ALESSANDRA MARNIE MARTINS GOMES DE CASTRO

@unip.br

Stricto Sensu Graduate Program in Environmental and Experimental Pathology
Universidade de São Paulo

ALESSANDRA MARNIE MARTINS GOMES DE CASTRO


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https://researchid.co/alessandra.castro

RESEARCH, TEACHING, or OTHER INTERESTS

Animal Science and Zoology, Immunology and Microbiology, Virology, Veterinary
20

Scopus Publications

992

Scholar Citations

15

Scholar h-index

21

Scholar i10-index

Scopus Publications

  • Recent advances in veterinary diagnostic virology
    Anbu K. Karuppannan, Alessandra Marnie M. G. de Castro, Tanja Opriessnig
    Advanced Techniques in Diagnostic Microbiology, 2018
  • Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: First report
    Alessandra Marnie Martins Gomes de Castro, Josete Garcia Bersano, Vanessa Aparecida Feijó, Renato Akio Ogata, Julia Mitico Nara, et al.
    Semina Ciencias Agrarias, 2018
    Porcine parvovirus 4 (PPV4) has been reported in several countries and the high rate of concurrent PCV2 infection with PPV4 may trigger the “porcine circovirus disease” (PCVD). This has awakened the interest in how PPV4 virus behaves. The aim of this study was to show that, like other swine viruses, the elimination of PPV4 in semen is intermittent. The study was conducted in the Unidade de Pesquisa e Desenvolvimento de Itapeva da Agência Paulista de Tecnologia dos Agronegócios Regional (Apta Regional). Over a period of six months, four boars were monitored and the semen was collected every 10 days, totalizing 74 semen samples. Through PCR testing, PPV4 was detected in 32/74 (43.2%) semen samples. Three sequences of 284 nt, showed identity varying from 98.2% to 100%, confirming the detection of the virus in all boars. Semen volume and sperm motility did not indicate significant difference between PPV4 positive or negative semen samples (p > 0.05). There was no significant difference between the monthly distributions of positive and negative samples. This is the first study showing intermittent shedding of PPV4 in naturally infected boars. Absence of typical clinical signs and/or influence in semen quantity was also observed.
  • In vitro and in silico studies reveal capsid-mutant Porcine circovirus 2b with novel cytopathogenic and structural characteristics
    Taís Fukuta Cruz, Angelo José Magro, Alessandra M.M.G. de Castro, Francisco J. Pedraza-Ordoñez, Miriam Harumi Tsunemi, et al.
    Virus Research, 2018
  • Bovine vaccinia in dairy cattle and suspicion of vesicular disease on milkers in Brazil
    Thaís Garcia da Silva, Michele dos Santos Lima, Alessandra Marnie Martins Gomes de Castro, Maira de Souza Nunes Martins, Vivian Cardoso Castiglioni, et al.
    Ciencia Rural, 2018
    Bovine vaccinia (BV) is a vesicular disease induced by the Vaccinia virus (VACV) that affects milk production and is an occupational zoonosis. This research had the following objectives: (i) detection of VACV by qPCR in cattle with clinical suspicion of vesicular disease; (ii) symptoms characterization in animals and milkers with clinical suspicion of the disease and virus detection in humans; and (iii) identification of risk factors for infections of VACV in herds from several Brazilian states. A total of 471 bovine epithelial samples from dairy farms, in 15 Brazilian states, were evaluated between 2007 and 2012. The samples were tested by quantitative PCR (qPCR) using SYBR Green® reagents, validated with a lower limit of detection of 100TCID50/50µL (1.7x100 viral particles), and 45.1% of VACV positive samples were detected. Using official forms for epidemiological investigation (FORM-IN), the risk factors for VACV infections in cattle were determined to be farms with a lack of technological facilities (P= 0.029) and the presence of rodents (P= 0.001). There was an effect of seasonality in cattle with a higher occurrence of BV during the dry season. A total of 420 epidemiological questionnaires were applied at public health care centers, where 100% of the milkers had vesicular lesions on their hands (98.1%) and on their arms (6.9%). The most frequent clinical symptoms in humans were: local swelling (74.2%), headache (20.7%), fever (10.4%) and inguinal lymphadenopathy (74.2%). Only 19.98% of milkers aged between 39 and 58 years were seroreactive to VACV and were immunized with the human anti-smallpox vaccine. There was an increase in the frequency of BV in older individuals due to their natural decrease in specific immunity. It has been shown that the implementation of zootechnical management techniques and health planning are important for the prevention of BV in animals and humans.
