Molecular distinction of two closely resembling Morinda species using rbcL and matK loci for quality management of Indian herbal medicines Rahul R. Nair, P. S. Udayan, S. Thilaga, M. Kavitha, R. M. Bharathanandhini, A. M. Nizzy, D. Ganesh Plant Genetic Resources Characterisation and Utilisation, 2013 Morinda reticulata Gamble and Morinda umbellata Linn. (Rubiaceae) are medicinally important climbers distributed as a mixed population in southern Western Ghats of India. A close morphological resemblance of these two species misleads the harvester in the identification of plant parts for preparation of herbal medicines. Though both species contain anthraquinone derivatives and share common medicinal properties for treating stomach disorders, each of these species has unique curative properties for treating selective diseases. Conventional methods are not reliable for identification of these species due to similarities in morphology. Thus, misidentification often leads to the deterioration of the quality of medicines. Thus, authentication utilizing conserved gene sequences in the chloroplast genome of these two Morinda spp. has been attempted for precise identification. Here we report the use of two barcoding genes (maturase kinase and ribulose 1,5-bisphosphate carboxylase large subunit) to distinguish M. reticulata and M. umbellata based on single nucleotide polymorphism. The present findings can be used for authenticating leaf samples of M. reticulata and M. umbellata.
Phytochemical characterization and antimicrobial activity of Curcuma xanthorrhiza Roxb. Helen PA Mary, Gomathy K Susheela, S Jayasree, AM Nizzy, B Rajagopal, S Jeeva Asian Pacific Journal of Tropical Biomedicine, 2012 Abstract Objective To study the antimicrobial activity and phytochemical characterization of essential oil isolated from the rhizome of Curcuma xanthorrhiza against pathogenic bacteria and fungi. Methods Fresh rhizomes of Curcuma xanthorrhiza were subjected to hydro distillation process to obtain essential oil and characterized by Gas Chromatography- Mass Spectroscopy (GC-MS). The essential oil was evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and six fungi by the disc diffusion method. Results GC – MS analysis of the essential oil extracted from the rhizome of Curcuma xanthorrhiza contained the derivatives of xanthorihizol, camphene and curcumene, monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene, hydrocarbons and other minor compounds. The antimicrobial activity of the oil showed significant inhibitory activity against the human pathogenic bacteria, no activity was observed against the fungi Aspergillus niger and Fusarium oxysporum. Conclusions The findings of the present study indicate that the rhizome extract of Curcuma xanthorrhiza possess secondary metabolites and potential to develop antimicrobial drugs.
Phytochemical characterization and antimicrobial activity of shampoo ginger (Zingiber zerumbet) from Tamil Nadu, India Asian Journal of Microbiology Biotechnology and Environmental Sciences, 2009
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