Abdulsamie Hassan Hammood Alta'ee
@uobabylon.edu.iq
University of Babylon/ College of Medicine/ Department of Biochemistry
University of Babylon
RESEARCH INTERESTS
Biochemistry, Clinical chemistry
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Mahmoud Hussein Hadwan, Ahed Kamil Rahi, Esraa Rafied Abass, Asad M. Hadwan, Rawa M. Mohammed, Abdulsamie Hassan Alta’ee, Abdul Razzaq Alsalman, Muntadher M. Hadwan, and Zainab Abbas Al-Talebi
Springer Science and Business Media LLC
Background: Severe Myocardial Infarction is a highly stark manifestation of heart illness, often accompanied with additional complications such as diabetes mellitus (DM) and hypertension (HTN). The hormone within the body enables glucose to enter the cells and burn them up as fuel. HTN is a common vascular disorder that represents a major cardiovascular condition. Methods: The study was conducted at the Biochemistry Department of the College of Medicine from April 2022 to May 2023 and used the Human Vit K2 ELISA Kit, Human Lp(a) ELISA Kit, and Human N-terminal ProBST ELISA Kit to estimate the serum levels of vitamin K2, lipoprotein (a) (Lp(a)), and N-terminal of the Pro brain natriuretic peptide (NT-proBNP). Results: The results showed a important decrease in VK2 levels in AMI patients with DM and HTN than the other two groups (less than AMI patients group with and without HDL-c). On the other hand, Lp(a), and NT-pro BNP levels were significantly increased in AMI patients with DM and HTN than the other two groups (less than AMI patients group with no DM and HTN). As the diagnostic accuracy of tests increased, the area under the ROC curve for NTproBNP for diagnosing AMI achieved the highest 0.988. NTproBNP showed a promising ability to differentiate AMI patients from DM+ HTN group with 0.971 (AUC). Conclusions: In diabetic hypertensive patients with acute myocardial infarction, a low level of vitamin K2 and a high level of lipoprotein(a) might be risk markers for the onset of myocardial infarction.
Rawa Majid, Zainab Abbas Al Talebi, Hawraa Saad Al-Kawaz, Abdulsamie Hassan Alta'ee, Abdul Razzaq S. Alsalman, Asad M. Hadwan, Muntadhar M. Hadwan, and Mahmoud Hussein Hadwan
Elsevier BV
Hind Shawqi Zaki, Abdulsamie Hassan Alta’ee, and Mushtaq Qahtan Mohammed
Medknow
Abstract Background: Breast cancer (BC) is an unchecked proliferation of epithelial cells that begin in the breast lobules or ducts. BC develops and spreads as a result of the high mobility group protein box 1 (HMGB1). The survival, development, and metastasis of tumor cells have all been analyzed for the patients from Oncology Center in Merjan Medical City, Babylon Governorate. HMGB1 and receptor for advanced glycation end products (RAGE) levels in patients and controls were assessed using the enzyme-linked immunosorbent assay technique. Objectives: The current study’s goal is to analyze the blood levels of HMGB1 and RAGE in both BC patients and healthy volunteers and evaluate how their expression changes as the disease progresses. Materials and Methods: Samples collected from BC levels exhibited a 76% sensitivity and a 70% specificity, respectively. Serum RAGE levels were 74% sensitive and 70% specific for the diagnosis of BC, respectively, and their substantial P value = 0.023 correlated with tumor size. Results: Patients had significantly higher HMGB1 and RAGE levels than did the healthy control group. In order to identify BC, serum HMGB1 is linked to HMGB1 binding to the RAGE receptor. Conclusion: The presence of HMGB1 in the serum may serve as a helpful biomarker for the detection of BC. BC RAGE is useful for monitoring the growth of tumor size.