  • Evaluation of the efficacy of a commercial inactivated genogroup 2b-based porcine epidemic diarrhea virus (PEDV) vaccine and experimental live genogroup 1b exposure against 2b challenge
    Tanja Opriessnig, Priscilla F. Gerber, Huigang Shen, Alessandra Marnie M. G. de Castro, Jianqiang Zhang, et al.
    Veterinary Research, 2017
  • An interferon inducing porcine reproductive and respiratory syndrome virus vaccine candidate elicits protection against challenge with the heterologous virulent type 2 strain VR-2385 in pigs
    Eve Fontanella, Zexu Ma, Yanjin Zhang, Alessandra M.M.G. de Castro, Huigang Shen, et al.
    Vaccine, 2017
  • Influence of the inclusion of spray-dried animal plasma in weanling pigs’ diet on porcine circovirus type 2 (PCV2) loads
    Renato Pacheco Monferdini, Alessandra Marnie Martins Gomes de Castro, Priscilla Freitas Gerber, Fernando Gomes de Castro Junior, Zélia Ines Portela Lobato, et al.
    Brazilian Journal of Veterinary Research and Animal Science, 2017
    O plasma sanguíneo em pó (PSP), produto natural de indústria frigorífica, tem mostrado efeitos benéficos sobre o crescimento e desempenho de leitões desmamados precocemente. Atualmente, embora o circovírus suíno 2 (PCV2) tenha grande importância para a suinocultura, não há informações sobre o impacto do uso de PSP e a resposta imune ao PCV2 em infecções naturais. Este trabalho avaliou diferentes níveis de inclusão de PSP em dietas de leitões e as cargas virais de PCV2 correspondentes. Quatro níveis de inclusão de PSP foram testados em dois períodos consecutivos: 0, 2, 4 ou 6% durante o período 1 (14 aos 28 dias de idade) e 1, 2 ou 3% de PSP durante o período 2 (29 a 42 dias de idade). No período 3 (42 aos 56 dias de idade), todos os leitões foram alimentados com dieta isenta de PSP. Amostras de soro foram coletadas semanalmente e testadas para anticorpos anti-PCV2 e carga de DNA de PCV2. As concentrações de 6% e 3% de PSP fornecidas nas rações durante o período 1 e 2, respectivamente, influenciaram na carga viral de PCV2 de suínos naturalmente infectados.
  • A double-antibody sandwich ELISA based on the porcine circovirus type 2 (PCV2) propagated in cell culture for antibody detection
    Taís F. Cruz, Tatiana M. Kanashiro, Alessandra M.M.G. de Castro, Cintia M. Baldin, Leonardo J. Richtzenhain, et al.
    Pesquisa Veterinaria Brasileira, 2016
    Few studies have described enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against porcine circovirus type 2 (PCV2) based on antigens produced in cell culture. Furthermore, few articles have described viral purification techniques for members of the family Circoviridae. This occurs because circoviruses are difficult to isolate, noncytopathogenic, and produce low viral titres in cell culture. Thus, for overcoming these difficulties in the cultivation of PCV2, this study aimed to develop a double-antibody sandwich ELISA based on the cell culture antigen PCV2b for the quantification of anti-PCV2 antibodies. A 20% and 50% discontinuous sucrose cushion was used for viral purification, which enabled the separation of cell culture proteins in the 20% sucrose cushion and a greater viral concentration in the 50% sucrose cushion. Following isopycnic centrifugation, PCV2 was concentrated in the band with density values from 1.330 to 1.395g/cm3. Viral purification was assessed using SDS-PAGE, indirect ELISA and electron microscopy. The standardised ELISA revealed a strong linear correlation (r= 0.826, p<0.001) when compared with a commercial ELISA kit. The assay exhibited low variability (inter-assay coefficient of variation of 4.24% and intra-assay of 1.80%) and excellent analytical specificity conferred by the capture antibody produced in rabbit. Thus, this ELISA is a rapid, specific and convenient method for the detection of antibodies against PCV2 in studies of experimental and natural infection, and in monitoring the response to vaccination on commercial farms.
  • Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice
    Alessandra M.M.G. de Castro, Taís F. Cruz, Katarina B. Yamada, Priscilla F. Gerber, Michelle P. Gabardo, et al.
    Research in Veterinary Science, 2015
  • Molecular diagnosis of Canine Leproid Granuloma out of paraffinembedded histological cuts using polymerase chain reaction (PCR) - A retrospective study (2002-2009)
    Simoni Maruyama, Paulo Eduardo Brandão, Alessandra Marnie Martins Gomes de Castro, Nilceo Schwery Michalany, Janet Fyfe, et al.
    Semina Ciencias Agrarias, 2015
    <p class="Pa7" style="margin-right: 28pt;"><span style="font-family: Verdana; color: black; font-size: 8pt;">O Granuloma lepróide canino (GLC) ou “lepra canina” consiste em micobacteriose, com quadro dermatopático que conta com aspectos ainda obscuros, principalmente quanto ao seu modo de transmissão e agente etiológico envolvido. Com a finalidade de se verificar a caracterização gênica da micobactéria em questão, procedeu-se estudo a partir de cortes histológicos, de animais com diagnóstico estabelecido da enfermidade. A amostragem foi composta de 13 cães, atendidos no Serviço de Dermatologia do HOVET/USP. Tal material foi posteriormente submetido às técnicas de reação em cadeia de polimerase (PCR), “nested” e em tempo real. O método da PCR “nested” confirmou a presença do agente em apenas 18,7% dos animais. Enquanto que, em 69,2% das amostras, o DNA da micobactéria envolvida foi detectado pela PCR em tempo real, demonstrando ser neste estudo, o método de maior sensibilidade. Pôde-se concluir ainda que a espécie de micobactéria causadora do Granuloma lepróide canino nos cães brasileiros possui homologia gênica de 100%, quando comparada às amostras provindas de animais de estudos estrangeiros. <strong></strong></span></p>
  • Revisiting the taxonomical classification of Porcine Circovirus type 2 (PCV2): Still a real challenge
    Giovanni Franzo, Martí Cortey, Alex Olvera, Dinko Novosel, Alessandra Marnie Martins Gomes De Castro, et al.
    Virology Journal, 2015
  • Torque teno sus virus 1 and 2 viral loads in faeces of porcine circovirus 2-positive pigs
    Alessandra M. M. G. de Castro, Cintia M. Baldin, Cintia M. Favero, Priscilla F. Gerber, Rafael I. Cipullo, et al.
    Acta Veterinaria Brno, 2015
  • Swine infectious agents in Tayassu pecari and pecari tajacu tissue samples from Brazil
    Alessandra Marnie Martins Gomes de Castro, Talita Brombila, Josete Garcia Bersano, Herbert Sousa Soares, Sheila Oliveira de Souza Silva, et al.
    Journal of Wildlife Diseases, 2014
  • A PCV2 vaccine based on genotype 2b is more effective than a 2a-based vaccine to protect against PCV2b or combined PCV2a/2b viremia in pigs with concurrent PCV2, PRRSV and PPV infection
    Tanja Opriessnig, Kevin O’Neill, Priscilla F. Gerber, Alessandra M.M.G. de Castro, Luis G. Gimenéz-Lirola, et al.
    Vaccine, 2013
  • Preliminary study of Porcine circovirus type 2 and Torque teno sus virus coinfection frequencies in Brazilian pig herds
    Canadian Journal of Veterinary Research, 2012
  • Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil.
    Alessandra MMG de Castro, Taís F Cruz, Vanessa R Salgado, Tatiana M Kanashiro, Karen L Ferrari, et al.
    Acta Veterinaria Scandinavica, 2012
  • The Cap gene of porcine circovirus type 2 (PCV2) evolves by positive selection in vitro
    Brazilian Journal of Veterinary Research and Animal Science, 2010
  • Molecular diversity of Brazilian strains of porcine circovirus type 2 (PCV-2)
    Alessandra Marnie Martins Gomes de Castro, Adriana Cortez, Marcos Bryan Heinemann, Paulo Eduardo Brandão, Leonardo José Richtzenhain
    Research in Veterinary Science, 2008
  • Genetic diversity of Brazilian strains of porcine circovirus type 2 (PCV-2) revealed by analysis of the cap gene (ORF-2)
    A. M. Martins Gomes de Castro, A. Cortez, M. B. Heinemann, P. E. Brandão, L. J. Richtzenhain
    Archives of Virology, 2007
  • Genetic characterization of Brazilian bovine viral diarrhea virus isolates by partial nucleotide sequencing of the 5′-UTR region
    Adriana Cortez, Marcos B Heinemann, Alessandra Marnie M.G. de Castro, Rodrigo M Soares, Ana Maria V. Pinto, et al.
    Pesquisa Veterinaria Brasileira, 2006

