Mohammad Mahmoud Farhan Al-Halbosiy

@nahrainuniv.edu.iq

Biotechnology Research Center, Al-Nahrain University
Al-Nahrain University

Mohammad Mahmoud Farhan Al-Halbosiy


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https://researchid.co/al-halbosiy

RESEARCH INTERESTS

Molecular Immunology and Animal Cell Culture
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Scopus Publications

Scopus Publications

  • Evaluating the levels of dopamine and inflammatory cytokines with some biochemical variables in children with white matter atrophy in the city of Fallujah
    Ahmed Flayyih Hasan, Waleed Khalid Ahmed, Mohammed Amer Fayyadh, Mohammad Mahmoud Farhan Al-Halbosiy
    Fayoum University Medical Journal, 2026
  • Evaluation the Effect of Propofol Drug on Heart Rate During Surgery and Some Biochemical Variables Before and After Surgery
    Abdullah Abdulsattar Raeef, Hend Abass, Baida Ayada, Mohammed M. Farhan Al-Halbosiy, Thamir Ismail
    Jordan Journal of Pharmaceutical Sciences, 2026
    Background and objective: Several studies have shown that propofol drug affects intraoperative heart rate and some post and pre-operative chemical variables that affect the endocrine system. Methods: A descriptive quantitative study (15 January–15 March 2022) examined 100 adults undergoing surgery at Al Ramadi Teaching Hospital. Biochemical variables (Sodium, Potassium, Chloride, Cortisol, ACTH) and heart pulse rates during surgery were assessed for two groups: before and after surgery. The comparison between study variables were done by IBM SPSS and by using paired t-test and ANOVA test. Results: The most represented age group was 10-20 years, while the least represented was 41-50 years. Female participants constituted 60% of the sample, compared to 40% males. Normal BMI for persons undergo surgery was 30 percent from all cases, while 30 percent have overweight and 30 percent was obesity. Significant differences in cortisol, ACTH, potassium, and sodium levels were noted between the two groups (p < 0.05), with potassium and sodium levels showing a decline while cortisol and ACTH levels exhibited an increase. No significant differences were found in chloride levels (p > 0.05). The heart rates during surgery (propofol effect) were measured at first 15 minutes was 94.5 and after 30 minutes was 95.6, while pulses after 45 minutes decrease with value 86.6 and return increasing after 60 minute to value 97.4.The heart rate was significant differences in the third measurement (after 45 minutes from giving propofol) with decreasing in value of heart rate. Conclusion: Propofol drug increasing sodium and potassium concentration post-surgery, in addition, stimulates the endocrinology system to release ACTH and cortisol hormone. Propofol administration resulted in a stable heart rate initially, followed by a significant decrease to 86.6 at 45 minutes, before increasing again to 97.4 at 60 minutes.
  • Detection of LPG2 and GP63 genes sequencing in patients with cutaneous leishmaniasis
    Othman M. Muhammad, Thaer A. Salih, Mohammad M. Al-Halbosiy
    Journal of Parasitic Diseases, 2025
  • The Antileshmaniasis Effect of Brassica juncea Seeds Fatty Acids on the Growth of Leishmania tropica Promastigotes Isolated Culture in vitro
    Maksood Adil Mahmoud Al-Doori, Mohammad Mahmoud Farhan Al-Halbosiy, Zainab Yaseen Mohammed Hasan, Sarmed Mohammed Hussein, Ahmed Flayyih Hasan, et al.
    Asian Journal of Dairy and Food Research, 2025
    Background: Brassica juncea has many important phytochemical compounds like phenols, tannins, saponin, flavonoids, alkaloids, carbohydrates, proteins and important non-polar metabolites that possessed biological and nutrient importance. Methods: The study employed extraction of fatty non polar components with petroleum ether from B. juncea (mustered)seeds and applying chemical analysis of mustard seeds extract with HPLC technique to investigate the chemical composition of the oil seeds that the plant rich with. Besides; study the effect of B. juncea non-polar seeds extract on the growth of Leishmania tropica promastigotes in vitro. Result: Results showed that the mustard seeds were rich with important fatty acid such as Erucic acid, -Oleic acid and Palmatic acid as matched the retention times of the standard used in the study. These compounds had been found to be toxic for cutaneous leishmaniasis culture that, affected the parasites with prolonged treatment periods.
  • In silico characterisation of a mitotic kinesin-related protein from Leishmania donovani KE16
    Suad Gazi AL Kufi, Amjed Qays Ibrahim Alqaisi, Mohammad Mahmoud Farhan Al- Halbosiy, Ikhlass Ali Hussain AlHilaly
    Asia Pacific Journal of Molecular Biology and Biotechnology, 2025
