Agricultural and Biological Sciences, Plant Science, Food Science
112
Scopus Publications
3254
Scholar Citations
33
Scholar h-index
63
Scholar i10-index
Scopus Publications
Gut microbiota-driven remodeling of fresh and oxidized edible oils revealed by integrated GC-MS and UPLC-HRMS/MS metabolomics Sherine El-Shamy, Sherien M. Bakry, Ahmed Zayed, Mohamed A. Farag Npj Science of Food, 2026 Thermal processing and storage of edible oils promote lipid oxidation, generating compounds that may affect nutritional quality and consumer safety. After ingestion, these compounds are further transformed by gut microbiota, altering their chemical fate and biological impact. The current study applied an integrated gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography–high-resolution mass spectrometry–tandem mass spectrometry (UPLC-HRMS/MS) workflow coupled with feature-based molecular networking (FBMN) and chemometric analysis to simultaneously track primary and secondary metabolites formed during oil oxidation and subsequent gut microbial metabolism in corn, sesame, and sunflower oils. Metabolite profiling enabled the annotation of 89 primary metabolites by GC–MS and 55 secondary metabolites by UPLC-HRMS/MS-FBMN. Gut microbiota incubation markedly reduced several oxidation-related compounds, including 2,4-decadienal (0.05–0.11%), 2,4-nonadienal (0.01–0.25%), N -nitrosodiethanolamine (0.03–0.05%), 3,5-diethyl-2-methylpyrazine (0.02–0.24%), oxalic acid (1.2–1.8%), and diethylene glycol (0.2–0.4%), compared with uninoculated controls. In contrast, microbial incubation increased phenol (39–46%) and indole (18.2–22.6%), indicating active microbial metabolism of aromatic amino acids. These findings demonstrate how oxidation-derived oil metabolites are dynamically reshaped by human gut microbiota using a unified multi-platform metabolomics strategy, providing insight into the post-ingestion chemical fate of thermally processed edible oils.
Fucoidan-active enzymes: Recent progress in their heterologous expression and future prospects for applications Shahd Abdelrahman, Reem Zahran, Ahmed Zayed Journal of Applied Phycology, 2026 Fucoidans, also referred to as fucose-containing polysaccharides (FCPs), are structurally diverse sulfated polysaccharides predominantly found in brown seaweeds and certain marine invertebrates. They are widely recognized for their broad therapeutic and industrial potential. The bioactivities of these FCPs are highly dependent on their molecular weight distribution, degree and pattern of sulfation, and backbone architecture, representing a broad spectrum of related structures that can be selectively modified through enzymatic pathways. This review consolidates recent progress on fucoidan-active enzymes, including fucosyltransferases, sulfotransferases, fucoidanases, sulfatases, and fucosidases, focusing on their catalytic roles in fucoidan biosynthesis and degradation, as well as strategies to overcome challenges in their recombinant expression. Current efforts in bacterial and yeast hosts are critically evaluated, highlighting metabolic engineering to enhance GDP-l-fucose supply through targeted gene deletions and improved NADPH regeneration, alongside expression optimization via codon engineering, promoter tuning, and solubility-enhancing fusion tags. Advances in strain engineering (e.g., Escherichia coli SHuffle, Origami B, and yeast systems), chaperone-assisted folding, and extracellular secretion are assessed for their impact on protein solubility, stability, and functional activity. Fucoidanases and fucosidases are discussed with respect to vector selection, culture conditions, and strategies to mitigate aggregation and inclusion body formation. By bridging enzymology with protein engineering, this review provides an integrated overview of promising methods and persistent bottlenecks in the production of active, fucoidan-modifying enzymes. Enzymatic approaches are highlighted as sustainable and selective tools for producing low-molecular-weight fucoidans and defined fucooligosaccharides, providing insights into future efforts in marine glycoengineering, bioprocessing, and the development of bioactive fucoidan derivatives.