RECENT SCHOLAR PUBLICATIONS

  • LEISHMANIOSE VISCERAL CANINA EM SÃO PAULO: REVELANDO OS DESAFIOS DO DIAGNÓSTICO, TRATAMENTO E VIGILÂNCIA EM ÁREA URBANA NÃO ENDÊMICA
    CBF Martins, DF da Silva, TL Cortez, DRM Matias, VAF de Souza, ...
    Periódicos Brasil. Pesquisa Científica 5 (2), 233-246 , 2026
    2026
  • Cisticercose Suína: Análise de Falhas em Inspeção Sanitária de Abatedouros Legalizados e Abate Clandestino com Cartilha Educativa
    KA Alves, DF da Silva, AMMG de Castro
    Brazilian Journal of Biological Sciences 12 (27), e563-e563 , 2025
    2025
  • Canine circovirus: emergence, adaptation, and challenges for animal and public health
    DF Silva, M Ciola, VO Lopes, DRM Matias, TS Oliveira, AMMG Castro
    Frontiers in Veterinary Science 12, 1535650 , 2025
    2025
    Citations: 4
  • Erratum to'PCV2: The unexpected threat-How fish infection can transform industries and society: A pilot study'
    DF Silva, F Baldisseri, JCS Borges, LM de Barros, ZCP Caetano, ...
    South African Journal of Animal Science 55 (3), 139-139 , 2025
    2025
  • Vaccination strategies to curb environmental spread of Porcine Circovirus 2
    DF Silva, VH Silva, LM Barros, TS Oliveira, CIS Macedo, RG Mesquita, ...
    South African Journal of Animal Science 54 (4) , 2024
    2024
  • Porcine circovirus 3: a new challenge to explore (vol 10, 1266499, 2024)
    RR da Silva, DF da Silva, VH da Silva, AMMG de Castro
    FRONTIERS IN VETERINARY SCIENCE 11 , 2024
    2024
  • Corrigendum: Porcine circovirus 3: a new challenge to explore
    RR Silva, DF Silva, VH Silva, AMMG Castro
    Frontiers in Veterinary Science 11, 1448165 , 2024
    2024
  • Porcine circovirus 3: a new challenge to explore
    RR Silva, DF Silva, VH Silva, AMMG Castro
    Frontiers in Veterinary Science 10, 1266499 , 2024
    2024
    Citations: 14
  • Porcine circovirus 3: a new
    RR da Silva, DF da Silva, VH da Silva, AMMG de Castro
    2024
  • PCV2: The unexpected threat-How fish infection can transform industries and society: A pilot study
    DF Silva, F Baldisseri, VH Silva, JCS Borges, LM de Barros, ZCP Caetano, ...
    South African Journal of Animal Science 54 (1), 93-99 , 2024
    2024
  • Porcine circovirus 2 : current status and its challenges.
    RR da Silva, AMMG de Castro
    2022
  • Porcine circovirus 2: Status atual e seus desafios
    RR da Silva, AMMG de Castro
    Pubvet 16 (04) , 2022
    2022
    Citations: 3
  • High genetic diversity of hepatitis E virus in swine in São Paulo State, Brazil
    A Cortez, CS Metorima, SAT Miyagi, AO Sousa, AV Peyser, AMMG Castro, ...
    Arquivo Brasileiro de Medicina Veterinária e Zootecnia 73, 1237-1242 , 2021
    2021
    Citations: 2
  • Diversidade genética do vírus da hepatite E em suínos no estado de São Paulo, Brasil
    A Cortez, CS Metorima, SAT Miyagi, AO Sousa, AV Peyser, A Castro, ...
    Arquivo Brasileiro de Medicina Veterinária e Zootecnia 73, 1237-1242 , 2021
    2021
  • Circovirus porcinos: Estado actual, vacíos en el conocimiento y desafíos
    T Opriessnig, AK Karuppannan, AMMG de Castro, CT Xiao
    Suis, 22-25 , 2021
    2021
  • Emergence of zoonoses in disasters: a case report of leptospirosis in Brumadinho, Minas Gerais.
    AR Silvestrini, AC Paes, AMMG de Castro
    2020
  • Leptospirosis: bibliographic analysis, serological survey and canine vaccination.
    AR Silvestrini, A Castro, H Langoni
    2020
  • Leptospirose: análise bibliográfica, inquérito sorológico e vacinação canina
    AR Silvestrini, A Castro, H Langoni
    Ars Veterinaria 36 (4), 286-300 , 2020
    2020
    Citations: 2
  • Porcine circoviruses: current status, knowledge gaps and challenges
    T Opriessnig, AK Karuppannan, AMMG Castro, CT Xiao
    Virus research 286, 198044 , 2020
    2020
    Citations: 247
  • Emergência de zoonoses em desastres: Relato de caso de leptospirose em Brumadinho, Minas Gerais
    AR Silvestrini, AC Paes, AMMG de Castro
    Pubvet 15, 143 , 2020
    2020
    Citations: 2

