Hptlc fingerprinting: A tool for simplified analysis of phenolics in medicinal plants Amit Yadaorao Saraf, Aparna Amit Saraf Indian Journal of Pharmaceutical Education and Research, 2020 Background: Phenolic compounds especially the flavonoids and tannins comprise one of the most significant classes of plant secondary metabolites. Aim: HPTLC is widely accepted by WHO and pharmacopoeia across the globe as an effective analytical method for the investigation of phenolics in herbal analyses. Materials and Methods: The methanolic extracts of Abrus precatorius L., Sapindus trifoliatus L. and Embelia ribes Burm. F. were developed on the HPTLC system to study the diversity of phenolic compounds like flavonoids and tannins under different development conditions. Flavanoids were separated using the combination of solvent system comprising of mid-polar to polar solvents, hence Ethyl acetate: formic acid: glacial acetic acid: water (10:0.5:0.5:1 v/v/v/v) solvent mixture and 10% methanolic Sulphuric Acid Reagent was used as spray reagent for separation of flavanoids. Tannins were separated on chromatogram by using the solvent system comprising of mid-polar to non-polar solvents, hence Toluene: Ethyl Acetate: Formic Acid (6:4:0.3 v/v/v) as solvent system and alcoholic FeCl3 was used for derivatization. Results: Distinct fingerprint of flavanoids and tannins was obtained. A profile of 11 to 12 polyvalent phytoconstituents were separated during the HPTLC analysis of flavonoids and tannins respectively. Conclusion: The HPTLC analysis successfully demonstrated that phenolic secondary metabolites can be effectively separated using the same extract under different development conditions. The detection and profiling of such metabolites provides the justification for therapeutic activities in medicinal plants.
Validation of HPTLC method for quantification of embelin from Embelia ribes Burm. F. International Journal of Pharmacognosy and Phytochemical Research, 2016
Phytochemical and elemental profile of Embelia ribes Burn. F Research Journal of Pharmaceutical Biological and Chemical Sciences, 2016
Phytochemical and antimicrobial studies of medicinal plant piper longum Linn International Journal of Pharmacognosy and Phytochemical Research, 2014
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MOST CITED SCHOLAR PUBLICATIONS
Phytochemical and Antimicrobial Studies of Medicinal Plant Piper longum Linn. AS Aparna Saraf International Journal of Pharmacognosy and Phytochemical Research 6 (2), 213-222 , 2014 2014.0 Citations: 22
Phytochemical Analysis and Chemical Fingerprinting of Seeds of Abrus precatorius L. ASAC AMIT SARAF Chemical Science Transactions 7 (1), 63-70 , 2018 2018.0 Citations: 21
Validation of HPTLC Method for Quantification of Embelin from Embelia ribes Burm. F. KSSAC Amit Saraf International Journal of Pharmacognosy and Phytochemical Research 8 (9 … , 2016 2016.0 Citations: 8
Pharmacognostic studies and HPTLC fingerprint profile of stem of Oroxylum indicum (L.) Vent: A threatened and vulnerable medicinal plant A Saraf, S Srinivas, NM Labhane Research Journal of Pharmacy, Biological and Chemical Sciences 4 (3), 1078-1084 , 2013 2013.0 Citations: 7
HPTLC Fingerprinting: A Tool for Simplified Analysis of Phenolics in Medicinal Plants SAA Saraf AY Indian J of Pharmaceutical Education and Research 54 (4), 1098-103 , 2020 2020.0 Citations: 6
Phytochemical and Elemental Profile of Embelia ribes Burn. F. KSSAC Amit Saraf RES J PHARM BIOL CHEM SCI 7 (4), 471-476 , 2016 2016.0 Citations: 5
Estimation Of Rutin From Morus Species Using Validated HPTLC Method AS Nikki Huria, Amit Saraf Int. J. of Pharm. Sci. 2 (3), 565-577 , 2024 2024.0 Citations: 4
Chromatographic Fingerprint Profile of Alkaloids of Abrus precatorius Linn. by HPTLC S Amit European Journal of Academic Essays 2 (2), 65-69 , 2015 2015.0 Citations: 4
Fast and simple HPTLC chemical fingerprinting and anti-oxidant analysis of medicinal herbs PAPSS AY Saraf, AA Saraf Journal of Pharmacognosy and Phytochemistry 13 (2), 806-809 , 2024 2024.0 Citations: 2
SCREENING OF CLASS OF PHYTOCHEMICAL COMPOUNDS AND ANTIOXIDANT AUTORADIOGRAPHIC EVALUATION IN PARIS POLYPHYLLA AND TRILLIUM GOVANIANUM USING HIGH-PERFORMANCE THIN-LAYER … R Rashid, U Yadav, N Labhane, A Saraf World Journal of Pharmaceutical Research 15 (2), 948-957 , 2026 2026.0
PHYTOCHEMICAL ANALYSIS AND CHEMICAL FINGERPRINTING OF EMBELIA RIBES BURM. F. AS AlkaChaturvedi IJRBAT 6 (3), 36-38 , 2018 2018.0
An In-Vitro Evaluation of Flowers of Tagetus Spp.(L) as A Natural Anti-Solar Agent TDDSAY Saraf Aparna A Nature and Science 8 (10), 274-276 , 2010 2010.0
Phytochemical Studies and evaluation of antibacterial activity of Zanthoxylum budrunga DC Wall. AS Aparna Saraf Bionano Frontiers, 21-23 , 2009 2009.0
Research Journal of Pharmaceutical, Biological and Chemical Sciences A Saraf, KS Srinivas, A Chaturvedi