Exploring natural colorants from plants to insects: chemistry, functions, and modern uses Abeer H. Elmaidomy, Ahmed Zayed, Ghada M. Abbas, Noha H. Badr, Gerhard Bringmann, et al. Rsc Advances, 2026 Natural pigments and dyes provide a rich interface between biodiversity and chemistry, offering structurally diverse molecules with distinctive reactivity and bioorganic relevance.
Psychoactive plants and phytochemicals Mohamed A. Salem, Ahmed Zayed, Shahira M. Ezzat Phytochemistry the Military and Health Phytotoxins and Natural Defenses, 2021
Plants against malarial and typhoid fever Shahira M. Ezzat, Mohamed A. Salem, Ahmed Zayed Phytochemistry the Military and Health Phytotoxins and Natural Defenses, 2021
Identification and analysis of toxic phytochemicals Mohamed A. Salem, Ahmed Serag, Hesham R. El-Seedi, Dalia I. Hamdan, Shahira M. Ezzat, et al. Phytochemistry the Military and Health Phytotoxins and Natural Defenses, 2021
Phytosedatives for Drug Discovery Shahira M. Ezzat, Ahmed Zayed, Mohamed A. Salem Poisonous Plants and Phytochemicals in Drug Discovery, 2020
Herbal cosmeticology Shahira M. Ezzat, Mahitab H. El Bishbishy, Dina M. El Kersh, Ahmed Zayed, Mohamed A. Salem, et al. Preparation of Phytopharmaceuticals for the Management of Disorders the Development of Nutraceuticals and Traditional Medicine, 2020
Untargeted metabolomics, lipidomics, and GC–MS profiling reveal species-specific and culture-induced metabolic signatures in brown macroalgae RM Ibrahim, S Alseekh, J Haffelder, NS Ramadan, GM Abbas, AR Fernie, ... Algal Research, 104748 , 2026 2026
Gut microbiota-driven remodeling of fresh and oxidized edible oils revealed by integrated GC-MS and UPLC-HRMS/MS metabolomics S El-Shamy, SM Bakry, A Zayed, MA Farag npj Science of Food 10 (1), 144 , 2026 2026
Fucoidan-active enzymes: Recent progress in their heterologous expression and future prospects for applications S Abdelrahman, R Zahran, A Zayed Journal of Applied Phycology, 1-30 , 2026 2026
Unraveling elicitor responses in plant cell cultures through multi-platform metabolomics: biotechnological implications for phytochemical enhancement MH Baky, MB Abouelela, MA Farag, R Ulber, A Zayed Plant Cell, Tissue and Organ Culture (PCTOC) 165 (1), 18 , 2026 2026
Polysaccharide-based molecularly imprinted polymers: Design, synthesis, sustainability, characterization, and applications AA Abdella, A Zayed Carbohydrate Polymers, 124889 , 2026 2026 Citations: 3
Optimization of phenolics extraction and isolation from gymnosperms growing in Egypt using response surface methodology: Dioon edule Lindl leaves as a case study AM Akar, NM Hegazi, A Zayed, SM Ibrahim RSC advances 16 (18), 16290-16309 , 2026 2026
Exploring natural colorants from plants to insects: chemistry, functions, and modern uses AH Elmaidomy, A Zayed, GM Abbas, NH Badr, G Bringmann, J Wu, ... RSC advances 16 (8), 7097-7119 , 2026 2026
Network pharmacology and molecular docking reveal antiviral mechanisms of silver nanoparticles synthesized by Oscillatoria sp. against HCV pathogenesis L Azmy, E Al-Olayan, MAA Abdelhamid, IBM Ibraheem, A Zayed, ... Scientific Reports , 2025 2025 Citations: 1
Phytochemical composition and cytotoxic potential of Red Sea macroalgae from Egypt AF Fakhri, SM Ibrahim, N Shouaib, A Zayed Egyptian Journal of Phycology 26 (1), 90-100 , 2025 2025
Metabolomic and biofunctional insights into lemon-ginger mixture protection against heated oil hepatotoxicity NS Ramadan, A Zayed, MM El-Sayed, TA Mohamed, RS Mohamed, ... Food Research International, 117936 , 2025 2025 Citations: 1