    Mitotic kinesin proteins have a significant role in eukaryotic cell mitosis. The kinesin motor domain of Leishmania donovani has been explored as a promising vaccine candidate due to its ability to generate strong immune responses. The current study aimed to fill the knowledge gap and to systematically identify the main characteristic of kinesin related protein in L. donavani strain KE16 (LdonKE16) (AAT40474.1) using TriTrypDB (kinetoplast genomics), NCBI and EMBL database tools. The study was carried out using in silico PCR analysis of LdonKE16 as a potential of mitotic kinesin protein. Amino acid sequence of the kinesin motor domain regions was detected by using BLASTp for AAT40474.1 and showed various identities with other Leishmania spp. The highest sequence identity with L. donovani K39 (98.10%), then with L. infantum (97.09%), and (97%) with L. chagasi, and L. braziliensis respectively. While L. mexicana showed low sequence identity (61%). The conserved domains KISc of this protein contains three typical domains, the N-terminal motor domain (11 to 399 aa), the coiled segment neck domain (429 to 622aa) and the tail region (681 to 858 aa). The current study revealed that the predicted functional structure of LdonKE16 has active state and inactive state by using bioinformatics tool I-TASSER. The PCR product confirmed the identification of the kinesin protein in LdonKE16. Sequence analysis demonstrated strong homology between LdonKE16 and kinesin K39 of visceral Leishmania species, aligning with the kinesin superfamily KIF13 found in parasites and humans. These findings suggest that LdonKE16 is a promising candidate for vaccine development and therapeutic interventions against leishmaniasis.
  • Study Polymorphism of MET Gene in Cancer Iraqi Patients (AML)
    Journal of Neonatal Surgery, 2025
  • Ameliorating orthodontic relapse using laser bio-stimulation and mesenchymal stem cells in rats
    Samer S. Othman, Ali Saafan, Mohammad M.F. Al-Halbosiy, Iman Fathy, Mohamed Khursheed Alam, et al.
    Journal of Genetic Engineering and Biotechnology, 2024
  • Effect of Glycolipoprotein Biosurfactant from Enterococcus faecium on the Viability of Breast Cancer Cell Lines
    Dijlah A. Alimeer, Nibras N. Mahmood, Mohammad M. F. Al-Halbosiy
    Journal of Contemporary Medical Sciences, 2023
    Objective : This study , was research for cytotoxicity of bio-surfactant created by Enterococcus faecium isolated from feces of Iraqi healthy breast-fed infants with age < 6 months.
 Methods : Cold acetone precipitation was used to the extraction of extracellular Glycolipoprotein biosurfactant and partially purify it. Biosurfactant was then evaluated against two the cell lines, a Breast cancer MCF-7 cell line and a human normal fibroblast cell line NHF), specifically for cell survival and proliferation. 
 Results : At all concentrations with varying percentage, The viability of the MCF-7 cancer cell line was shown to be reduced with the addition of biosurfactant.; maximum inhibition percentage was 74.2% at a 100 µg/ml concentration, which is lesser than 45.5% cytotoxicity Of NHF healthy fibroblasts cell line.
 Conclusions : The findings of this study are highly encouraging in terms of the potential of Glycolipoprotein biosurfactants to treat cancer and encourage additional research with different cell lines.
  • Artemisinin Efficacy on iNOS Production in U937 Cell-line Infected with Leishmania donovani
    Suhair Dakhil Neamah, Hayder Z. Ali, Mohammad M.F. Al-Halbosiy
    Iraqi Journal of Science, 2023
    Visceral leishmaniasis (VL), the second-most-serious parasitic illness after malaria, is currently endemic in more than 88 countries. Need for new anti-leishmanial compounds is currently being taken into consideration by researchers due to resistance and lack of effective vaccinations. This research was conducted to find out more about the effect of artemisinin (ART). ART was examined in vitro promastigotes stages and ex vivo amastigotes stages of the Iraqi strain of Leishmania donovani in U937 cell line after 24, 48 and 27 hours using MTT assay. In addition, the level of macrophage nitric oxide (NO) was measured using Griess assay in U937 cell line. The results of promastigotes viability percentage were less than 50 %, where the IC50 was 43.8, 37.9 and 36.6 µM respectively. Similar cytotoxic effect of ART was observed against amastigote forms, where the cell viability was 36.11, 40.2 and 37.4 µM respectively, after the three times of follow up.. Furthermore, by using Griess assay the level of nitrite in infected macrophages was measured. The results after 48hr were 6.58 in high concentration and 1.22 in lower concentration. However, considering the absence of a significant difference, the observed concentrations are still higher than the control when compared to Griess assay.. These findings demonstrated that ART has a cytotoxic effect on both forms of L. donovani. It also increased iNOS production in infected macrophages which can define artemisinin therapeutic efficacy against L. donovani infection.
