Amanda Rabello Crisma

EDUCATION

Bachelor in Pharmacy and Biochemistry at University of São Paulo (2005)
PhD in Sciences at Department of Clinical Analyses, University of São Paulo (2010)
Postdoc fellow at Department of Physiology and Biophysics, University of São Paulo (2011-2017)

RESEARCH, TEACHING, or OTHER INTERESTS

Immunology, Physiology, Hematology, Nutrition and Dietetics

Scopus Publications

Evidence for a Pro-Inflammatory State of Macrophages from Non-Obese Type-2 Diabetic Goto-Kakizaki Rats
Amanda Santos de Almeida Silveira, Amara Cassandra dos Anjos Alves, Gabriela Mandú Gimenes, Patrícia da Silva Quessada, Tiago Bertola Lobato, Beatriz Belmiro Dias, Ana Carolina Gomes Pereira, Patrícia Nancy Iser-Bem, Joice Naiara Bertaglia Pereira, Elaine Hatanaka,et al.
MDPI AG
Obesity causes insulin resistance (IR) through systemic low-grade inflammation and can lead to type 2 diabetes mellitus (T2DM). However, the mechanisms that cause IR and T2DM in non-obese individuals are unclear. The Goto-Kakizaki (GK) rat develops IR spontaneously and is a model of non-obese T2DM. These rats exhibit hyperglycemia beginning at weaning and exhibit lower body mass than control Wistar rats. Herein, we tested the hypothesis that macrophages of GK rats are permanently in a pro-inflammatory state, which may be associated with a systemic inflammation condition that mimics the pathogenesis of obesity-induced T2DM. Using eighteen-week-old GK and control Wistar rats, we investigated the proportions of M1 (pro-inflammatory) and M2 (anti-inflammatory) macrophages isolated from the peritoneal cavity. Additionally, the production of inflammatory cytokines and reactive oxygen species (ROS) in cultured macrophages under basal and stimulated conditions was assessed. It was found that phorbol myristate acetate (PMA) stimulation increased GK rat macrophage ROS production 90-fold compared to basal levels. This response was also three times more pronounced than in control cells (36-fold). The production of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α), tended to be upregulated in cultured macrophages from GK rats under basal conditions. Macrophages from GK rats produced 1.6 times more granulocyte-macrophage colony-stimulating factor (GM-CSF), 1.5 times more monocyte chemoattractant protein-1 (MCP-1) and 3.3 times more TNF-α than control cells when stimulated with lipopolysaccharide (LPS) (p = 0.0033; p = 0.049; p = 0.002, respectively). Moreover, compared to control cells, GK rats had 60% more M1 (p = 0.0008) and 23% less M2 (p = 0.038) macrophages. This study is the first to report macrophage inflammatory reprogramming towards a pro-inflammatory state in GK rats.


Maternal low-protein diet reduces skeletal muscle protein synthesis and mass via Akt-mTOR pathway in adult rats
Diogo Antonio Alves de Vasconcelos, Renato Tadeu Nachbar, Carlos Hermano Pinheiro, Cátia Lira do Amaral, Amanda Rabello Crisma, Kaio Fernando Vitzel, Phablo Abreu, Maria Isabel Alonso-Vale, Andressa Bolsoni Lopes, Adriano Bento-Santos,et al.
Frontiers Media SA
Several studies have demonstrated that a maternal low-protein diet induces long-term metabolic disorders, but the involved mechanisms are unclear. This study investigated the molecular effects of a low-protein diet during pregnancy and lactation on glucose and protein metabolism in soleus muscle isolated from adult male rats. Female rats were fed either a normal protein diet or low-protein diet during gestation and lactation. After weaning, all pups were fed a normal protein diet until the 210th day postpartum. In the 7th month of life, mass, contractile function, protein and glucose metabolism, and the Akt-mTOR pathway were measured in the soleus muscles of male pups. Dry weight and contractile function of soleus muscle in the low-protein diet group rats were found to be lower compared to the control group. Lipid synthesis was evaluated by measuring palmitate incorporation in white adipose tissue. Palmitate incorporation was higher in the white adipose tissue of the low-protein diet group. When incubated soleus muscles were stimulated with insulin, protein synthesis, total amino acid incorporation and free amino acid content, glucose incorporation and uptake, and glycogen synthesis were found to be reduced in low-protein diet group rats. Fasting glycemia was higher in the low-protein diet group. These metabolic changes were associated with a decrease in Akt and GSK-3β signaling responses to insulin and a reduction in RPS6 in the absence of the hormone. There was also notably lower expression of Akt in the isolated soleus muscle of low-protein diet group rats. This study is the first to demonstrate how maternal diet restriction can reduce skeletal muscle protein and mass by downregulating the Akt-mTOR pathway in adulthood.


