Holds a Bachelor's degree in Biological Sciences from the State University of Maringá (1992), a Master's degree in Biological Sciences (Cell Biology) from the State University of Maringá (1996), and a PhD in Biological Sciences (Cell Biology with a focus on cytogenetics) from the State University of Maringá (2008). Currently, they are an associate professor at the State University of Maringá teaching the subject of General Pathology. Has experience in the field of General Pathology, with an emphasis on experimental histopathology, mainly acting on the following topics: Carcinogenesis - induction of dysplasias (aberrant crypts) and neoplasms in the intestinal colon through the inoculation of chemical carcinogens and evaluation of natural antimutagenic/antioxidant products in vivo, aimed at chemoprevention of breast and colorectal cancer. Utilization of cytogenetic techniques, immunohistochemical techniques for identifying histopathological alterations, and molecular techniques (RT-PCR).
EDUCATION
Degree in biological sciences
Master's Degree in Biological Sciences (Cell Biology)
PhD in Biological Sciences (Cell Biology)
RESEARCH, TEACHING, or OTHER INTERESTS
Public Health, Environmental and Occupational Health, Toxicology, Multidisciplinary, Multidisciplinary
13
Scopus Publications
Scopus Publications
Evaluation of antineoplasic activity of Zingiber officinale essential oil in the colorectal region of wistar rats Daniel Augusto Nunes de Lima, Brenda Barroso Pelegrini, Felipe Alexandre Alves Uechi, Raíssa Coracini Varago, Bruno Bueno Pimenta, et al. Asian Pacific Journal of Cancer Prevention, 2020 Introduction: Aberrant Crypt (AC) and Aberrant Crypt Focus (ACF) are considered pre-neoplasic lesions, ranging from hyperplasia to different degrees of dysplasia in the colon. This work aimed to evaluate and quantify the chemopreventive activity of Zingiber officinale essential oil in the colorectal region of Wistar rats. Materials and Methods: We extracted the essential oil from ginger rhizomes and carried out ACF induction, in rats, with 1.2 Dimethylhydrazine (DMH) at a 20 mg/kg dose. The experimental groups were GI (negative control); GII (positive induction control); GIII (DMH + essential oil); GIV (DMH +5-Florouracil) and GV (essential oil). The histological techniques used were methylene blue, hematoxylin-eosin (HE) dyeing, and immunohistochemistry (IHQ). Results: The major essential oil compounds were citral (17.25%), δ-citral (10.25%), camphene (9.55%), α-zingiberene (7.57%), nerol (6.37%) and plelandrene (6.83%). For the presence of AC or ACF, we did not observe them in GI and GV, while in GII and GIII, they were observed, in high values, in both regions, but only in the distal region, there was a significant difference between them. For GIV, for both regions, there were significant lower numbers of AC when compared to GIII. As observed, with HE, there were hyperplastic and dysplastic ACF in the proximal and distal portions of the colon. For IHQ analyses, there were positively PCNA antibody marked cells in all experimental groups. Yet, there was no significant correlation of mitotic index among them. Moreover, the results of GIII compared to GIV were very similar. Conclusion: In this sense, the Zingiber officinale essential oil has good antioxidant potential because it presents a mixture of monoterpene and sesquiterpene compounds. Thus, it is able to develop a chemoprotective effect, as it presented similar results to the standard drug, showing cell proliferation control.
Rosuvastatin reduced brain parasite burden in a chronic toxoplasmosis in vivo model and influenced the neuropathological pattern of ME-49 strain L. Nishi, P. L. Santana, F. F. Evangelista, L. F. Beletini, A. H. Souza, et al. Parasitology, 2020 This study evaluated the effects of rosuvastatin in vivo on toxoplasmosis chronic infection. Thirty-five Swiss mice were orally infected (ME-49 strain). After 50 days, the mice were separated into five groups: GI – non-infected, GII – infected, GIII – infected and treated with pyrimethamine and sulfadiazine (12.5 + 50 mg kg−1 body weight day−1), GIV and GV – infected and treated with rosuvastatin 10 and 40 mg kg−1 body weight day−1, respectively. After 21 days, we collected blood, liver, lungs, femoral biceps and brain were removed for Toxoplasma gondii DNA quantification by qPCR and histopathological analysis. GIV and GV did not present premature death or clinical changes, and the hepatic enzyme levels were lower compared to GI. Toxoplasma gondii DNA was detected mainly in brain and muscle, but the parasite load was significantly lower in GV compared to GII brains (P < 0.05). Histopathological changes were observed in brains, with T. gondii cysts as well as an inflammatory condition, including necrosis areas in GII and GIII. These data confirm active infection with tissue injury. This inflammatory condition was attenuated in the groups treated with rosuvastatin, especially R40 (GV). Our findings demonstrated the in vivo action of rosuvastatin in reducing cerebral parasitic load and indicate that this drug may interfere in chronic toxoplasmosis.
