EFFECT OF DIFFERENT LEVELS OF SALT AND DROUGHT STRESSES ON GENE EXPRESSION OF TWO TOLERANCE-DIFFERENT TOMATO CULTIVARS IN VITRO L. K. J. Al-Amery, Ali H Annon Iraqi Journal of Agricultural Sciences, 2024 A lab experiment was conducted at the Plant Tissue Culture Lab / College of Agricultural Engineering Sciences / University of Baghdad. This experiment was aimed to investigate gene expression index in tomato (Solanum lycopersicum L.) after preparation of salt and simulated drought stresses. Two tomato cultivars were selected which claimed to exhibit different levels of tolerance toward abiotic stresses designated as salt-tolerant Yassamine (Y) and salt-sensitive GS12 (G) to assess the test. Seven day-old seedlings from both cultivars were grown in MS media supplemented with four concentrations of NaCl at 0, 50, 100 and 150 mM and four concentrations of PEG at 0, 10, 20, and 30% for 48 hours. The results were showed that Y cultivar exhibited more proline secretion and chlorophyll content when compared with G. In addition, Y cultivar showed less ion leakage and less affected by elevated abiotic stresses in term of seedling weight variation when compared to G counterparts. The SDS-PAGE gel analysis showed that Y cultivar showed more band intensity when compared with G suggested more corresponding gene expression of tolerant protein against abiotic stresses.
PERFORMANCE AND MOLECULAR ANALYSIS OF POTATO LINES DEVELOPED FROM GAMMA RAYS AND EMS APPLICATIONS Annon & Abdulrasool Iraqi Journal of Agricultural Sciences, 2020 An experiment was conducted in a greenhouse - research station B - College of Agricultural Engineering Sciences, University of Baghdad, during the fall season of 2018 with the aim of propagating and initially studying the field performance of 18 and 20 potential potato lines derived from Rivera and Arizona cv. after in vitro exposure of nodal segments to different dosages of gamma rays (0, 10, 20, and 30 Gray) and EMS (0, 10, 20, and 30 mM). Each control cultivar and their derived lines were independently cultured in plastic bags according to the RCBD, with three replications. The results showed that the highest plant height and number of leaves were obtained from Arizona derived lines which gave 60.11 cm and 25.30 leaves.plant-1 in lines 207 and 222, respectively when compared with their control that gave 38.11 cm and 13.67 leaves.plant-1, respectively. Minitubers diameter, weight, and plant yield were in its highest values in Arizona derived lines 551, 551, and 459 which gave 35.73 mm.minituber-1, 33.13 g. minituber-1, and 133.8 g.plant-1, respectively compared to their control that gave 25.35 mm.minituber, 16.8 g.minituber, and 78.57 g.plant-1, respectively. The resulted lines were analyzed at the molecular level utilizing the inter simple sequence repeats (ISSR) markers and revealed that lines 69, 10, 68, 102, and 7 were the much distanced from its derived Rivera cultivar and gave 40.7%, 37.1%, 36.8%, 33.3%, and 30.0%, respectively while lines 551, 261, 474, and 254 were the much genetically distanced from their derived cultivar Arizona with genetic distances of 24.1%, 22.6%, 18.8%, and 17.6%, respectively.
Effect of gamma radiation and ethyl methanesulfonate (EMS) on potato salt stress tolerance In Vitro Annon & Abdulrasool Iraqi Journal of Agricultural Sciences, 2020 An experiment was conducted in the Date Palm Research Units labs / College of Agricultural Engineering Sciences / University of Baghdad to assess the tolerance toward salinity stress in potato after two mutagens treatments in vitro. Potato cv. Arizona and Rivera nodal segments were irradiated with four dosages of gamma rays at 0, 10, 20, and 30 Gray and immersed in (EMS) with four concentrations included 0, 10, 20, and 30 mM. The survival rates after mutagenesis treatments were calculated and 449 lines were obtained. The lines were tested for salinity tolerance by growing in MS medium supplemented with four concentrations of NaCl at 0, 100, 150, and 200 mM and data were analyzed according to the CRD with 10 replicates and means were compared according to LSD at 5%. Out of 449 lines obtained, only 38 lines were showed to be tolerant to salinity stress depending on some vegetative characteristics. At the highest NaCl concentration of 200 mM, Almost all derived lines were higher when compared with their controls especially the lines that derived from Arizona in which line 262 gave the most significant plantlets height of 35 mm and line 180 gave the most significant number of nodes of 3.66 nodes.plant-1 and number of shoots of 2 shoots.plant-1 when compared with their control that gave 11.33 mm, 2 nodes.plant-1, and 1.5 shoots.plant-1, respectively. The resulted lines were analyzed at the molecular level utilizing the inter simple sequence repeats (ISSR) markers and revealed that line 69 was the much distanced from its derived Rivera cultivar while lines 551, 261, 170, 262, 459, 463 were the much genetically distanced from their derived cultivar Arizona.
Effect of three honeycomb interplant distances on growth and flowering of two cultivars of bean AMA Agricultural Mechanization in Asia Africa and Latin America, 2016
Effect of three honeycomb interplant distances on yield and it components of two cultivars of bean AMA Agricultural Mechanization in Asia Africa and Latin America, 2015