Propolis authentication of stingless bees by mid-infrared spectroscopy and chemometric analysis
Monica Surek, Alexandre de Fátima Cobre, Mariana Millan Fachi, Thalita Gilda Santos, Roberto Pontarolo, Amanda Rabello Crisma, Karina Bettega Felipe, and Wesley Maurício de Souza
Elsevier BV



The toxicity of Aspidosperma subincanum to MCF7 cells is related to modulation of oxidative status and proinflammatory pathways
Andressa F. Santos, Nádia S.R. Santos Mota, Elberth M. Schiefer, Regiane S. da Cunha, Allan M. Junkert, Andréa E.M. Stinghen, Roberto Pontarolo, Amanda R. Crisma, Almeriane M. Weffort-Santos, Rozangela C. Pedrosa,et al.
Elsevier BV



Changes in PGC-1α-Dependent Mitochondrial Biogenesis Are Associated with Inflexible Hepatic Energy Metabolism in the Offspring Born to Dexamethasone-Treated Mothers
Carolina Vieira Campos, Caio Jordão Teixeira, Tanyara Baliani Payolla, Amanda Rabello Crisma, Gilson Masahiro Murata, Andressa Godoy Amaral, Lucas Carminatti Pantaleão, Frhancielly Shirley Sodré, Mariana Mayumi Onari, Lorena de Souza Almeida,et al.
MDPI AG
In the present study we investigated the participation of hepatic peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) in the metabolic programming of newborn rats exposed in utero to dexamethasone (DEX). On the 21st day of life, fasted offspring born to DEX-treated mothers displayed increased conversion of pyruvate into glucose with simultaneous upregulation of PEPCK (phosphoenolpyruvate carboxykinase) and G6Pase (glucose-6-phosphatase). Increased oxidative phosphorylation, higher ATP/ADP ratio and mitochondrial biogenesis and lower pyruvate levels were also found in the progeny of DEX-treated mothers. On the other hand, the 21-day-old progeny of DEX-treated mothers had increased hepatic triglycerides (TAG) and lower CPT-1 activity when subjected to short-term fasting. At the mechanistic level, rats exposed in utero to DEX exhibited increased hepatic PGC-1α protein content with lower miR-29a-c expression. Increased PGC-1α content was concurrent with increased association to HNF-4α and NRF1 and reduced PPARα expression. The data presented herein reveal that changes in the transcription machinery in neonatal liver of rats born to DEX-treated mothers leads to an inflexible metabolic response to fasting. Such programming is hallmarked by increased oxidative phosphorylation of pyruvate with impaired FFA oxidation and hepatic TAG accumulation.


Correction to: A probiotic has differential effects on allergic airway inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome (Microbiome, (2021), 9, 1, (134), 10.1186/s40168-021-01081-2)
Mateus B. Casaro, Andrew M. Thomas, Eduardo Mendes, Claudio Fukumori, Willian R. Ribeiro, Fernando A. Oliveira, Amanda R. Crisma, Gilson M. Murata, Bruna Bizzarro, Anderson Sá-Nunes,et al.
Microbiome Springer Science and Business Media LLC
© The Author(s). 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. The original article can be found online at https://doi.org/10.1186/s40168021-01081-2.


A probiotic has differential effects on allergic airway inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome
Mateus B. Casaro, Andrew M. Thomas, Eduardo Mendes, Claudio Fukumori, Willian R. Ribeiro, Fernando A. Oliveira, Amanda R. Crisma, Gilson M. Murata, Bruna Bizzarro, Anderson Sá-Nunes,et al.
Microbiome Springer Science and Business Media LLC
AbstractThe phenotypes of allergic airway diseases are influenced by the interplay between host genetics and the gut microbiota, which may be modulated by probiotics. We investigated the probiotic effects on allergic inflammation in A/J and C57BL/6 mice. C57BL/6 mice had increased gut microbiota diversity compared to A/J mice at baseline. Acetate producer probiotics differentially modulated and altered the genus abundance of specific bacteria, such as Akkermansia and Allistipes, in mouse strains. We induced airway inflammation followed by probiotic treatment and found that only A/J mice exhibited decreased inflammation, and the beneficial effects of probiotics in A/J mice were partially due to acetate production. To understand the relevance of microbial composition colonization in the development of allergic diseases, we implanted female C57BL/6 mice with A/J embryos to naturally modulate the microbial composition of A/J mice, which increased gut microbiota diversity and reduced eosinophilic inflammation in A/J. These data demonstrate the central importance of microbiota to allergic phenotype severity.


Immunomodulatory and cytotoxic activities of euphol
Thais Latansio de Oliveira, Lorena Bavia, Pâmela Dias Fontana, Luiza Stolz Cruz, Katia Sabrina Paludo, Amanda Rabello Crisma, Iara Jose Messias-Reason, and Flávio Luís Beltrame
Elsevier BV



Chemical composition, cytotoxicity, and antibacterial activity of propolis from Africanized honeybees and three different Meliponini species
Monica Surek, Mariana M. Fachi, Alexandre de Fátima Cobre, Favízia F. de Oliveira, Roberto Pontarolo, Amanda R. Crisma, Wesley M. de Souza, and Karina B. Felipe
Elsevier BV



Effects of Euphorbia umbellata extracts on complement activation and chemotaxis of neutrophils
Thais Latansio de Oliveira, Pâmela Dias Fontana, Lorena Bavia, Luiza Stolz Cruz, Amanda Rabello Crisma, Guilherme Lanzi Sassaki, Leociley Rocha Alencar Menezes, Mei Wang, Flávio Luís Beltrame, and Iara Jose Messias-Reason
Elsevier BV



