Gallinelli Andrea
@ausl.fe.it
Scopus Publications
- Do faster, do better: frozen embryo transfer outcomes with one-step warming protocol at different embryos stages
Rossella Fucci, Patrizia Falcone, Francesco Capodanno, Sara Rubini, Andrea Gallinelli, Vincenzo Lofiego, Silvia De Stefani, Mariangela Primiterra, Elisabetta Coccia, Elisabetta Baldi, Simone Palini
Reproductive Biomedicine Online, 2025 - Current and future methods for embryo selection: on a quest for reliable strategies to reduce time to pregnancy
Francesco CAPODANNO, Attilio ANASTASI, Marialuisa CINTI, Francesca BONESI, Andrea GALLINELLI
Minerva Obstetrics and Gynecology, 2024
INTRODUCTION The aim of this study was to analyze the usefulness of the principal embryological strategies to reduce time to pregnancy. EVIDENCE ACQUISITION A systematic search of publications in the PubMed/MEDLINE, Embase and Scopus databases from inception to present including "IVF," "blastocyst," "embryo colture," "competent embryo," "time to pregnancy," "aneuploid," "euploid," "vitrification," "preimplantation genetic," "IVF strategies" and "embryo selection" alone or in combinations has been done. EVIDENCE SYNTHESIS We have selected 230 articles and 9 of them have been included in this mini-review. CONCLUSIONS Several embryological strategies aimed to select the most competent embryo and reduce time to pregnancy have been proposed, even if few publications on this specific topic are available. preimplantation genetic testing (PGT-A) represents the unique method able to assess the embryonic chromosomal status, but this does not mean that PGT-A is a reliable strategy to reduce time to pregnancy. There is no consensus on a specific method to reduce time to pregnancy, nevertheless this final goal could be probably reached through a harmonious combination of procedures. Thus, a reliable strategy to reduce time to pregnancy could be achieved when embryo culture, embryo cryopreservation and PGT-A are perfectly integrated and appropriately offered to selected patients. - Improved clinical utility of preimplantation genetic testing through the integration of ploidy and common pathogenic microdeletions analyses
S Caroselli, M Figliuzzi, L Picchetta, F Cogo, P Zambon, I Pergher, L Girardi, C Patassini, M Poli, D Bakalova, D Cimadomo, N Findikli, O Coban, M Serdarogullari, F Favero, S Bortolato, A Anastasi, F Capodanno, A Gallinelli, F Brancati, L Rienzi, F M Ubaldi, J Jimenez-Almazán, D Blesa-Jarque, J Miravet-Valenciano, C Rubio, C Simòn, A Capalbo
Human Reproduction, 2023
STUDY QUESTIONCan chromosomal abnormalities beyond copy-number aneuploidies (i.e. ploidy level and microdeletions (MDs)) be detected using a preimplantation genetic testing (PGT) platform?SUMMARY ANSWERThe proposed integrated approach accurately assesses ploidy level and the most common pathogenic microdeletions causative of genomic disorders, expanding the clinical utility of PGT.WHAT IS KNOWN ALREADYStandard methodologies employed in preimplantation genetic testing for aneuploidy (PGT-A) identify chromosomal aneuploidies but cannot determine ploidy level nor the presence of recurrent pathogenic MDs responsible for genomic disorders. Transferring embryos carrying these abnormalities can result in miscarriage, molar pregnancy, and intellectual disabilities and developmental delay in offspring. The development of a testing strategy that integrates their assessment can resolve current limitations and add valuable information regarding the genetic constitution of embryos, which is not evaluated in PGT providing new level of clinical utility and valuable knowledge for further understanding of the genomic causes of implantation failure and early pregnancy loss. To the best of our knowledge, MDs have never been studied in