I have completed my Bachelor's degree (B. Pharm) in the year 2018 from Gupta College of Technological Sciences with a YGPA of 9.62, affiliated with the Maulana Abul Kalam Azad University of Technology, West Bengal. I have completed my Master's (M. Pharm) in the year 2020 from Jamia Hamdard with CGPA 8.8. Currently, I am working as a Ph.D. research scholar in the department of pharmaceutical engineering and technology, the Indian Institute of Technology, BHU, Varanasi.
RESEARCH INTERESTS
The research interests comprise the development of nanoparticles and diagnostic tools for the effective treatment of various types of cancer
35
Scopus Publications
1082
Scholar Citations
19
Scholar h-index
26
Scholar i10-index
Scopus Publications
Design and Synthesis of S-Acetophenylhydrazones of 5-Methyl-1,3,4-Thiadiazole-2-Thiol as SHP2 Inhibitory Agents Rangan Mitra, Sandeep Kumar, Aiswarya Chaudhuri, Shreyasi Majumdar, Sairam Krishnamurthy, et al. Chemistry and Biodiversity, 2025 We report herein the rational design, synthesis and evaluation of a library of 21 S‐acetophenylhydrazones of 5‐methyl‐1,3,4‐thiadiazole‐2‐thiol bearing a substituted benzylidene moiety (compounds 5a–5u). Compound 5f ((E)‐N'‐(2,6‐dichlorobenzylidene)‐2‐((5‐methyl‐1,3,4‐thiadiazol‐2‐yl)thio)acetohydrazide) emerged as the most potent SHP2 inhibitor (IC50 = 0.120 ± 0.006 µM). Further, cell‐based studies revealed its moderate yet best‐in‐library cytotoxicity against MCF‐7 cells (IC50 = 86.46 ± 2.32 µM) in a dose‐dependent manner. Compound 5f also exhibited in vitro anti‐survival (33% colony formation) and anti‐migratory effect (45% wound closure) on MCF‐7 cells upon treatment with sub‐IC50 dose. In vivo toxicological evaluation of compound 5f revealed excellent safety profile in mice at an oral dose of 2000 mg/kg BW. Our current study yielded a novel heterocyclic scaffold designed through systematic lead‐guided optimization displaying submicromolar SHP2 inhibitory activity. The lead molecule identified from in vitro studies was effective against the proliferation, survival and migration of human breast cancer cells with an excellent in vivo safety profile.
In vitro anticancer effects of frankincense and its nanoemulsions for enhanced cancer cell targeting Rayya A. Al-Balushi, Aiswarya Chaudhuri, Raghuram Kandimalla, Ashanul Haque, Khalaf M. Alenezi, et al. Frontiers in Pharmacology, 2025 IntroductionFrankincense has demonstrated promising in vitro anticancer activity. However, its conventional delivery methods face significant challenges due to limited oral bioavailability. To address these limitations, this study focuses on developing optimized nanoemulsions (NEs) of Frankincense oil (FO) to enhance its therapeutic efficacy.MethodsFrankincense resins were extracted and characterized using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), identifying key metabolites including isopinocarveol, α-thujene, p-cymene, carvone, germacrene A, and various methyl esters. FO-based nanoemulsions (FO-NEs) were prepared and optimized using a 3-factor, 3-level Box-Behnken Design (BBD), with 10% FO (v/v), 40% surfactant (cremophor EL), and co-surfactant (Transcutol P). The optimized FO-NEs were evaluated for particle size, polydispersity index (PDI), zeta potential, and morphology using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Cytotoxicity, wound healing, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) assays were performed against breast cancer (MDA-MB-231, MDA-MB-231-TR) and lung cancer (A549, A549-TR, H1299) cell lines.ResultsThe optimized FO-NEs exhibited an average particle size of 65.1 ± 4.21 nm, a PDI of 0.258 ± 0.04, and a zeta potential of −22.3 ± 1.2 mV. SEM and AFM confirmed the spherical morphology of the FO-NEs. In vitro cytotoxicity studies revealed enhanced anticancer activity of FO-NEs (IC50 = 13.2 μg/mL) compared to free FO (IC50 = 22.5 μg/mL) against resistant breast cancer MDA-MB-231-TR cells. FO-NEs significantly improved cancer cell internalization, disrupted mitochondrial membrane potential, and increased ROS generation, leading to enhanced cytotoxic effects.DiscussionThe results demonstrate that nanoemulsion-based delivery significantly enhances the bioactivity and cellular uptake of frankincense oil compared to its free form. FO-NEs exhibit potent anticancer activity, particularly against drug-resistant cancer cell lines, suggesting their potential as a viable strategy for improving the therapeutic efficacy of frankincense in cancer treatment.