Green synthesis of relevant and sustainable bio-applications of few-layer graphene: a multi-faceted review and future perspectives RM El-Shabasy, A Zayed, MA Farag, KR Shouir Materials Today Sustainability, 101259 , 2025 2025 Citations: 3
Tannin in foods: Classification, dietary sources, and processing strategies to minimize anti-nutrient effects A Zayed, S Abdelkareem, N Talaat, DA Dayem, MA Farag Food and Bioprocess Technology 18 (11), 9221-9249 , 2025 2025 Citations: 26
Citrus-Derived Prebiotics: Influence on Gut Microbiota Composition, Fermentation Metabolites, and Functional Health Effects A Zayed, GM Adly, L Khaled, MS Abd El-Al, MA Farag Food Bioscience, 107535 , 2025 2025 Citations: 3
Sustainable valorization of olive by-products: Environmental impact, biotechnological and probiotic approaches for bioactive recovery in alignment with circular bioeconomy MH Baky, HA Zahran, I Khalifa, EK Nabih, A Zayed, E Capanoglu, ... Critical Reviews in Food Science and Nutrition, 1-25 , 2025 2025 Citations: 4
Integrated metabolomic profiling of olive oil, olive mill wastewater, and pomace from Egyptian cultivars based on UHPLC-MS/MS and NMR coupled with chemometrics HA Zahran, S Fayez, A Zayed, MA Azab, NM Fayek, L Zhang, ... Journal of Advanced Research , 2025 2025 Citations: 5
Marine polysaccharides as sustainable precursors for carbon dots and metal nanoparticles: Preparation and biomedical applications RM El-Shabasy, AA Abdella, A Zayed Bioresource Technology, 133227 , 2025 2025 Citations: 8
β-Galactosidase inhibition explored by biochemical methods and in silico studies for plant polyphenols A Zayed, K Sayah, K Sharma, RA Radwan, SM Ezzat Archives of Biochemistry and Biophysics, 110568 , 2025 2025 Citations: 1
NMR-based metabolomics for unraveling oleo-gum resin metabolome of rare Commiphora species: insights into taxonomic variation and tree sex differences TA Mohamed, A Zayed, EA AlSherif, MA Farag, HM Abdallah Journal of Natural Medicines 79 (4), 807-820 , 2025 2025 Citations: 3
Heterologous expression of algal fucosyltransferases and sulfotransferases in Escherichia coli A Zayed, B Ledermann, T Fischöder, L Elling, N Frankenberg-Dinkel, ... Biochemical Engineering Journal 218, 109690 , 2025 2025 Citations: 2
GC-MS based nutritional and aroma profiling of date palm seeds collected from different Egyptian cultivars for valorization purposes WM Ismail, A Zayed, NS Ramadan, ST Sakna, MA Farag Scientific Reports 15 (1), 16531 , 2025 2025 Citations: 9
MOST CITED SCHOLAR PUBLICATIONS
Fucoidan characterization: Determination of purity and physicochemical and chemical properties A Zayed, M El-Aasr, ARS Ibrahim, R Ulber Marine Drugs 18 (11), 571 , 2020 2020 Citations: 242
Insights into Eucalyptus genus chemical constituents, biological activities and health-promoting effects B Salehi, J Sharifi-Rad, C Quispe, H Llaique, M Villalobos, A Smeriglio, ... Trends in Food Science & Technology 91, 609-624 , 2019 2019 Citations: 199
Valorization and extraction optimization of Citrus seeds for food and functional food applications A Zayed, MT Badawy, MA Farag Food Chemistry 355, 129609 , 2021 2021 Citations: 159
Fucoidan production: Approval key challenges and opportunities A Zayed, R Ulber Carbohydrate Polymers 211 (May 2019), 289–297 , 2019 2019 Citations: 119
Physicochemical and Biological Characterization of Fucoidan from Fucus vesiculosus Purified by Dye Affinity Chromatography A Zayed, K Muffler, T Hahn, S Rupp, D Finkelmeier, A Burger-Kentischer, ... Marine drugs 14 (4), 79 , 2016 2016 Citations: 117