preimplantation human embryos up to date.STUDY DESIGN, SIZE, DURATIONThis is a retrospective cohort analysis including blastocyst biopsies collected between February 2018 and November 2021 at multiple collaborating IVF clinics from prospective parents of European ancestry below the age of 45, using autologous gametes and undergoing ICSI for all oocytes. Ploidy level determination was validated using 164 embryonic samples of known ploidy status (147 diploids, 9 triploids, and 8 haploids). Detection of nine common MD syndromes (-4p=Wolf-Hirschhorn, -8q=Langer-Giedion, -1p=1p36 deletion, -22q=DiGeorge, -5p=Cri-du-Chat, -15q=Prader-Willi/Angelman, -11q=Jacobsen, -17p=Smith-Magenis) was developed and tested using 28 positive controls and 97 negative controls. Later, the methodology was blindly applied in the analysis of: (i) 100 two pronuclei (2PN)-derived blastocysts that were previously defined as uniformly euploid by standard PGT-A; (ii) 99 euploid embryos whose transfer resulted in pregnancy loss.PARTICIPANTS/MATERIALS, SETTING, METHODSThe methodology is based on targeted next-generation sequencing of selected polymorphisms across the genome and enriched within critical regions of included MD syndromes. Sequencing data (i.e. allelic frequencies) were analyzed by a probabilistic model which estimated the likelihood of ploidy level and MD presence, accounting for both sequencing noise and population genetics patterns (i.e. linkage disequilibrium, LD, correlations) observed in 2504 whole-genome sequencing data from the 1000 Genome Project database. Analysis of phased parental haplotypes obtained by single-nucleotide polymorphism (SNP)-array genotyping was performed to confirm the presence of MD.MAIN RESULTS AND THE ROLE OF CHANCEIn the analytical validation phase, this strategy showed extremely high accuracy both in ploidy classification (100%, CI: 98.1–100%) and in the identification of six out of eight MDs (99.2%, CI: 98.5–99.8%). To improve MD detection based on loss of heterozygosity (LOH), common haploblocks were analyzed based on haplotype frequency and LOH occurrence in a reference population, thus developing two further mathematical models. As a result, chr1p36 and chr4p16.3 regions were excluded from MD identification due to their poor reliability, whilst a clinical workflow which incorporated parental DNA information was developed to enhance the identification of MDs. During the clinical application phase, one case of triploidy was detected among 2PN-derived blastocysts (i) and one pathogenic MD (-22q11.21) was retrospectively identified among the biopsy specimens of transferred embryos that resulted in miscarriage (ii). For the latter case, family-based analysis revealed the same MD in different sibling embryos (n = 2/5) from non-carrier parents, suggesting the presence of germline mosaicism in the female partner. When embryos are selected for transfer based on their genetic constitution, this strategy can identify embryos with ploidy abnormalities and/or MDs beyond aneuploidies, with an estimated incidence of 1.5% (n = 3/202, 95% CI: 0.5–4.5%) among euploid embryos.LIMITATIONS, REASONS FOR CAUTIONEpidemiological studies will be required to accurately assess the incidence of ploidy alterations and MDs in preimplantation embryos and particularly in euploid miscarriages. Despite the high accuracy of the assay developed, the use of parental DNA to support diagnostic calling can further increase the precision of the assay.WIDER IMPLICATIONS OF THE FINDINGSThis novel assay significantly expands the clinical utility of PGT-A by integrating the most common pathogenic MDs (both de novo and inherited ones) responsible for genomic disorders, which are usually evaluated at a later stage through invasive prenatal testing. From a basic research standpoint, this approach will help to elucidate fundamental biological and clinical questions related to the genetics of implantation failure and pregnancy loss of otherwise euploid embryos.STUDY FUNDING/COMPETING INTEREST(S)No external funding was used for this study. S.C., M.F., F.C., P.Z., I.P., L.G., C.P., M.P., D.B., J.J.