  • Extraction and purification of biosurfactant by locally isolated Enterococcus faecium bacteria and study of its antimicrobial activity
    Dijlah A. Alimeer, Nibras N. Mahmood, M. F. Al-Halbosiy
    Journal of Global Innovations in Agricultural Sciences, 2023
    In this research, the biosurfactant production by Enterococcus faecium isolated from Raw cow's milk was studied when growing in the MRS medium containing Lactose as a carbon source without Tween-80. E.faecium supernatant exhibited emulsion action of 60% towards kerosene oil as oil in water, while the surface activity was 34 mm. Biosurfactants with surface tension activity properties were extracted and partially purified from E. faecium by cold acetone 3:1, The chemistry structure of the biosurfactant was analyzed, which indicated that it is a glycol-lipo-peptide. Biosurfactant that purified from E. faecium showed antibacterial activity by reducing growth of several pathogenic G-ve and G+ve bacteria, including aureus, Staphylococcus epidermidis, Streptococcus sp., Staphylococcus Escherichia coli, Klebsiella sp., Pseudomonas aeroginosa, Acinetobacter sp. and Serratia sp. The inhibitory activity of biosurfactant against pathogenic bacteria was studied by using agar diffusion method, where the biosurfactant had higher inhibitor activity against E. coli, where highest diameter of inhibition zone was (23mm). At all concentrations examined, a biosurfactant derived from E. faecium considerably decreased bacterial adhesion, albeit to a lower amount. Keyword: Enterococcus faecium, LAB, Biosurfactant, Antibacterial and Antiadhesive activity.
  • Short Communication: Antimicrobial of lemongrass (Cymbopogon citratus L.) volatile oil and cytotoxic effects against L20B and MCF-7cell lines
    Biodiversitas, 2022
  • Anticancer and Cytotoxic Effect of Verotoxin 1 on Colon Cancer Cell Line
    Mustafa Attiyah Hadid, Mohammad M. F. Al-Halbosiy, Abdulwahid B. Al-Shaibani
    Microbiology and Biotechnology Letters, 2022
  • Effect of Cold Atmospheric Plasma on the Wound of Diabetic Rats by FE-DBD System
    Ban H. Adil, Mohammad M. F. Al-Halbosiy, Maath Salim Mohmmed, Waseem K. Kaith, Danea A. Abdalrazaq, et al.
    Aip Conference Proceedings, 2022
  • Prevalence and Cytotoxic Effects of Some Colibactin and cnf Genes among Escherichia coli Isolated from Urinary Tract Infections
    Hiba A.S. Alhadidi, Safaa A. S. Al-Qaysi, Mohammad M. F. Al-Halbosiy
    Microbiology and Biotechnology Letters, 2022
  • Detection of artemisinin effect on macrophage inducible nitric oxide gene expression in macrophage infected with Leishmania donovani
    Suhair Dakhil Neamah, H. Z. Ali, M. M. Al-halbosiy
    Annals of Parasitology, 2022
  • The Role of Nkx-6.1 Gene Expression in Diabetic and Non-diabetic Patients
    International Journal of Drug Delivery Technology, 2022
  • In Vitro Cytotoxic Activity of Total Flavonoid from Equisetum Arvense Extract
    Raghda Makia, Khulood Al-Sammarrae, Mohammad Al-Halbosiy, Mohammed Al-Mashhadani, and
    Reports of Biochemistry and Molecular Biology, 2022
  • Bioactive levan-Type exopolysaccharide produced by Pantoea agglomerans ZMR7: Characterization and optimization for enhanced production
    Safaa A. S. Al-Qaysi, Halah Al-Haideri, Sana M. Al-Shimmary, Jasim M. Abdulhameed, Othman I. Alajrawy, et al.
    Journal of Microbiology and Biotechnology, 2021
  • Cytotoxic activity of green zinc selenide nanoparticles against Hep-G2 cell lines
    Indian Journal of Forensic Medicine and Toxicology, 2021
  • Antimicrobial activity of locally synthesized carbon nanosphere on some pathogenic species of bacteria and parasites
    Baghdad Science Journal, 2021
  • Effect gold nanoparticles generated by cold plasma for mineral blood
    M. F. Al-Halbosiy, Ban H. Adil, A. S. Obaid, Maryam Kays Abdullah, Alzahraa fadhel adnan
    Aip Conference Proceedings, 2020
  • In vitro cytotoxicity study of Pt nanoparticles decorated TiO2 nanotube array
    Shaymaa Rajab Baqer, Abdulkareem Alsammarraie, Mahasin Alias, Mohammad Al-Halbosiy, Amaal Sadiq
    Baghdad Science Journal, 2020
  • Polymorphisms of TGF-BETA1 gene (+869T/C) and risk factor in Iraqi Asthma Patients
    Biochemical and Cellular Archives, 2020
  • Cloning and Expression of Truncated Haemoglobin Receptor of Leishmania tropica as a Vaccine Candidate
    May Ridha Jaafar, Yaseen Ismael Mamoori, Mohammad Mahmoud Al-Halbosiy
    Annals of Tropical Medicine and Public Health, 2020
  • Biological activities of iraqi fig (Ficuscarica) crude ethanolic and total flavonoids extracts
    Mohammad Mahmoud Farhan Al-Halbosiy, Zainab Yaseen Mohammed Hasan, Farooq Ibrahem Mohammad, Baraa Abdulhadi Abdulhameed
    Iraqi Journal of Science, 2020