Macrophage inflammatory state in Type 1 diabetes: Triggered by NLRP3/iNOS pathway and attenuated by docosahexaenoic acid
Mariana Rodrigues Davanso, Amanda Rabello Crisma, Tárcio Teodoro Braga, Laureane Nunes Masi, Cátia Lira do Amaral, Vinícius Nunes Cordeiro Leal, Dhêmerson Souza de Lima, Thiago Andrade Patente, José Alexandre Barbuto, Maria L. Corrêa-Giannella,et al.
Clinical Science Portland Press Ltd.
Abstract Type 1 diabetes mellitus (T1D) is a chronic autoimmune disease characterized by insulin-producing pancreatic β-cell destruction and hyperglycemia. While monocytes and NOD-like receptor family-pyrin domain containing 3 (NLRP3) are associated with T1D onset and development, the specific receptors and factors involved in NLRP3 inflammasome activation remain unknown. Herein, we evaluated the inflammatory state of resident peritoneal macrophages (PMs) from genetically modified non-obese diabetic (NOD), NLRP3-KO, wild-type (WT) mice and in peripheral blood mononuclear cells (PBMCs) from human T1D patients. We also assessed the effect of docosahexaenoic acid (DHA) on the inflammatory status. Macrophages from STZ-induced T1D mice exhibited increased inflammatory cytokine/chemokine levels, nitric oxide (NO) secretion, NLRP3 and iNOS protein levels, and augmented glycolytic activity compared to control animals. In PMs from NOD and STZ-induced T1D mice, DHA reduced NO production and attenuated the inflammatory state. Furthermore, iNOS and IL-1β protein expression levels and NO production were lower in the PMs from diabetic NLRP3-KO mice than from WT mice. We also observed increased IL-1β secretion in PBMCs from T1D patients and immortalized murine macrophages treated with advanced glycation end products and palmitic acid. The present study demonstrated that the resident PMs are in a proinflammatory state characterized by increased NLRP3/iNOS pathway-mediated NO production, up-regulated proinflammatory cytokine/chemokine receptor expression and altered glycolytic activity. Notably, ex vivo treatment with DHA reverted the diabetes-induced changes and attenuated the macrophage inflammatory state. It is plausible that DHA supplementation could be employed as adjuvant therapy for treating individuals with T1D.


Schiefer counter: An alternative method for clonogenic assay evaluation
A.F. Santos, E.M. Schiefer, M.C. Atherino, J.C. Atherino, L.H. Negri, A.M. Weffort-Santos, A.R. Crisma, W.M. de Souza, and K.B. Felipe
Elsevier BV



Tributyrin Attenuates Metabolic and Inflammatory Changes Associated with Obesity through a GPR109A-Dependent Mechanism
Fabio Takeo Sato, Yu Anne Yap, Amanda Rabello Crisma, Mariana Portovedo, Gilson Masahiro Murata, Sandro Massao Hirabara, Willian Rodrigues Ribeiro, Caroline Marcantonio Ferreira, Maysa Mariana Cruz, Joice Naiara Bertaglia Pereira,et al.
Cells MDPI AG
Obesity is linked with altered microbial short-chain fatty acids (SCFAs), which are a signature of gut dysbiosis and inflammation. In the present study, we investigated whether tributyrin, a prodrug of the SCFA butyrate, could improve metabolic and inflammatory profiles in diet-induced obese mice. Mice fed a high-fat diet for eight weeks were treated with tributyrin or placebo for another six weeks. We show that obese mice treated with tributyrin had lower body weight gain and an improved insulin responsiveness and glucose metabolism, partly via reduced hepatic triglycerides content. Additionally, tributyrin induced an anti-inflammatory state in the adipose tissue by reduction of Il-1β and Tnf-a and increased Il-10, Tregs cells and M2-macrophages. Moreover, improvement in glucose metabolism and reduction of fat inflammatory states associated with tributyrin treatment were dependent on GPR109A activation. Our results indicate that exogenous targeting of SCFA butyrate attenuates metabolic and inflammatory dysfunction, highlighting a potentially novel approach to tackle obesity.


Impact of Dietary Fatty Acids on Macrophage Lipid Metabolism, Signaling and Function
Mariana Rodrigues Davanso, Amanda Rabello Crisma, Gilson Murata, Philip Newsholme, and Rui Curi
Ovid Technologies (Wolters Kluwer Health)
Abstract Leukocytes are potent regulators of adipose tissue biology and whole-body metabolic homeostasis. In lean, non-obese conditions (insulin-sensitive), adipose tissue has innate and adaptive immune cells, including eosinophils, regulatory T cells, invariant NK cells, and M2 macrophages. A vast expansion in adipose tissue occurs in obesity, and this is associated with a marked alteration in the tissue leukocyte profile. There is a marked increase in B cells, CD8+ T cells, NK cells, neutrophils, and M1 macrophages. This condition induces a state of low-grade, chronic inflammation in the adipose tissue, which disrupts whole-body metabolism. Macrophages were the first leukocyte to be discovered in adipose tissue. Due to their proximity to nearby adipocytes, the macrophages are exposed to high levels of fatty acids and other lipids reported in obesity. Lipid uptake by tissue-resident macrophages is essential for their biological actions. Specifically, lipid uptake and metabolism, particularly of long-chain saturated fatty acids, activate inflammatory signaling pathways, potentiating adipose tissue inflammation, and metabolic dysfunction. Obesity exhibits increased fatty acid levels within the adipose tissue microenvironment. The increased lipid accumulation in the resident macrophages reflects the fatty acid composition of the adipocytes. The dietary fatty acid determines the fatty acid composition of the adipose tissue. Macrophages then accumulate fatty acids indirectly provided by the diet. The composition varies with the acyl chain length, e.g., short-, medium-, or long-chain, and saturated fatty acids. These fatty acids have wide-ranging effects on macrophages. We described herein in detail the impact of the different dietary fatty acids on macrophage functions. Shortly, long-chain saturated fatty acids are pro-inflammatory, whereas medium-chain fatty acids are relatively benign. Long-chain unsaturated fatty acids often antagonize the pro-inflammatory effects of long-chain saturated fatty acids.


