In Vivo Assessment of Silica-coated Titanium Implants for Improved Osseointegration: a Systematic Review Giovanna do Espirito Santo, Amanda Sardeli Alqualo, Gustavo Oliva Amaral, Amanda de Souza, Homero Garcia-Motta, Julieta Leticia Merlo, Beatriz Louise Mendes Viegas, João Paulo dos Santos Prado, Karolyne dos Santos Jorge Sousa, Rafael Anaya da Silva Zaratin, Renata Neves Granito, Daniel Araki Ribeiro, Silvia Marcela Ceré, Ana Claudia Rennó Regenerative Engineering and Translational Medicine, 2026
Development and Comparison of Two 3D-Printed Scaffolds of Biosilica from Marine Sponges for Bone Tissue Engineering Giovanna do Espirito Santo, Amanda de Souza, Gustavo Oliva Amaral, Amanda Sardeli Alqualo, Karolyne dos Santos Jorge Sousa, Beatriz Louise Mendes Viegas, João Paulo dos Santos Prado, Francisco Vieira dos Santos, Daniel Souza Correa, Renata Neves Granito, Ana Claudia Muniz Rennó ACS Omega, 2025 This study compared the physicochemical characteristics and biological effects of two 3D-printed biosilica (BS) scaffolds (grid and gyroid). The methods included scanning electron microscopy (SEM), porosity, mass loss, pH assessment, Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray spectroscopy (EDS). The mechanical evaluation involved a compression test, and the in vitro tests used cell adhesion assays with osteoblastic and fibroblastic cell lines. SEM showed BS spicules in both models at day 0 with signs of degradation throughout the experimental immersion periods, forming a homogeneous network with interaction with alginate. Porosity measurements showed an average of 85.9% ± 0.9 for the grid model and 83.6% ± 0.7 for the gyroid model. The gyroid model demonstrated higher values in the compression test, a decrease in pH on day 1, and no difference for both models on days 3, 7, and 14. Mass loss was greatest in the gyroid model on day 21. FTIR tests showed characteristic peaks for ALG and BS. EDS detected silica (Si), chlorine (Cl), and calcium (Ca). In the cell adhesion assay, both models supported the adhesion and proliferation of L929 (fibroblast) and MC3T3-E1 (osteoblastic) cells, with the gyroid model showing better elongation and cell morphology. Overall, the gyroid model showed better physicochemical properties, higher mechanical strength, and improved biological performance compared to the grid model, making it a promising option for tissue engineering.
Exploring Antibacterial Properties of Marine Sponge-Derived Natural Compounds: A Systematic Review Cintia Cristina Santi Martignago, Camila de Souza Barbosa, Homero Garcia Motta, Beatriz Soares-Silva, Erica Paloma Maso Lopes Peres, Lais Caroline Souza e Silva, Mirian Bonifácio, Karolyne dos Santos Jorge Sousa, Amanda Sardeli Alqualo, Júlia Parisi, Olivier Jordan, Ana Claudia Muniz Renno, Anna Caroline Campos Aguiar, Viorica Patrulea Marine Drugs, 2025 The rise in multidrug-resistant (MDR) bacteria has prompted extensive research into antibacterial compounds, as these resistant strains compromise current treatments. This resistance leads to prolonged hospitalization, increased mortality rates, and higher healthcare costs. To address this challenge, the pharmaceutical industry is increasingly exploring natural products, particularly those of marine origin, as promising candidates for antimicrobial drugs. Marine sponges, in particular, are of interest because of their production of secondary metabolites (SM), which serve as chemical defenses against predators and pathogens. These metabolites exhibit a wide range of therapeutic properties, including antibacterial activity. This systematic review examines recent advancements in identifying new sponge-derived compounds with antimicrobial activity, specifically targeting Pseudomonas aeruginosa, a prevalent Gram-negative pathogen with the highest incidence rates in clinical settings. The selection criteria focused on antimicrobial compounds with reported Minimum Inhibitory Concentration (MIC) values. The identified SM include alkaloids, sesterterpenoids, nitrogenous diterpene, and bromotyrosine-derived derivatives. The structural features of the active compounds selected in this review may provide a foundational framework for developing new, highly bioactive antimicrobial agents.
RECENT SCHOLAR PUBLICATIONS
In vivo assessment of silica-coated titanium implants for improved osseointegration: a systematic review G do Espirito Santo, AS Alqualo, GO Amaral, A de Souza, H Garcia-Motta, ... Regenerative Engineering and Translational Medicine 12 (1), 135-151 , 2026 2026 Citations: 3
Development and Comparison of Two 3D-Printed Scaffolds of Biosilica from Marine Sponges for Bone Tissue Engineering G do Espirito Santo, A de Souza, GO Amaral, AS Alqualo, KSJ Sousa, ... ACS omega 10 (20), 20257-20267 , 2025 2025 Citations: 1
Exploring antibacterial properties of marine sponge-derived natural compounds: a systematic review CC Santi Martignago, C de Souza Barbosa, H Garcia Motta, ... Marine Drugs 23 (1), 43 , 2025 2025 Citations: 9
In Vivo Assessment of Silica-coated Titanium Implants for Improved Osseointegration: a Systematic Review GE Santo, AS Alqualo, GO Amaral, A de Souza, H Garcia-Motta, JL Merlo, ... REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE, 17 , 2025 2025
Development and comparison of two 3D printed scaffolds of biosilica from marine sponges for bone tissue engineering S GdE, A de Souza, GO Amaral, AS Alqualo, S KdSJ, BLM Viegas, ... 2024
Cell interactions and osteogenic differentiation on marine sponge-derived scaffolds: a systematic review J de Araújo Silva, AFSB da Silva, AS Alqualo, B Viegas, ACM Rennó, ... Biofunctional Materials 1 (1), 20-21 , 2023 2023 Citations: 3
MOST CITED SCHOLAR PUBLICATIONS
Exploring antibacterial properties of marine sponge-derived natural compounds: a systematic review CC Santi Martignago, C de Souza Barbosa, H Garcia Motta, ... Marine Drugs 23 (1), 43 , 2025 2025 Citations: 9
In vivo assessment of silica-coated titanium implants for improved osseointegration: a systematic review G do Espirito Santo, AS Alqualo, GO Amaral, A de Souza, H Garcia-Motta, ... Regenerative Engineering and Translational Medicine 12 (1), 135-151 , 2026 2026 Citations: 3
Cell interactions and osteogenic differentiation on marine sponge-derived scaffolds: a systematic review J de Araújo Silva, AFSB da Silva, AS Alqualo, B Viegas, ACM Rennó, ... Biofunctional Materials 1 (1), 20-21 , 2023 2023 Citations: 3
Development and Comparison of Two 3D-Printed Scaffolds of Biosilica from Marine Sponges for Bone Tissue Engineering G do Espirito Santo, A de Souza, GO Amaral, AS Alqualo, KSJ Sousa, ... ACS omega 10 (20), 20257-20267 , 2025 2025 Citations: 1
In Vivo Assessment of Silica-coated Titanium Implants for Improved Osseointegration: a Systematic Review GE Santo, AS Alqualo, GO Amaral, A de Souza, H Garcia-Motta, JL Merlo, ... REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE, 17 , 2025 2025
Development and comparison of two 3D printed scaffolds of biosilica from marine sponges for bone tissue engineering S GdE, A de Souza, GO Amaral, AS Alqualo, S KdSJ, BLM Viegas, ... 2024