Publications

●Ad'hiah,A.H.;Al-Halbosiy,M.M.F. & Al-Jumaily,R.M.Kh (2007). " Modulating the Hematological and cytogenetic Effects of Mitomycin C by Aqueous Extract of Nut Grass(Cyperus rotundus L.).J.Biotechnology Research Center 1(1):43-51.
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●Al-Halbosiy,M.M.F.;Al-JumaiJly,R.M.. (2007) ." The Mutagenic Effects of Mitomycin C by Aqueous Modulating Extract of Alhagi graecorum" Ibn Al-Hatham ,J.Pur& 20(2):1-7.
● Al-Halbosiy,M.M.F.; Sulaiman,G.M. & Al-JumaiJly,R.M.Kh. (2008)."The modulator effect of Iraqi propolis Extract on Mitomycin C Induced micronucleus formation in albino male mice". faculty, , 50(1):77-82.
● Al-Halbosiy,M.M.F. (2009)." Modulating the Mutagenic Effects of Mitomycin C by Aqueous Extract of Lentil (Lens culinars)" J.Biotechnology Research Center, 3(1):37-43.
●Al-Jumaily,R.M.Kh &; Ad'hiah,A.H. & Al-Halbosiy,M.M.F. (2010)."Evaluation of some haematological and cytogenetic effects of Ammi majus seed aqueous extract in albino male mice". Journal of Biotechnology Research Center, (special edition) Vol.4 No.1
●Hatem Tallima, Mohammad Farhan Al-Halbosiy, Rashika El Ridi(2011). Enzymatic activity and immunolocalization of Schistosoma mansoni and Schistosoma haematobium neutral
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sphingomyelinase. Molecular & Biochemical Parasitology (178): 23–28.
●Rashika El Ridi, Hatem Tallima, Mohamed Salah, Marwa Aboueldahab, Omar M. Fahmy,Mohammad Farhan Al-Halbosiy, Soheir S. Mahmoud (2012). Efficacy and mechanism of action of arachidonic acid in the treatment of hamsters infected with Schistosoma mansoni or Schistosoma haematobium. International Journal of Antimicrobial Agents (39): 232– 239.
● Mohammed M. F. Al-Halbosiy, Rakad M. Kh. Al- Jumaily, Fadhel M. Lafta & Hussam M. Hassan (2013). Inhibitory Effect of Camel Urine on Neoplastic and Transformed Cell Lines. International Journal for Sciences and Technology Vol. 8, No.2, 31-35.
●Rakad M. Kh. Al- Jumaily, Laith M.J. Al-Shamma, Mohammad M.F. Al-Halbosiy (2013). The ability of rapeseed (brassica napus I.) Seeds oil in inhibiting cell line growth in vitro. Iraqi Journal of Biotechnology, , No.1, 106-113.
● Rakad M. Kh. Al- Jumaily, Nawal MJ Al- Shamma, Mohammad M. Al-Halbosiy ,Laith M.J. Al-Shamma (2014). Anticancer Activity of Sunflower (Helianthus annuns L.) Seeds oil against cell line. Iraqi Journal of Science Vol. 54, supplement No.4, 1003-1009.
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●Zainab Y. Mohammed, Saad M. Nada, Mohammad M. Al-Halbosiy, Shaimaa Y.Abdulfattah , Baraa Abdul-Hameed (2014). Cytotoxic effects of Ammi visnaga volatile oil on some cancer cell lines. Journal of Biotechnology Research Center (special edition). Vol.8 No.1, 5-7.
●Mohammad M. F. Al-Halbosiy, Farah Th. Abdulla, Shimaa Y. Abdulfattah, Sabah M. Hussein , Baraa A. Abdul Hameed , Wathiq Q. Shaker and Fathel A.Hasan (2014).Cytogenetic and hormonal study for sample of men infertile in Iraq. International Journal for Sciences and Technology, Vol. 9, 62-65.