Maternal supplementation with a synbiotic has distinct outcomes on offspring gut microbiota formation in A/J and C57BL/6 mice, differentially affecting airway inflammatory cell infiltration and mucus production
Claudio Fukumori, Mateus B. Casaro, Andrew M. Thomas, Eduardo Mendes, Willian R. Ribeiro, Amanda R. Crisma, Gilson M. Murata, Bruna Bizzarro, Emmanuel Dias-Neto, Joao C. Setubal,et al.
Elsevier BV



Docosahexaenoic acid slows inflammation resolution and impairs the quality of healed skin tissue
Thamiris Candreva, Carolina M.C. Kühl, Beatriz Burger, Mariah B.P. dos Anjos, Márcio A. Torsoni, Sílvio R. Consonni, Amanda R. Crisma, Helena L. Fisk, Philip C. Calder, Felipe C.P. de Mato,et al.
Portland Press Ltd.
Abstract There is no consensus on the effects of omega-3 (ω-3) fatty acids (FA) on cutaneous repair. To solve this problem, we used 2 different approaches: (1) FAT-1 transgenic mice, capable of producing endogenous ω-3 FA; (2) wild-type (WT) mice orally supplemented with DHA-enriched fish oil. FAT-1 mice had higher systemic (serum) and local (skin tissue) ω-3 FA levels, mainly docosahexaenoic acid (DHA), in comparison with WT mice. FAT-1 mice had increased myeloperoxidase (MPO) activity and content of CXCL-1 and CXCL-2, and reduced IL-10 in the skin wound tissue three days after the wound induction. Inflammation was maintained by an elevated TNF-α concentration and presence of inflammatory cells and edema. Neutrophils and macrophages, isolated from FAT-1 mice, also produced increased TNF-α and reduced IL-10 levels. In these mice, the wound closure was delayed, with a wound area 6-fold bigger in relation with WT group, on the last day of analysis (14 days post-wounding). This was associated with poor orientation of collagen fibers and structural aspects in repaired tissue. Similarly, DHA group had a delay during late inflammatory phase. This group had increased TNF-α content and CD45+F4/80+ cells at the third day after skin wounding and increased concentrations of important metabolites derived from ω-3, like 18-HEPE, and reduced concentrations of those from ω-6 FA. In conclusion, elevated DHA content, achieved in both FAT-1 and DHA groups, slowed inflammation resolution and impaired the quality of healed skin tissue.


Adipose tissue is less responsive to food restriction anti-inflammatory effects than liver, muscle, and brain in mice
M.M. Antunes, C.B. de Almeida-Souza, G. Godoy, A.R. Crisma, L.N. Masi, R. Curi, and R.B. Bazotte
FapUNIFESP (SciELO)
High caloric intake promotes chronic inflammation, insulin resistance, and chronic diseases such as type-2 diabetes, which may be prevented by food restriction (FR). The effect of FR on expression of pro-inflammatory and anti-inflammatory genes in adipose tissue, liver, muscle, and brain was compared. Male Swiss mice were submitted to FR (FR group) or had free access to food (control group) during 56 days. The liver, gastrocnemius muscle, brain, and epididymal white adipose tissue (WAT) were collected for analysis of gene expressions. FR attenuated inflammation in the liver, brain, and gastrocnemius muscle but did not markedly change inflammatory gene expression in epididymal WAT. We concluded that adipose tissue was less responsive to FR in terms of gene expression of pro-inflammatory and anti-inflammatory genes.


Greater expression of postprandial inflammatory genes in humans after intervention with saturated when compared to unsaturated fatty acids
Milena Monfort-Pires, Amanda Rabello Crisma, Silvana Bordin, and Sandra Roberta G. Ferreira
Springer Science and Business Media LLC