An Investigation of In Vitro Anti-Cancer Efficacy of Dihydroartemisinin-Loaded Bovine Milk Exosomes Against Triple-Negative Breast Cancer Dulla Naveen Kumar, Aiswarya Chaudhuri, Udita Shiromani, Dinesh Kumar, Ashish Kumar Agrawal AAPS Journal, 2024 Repurposing drugs offers several advantages, including reduced time and cost compared to developing new drugs from scratch. It leverages existing knowledge about drug safety, dosage, and pharmacokinetics, expediting the process of clinical trials and regulatory approval. Dihydroartemisinin (DHA) is a semi-synthetic and active metabolite of all artemisinin molecules and is FDA-approved for the treatment of malaria. Apart from having anti-malarial properties, DHA also possesses anticancer properties. However, its pharmacological actions are limited by toxicity and solubility problems. To overcome these challenges and enhance its anticancer effectiveness, we designed an exosomal formulation of DHA. We isolated exosomes from bovine milk using differential ultracentrifugation and loaded DHA using sonication. Scanning and transition electron microscopy revealed a size of roughly 100 nm, with a spherical shape. Furthermore, in pH 7.4 and 5.5, the exosomes exhibited burst release followed by sustained release. Multiple in vitro cell culture tests demonstrated that Exo-DHA exhibited enhanced anticancer activity, including cytotoxicity, cellular uptake, generation of reactive oxygen species (ROS), disruption of mitochondrial membrane potential, and inhibition of colony formation. Additional evidence supporting Exo-DHA's anti-migration ability came from transwell migration and scratch assays. Based on these results, it was concluded that the anticancer efficacy of DHA was improved when loaded into bovine milk-derived exosomes. While the in vitro results are encouraging, more in vivo testing in suitable animal models and biochemical marker analysis are warranted.
Azacoumarin-based “turn-on” fluorescent probe for the detection and imaging of hydrogen peroxide in living cells Samarpita Das, Aiswarya Chaudhuri, Harish K. Indurthi, Ashish K. Agrawal, Deepak K. Sharma Organic and Biomolecular Chemistry, 2024 Herein, we demonstrate azacoumarins as potential fluorophores and their application in the synthesis of a “turn-on” fluorescent probe (PYCB) for the detection of H2O2 using a carboxy benzyl boronic pinacol ester as the peroxide recognition unit.
Design and Synthesis of S ‐Acetophenylhydrazones of 5‐Methyl‐1,3,4‐Thiadiazole‐2‐Thiol as SHP2 Inhibitory Agents R Mitra, S Kumar, A Chaudhuri, S Majumdar, S Krishnamurthy, ... Chemistry & Biodiversity 22 (9), e202402997 , 2025 2025
Oral delivery of dihydroartemisinin for the treatment of melanoma via bovine milk exosomes DN Kumar, A Chaudhuri, D Dehari, AM Gamper, D Kumar, AK Agrawal Drug Delivery and Translational Research 15 (8), 2862-2877 , 2025 2025 Citations: 12
Exploring combinations of dihydroartemisinin for cancer therapy: A comprehensive review K Rani, RC Sahu, A Chaudhuri, DN Kumar, S Arora, D Kumar, ... Biochemical and Biophysical Research Communications 765, 151854 , 2025 2025 Citations: 11
In vitro anticancer effects of frankincense and its nanoemulsions for enhanced cancer cell targeting RA Al-Balushi, A Chaudhuri, R Kandimalla, A Haque, KM Alenezi, ... Frontiers in Pharmacology 16, 1403780 , 2025 2025 Citations: 5
Non-Traditional Antibacterial Therapeutic: Focus on Safety, Manufacturing, and Regulatory Dynamics D Dehari, A Chaudhuri, DN Kumar, AK Agrawal Emerging Paradigms for Antibiotic-Resistant Infections: Beyond the Pill, 787-810 , 2024 2024 Citations: 4
Corrigendum to “Exosomes improved the in vitro anti-melanoma efficacy of dihydroartemisinin”[J. Drug Delivery Sci. Tech. 9 (2024) 105957] DN Kumar, A Chaudhuri, D Dehari, AM Gamper, D Kumar, AK Agrawal Journal of Drug Delivery Science and Technology 99, 105993 , 2024 2024