MOST CITED SCHOLAR PUBLICATIONS

  • Porcine circoviruses: current status, knowledge gaps and challenges
    T Opriessnig, AK Karuppannan, AMMG Castro, CT Xiao
    Virus research 286, 198044 , 2020
    2020
    Citations: 247
  • A PCV2 vaccine based on genotype 2b is more effective than a 2a-based vaccine to protect against PCV2b or combined PCV2a/2b viremia in pigs with concurrent PCV2, PRRSV and PPV …
    T Opriessnig, K O’Neill, PF Gerber, AMMG de Castro, LG Gimenéz-Lirola, ...
    Vaccine 31 (3), 487-494 , 2013
    2013
    Citations: 115
  • Revisiting the taxonomical classification of Porcine Circovirus type 2 (PCV2): still a real challenge
    G Franzo, M Cortey, A Olvera, D Novosel, AMMG De Castro, P Biagini, ...
    Virology journal 12 (1), 131 , 2015
    2015
    Citations: 102
  • Evaluation of the efficacy of a commercial inactivated genogroup 2b-based porcine epidemic diarrhea virus (PEDV) vaccine and experimental live genogroup 1b exposure against 2b …
    T Opriessnig, PF Gerber, H Shen, AMMG De Castro, J Zhang, Q Chen, ...
    Veterinary research 48 (1), 69 , 2017
    2017
    Citations: 54
  • Genetic diversity of Brazilian strains of porcine circovirus type 2 (PCV-2) revealed by analysis of the cap gene (ORF-2)
    AM Martins Gomes de Castro, A Cortez, MB Heinemann, PE Brandão, ...
    Archives of Virology 152 (8), 1435-1445 , 2007
    2007
    Citations: 49
  • Swine infectious agents in Tayassu pecari and Pecari tajacu tissue samples from Brazil
    AMMG de Castro, T Brombila, JG Bersano, HS Soares, ...
    The Journal of Wildlife Diseases 50 (2), 205-209 , 2014
    2014
    Citations: 36
  • Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil
    AMMG de Castro, TF Cruz, VR Salgado, TM Kanashiro, KL Ferrari, ...
    Acta Veterinaria Scandinavica 54 (1), 29 , 2012
    2012
    Citations: 27
  • Detecção de ácidos nucléicos de Brucella spp., Leptospira spp., herpesvirus bovino e vírus da diarréia viral bovina, em fetos bovinos abortados e em animais mortos no perinatal
    A Cortez, AMG Castro, MB Heinemann, RM Soares, RC Leite, E Scarcelli, ...
    Arquivo Brasileiro de Medicina Veterinária e Zootecnia 58 (6), 1226-1228 , 2006
    2006
    Citations: 24
  • Malignant catarrhal fever in Brazilian cattle presenting with neurological syndrome
    M de SN Martins, AMMG de Castro, MS Lima, V da SC Pinto, TG da Silva, ...
    brazilian journal of microbiology 48 (2), 366-372 , 2017
    2017
    Citations: 21