Brain fatty acid composition and inflammation in mice fed with high-carbohydrate diet or high-fat diet
Lorena Gimenez da Silva-Santi, Marina Masetto Antunes, Marco Aurélio Mori, Camila Biesdorf de Almeida-Souza, Jesuí Vergílio Visentainer, Fabiana Carbonera, Amanda Rabello Crisma, Laureane Nunes Masi, Sandro Massao Hirabara, Rui Curi,et al.
MDPI AG
Both high fat diet (HFD) and high carbohydrate diet (HCD) modulate brain fatty acids (FA) composition. Notwithstanding, there is a lack of information on time sequence of brain FA deposition either for HFD or HCD. The changes in brain FA composition in mice fed with HFD or HCD for 7, 14, 28, or 56 days were compared with results of 0 (before starting given the diets). mRNA expressions of allograft inflammatory factor 1 (Aif1), cyclooxygenase-2 (Cox 2), F4/80, inducible nitric oxide synthase (iNOS), integrin subunit alpha m (Itgam), interleukin IL-1β (IL-1β), IL-6, IL-10, and tumor necrosis factor alpha (TNF-α) were measured. The HFD group had higher speed of deposition of saturated FA (SFA), monounsaturated FA (MUFA), and polyunsaturated FA (PUFA) at the beginning of the experimental period. However, on day 56, the total amount of SFA, MUFA, and PUFA were similar. mRNA expressions of F4/80 and Itgam, markers of microglia infiltration, were increased (p < 0.05) in the brain of the HCD group whereas inflammatory marker index (IMI) was higher (46%) in HFD group. In conclusion, the proportion of fat and carbohydrates in the diet modulates the speed deposition of FA and expression of inflammatory gene markers.


Attenuation of obesity and insulin resistance by fish oil supplementation is associated with improved skeletal muscle mitochondrial function in mice fed a high-fat diet
Amanda R. Martins, Amanda R. Crisma, Laureane N. Masi, Catia L. Amaral, Gabriel N. Marzuca-Nassr, Lucas H.M. Bomfim, Bruno G. Teodoro, André L. Queiroz, Tamires D.A. Serdan, Rosangela P. Torres,et al.
Elsevier BV



Palmitoleic acid (16:1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes
Maysa M. Cruz, Andressa B. Lopes, Amanda R. Crisma, Roberta C. C. de Sá, Wilson M. T. Kuwabara, Rui Curi, Paula B. M. de Andrade, and Maria I. C. Alonso-Vale
Springer Science and Business Media LLC



Prophylactic Bifidobacterium adolescentis ATTCC 15703 supplementation reduces partially allergic airway disease in Balb/c but not in C57BL/6 mice
M.C. Casaro, A.R. Crisma, A.T. Vieira, G.H.M. Silva, E. Mendes, W.R. Ribeiro, F.S. Martins, and C.M. Ferreira
Brill
Allergic asthma is a chronic disease mainly characterised by eosinophil inflammation and airway remodelling. Many studies have shown that the gut microbiota of allergic individuals differs from that of non-allergic individuals. Although high levels of bifidobacteria have been associated with healthy persons, Bifidobacterium adolescentis ATCC 15703, a gut bacteria, has been associated with allergic individuals in some clinical studies. The relationship between B. adolescentis ATCC 15703 and asthma or allergies has not been well elucidated, and its effect may be dependent on the host’s genetic profile or disease state. To elucidate this question, we evaluated the role of preventive B. adolescentis ATCC 15703 treatment on experimental allergic airway inflammation in two genetically different mouse strains, Balb/c and C57BL/6 (B6). Balb/c mice display a greater predisposition to develop allergic responses than B6 mice. Oral preventive treatment with B. adolescentis ATCC 15703 modulated experimental allergic airway inflammation, specifically in Balb/c mice, which showed decreased levels of eosinophils in the airway. B6 mice did not exhibit any significant alterations in eosinophils but showed an increased influx of total leukocytes and neutrophils into the airway. The mechanism underlying the beneficial effects of these bacteria in experimental allergic mice may involve products of bacteria metabolism, as dead bacteria did not mimic the ability of live B. adolescentis ATCC 15703 to attenuate the influx of eosinophils into the airway. To conclude, preventive oral B. adolescentis ATCC 15703 treatment can attenuate the major characteristic of allergic asthma, eosinophil airway influx, in Balb/c but not B6 mice. These results suggest that oral treatment with this specific live bacterial strain may have therapeutic potential for the treatment of allergic airway disease, although its effect is mouse-strain-dependent.


Combination of a high-fat diet with sweetened condensed milk exacerbates inflammation and insulin resistance induced by each separately in mice
Laureane Nunes Masi, Amanda Roque Martins, Amanda Rabello Crisma, Cátia Lira do Amaral, Mariana Rodrigues Davanso, Tamires Duarte Afonso Serdan, Roberta Dourado Cavalcante da Cunha de Sá, Maysa Mariana Cruz, Maria Isabel Cardoso Alonso-Vale, Rosângela Pavan Torres,et al.
Springer Science and Business Media LLC
AbstractObesogenic diets increase body weight and cause insulin resistance (IR), however, the association of these changes with the main macronutrient in the diet remains to be elucidated. Male C57BL/6 mice were fed with: control (CD), CD and sweetened condensed milk (HS), high-fat (HF), and HF and condensed milk (HSHF). After 2 months, increased body weight, glucose intolerance, adipocyte size and cholesterol levels were observed. As compared with CD, HS ingested the same amount of calories whereas HF and HSHF ingested less. HS had increased plasma AST activity and liver type I collagen. HF caused mild liver steatosis and hepatocellular damage. HF and HSHF increased LDL-cholesterol, hepatocyte and adipocyte hypertrophy, TNF-α by macrophages and decreased lipogenesis and adiponectin in adipose tissue (AT). HSHF exacerbated these effects, increasing IR, lipolysis, mRNA expression of F4/80 and leptin in AT, Tlr-4 in soleus muscle and IL-6, IL-1β, VCAM-1, and ICAM-1 protein in AT. The three obesogenic diets induced obesity and metabolic dysfunction. HS was more proinflammatory than the HF and induced hepatic fibrosis. The HF was more detrimental in terms of insulin sensitivity, and it caused liver steatosis. The combination HSHF exacerbated the effects of each separately on insulin resistance and AT inflammatory state.