● Abeer M. Badr, Mohammed M. F. Al-Halbosiy, Rashika El Ridi. (2015). Differential immune responses toexcretory─secretoryantigens of lung-stage larvae of Schistosoma mansoniin mice and rats. The Journal of Basic & Applied Zoology. 69, 26–33.
● Mohammed Adel Nouri, Mohammed M. F. Al-Halbosiy, Batol Imran Dheeb, Abdulkareem Jasim Hashim. (2015). Cytotoxicity and Genotoxicity of Gliotoxin on human lymphocytes in vitro. Journal of King Saud University – Science.
● Mohammad M. F. Al-Halbosiy, Rakad M. Kh. Al-Jumaily, Fadhel M . Lafta. (2015). The Exposure Effect of Water Pipe Smoke (WPS) on the Total Count leukocyte, Mitotic Index, Micronucleus Formation and Chromosome Aberration in Albino Male Mice. Journal of Biotechnology Research Center, Vol. 9, 89-93.
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● Rawaa N. Abdallah1, Raghad K. Allihaibi, Mohammad M.F. Al-Halbosiy and Ali A. Taha (2016). Preparation of Nanoliposomes by Size Exclusion Chromatography to Entrap Soluble Antigens from Leishmania donovani. Iraq Journal of Science (Vol. 57, , : 814-823).
● Niran A.H. Al-Ogaili, Mohammed M.F. Al-Halbosiy, Mahmoud Zayanal Mohammed, Zainab Al-Hawraa Mohammed, Afyaa Diyaa Rushk. (2017). IN VITRO INVESTIGATION OF ANTIBACTERIAL AND ANTILEISHMANIAL ACTIVITIES OF GUM-RESIN OF BOSWELLIA SERRATA USING (0.5%) DMSO SOLUTION AS AN ALTERNATIVE SOLVENT FOR EXTRACTION. World Journal of Pharmacy and Pharmaceutical Sciences, 6(9): 190-199.
● Hujran Abed Al-raheem Abed, Huda F Hasan and Mohammad MF Al-Halbosiy. (2017). Comparative effect between Mesenchymal Stem Cells (MSCs) and Clomiphine citrate on induced premature ovarian failure (POF) in mice. Journal of Entomology and Zoology Studies 2017; 5(5): 955-963. ● Huda F. Hasan, Esraa M. Krair, Mohammad M.F.Al-Halbosiy. (2017). Evaluating anti-anemic effect of Mesenchymal Stem Cells and Oxymetholone on aplastic anemia induced in mice.
● Rajwa Hasen Essa, Mohammad Mahmood F. Al-Halbosiy, Sundus Hameed Ahmed. (2017). Evaluation, Antioxidant, Antimitotic and Anticancer Activity of Iron Nanoparticles Prepared by Using Water Extract of Vitis vinifera L. Leaves. Journal of Advanced Laboratory Research in Biology, 8 (3) :67-73.
●Afnan R. A. Al-Tekreeti, Mohammad M. F. Al-Halbosiy, Batol I. Dheeb, Abdulkareem J. Hashim, Anaam F. H. Al-Zuhairi, Farooq
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I. Mohammad. (2018). Molecular identification of clinical Candida isolates by simple and randomly amplified polymorphic DNA-PCR. Arabian Journal for Science and Engineering 43: 163-170.
● Selma M.H. AL-Jawad, Ali A. Taha, Mohammad M.F. Al-Halbosiy, Lamyaa F.A. AL-Barram. (2018). Synthesis and characterization of small-sized gold nanoparticles coated by bovine serum albumin (BSA) for cancer photothermal therapy. Photodiagnosis and Photodynamic Therapy 21: 201–210.
● Huda F. Hasan, Esraa M. Krair, Mohammad M.F.Al-Halbosiy. Evaluating anti-anemic effect of Mesenchymal Stem Cells and Oxymetholone on aplastic anemia induced in mice. (2017).