Factors related to the painful conditions of patients with fibromyalgia in Maringá, Paraná State, Brazil Jean Paulo Coelho Leal, Isabela Maria Bertoglio, Lygia Queiroz Esper, Alice Maria de Souza Kaneshima, Tania Cristina Alexandrino Becker, et al. Acta Scientiarum Health Sciences, 2020 This study aimed to obtain personal and clinical characteristics of patients with fibromyalgia, and to identify the factors responsible for the initiation, recurrence or intensification of painful state in each patient. The information was collected using General Characterization Form of Fibromyalgia Patients, consisting of 32 questions about personal data, location, and type of pain, identification of factors or circumstances related to the algetic state, physical activity, and pharmacological treatment. Among 14 patients included in this study, 85.71% were female. The pain intensity and time spam varied among patients, and 42.85% of them presented pain (algetic state) lasting between 6 to 10 years. Emotional trauma was the factor or circumstance related to the onset of symptoms of 71.43% of the patients. All patients reported that stressful situations resulting from conflicts in the family, social or work environment are factors responsible for reactivating and intensifying the symptoms of fibromyalgia. Factors and circumstances related to the onset, recurrence, and intensity of fibromyalgia symptoms reveal that it is necessary to inform patients that it is a benign disease, despite the painful condition,since the way they respond to daily events is a determining factor in the course of the disease.Improving physical conditioning and conducting cognitive-behavioral therapy, along with pharmacological treatments, can be successful in treating or minimizing the symptoms of fibromyalgia.
Meiotic behavior in Panicum maximum Jacq. (Poaceae: Panicoideae: Paniceae): Hybrids and their genitors Maria Suely Pagliarini, Cleide Pessim, Liana Jank, Alice Maria de Souza Kaneshima, Andréa Beatriz Mendes Bonato Acta Scientiarum Agronomy, 2010 Panicum maximum Jacq. is one of the most cultivated grasses in the world. The intraspecific hybridization breeding program underway at Embrapa Beef Cattle aims at combining several agronomic characteristics in a single plant with high seed production. Four intraspecific hybrids originated from the cross between sexual tetraploid female plants with apomictic tetraploid male plants, including the genitors, were cytologically evaluated in the meiotic process throughout conventional methodology. Hybrids and genitors showed high meiotic stability. The percentage of abnormal cells among them ranged from 6.7 to 14.2%. Abnormalities recorded were irregular chromosome segregation, chromosome stickiness, and absence of the first cytokinesis, but the latter did not compromise pollen viability. Multivalent chromosome configurations at diakinesis, ranging from one to three quadrivalents, suggest that genetic recombination and introgression of some genes can be expected in the hybrids. Based on meiotic stability, the hybrids should be selected for high seed production, and the genitors could remain in the program participating in other intraspecific crosses.
Meiotic behaviour in the first interspecific hybrids between Brachiaria brizantha and Brachiaria decumbens A. M. De Souza‐Kaneshima, C. Simioni, M. F. Felismino, A. B. Mendes‐Bonato, C. Risso‐Pascotto, et al. Plant Breeding, 2010 With 2 figures and 1 table Abstract Brachiaria brizantha and Brachiaria decumbens constitute the two most widely used tropical forage species for cultivated pastures and support both beef and dairy cattle production in the tropics. Two apomictic cultivars –B. brizantha cv. ‘Marandu’ and B. decumbens cv. ‘Basilisk’– cover more than 100 million hectares of cultivated pastures throughout Latin America and Southeast Asia. This paper discusses the cytogenetic data of the first successful attempt in obtaining interspecific hybrids between these two species. Artificial hybridization between two diploid (2n = 2x = 18) sexual accessions (B. brizantha, B105 × B. decumbens, D004) was performed in the greenhouse. Only three hybrids were recovered. One of them was treated with colchicine in tissue culture and two amphidiploid plants were obtained. Meiosis in the diploid genitors was quite normal, while in the diploid hybrid parental genome separation was observed together with some irregular chromosome segregation. Genome separation was also observed in several cells in the two 4n amphidiploid hybrids. In these, a high number of meiocytes with chromosomes chaotically spread in the cytoplasm was recorded during meiosis. The abnormalities observed compromised the meiotic products characterized by polyads. Chromosomes associated generally as bivalents but some association as quadrivalents in diakinesis in one of the amphidiploid hybrids indicates taxonomic proximity between these two species. Moreover, it indicates that introgression of desirable genes may be feasible between these two most important agronomic species of the genus.
Identifying body image disorders and behaviors leading to the development of bulimia nervosa in adolescents from a public high school in Marangá, Paraná state Alice Maria de Souza-Kaneshima, Angela Andréia França, Daniele De Pinto Freitas Kneube, Edilson Nobuyoshi Kaneshima Acta Scientiarum Health Sciences, 2008 Conscious of the importance of studying eating disorders in adolescents, this work identified the onset of body image disorders and bulimia nervosa in 187 adolescents. Using the Body Shape Questionnaire (BSQ), it was shown that 48.13% of adolescents displayed body image disorders. The application of the Bulimic Investigatory Test, Edinbugh (BITE) demonstrated that 3.74 and 39.04% of adolescents presented a high or medium level of eating disorder, respectively. In the subscale of BITE severity, it was detected that 2.67 and 7.49% of adolescents showed high and moderated gravity of bulimia nervosa. The results revealed some adolescents with attitudes and behaviors that favor the development of bulimia nervosa, due to a distorted perception of their body image. Therefore, educational campaigns are necessary to clarify that the cult of the body is associated with serious eating disorders.
Occurrences of nervous anorexy and body image disorder in middle-school students in the city of Maringá, State of Paraná Acta Scientiarum Health Sciences, 2006