Balanced diet-fed fat-1 transgenic mice exhibit lower hindlimb suspension-induced soleus muscle atrophy
Gabriel Nasri Marzuca-Nassr, Gilson Masahiro Murata, Amanda Roque Martins, Kaio Fernando Vitzel, Amanda Rabello Crisma, Rosângela Pavan Torres, Jorge Mancini-Filho, Jing Xuan Kang, and Rui Curi
MDPI AG
The consequences of two-week hindlimb suspension (HS) on skeletal muscle atrophy were investigated in balanced diet-fed Fat-1 transgenic and C57BL/6 wild-type mice. Body composition and gastrocnemius fatty acid composition were measured. Skeletal muscle force, cross-sectional area (CSA), and signaling pathways associated with protein synthesis (protein kinase B, Akt; ribosomal protein S6, S6, eukaryotic translation initiation factor 4E-binding protein 1, 4EBP1; glycogen synthase kinase3-beta, GSK3-beta; and extracellular-signal-regulated kinases 1/2, ERK 1/2) and protein degradation (atrophy gene-1/muscle atrophy F-box, atrogin-1/MAFbx and muscle RING finger 1, MuRF1) were evaluated in the soleus muscle. HS decreased soleus muscle wet and dry weights (by 43% and 26%, respectively), muscle isotonic and tetanic force (by 29% and 18%, respectively), CSA of the soleus muscle (by 36%), and soleus muscle fibers (by 45%). Fat-1 transgenic mice had a decrease in the ω-6/ω-3 polyunsaturated fatty acids (PUFAs) ratio as compared with C57BL/6 wild-type mice (56%, p < 0.001). Fat-1 mice had lower soleus muscle dry mass loss (by 10%) and preserved absolute isotonic force (by 17%) and CSA of the soleus muscle (by 28%) after HS as compared with C57BL/6 wild-type mice. p-GSK3B/GSK3B ratio was increased (by 70%) and MuRF-1 content decreased (by 50%) in the soleus muscle of Fat-1 mice after HS. Balanced diet-fed Fat-1 mice are able to preserve in part the soleus muscle mass, absolute isotonic force and CSA of the soleus muscle in a disuse condition.


Prophylactic supplementation of Bifidobacterium longum 5^1A protects mice from ovariectomy-induced exacerbated allergic airway inflammation and airway hyperresponsiveness
Eduardo Mendes, Beatriz G. Acetturi, Andrew M. Thomas, Flaviano dos S. Martins, Amanda R. Crisma, Gilson Murata, Tárcio T. Braga, Niels O. S. Camâra, Adriana L. dos S. Franco, João C. Setubal,et al.
Frontiers Media SA
Asthma is a chronic inflammatory disease that affects more females than males after puberty, and its symptoms and severity in women change during menstruation and menopause. Recently, evidence has demonstrated that interactions among the microbiota, female sex hormones, and immunity are associated with the development of autoimmune diseases. However, no studies have investigated if therapeutic gut microbiota modulation strategies could affect asthma exacerbation during menstruation and menopause. Here we aimed to examine the preventive effects of a probiotic, Bifidobacterium longum 51A, on airway inflammation exacerbation in allergic ovariectomized mice. We first evaluated the gut microbiota composition and diversity in mice 10 days after ovariectomy. Next, we examined whether re-exposure of ovariectomized allergic mice to antigen (ovalbumin) would lead to exacerbation of lung inflammation. Finally, we evaluated the preventive and treatment effect of B. longum 51A on lung inflammation and airway hyperresponsiveness. Our results showed that whereas ovariectomy caused no alterations in the gut microbiota composition and diversity in this animal model, 10 days after ovariectomy, preventive use administration of B. longum 51A, rather than its use after surgery was capable of attenuate the exacerbated lung inflammation and hyperresponsiveness in ovariectomized allergic mice. This prophylactic effect of B. longum 51A involves acetate production, which led to increased fecal acetate levels and, consequently, increased Treg cells in ovariectomized allergic mice.