Exosomes improved the in vitro anti-melanoma efficacy of dihydroartemisinin DN Kumar, A Chaudhuri, D Dehari, AM Gamper, D Kumar, AK Agrawal Journal of Drug Delivery Science and Technology 99, 105957 , 2024 2024 Citations: 4
An Investigation of In Vitro Anti-Cancer Efficacy of Dihydroartemisinin-Loaded Bovine Milk Exosomes Against Triple-Negative Breast Cancer DN Kumar, A Chaudhuri, U Shiromani, D Kumar, AK Agrawal The AAPS journal 26 (5), 91 , 2024 2024 Citations: 19
Functionalized solid lipid nanoparticles combining docetaxel and erlotinib synergize the anticancer efficacy against triple-negative breast cancer A Chaudhuri, DN Kumar, D Kumar, AK Agrawal European Journal of Pharmaceutics and Biopharmaceutics 201, 114386 , 2024 2024 Citations: 15
Design, Synthesis and Evaluation of Thioacetamide‐Tethered Thiadiazole‐1, 2, 4‐Triazole Hybrids as SHP2 Inhibitors for Cancer Therapy R Mitra, S Kumar, A Chaudhuri, AK Agrawal, SR Ayyannan ChemistrySelect 9 (27), e202401602 , 2024 2024 Citations: 1
Combinatorial delivery of docetaxel-and erlotinib-loaded functionalized nanostructured lipid carriers for the treatment of triple-negative breast cancer using quality-by-design … A Chaudhuri, DN Kumar, SK Srivastava, D Kumar, UK Patil, AS Parmar, ... Pharmaceutics 16 (7), 926 , 2024 2024 Citations: 15
Enhanced therapeutic efficacy against melanoma through exosomal delivery of hesperidin DN Kumar, A Chaudhuri, D Dehari, AM Gamper, D Kumar, AK Agrawal Molecular pharmaceutics 21 (6), 3061-3076 , 2024 2024 Citations: 28
Azacoumarin-based “turn-on” fluorescent probe for the detection and imaging of hydrogen peroxide in living cells S Das, A Chaudhuri, HK Indurthi, AK Agrawal, DK Sharma Organic & Biomolecular Chemistry 22 (36), 7332-7336 , 2024 2024 Citations: 6
Bacteriophage entrapped chitosan microgel for the treatment of biofilm-mediated polybacterial infection in burn wounds D Dehari, DN Kumar, A Chaudhuri, A Kumar, R Kumar, D Kumar, S Singh, ... International Journal of Biological Macromolecules 253, 127247 , 2023 2023 Citations: 23
A bacteriophage-loaded microparticle laden topical gel for the treatment of multidrug-resistant biofilm-mediated burn wound infection D Dehari, A Chaudhuri, DN Kumar, M Anjum, R Kumar, A Kumar, ... AAPS pharmscitech 24 (6), 165 , 2023 2023 Citations: 12
Impact of the drug loading method on the drug distribution and biological efficacy of exosomes DN Kumar, A Chaudhuri, D Kumar, S Singh, AK Agrawal Aaps Pharmscitech 24 (6), 166 , 2023 2023 Citations: 50
Recent advancements in nanotechnology-based bacteriophage delivery strategies against bacterial ocular infections R Patil, D Dehari, A Chaudhuri, DN Kumar, D Kumar, S Singh, G Nath, ... Microbiological research 273, 127413 , 2023 2023 Citations: 39
A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds D Dehari, A Chaudhuri, DN Kumar, R Patil, M Gangwar, S Rastogi, ... Pharmaceuticals 16 (7), 942 , 2023 2023 Citations: 28
Endorsement of TNBC biomarkers in precision therapy by nanotechnology A Chaudhuri, DN Kumar, D Dehari, R Patil, S Singh, D Kumar, ... Cancers 15 (9), 2661 , 2023 2023 Citations: 31
Introduction to smart polymers and their application. Smart Polymeric Nano-Constructs in Drug Delivery A Chaudhuri, KK Sandha, AK Agrawal, PN Gupta Academic Press , 2023 2023 Citations: 20
MOST CITED SCHOLAR PUBLICATIONS
Docetaxel: An update on its molecular mechanisms, therapeutic trajectory and nanotechnology in the treatment of breast, lung and prostate cancer M Imran, S Saleem, A Chaudhuri, J Ali, S Baboota Journal of Drug Delivery Science and Technology 60, 101959 , 2020 2020 Citations: 171
Lipid-based nanoparticles as a pivotal delivery approach in Triple Negative Breast Cancer (TNBC) therapy A Chaudhuri, DN Kumar, RA Shaik, BG Eid, AB Abdel-Naim, S Md, ... International Journal of Molecular Sciences 23 (10068), 1-39 , 2022 2022 Citations: 90
Exosomes as emerging drug delivery and diagnostic modality for breast cancer: recent advances in isolation and application DN Kumar, A Chaudhuri, F Aqil, D Dehari, R Munagala, S Singh, ... Cancers 14 (6), 1435 , 2022 2022 Citations: 85