-A., D.B.-J., J.M.-V., and C.R. are employees of Igenomix and C.S. is the head of the scientific board of Igenomix. A.C. and L.P. are employees of JUNO GENETICS. Igenomix and JUNO GENETICS are companies providing reproductive genetic services.TRIAL REGISTRATION NUMBERN/A. - Diagnostic accuracy of sonohysterography and transvaginal sonography as compared with hysteroscopy and endometrial biopsy: A prospective study
Minerva Ginecologica, 2011 - Nitric oxide as an early marker of human embryo metabolic cleavage in ART using fresh or thawed oocytes
Andrea Gallinelli, Alessia Nicoli, Francesco Capodanno, Barbara Valli, Fabio Facchinetti, Giovanni B. La Sala
European Journal of Obstetrics and Gynecology and Reproductive Biology, 2009 - Limited importance of pre-embryo pronuclear morphology (zygote score) in assisted reproduction outcome in the absence of embryo cryopreservation
Alessia Nicoli, Barbara Valli, Roberta Di Girolamo, Barbara Di Tommaso, Andrea Gallinelli, Giovanni B. La Sala
Fertility and Sterility, 2007 - Natural history of multiples conceived by in vitro fertilization
Multiple Pregnancy Epidemiology Gestation and Perinatal Outcome, 2007 - Outcome of 518 salvage oocyte-cryopreservation cycles performed as a routine procedure in an in vitro fertilization program
Giovanni B. La Sala, Alessia Nicoli, Maria T. Villani, Michela Pescarini, Andrea Gallinelli, Isaac Blickstein
Fertility and Sterility, 2006
Cryopreservation performed as a routine procedure for so-called salvaging of surplus oocytes is associated with poor implantation rates and with a probability of 1 live birth in 65 ET cycles. - Effect of the mode of assisted reproductive technology conception on obstetric outcomes for survivors of the vanishing twin syndrome
Giovanni B. La Sala, Maria Teresa Villani, Alessia Nicoli, Andrea Gallinelli, Giuseppe Nucera, Isaac Blickstein
Fertility and Sterility, 2006 - Pregnancy loss and assisted reproduction: Preliminary results after the law 40/2004 in Italy
Giovanni Battista La Sala, Andrea Gallinelli, Alessia Nicoli, Maria Teresa Villani, Giuseppe Nucera
Reproductive Biomedicine Online, 2006 - Spontaneous embryonic loss rates in twin and singleton pregnancies after transfer of top- versus intermediate-quality embryos
Giovanni B. La Sala, Alessia Nicoli, Maria Teresa Villani, Andrea Gallinelli, Giuseppe Nucera, Isaac Blickstein
Fertility and Sterility, 2005 - Lower embryonic loss rates among twin gestations following assisted reproduction
G. B. La Sala, G. Nucera, A. Gallinelli, A. Nicoli, M. T. Villani, I. Blickstein
Journal of Assisted Reproduction and Genetics, 2005 - Natural history of multiples conceived by in vitro fertilization
Multiple Pregnancy Epidemiology Gestation and Perinatal Outcome, 2005 - Prevalence and determinants of genital shedding of herpes simplex virus among women attending Italian colposcopy clinics
Fausto Boselli, Giuseppe Chiossi, Marisa Bortolamasi, Andrea Gallinelli
European Journal of Obstetrics and Gynecology and Reproductive Biology, 2005 - Is early embryonic crowding responsible for premature delivery and low birth weight of singleton pregnancies conceived by assisted reproductive technology? [1] (multiple letters)
Richard P. Dickey, Steven N. Taylor, Peter Y. Lu, Belinda M. Sartor, Phillip H. Rye
American Journal of Obstetrics and Gynecology, 2005 - Spontaneous embryonic loss after in vitro fertilization with and without intracytoplasmic sperm injection