RECENT SCHOLAR PUBLICATIONS

Evidence for a pro-inflammatory state of macrophages from non-obese type-2 diabetic Goto-Kakizaki rats
ASA Silveira, ACA Alves, GM Gimenes, PS Quessada, TB Lobato, ...
International Journal of Molecular Sciences 25 (19), 10240 2024



Evidence for a Pro-inflammatory State of Macrophages from Non-obese Type-2 Diabetic Goto-Kakizaki Rats
S ASdA, A ACdA, GM Gimenes, Q PdS, TB Lobato, BB Dias, ACG Pereira, ...
2024



Maternal low-protein diet reduces skeletal muscle protein synthesis and mass via Akt-mTOR pathway in adult rats
DAA de Vasconcelos, RT Nachbar, CH Pinheiro, CL do Amaral, ...
Frontiers in Nutrition 9, 947458 2022



Propolis authentication of stingless bees by mid-infrared spectroscopy and chemometric analysis
M Surek, A de Ftima Cobre, MM Fachi, TG Santos, R Pontarolo, ...
LWT 161, 113370 2022



The toxicity of Aspidosperma subincanum to MCF7 cells is related to modulation of oxidative status and proinflammatory pathways
AF Santos, NSRS Mota, EM Schiefer, RS da Cunha, AM Junkert, ...
Journal of Ethnopharmacology 281, 114512 2021



Microbiome-related differential effects of a probiotic on allergic airway inflammation
MB Casaro, A Thomas, E Mendes, C Fukumori, W Ribeiro, F Oliveira, ...
2021



Changes in PGC-1α-Dependent Mitochondrial Biogenesis Are Associated with Inflexible Hepatic Energy Metabolism in the Offspring Born to Dexamethasone-Treated Mothers
CV Campos, CJ Teixeira, TB Payolla, AR Crisma, GM Murata, AG Amaral, ...
Livers 1 (4), 201-220 2021



Immunomodulatory and cytotoxic activities of euphol
TL de Oliveira, L Bavia, PD Fontana, LS Cruz, KS Paludo, AR Crisma, ...
Life Sciences 280, 119700 2021



A probiotic has differential effects on allergic airway inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome
MB Casaro, AM Thomas, E Mendes, C Fukumori, WR Ribeiro, FA Oliveira, ...
Microbiome 9 (1), 134 2021



Chemical composition, cytotoxicity, and antibacterial activity of propolis from Africanized honeybees and three different Meliponini species
M Surek, MM Fachi, A de Ftima Cobre, FF de Oliveira, R Pontarolo, ...
Journal of Ethnopharmacology 269, 113662 2021



Effects of Euphorbia umbellata extracts on complement activation and chemotaxis of neutrophils
TL de Oliveira, PD Fontana, L Bavia, LS Cruz, AR Crisma, GL Sassaki, ...
Journal of Ethnopharmacology 265, 113348 2021



Macrophage inflammatory state in Type 1 diabetes: triggered by NLRP3/iNOS pathway and attenuated by docosahexaenoic acid
MR Davanso, AR Crisma, TT Braga, LN Masi, CL do Amaral, VNC Leal, ...
Clinical Science 135 (1), 19-34 2021



Schiefer counter: An alternative method for clonogenic assay evaluation
AF Santos, EM Schiefer, MC Atherino, JC Atherino, LH Negri, ...
Journal of Pharmacological and Toxicological Methods 106, 106911 2020



Tributyrin attenuates metabolic and inflammatory changes associated with obesity through a GPR109A-dependent mechanism
FT Sato, YA Yap, AR Crisma, M Portovedo, GM Murata, SM Hirabara, ...
Cells 9 (9), 2007 2020



Impact of dietary fatty acids on macrophage lipid metabolism, signaling and function
MR Davanso, AR Crisma, G Murata, P Newsholme, R Curi
Immunometabolism 2 (1), e200008 2020



Docosahexaenoic acid slows inflammation resolution and impairs the quality of healed skin tissue
T Candreva, CMC Khl, B Burger, MBP Dos Anjos, MA Torsoni, ...
Clinical Science 133 (22), 2345-2360 2019



Maternal supplementation with a synbiotic has distinct outcomes on offspring gut microbiota formation in A/J and C57BL/6 mice, differentially affecting airway inflammatory cell
C Fukumori, MB Casaro, AM Thomas, E Mendes, WR Ribeiro, AR Crisma, ...
Journal of Functional Foods 61, 103496 2019



Adipose tissue is less responsive to food restriction anti-inflammatory effects than liver, muscle, and brain in mice
MM Antunes, CB de Almeida-Souza, G Godoy, AR Crisma, LN Masi, ...
Brazilian Journal of Medical and Biological Research 52 (1), e8150 2019



Greater expression of postprandial inflammatory genes in humans after intervention with saturated when compared to unsaturated fatty acids
M Monfort-Pires, AR Crisma, S Bordin, SRG Ferreira
European journal of nutrition 57, 2887-2895 2018



Palmitoleic acid (16: 1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes
MM Cruz, AB Lopes, AR Crisma, RCC de S, WMT Kuwabara, R Curi, ...
Lipids in health and disease 17, 1-12 2018

MOST CITED SCHOLAR PUBLICATIONS

Tributyrin attenuates obesity-associated inflammation and insulin resistance in high-fat-fed mice
MAR Vinolo, HG Rodrigues, WT Festuccia, AR Crisma, VS Alves, ...
American Journal of Physiology-Endocrinology and Metabolism 303 (2), E272-E282 2012
Citations: 175



Hematological and biochemical reference values for C57BL/6, Swiss Webster and BALB/c mice
EW Santos, DC de OLIVEIRA, A Hastreiter, GB da SILVA, ...
Brazilian Journal of Veterinary Research and Animal Science 53 (2), 138-145 2016
Citations: 152