Polymeric micelles: A novel drug delivery system for the treatment of breast cancer A Chaudhuri, K Ramesh, DN Kumar, D Dehari, S Singh, D Kumar, ... Journal of Drug Delivery Science and Technology 77, 103886 , 2022 2022 Citations: 81
Combination therapy comprising paclitaxel and 5-fluorouracil by using folic acid functionalized bovine milk exosomes improves the therapeutic efficacy against breast cancer DN Kumar, A Chaudhuri, D Dehari, A Shekher, SC Gupta, S Majumdar, ... Life 12 (8), 1143 , 2022 2022 Citations: 79
Impact of the drug loading method on the drug distribution and biological efficacy of exosomes DN Kumar, A Chaudhuri, D Kumar, S Singh, AK Agrawal Aaps Pharmscitech 24 (6), 166 , 2023 2023 Citations: 50
Emergence of nanotechnology as a powerful cavalry against triple-negative breast cancer (TNBC) A Chaudhuri, DN Kumar, D Dehari, S Singh, P Kumar, PK Bolla, D Kumar, ... Pharmaceuticals 15 (5), 542 , 2022 2022 Citations: 48
Designing and development of omega-3 fatty acid based self-nanoemulsifying drug delivery system (SNEDDS) of docetaxel with enhanced biopharmaceutical attributes for management … A Chaudhuri, N Shrivastava, S Kumar, AK Singh, J Ali, S Baboota Journal of Drug Delivery Science and Technology 68, 103117 , 2022 2022 Citations: 41
Recent advancements in nanotechnology-based bacteriophage delivery strategies against bacterial ocular infections R Patil, D Dehari, A Chaudhuri, DN Kumar, D Kumar, S Singh, G Nath, ... Microbiological research 273, 127413 , 2023 2023 Citations: 39
Endorsement of TNBC biomarkers in precision therapy by nanotechnology A Chaudhuri, DN Kumar, D Dehari, R Patil, S Singh, D Kumar, ... Cancers 15 (9), 2661 , 2023 2023 Citations: 31
Natural, synthetic and their combinatorial nanocarriers based drug delivery system in the treatment paradigm for wound healing via dermal targeting MK Iqubal, S Saleem, A Iqubal, A Chaudhuri, FH Pottoo, J Ali, S Baboota Current Pharmaceutical Design 26 (36), 4551-4568 , 2020 2020 Citations: 31
Enhanced therapeutic efficacy against melanoma through exosomal delivery of hesperidin DN Kumar, A Chaudhuri, D Dehari, AM Gamper, D Kumar, AK Agrawal Molecular pharmaceutics 21 (6), 3061-3076 , 2024 2024 Citations: 28
A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds D Dehari, A Chaudhuri, DN Kumar, R Patil, M Gangwar, S Rastogi, ... Pharmaceuticals 16 (7), 942 , 2023 2023 Citations: 28
Nanotherapeutics in autophagy: A paradigm shift in cancer treatment S Negi, A Chaudhuri, DN Kumar, D Dehari, S Singh, AK Agrawal Drug Delivery and Translational Research 12 (11), 2589-2612 , 2022 2022 Citations: 28
Bacteriophage entrapped chitosan microgel for the treatment of biofilm-mediated polybacterial infection in burn wounds D Dehari, DN Kumar, A Chaudhuri, A Kumar, R Kumar, D Kumar, S Singh, ... International Journal of Biological Macromolecules 253, 127247 , 2023 2023 Citations: 23
Targeted delivery of natural bioactives and lipid-nanocargos against signaling pathways involved in skin cancer MK Iqubal, A Chaudhuri, A Iqubal, S Saleem, MM Gupta, A Ahuja, J Ali, ... Current Medicinal Chemistry 28 (39), 8003-8035 , 2021 2021 Citations: 22
Therapeutic potential of venom peptides: Insights in the nanoparticle-mediated venom formulations AV Joglekar, D Dehari, MM Anjum, N Dulla, A Chaudhuri, S Singh, ... Future Journal of Pharmaceutical Sciences 8 (1), 34 , 2022 2022 Citations: 21
Herbal cosmeceuticals: New opportunities in cosmetology M Aqil, A Chaudhuri, A Qadir Trends in Phytochemical Research 4 (3), 117-142 , 2020 2020 Citations: 21
Introduction to smart polymers and their application. Smart Polymeric Nano-Constructs in Drug Delivery A Chaudhuri, KK Sandha, AK Agrawal, PN Gupta Academic Press , 2023 2023 Citations: 20
An Investigation of In Vitro Anti-Cancer Efficacy of Dihydroartemisinin-Loaded Bovine Milk Exosomes Against Triple-Negative Breast Cancer DN Kumar, A Chaudhuri, U Shiromani, D Kumar, AK Agrawal The AAPS journal 26 (5), 91 , 2024 2024 Citations: 19