Lamia A. Almashhedy, Hussein A. Fadhil, Abdul Razzaq S. Alsalman, Hawraa Saad Al-Kawaz, Abdulsamie Hassan Alta'ee, Alaa Tariq Al-Hassnawi, Asad M. Hadwan, and Mahmoud Hussein Hadwan
Elsevier BV
AbdulsamieH Alta’ee, KhawlaA Shemran, and SarahH Edin
Medknow
Background: Hyperlipidemia is an umbrella term for any of the genetic or acquired disorders that result in an elevated level of lipids circulating in the blood. These lipids can enter the walls of arteries and increase the risk of developing hardening of the arteries, which can lead to atherosclerosis, stroke, and heart attack. Objectives: The present study tries to investigate the impact of lipid-lowering drugs on lipid profile in patients with hyperlipidemia and to determine the best drug of choice in such patients. Materials and Methods: Sixty hyperlipidemia patients with the mean age of 45.52 ± 13.24 years admitted to a private clinic in Hilla city, Iraq, during a period extant from October 2022 to November 2022 were subjected to the present cross-sectional study. Patients were categorized according to the type of drug used and gender. Group 1 (G1) patients treated with rosuvastatin, group 2 (G2) patients treated with atorvastatin, group 3 (G3) patients treated with ezetimibe, and group 4 (G4) patients treated with combination of ezetimibe + simvastatin. Lipid profile was determined using enzymatic method. Results: The combination of ezetimibe + simvastatin has a better effect to lower the body mass index. Ezetimibe alone reduces total cholesterol (TC), whereas combination of ezetimibe + simvastatin was found to reduce TC, low-density lipoprotein cholesterol (LDL-C) and triacylglycerol (TG). Rosuvastatin raises the high-density lipoprotein cholesterol (HDL-C). Conclusions: The combination of ezetimibe and simvastatin gives a good result in reducing the level of TC in the body, and this leads to a better reduction of LDL-cholesterol than using atorvastatin alone.
Aymen Abdul Rasool Jawad, Zainab Abbas Al Talebi, Abdulsamie Hassan Alta’ee, Asad M. Hadwan, Muntadher Ahmed Abdulmahdi, Mohammed A. Kadhum, Hassan Hadi Khalifa, Hawraa Saad Al-Kawaz, and Mahmoud Hussein Hadwan
Springer Science and Business Media LLC
Zainab Abbas Al Talebi, Hawraa Saad Al-Kawaz, Rasha Kadhim Mahdi, Alaa Tariq Al-Hassnawi, Abdulsamie Hassan Alta'ee, Asad M. Hadwan, Dunia Abbas khudhair, and Mahmoud Hussein Hadwan
Elsevier BV
Zainab Tamimi, Abdulsamie ee, and Ahmed Jasim
ScopeMed
Background:The most current threat to global health is the continuous spread of a respiratory disease known as COVID-19 Disease 2019 in recent years. COVID-19 was recognized in December 2019. It was quickly determined that a novel COVID-19 virus, which is structurally linked to the virus that causes the severe acute respiratory syndrome, was to cause (SARS). Objective: The aim of this study is to investigate the presence of effect between the rs179008 (A/T) SNP polymorphism in TLR7 gene and blood group on the severity of COVID-19. Methods: The study included 90 patients divided into three groups mild, moderate, severe, and experimental research work was conducted during the period of sample collection extended from November 2021 to February, PCR-RFLP technique was used to determine SNP rs179008 polymorphism in TLR7 in the blood. Results: A present study found non-significant differences between patient groups for TLR7 rs179008 (A, T) allele were (p=0.79152) for mild to moderate and severe, (p=0.84872) for mild and moderate and (p=0.58741) for mild and severe. When comparison (AA, AT, TT) genotypes in three groups found a significant difference between mild and moderate groups (p=0.036) for the AA genotype. Found (A blood group) more frequency than other groups but observes no significant difference between patients’ group. Conclusion: We conclude that the (AA) genotype for TLR7 rs179008 polymorphism was a risk factor and effect on severity of COVID-19 infection, so (AA) can consider an independent risk factor for development of COVID-19.
Ali Anbari, Abdulsamie ee, and Shokry Saad
ScopeMed
Myocardial necrosis caused by ischemia is called a myocardial infarction (MI). which interrupts coronary blood supply. When the oxygen supply to the heart is insufficient to meet metabolic demands, myocardial ischemia occurs. Atherosclerosis, which obstructs the coronary arteries, is the most common underlying cause of myocardial ischemia. The role of arginase-1 (ARG-1) and serum lipids in the pathogenesis of myocardial infarction is becoming clearer. This study aims to see if there is a link between ARG-1 activity and MI in the Iraqi population. Between the first of November 2021 and the first of February 2022, 90 people were separated into two groups: 45 patients with MI and 45 healthy controls. Human ARG-1 was measured in serum blood using the ELISA method. The serum lipid was measured using the spectrophotometry technique. The current investigation discovered a substantial (p=0.01) rise in ARG-1 concentration compared to control groups, as well as a significant difference in blood lipid content between patients and control groups (p<0.05). Finally, ARG-1 may have a role to play role in the pathogenesis of MI.