Both GLS silencing and GLS2 overexpression synergize with oxidative stress against proliferation of glioma cells
M Martn-Rufin, R Nascimento-Gomes, A Higuero, AR Crisma, ...
Journal of molecular medicine 92, 277-290 2014
Citations: 139



Attenuation of obesity and insulin resistance by fish oil supplementation is associated with improved skeletal muscle mitochondrial function in mice fed a high-fat diet
AR Martins, AR Crisma, LN Masi, CL Amaral, GN Marzuca-Nassr, ...
The journal of nutritional biochemistry 55, 76-88 2018
Citations: 83



Chemical composition, cytotoxicity, and antibacterial activity of propolis from Africanized honeybees and three different Meliponini species
M Surek, MM Fachi, A de Ftima Cobre, FF de Oliveira, R Pontarolo, ...
Journal of Ethnopharmacology 269, 113662 2021
Citations: 62



Fish oil prevents changes induced by a high‐fat diet on metabolism and adipokine secretion in mice subcutaneous and visceral adipocytes
RDCC de S, AR Crisma, MM Cruz, AR Martins, LN Masi, CL do Amaral, ...
The Journal of Physiology 594 (21), 6301-6317 2016
Citations: 57



Eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid differentially modulate rat neutrophil function in vitro
VA Paschoal, MAR Vinolo, AR Crisma, J Magdalon, R Curi
Lipids 48, 93-103 2013
Citations: 57



Palmitoleic acid (16: 1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes
MM Cruz, AB Lopes, AR Crisma, RCC de S, WMT Kuwabara, R Curi, ...
Lipids in health and disease 17, 1-12 2018
Citations: 56



Protein-energy malnutrition modifies the production of interleukin-10 in response to lipopolysaccharide (LPS) in a murine model
RA Fock, MAR Vinolo, AR Crisma, K Nakajima, MM Rogero, P Borelli
Journal of nutritional science and vitaminology 54 (5), 371-377 2008
Citations: 53



Sunflower oil supplementation has proinflammatory effects and does not reverse insulin resistance in obesity induced by high‐fat diet in C57BL/6 mice
LN Masi, AR Martins, JCR Neto, CL Amaral, AR Crisma, MAR Vinolo, ...
BioMed Research International 2012 (1), 945131 2012
Citations: 51



Iron bioavailability from ferric pyrophosphate in rats fed with fructan-containing yacon (Smallanthus sonchifolius) flour
AR Lobo, ML Cocato, P Borelli, EHS Gaievski, AR Crisma, K Nakajima, ...
Food Chemistry 126 (3), 885-891 2011
Citations: 51



Combination of a high-fat diet with sweetened condensed milk exacerbates inflammation and insulin resistance induced by each separately in mice
LN Masi, AR Martins, AR Crisma, CL do Amaral, MR Davanso, ...
Scientific reports 7 (1), 3937 2017
Citations: 49



Prophylactic Supplementation of Bifidobacterium longum 5^1A Protects Mice from Ovariectomy-Induced Exacerbated Allergic Airway Inflammation and Airway
E Mendes, BG Acetturi, AM Thomas, FS Martins, AR Crisma, G Murata, ...
Frontiers in microbiology 8, 1732 2017
Citations: 40



Macrophage inflammatory state in Type 1 diabetes: triggered by NLRP3/iNOS pathway and attenuated by docosahexaenoic acid
MR Davanso, AR Crisma, TT Braga, LN Masi, CL do Amaral, VNC Leal, ...
Clinical Science 135 (1), 19-34 2021
Citations: 37



DNA methylation changes induced by a high-fat diet and fish oil supplementation in the skeletal muscle of mice
CL Amaral, AR Crisma, LN Masi, AR Martins, SM Hirabara, R Curi
Lifestyle Genomics 7 (4-6), 314-326 2015
Citations: 36



Macadamia oil supplementation attenuates inflammation and adipocyte hypertrophy in obese mice
EA Lima, LS Silveira, LN Masi, AR Crisma, MR Davanso, GIG Souza, ...
Mediators of Inflammation 2014 (1), 870634 2014
Citations: 36



Tributyrin attenuates metabolic and inflammatory changes associated with obesity through a GPR109A-dependent mechanism
FT Sato, YA Yap, AR Crisma, M Portovedo, GM Murata, SM Hirabara, ...
Cells 9 (9), 2007 2020
Citations: 35



High-fat diet blunts activation of the nuclear factor-κB signaling pathway in lipopolysaccharide-stimulated peritoneal macrophages of Wistar rats
MC Borges, MAR Vinolo, AR Crisma, RA Fock, P Borelli, J Tirapegui, ...
Nutrition 29 (2), 443-449 2013
Citations: 32



Malnutrition suppresses cell cycle progression of hematopoietic progenitor cells in mice via cyclin D1 down-regulation
K Nakajima, AR Crisma, GB Silva, MM Rogero, RA Fock, P Borelli
Nutrition 30 (1), 82-89 2014
Citations: 31



Effects of high EPA and high DHA fish oils on changes in signaling associated with protein metabolism induced by hindlimb suspension in rats
GN Marzuca‐Nassr, KF Vitzel, LG De Sousa, GM Murata, AR Crisma, ...
Physiological reports 4 (18), e12958 2016
Citations: 27