Giovanni B. La Sala, Giuseppe Nucera, Andrea Gallinelli, Alessia Nicoli, Maria Teresa Villani, Isaac Blickstein
Fertility and Sterility, 2004 - Major congenital defects in assisted reproduction
Cuadernos De Medicina Reproductiva, 2004 - Spontaneous embryonic loss following in vitro fertilization: Incidence and effect on outcomes
Giovanni B. La Sala, Giuseppe Nucera, Andrea Gallinelli, Alessia Nicoli, Maria Teresa Villani, Isaac Blickstein
American Journal of Obstetrics and Gynecology, 2004 - Developmental outcomes at one and two years of children conceived by intracytoplasmic sperm injection
International Journal of Fertility and Women S Medicine, 2004 - Different concentrations of interleukins in the peritoneal fluid of women with endometriosis: Relationships with lymphocyte subsets
A. Gallinelli, G. Chiossi, L. Giannella, T. Marsella, A. D. Genazzani, A. Volpe
Gynecological Endocrinology, 2004 - The Italian STD 'hot-line': Making people aware
FAUSTO BOSELLI, GIUSEPPE CHIOSSI, ANDREA GALLINELLI
Sexually Transmitted Diseases, 2003 - Correlations between concentrations of interleukin-12 and interleukin-13 and lymphocyte subsets in the follicular fluid of women with and without polycystic ovary syndrome
Andrea Gallinelli, Ivan Ciaccio, Luca Giannella, Michela Salvatori, Tiziana Marsella, Annibale Volpe
Fertility and Sterility, 2003 - Physiological levels of 1α, 25 dihydroxyvitamin D3 induce the monocytic commitment of CD34+ hematopoietic progenitors
Journal of Leukocyte Biology, 2002 - Immunological changes and stress are associated with different implantation rates in patients undergoing in vitro fertilization-embryo transfer
Andrea Gallinelli, Roberto Roncaglia, Maria Lucia Matteo, Ivan Ciaccio, Annibale Volpe, Fabio Facchinetti
Fertility and Sterility, 2001 - Autonomic and neuroendocrine responses to stress in patients with functional hypothalamic secondary amenorrhea
Andrea Gallinelli, Maria Lucia Matteo, Annibale Volpe, Fabio Facchinetti
Fertility and Sterility, 2000 - Hormonal replacement therapy and urinary problems as evaluated by ultrasound and color Doppler
C. Battaglia, M. Salvatori, S. Giulini, M. R. Primavera, A. Gallinelli, A. Volpe
Ultrasound in Obstetrics and Gynecology, 1999 - Variations of cardiac performance and inotropism in healthy postmenopausal women treated with estroprogestin replacement therapy
Andrea Gallinelli, Stefano Angioni, Maria Lucia Matteo, Pier Luigi Montaldo, Maria Antonietta Fenu, Annibale Volpe
Menopause, 1999 - Proopiomelanocortin (POMC) mRNA and POMC-derived peptides immunolocalization in the skin of Protopterus annectens, an African lungfish
M.A. Masini, M. Sturla, M. Pestarino, F. Facchinetti, A. Gallinelli, B.M. Uva
Peptides, 1999 - Acetyl salmon endorphin-like and interrenal stress response in male gilthead sea bream, Sparus aurata
Gilberto Mosconi, Andrea Gallinelli, Alberta Maria Polzonetti-Magni, Fabio Facchinetti
Neuroendocrinology, 1998 - Expression of pro-opiomelanocortin (POMC) in the cerebral ganglion and ovary of a protochordate
M.A. Masini, M. Sturla, A. Gallinelli, S. Candiani, F. Facchinetti, M. Pestarino
Peptides, 1998 - Increased anticardiolipin antibodies are positively related to the uterine artery pulsatility index in unexplained infertility
C. Battaglia, L. Sgarbi, M. Salvatori, N. Maxia, A. Gallinelli, A. Volpe
Human Reproduction, 1998 - Pro-opiomelanocortin (POMC) expression and immunolocalization of POMC- related peptides in the ovary of Protopterus annectens, an African lungfish
M.A Masini, M Sturla, M Pestarino, A Gallinelli, F Facchinetti, B.M Uva
Peptides, 1997 - Placental stress factors and human parturition
F. PETRAGLIA, T. SIMONCINI, P. FLORIO, A. GALLINELLI, A. GIUNTINI, R. GREMIGNI, A. VOLPE, A. R. GENAZZANI