  • Mast cell and eosinophils in the wall of the gut and eosinophils in the blood stream during Toxocara vitulorum infection of the water buffalo calves (Bubalus bubalis)
    MF Neves, WA Starke-Buzetti, AMMG Castro
    Veterinary Parasitology 113 (1), 59-72 , 2003
    2003
    Citations: 18
  • A Porcine circovirus type 2b (PCV2b)-based experimental vaccine is effective in the PCV2b-Mycoplasma hyopneumoniae coinfection pig model
    T Opriessnig, AMMG Castro, AK Karuppanan, PC Gauger, PG Halbur, ...
    Vaccine 37 (44), 6688-6695 , 2019
    2019
    Citations: 16
  • An interferon inducing porcine reproductive and respiratory syndrome virus vaccine candidate elicits protection against challenge with the heterologous virulent type 2 strain …
    E Fontanella, Z Ma, Y Zhang, AMMG de Castro, H Shen, PG Halbur, ...
    Vaccine 35 (1), 125-131 , 2017
    2017
    Citations: 16
  • Detection of genetic characterization of porcine circovirus 2 (PCV2) in Brazilian wildlife boars
    A Castro, FG Castro Jr, FEL Budiño, CM Baldin, SOS Silva, PE Brandão, ...
    Brazilian Journal of Microbiology 43 (3), 1022-1025 , 2012
    2012
    Citations: 16
  • Fibra na alimentação de suínos
    FG de Castro Júnior, JC de Moura Camargo, AMMG de Castro, ...
    Boletim de Indústria Animal 62 (3), 265-280 , 2005
    2005
    Citations: 16
  • Leptospirose no contexto da Saúde Única e diretrizes de vacinação
    AR Silvestrini, MB Heinemann, AMMG Castro
    Pubvet. Publicações em Medicina Veterinária e Zootecnia 14 (2), 1-8 , 2020
    2020
    Citations: 15
  • First detection of the equine herpesvirus 1 neuropathogenic variant in Brazil
    E Mori, AS Borges, DJZ Delfiol, JP Oliveira Filho, RC Goncalves, ...
    Revue Scientifique et Technique-OIE 30 (3), 949 , 2011
    2011
    Citations: 15
  • Molecular diversity of Brazilian strains of porcine circovirus type 2 (PCV-2)
    AMMG de Castro, A Cortez, MB Heinemann, PE Brandão, ...
    Research in veterinary science 85 (1), 197-200 , 2008
    2008
    Citations: 15
  • Circovírus suíno tipo 2 (PCV-2)
    A Castro, A Cortez, MB Heinemann, P Brandão, LJ Richtzenhain
    Arquivos do Instituto Biológico 74 (3), 281-291 , 2007
    2007
    Citations: 15
  • Porcine circovirus 3: a new challenge to explore
    RR Silva, DF Silva, VH Silva, AMMG Castro
    Frontiers in Veterinary Science 10, 1266499 , 2024
    2024
    Citations: 14
  • Detection and differentiation of porcine circoviruses in Brazilian pigs.
    AMMG Castro, A Cortez, VLA Ruiz, H Leomil, AM Moreno, DS Doto, ...
    2004
    Citations: 14