Mahmoud H. Hadwan, AbdulRazzaq S. Alsalman, Lamia A. Almashhedy, Abdulsamie H. Altaee, and Asad M. Hadwan
Springer Science and Business Media LLC
Sulfhydryl oxidase was studied using a spectrofluorometric assay. The current protocol operates by using a combination of hemoglobin (HB) and hematin (HT) as a peroxidase mimic to catalyze the H2O2-dependent oxidation of thiamine. The response surface methodology (RSM) is used to optimize the new method. The current method is very accurate, sensitive, and linear up to 200 IU. When compared to the colorimetric method, the method produced a satisfactory correlation. The novel protocol is being used to evaluate asthenospermic patients' and fertile men's seminal sulfhydryl oxidase activity. The current protocol was used to determine reference values for seminal sulfhydryl oxidase activity. Due to the fact the newly developed spectrofluorometric method is more sensitive and precise than other colorimetric methods, and because thiamine is less expensive than other types of probes used in colorimetric and spectrofluorometric methods, it is likely to find widespread use among scientists studying sulfhydryl oxidase activity in biological tissues. The present method's analytical recovery yielded high specific findings.
A. Alsalman, L. A. Almashhedy, A. Alta'ee and M. H. Hadwan
Background Uric acid (UA) is crucial for sperm metabolism as it protects seminal plasma against oxidative dam- age. Zinc also plays a central role in sperm metabolism. The current study was designed to investigate the role of zinc supplementation on qualitative and quantitative properties of seminal fluid, in parallel with the UA level and urate pathway enzymes in the semen of patients with asthenozoospermia. Materials and Methods The study was designed as a randomized controlled trial of 60 asthenozoospermic subfertile men. The current study, which was conducted during one year, involved 60 fertile and 60 asthenozoospermic subfertile men belonging to Hilla City, Iraq. Semen samples were obtained from the participants before and after treatment with zinc supplements. The levels of UA, xanthine oxidase (XO), adenosine deaminase (ADA) and 5'-nucleotidase (5'-NU) activities were determined in spermatozoa and seminal plasma of both groups. Results UA levels (P=0.034) and 5'-NU activity (P=0.046) were significantly lower but ADA (P=0.05) and XO (P=0.015) activities were significantly higher in infertile men than in healthy men. Treatment with zinc sulfate induced an increase in UA (P=0.001) level and 5'-NU activity (P=0.001), but a decrease in ADA (P=0.016) and XO (P=0.05) activities. Conclusion Zinc supplementation restores UA levels and the activities of enzymes involved in the urate pathway (XO and ADA) in the seminal plasma and spermatozoa of patients with asthenozoospermia, to reference values. Sup- plementation of Zn compounds enhances the qualitative and quantitative properties of semen (Registration number: NCT03361618).
AbdulsamieHassan Alta'ee, SarahIsam Al-Rubaye, and ZenaSaeed Al-Fadhily
Medknow
Background: Alopecia areata (AA) is an autoimmune, dermatological, chronic, inflammatory disease that attacks hair follicles and causes hair loss. Hair loss usually occurs on the scalp, but it can also affect the beard, eyebrows, and other areas of the body. Interleukin-2 (IL-2) is a cytokine that contributes to the regulation of the immune system and is classified as a proinflammatory factor. IL-2 is an autocrine secretary element produced from activated T-cells, exhibiting growth factor characteristics. Objective: The objective of this study was to investigate the effect of the −330 IL-2 gene polymorphism (rs2069762) on plasma IL-2 levels in Iraqi patients with AA. Materials and Methods: In this study, 100 patients with AA and 100 ethnicity-, age-, and sex-matched healthy controls were selected. Blood samples of all individuals were collected in EDTA tubes. The restriction fragment length polymorphism–polymerase chain reaction method was applied to determine various alleles and genotypes in these individuals. Plasma concentration of IL-2 was measured in all the samples using human IL-2 kit. Results: The frequency of −330 G/T IL-2 genotype was higher in patients with AA compared to normal individuals. Accordingly, the plasma levels of IL-2 were significantly higher (P < 0.0090) in patients when compared to the control group. Conclusion: In case of patients with AA, the −330 G/T IL-2 genotype is associated with higher plasma levels of IL-2.
Sarah Isam Al-Rubaye, Abdulsamie Hassan Alta'ee, and Zena Saeed Al-Fadhily
Diva Enterprises Private Limited
A. Alta'ee, Hanan Abbas Majeed and M. H. Hadwan