Annals of the New York Academy of Sciences, 1997 - Episodic secretion of activin A in pregnant women
A Gallinelli, R Gallo, AD Genazzani, ML Matteo, A Caruso, TK Woodruff, F Petraglia
European Journal of Endocrinology, 1996 - Genetics: Expression of pro-opiomelanocortin gene in human ovarian tissue
A. Gallinelli, G. Garuti, M.L. Matteo, A.R. Genazzani, F. Facchinetti
Human Reproduction, 1995 - Impact of the l-arginine/nitric oxide system in pregnancy
Isabella Neri, Gian Carlo Di Renzo, Gaetano Caserta, Andrea Gallinelli, Fabio Facchinetti
Obstetrical and Gynecological Survey, 1995 - Abnormal concentration of maternal serum activin-A in gestational diseases
F. Petraglia
Journal of Clinical Endocrinology and Metabolism, 1995 - Hypertension in pregnancy: Changes in activin a maternal serum concentration
F. Petraglia, L. Aguzzoli, A. Gallinelli, P. Florio, M. Zonca, C. Benedetto, K. Woodruff
Placenta, 1995 - Steroid-protein interaction in human placenta
Felice Petraglia, Antonio Alberto de Micheroux, Pasquale Florio, Michela Salvatori, Andrea Gallinelli, Vito Cela, Marco Antonio Palumbo, Andrea Riccardo Genazzani
Journal of Steroid Biochemistry and Molecular Biology, 1995 - Beta-cell secretion in patients with thalassemia major
Minerva Pediatrica, 1995 - Activin at parturition: Changes of maternal serum levels and evidence for binding sites in placenta and fetal membranes
Obstetrics and Gynecology, 1994 - Pulsatile fluctuations of plasma‐gonadotropin‐releasing hormone and corticotropin‐releasing factor levels in healthy pregnant women
Felice Petraglia, Alessandro D. Genazzani, Lorenzo Aguzzoli, Andrea Gallinelli, Davide de Vita, Alessandro Caruso, Andrea R. Genazzani
Acta Obstetricia Et Gynecologica Scandinavica, 1994 - Local production and action of follistatin in human placenta
F. Petraglia
Journal of Clinical Endocrinology and Metabolism, 1994 - Secretion and Putative Role of Activin and CRF in Human Parturition
FELICE PETRAGLIA, PASQUALE FLORIO, ANDREA GALLINELLI, ANTONELLO A. DE MICHEROUX, ALESSANDRO FERRARI, DA VIDE DE VITA, LORENZO AGUZZOLI, ALESSANDRO D. GENAZZANI, COSTANTINO DI CARLO
Annals of the New York Academy of Sciences, 1994 - Ovarian Melanotropic Peptides in Two Teleostean Fishes
F. FACCHINETTI, A. POLZONETTI‐MAGNI, I. NERI, A. GALLINELLI, G. ALESSANDRINI, M. VALLARINO
Annals of the New York Academy of Sciences, 1993 - Acetyl‐L‐Carnitine Effect on Pituitary and Plasma β‐Endorphin Responsiveness to Different Chronic Intermittent Stressors
Bozena Bidzinska, Felice Petraglia, Stefano Angioni, Alessandro D. Genazzani, Mario Criscuoio, Guido Ficarra, Andrea Gallinelli, Gian Paolo Trentini, Andrea R. Genazzani
Journal of Neuroendocrinology, 1993 - Corticotropin-releasing hormone modulates cytokines release in cultured human peripheral blood mononuclear cells
Stefano Angioni, Felice Petraglia, Andrea Gallinelli, Andrea Cossarizza, Claudio Franceschi, Michela Muscettola, Alessandro D. Genazzani, Nicola Surico, Andrea R. Genazzani
Life Sciences, 1993 - Effect of different chronic intermittent stressors and acetyl-i-carnitine on hypothalamic β-endorphin and gnrh and on plasma testosterone levels in male rats
Bozena Bidzinska, Felice Petraglia, Stefano Angioni, Alessandro D. Genazzani, Mario Criscuolo, Guido Ficarra, Andrea Gallinelli, Gian Paolo Trentini, Andrea R. Genazzani
Neuroendocrinology, 1993 - Deficit of galanin-like immunostaining in the median eminence of adult hypothyroid rats
Luciana Giardino, Antonino Velardo, Andrea Gallinelli, Laura Calzà
Neuroendocrinology, 1992 - Pulsatile fluctuations of plasma-gonadotropin-releasing hormone and corticotropin-releasing factor levels in healthy pregnant women
Felice Petraglia, Alessandro D. Genazzani, Lorenzo Aguzzoli, Andrea Gallinelli, Davide de Vita, Alessandro Caruso, Andrea R. Genazzani
Neuro Ophthalmology, 1992
