 2018 Citations: 2
The effect of photodynamic therapy on SLA Titanium discs biocompatibility MR Karimi, N Jahanfar, CH AZIMI, Z Ghasemifar Res Dent Sci 14 (4), 206-212 , 2018 2018
The Proper Toothpaste for Children M Karimi J. Dental Sci 3 (3), 195 , 2018 2018 Citations: 4
Stains on Children's teeth and the treatment M Karimi 2018
Relationship between stress, anxiety, depression and salivary IgA with periodontal disease M Karimi, S Elyahoo, L Golchin, T Kermani Bioscience Biotechnology Research Communications 10 (2), 88-92 , 2017 2017 Citations: 7
Efficacy of antimicrobial photodynamic therapy as an adjunctive to mechanical debridement in the treatment of peri-implant diseases: a randomized controlled clinical trial MR Karimi, A Hasani, S Khosroshahian Journal of lasers in medical sciences 7 (3), 139 , 2016 2016 Citations: 76
Grandma remedies and herbal medicines for relieving toothache M Karimi Open Access Journal of Dental Sciences 1 (1), 000106 , 2016 2016 Citations: 1
The causes of Holitosis in children M Karimi Pediatr Dent Care 2, 1 , 2016 2016 Citations: 4
An in vitro evaluation of the responses of human osteoblast-like SaOs-2 cells on SLA titanium surfaces irradiated by different powers of CO 2 lasers N Ayubianmarkazi, M Karimi, S Koohkan, A Sanasa, T Foroutan Lasers in medical science 30 (8), 2129-2134 , 2015 2015 Citations: 7
The relationship between maternal periodontal status of and preterm and low birth weight infants in Iran: a case control study MR Karimi, JH Hamissi, SR Naeini, M Karimi Global journal of health science 8 (5), 184 , 2015 2015 Citations: 65
Effects of counter torque and transposition (transfer) of installed implants timing on their integration in dog tibia MR Karimi, S Fathi, F Ghanavati The Journal of Advanced Prosthodontics 7 (1), 62-68 , 2015 2015 Citations: 3
Effects of Er: YAG laser irradiation on wettability, surface roughness, and biocompatibility of SLA titanium surfaces: an in vitro study N Ayobian-Markazi, M Karimi, A Safar-Hajhosseini Lasers in medical science 30 (2), 561-566 , 2015 2015 Citations: 57
Effect of dental floss impregnated with SUAB2 solution in patients with periodontal disease M Naseri, MR Karimi, MR Behtoyi تحقيق در علوم دندانپزشکي , 2014 2014
Effect of SUAB2 impregnated dental floss on periodontal indices MR Karimi, M Naseri, MR Behtoei Res Dent Sci 10 (4), 228-234 , 2014 2014 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
Efficacy of antimicrobial photodynamic therapy as an adjunctive to mechanical debridement in the treatment of peri-implant diseases: a randomized controlled clinical trial MR Karimi, A Hasani, S Khosroshahian Journal of lasers in medical sciences 7 (3), 139 , 2016 2016 Citations: 76
The relationship between maternal periodontal status of and preterm and low birth weight infants in Iran: a case control study MR Karimi, JH Hamissi, SR Naeini, M Karimi Global journal of health science 8 (5), 184 , 2015 2015 Citations: 65
Effects of Er: YAG laser irradiation on wettability, surface roughness, and biocompatibility of SLA titanium surfaces: an in vitro study N Ayobian-Markazi, M Karimi, A Safar-Hajhosseini Lasers in medical science 30 (2), 561-566 , 2015 2015 Citations: 57
Comparison of manual tools, ultrasonic and erbium-doped yttrium aluminum garnet (Er: YAG) laser on the debridement effect of the surface of the root of teeth suffering from … T Foroutan, R Amid, MR Karimi Journal of lasers in medical sciences 4 (4), 199 , 2013 2013 Citations: 17
Efficacy of titanium brush, 915 nm diode laser, citric acid for eradication of Staphylococcus aureus from implant surfaces MR Karimi, B Farkhondemehr, M Ghaeni Najafi, A Etemadi, N Chiniforush BMC Oral health 21 (1), 631 , 2021 2021 Citations: 7
Relationship between stress, anxiety, depression and salivary IgA with periodontal disease M Karimi, S Elyahoo, L Golchin, T Kermani Bioscience Biotechnology Research Communications 10 (2), 88-92 , 2017 2017 Citations: 7
An in vitro evaluation of the responses of human osteoblast-like SaOs-2 cells on SLA titanium surfaces irradiated by different powers of CO 2 lasers N Ayubianmarkazi, M Karimi, S Koohkan, A Sanasa, T Foroutan Lasers in medical science 30 (8), 2129-2134 , 2015 2015 Citations: 7
Effect of photodynamic therapy using toluidine blue on Eikenella corrodens and Aggregatibacter actinomycetemcomitans biofilms adhered to titanium discs: an in vitro study MR Karimi, M Montazeri, M Harandi, L Aghazadeh Journal of Research in Dental and Maxillofacial Sciences 3 (4), 18-25 , 2018 2018 Citations: 6
What's better for children; an electric or manual toothbrush M Karimi Interv Pediatr Dent Open Access J 5, 475-78 , 2021 2021 Citations: 4
The Proper Toothpaste for Children M Karimi J. Dental Sci 3 (3), 195 , 2018 2018 Citations: 4
The causes of Holitosis in children M Karimi Pediatr Dent Care 2, 1 , 2016 2016 Citations: 4
Effects of counter torque and transposition (transfer) of installed implants timing on their integration in dog tibia MR Karimi, S Fathi, F Ghanavati The Journal of Advanced Prosthodontics 7 (1), 62-68 , 2015 2015 Citations: 3
Ways to Encourage Children to Brush Their Teeth M Karimi CPQ Dentistry 1, 01-06 , 2019 2019 Citations: 2
The association between serum vitamin D level with breast cancer and ER/PR, Her-2 in north of Iran F Nemati, S Siadati, M Karimi, S Khafri, S Kamali Ahangar, ... Iranian Journal of Surgery 26 (Spring), 38-46 , 2018 2018 Citations: 2
A study on stress distribution pattern in implants with and without microthread, using FEM MR KARIMI, A KAMALI, M Mahzari, S Borhani Journal of Research in Dental Sciences 7 (123), 43-51 , 2010 2010 Citations: 2
Dental Implant in Children: A Controversy issue M Karimi Clinical Oral Science and Dentistry 2, 3 , 2019 2019 Citations: 1
Grandma remedies and herbal medicines for relieving toothache M Karimi Open Access Journal of Dental Sciences 1 (1), 000106 , 2016 2016 Citations: 1
Effect of SUAB2 impregnated dental floss on periodontal indices MR Karimi, M Naseri, MR Behtoei Res Dent Sci 10 (4), 228-234 , 2014 2014 Citations: 1
CFD Investigation of Interface Location in Stirred Tanks with a Concave Impeller P Parvasi, R Janamiri, A Sinkakarimi, I Mahdavi, M Safdari, MH Sedaghat, ... World Academy of Science, Engineering and Technology 7 (6) , 2013 2013 Citations: 1
Interface Location in Single Phase Stirred Tanks I Mahdavi, R Janamiri, A Sinkakarimi, M Safdari, MH Sedaghat, A Zamani, ... Int. J. Chem. Mol. Eng 7 (6) , 2013 2013 Citations: 1