Antimicrobial potentials of natural products against multidrug resistance pathogens: A comprehensive review AH Elmaidomy, NH Shady, KM Abdeljawad, MB Elzamkan, HH Helmy, ... RSC advances 12 (45), 29078-29102 , 2022 2022 Citations: 115
Fucoidan in pharmaceutical formulations: a comprehensive review for smart drug delivery systems YA Haggag, AA Abd Elrahman, R Ulber, A Zayed Marine Drugs 21 (2), 112 , 2023 2023 Citations: 114
Fucoidans: Downstream processes and recent applications A Zayed, R Ulber Marine Drugs 18 (3), 170 , 2020 2020 Citations: 111
Cynara cardunculus L.: Outgoing and potential trends of phytochemical, industrial, nutritive and medicinal merits A Zayed, A Serag, MA Farag Journal of Functional Foods 69, 103937 , 2020 2020 Citations: 107
Gut homeostasis and microbiota under attack: Impact of the different types of food contaminants on gut health MM Elmassry, A Zayed, MA Farag Critical reviews in food science and nutrition 62 (3), 738-763 , 2022 2022 Citations: 93
Mechanistic Wound Healing and Antioxidant Potential of Moringa oleifera Seeds Extract Supported by Metabolic Profiling, In Silico Network Design, Molecular … NH Shady, NM Mostafa, S Fayez, IM Abdel-Rahman, SA Maher, A Zayed, ... Antioxidants 11 (9), 1743 , 2022 2022 Citations: 69
Metabolomics-based approach for coffee beverage improvement in the context of processing, brewing methods, and quality attributes MA Farag, A Zayed, IE Sallam, A Abdelwareth, LA Wessjohann Foods 11 (6), 864 , 2022 2022 Citations: 64
Valorization, extraction optimization and technology advancements of artichoke biowastes: Food and non-food applications A Zayed, MA Farag Lwt 132, 109883 , 2020 2020 Citations: 64
Fucoidan’s molecular targets: A comprehensive review of its unique and multiple targets accounting for promising bioactivities supported by in silico studies A Zayed, DA Al-Saedi, EO Mensah, ON Kanwugu, P Adadi, R Ulber Marine Drugs 22 (1), 29 , 2023 2023 Citations: 62
Fucoidan mitigates gastric ulcer injury through managing inflammation, oxidative stress, and NLRP3-mediated pyroptosis HM Selim, WA Negm, MF Hawwal, IA Hussein, E Elekhnawy, R Ulber, ... International immunopharmacology 120, 110335 , 2023 2023 Citations: 61
Chemometrics-based aroma profiling for revealing origin, roasting indices, and brewing method in coffee seeds and its commercial blends in the Middle East A Abdelwareth, A Zayed, MA Farag Food Chemistry 349, 129162 , 2021 2021 Citations: 59
Wound-Healing Potential of Rhoifolin-Rich Fraction Isolated from Sanguisorba officinalis Roots Supported by Enhancing Re-Epithelization, Angiogenesis, Anti … WA Negm, AH El-Kadem, E Elekhnawy, NGM Attallah, GA Al-Hamoud, ... Pharmaceuticals 15 (2), 178 , 2022 2022 Citations: 54
How does LC/MS compare to UV in coffee authentication and determination of antioxidant effects? Brazilian and Middle Eastern coffee as case studies EA El-Hawary, A Zayed, A Laub, LV Modolo, L Wessjohann, MA Farag Antioxidants 11 (1), 131 , 2022 2022 Citations: 53
Dye affinity chromatography for fast and simple purification of fucoidan from marine brown algae T Hahn, A Zayed, M Kovacheva, R Stadtmüller, S Lang, K Muffler, R Ulber Engineering in Life Sciences 16 (1), 78-87 , 2016 2016 Citations: 50
Cationic dye for the specific determination of sulfated polysaccharides T Hahn, M Schulz, R Stadtmüller, A Zayed, K Muffler, S Lang, R Ulber Analytical Letters 49 (12), 1948-1962 , 2016 2016 Citations: 49
