Development of animal model for studying deep second-degree thermal burns Danielle dos Santos Tavares Pereira, Maria Helena Madruga Lima-Ribeiro, Nicodemos Teles de Pontes-Filho, Ana Maria dos Anjos Carneiro-Leão, Maria Tereza dos Santos Correia Journal of Biomedicine and Biotechnology, 2012 Thermal lesions were produced in 12 male Wistar rats, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99°C ± 2°C/10 min. on the back of each animal for 15 sec. After 7, 14, 21, and 28 days, animals were euthanized. The edema intensity was mild, with no bubble and formation of a thick and dry crust from the 3rd day. The percentage of tissue shrinkage at 28 days was 66.67 ± 1.66%. There was no sign of infection, bleeding, or secretion. Within 28 days reepithelialization was incomplete, with fibroblastic proliferation and moderate fibrosis and presence of modeled dense collagen fibers. It is concluded that the model established is applicable in obtaining deep second-degree thermal burns in order to evaluate the healing action of therapeutic agents of topical use.
Topical application effect of the isolectin hydrogel (Cramoll 1,4) on second-degree burns: Experimental model Danielle dos Santos Tavares Pereira, Maria Helena Madruga Lima-Ribeiro, Ralph Santos-Oliveira, Carmelita de Lima Bezerra Cavalcanti, Nicodemos Teles de Pontes-Filho, et al. Journal of Biomedicine and Biotechnology, 2012 This study aimed at evaluating the use of hydrogel isolectin in the treatment of second-degree burns. Twenty male rats were randomly divided into two groups (G1 = treatment with hydrogel containing 100 μg/mL Cramoll 1,4 and G2 = Control, hydrogel). After 7, 14, 21, 28, and 35 days, animals were euthanized. On the 7th day, G1 showed intense exudates, necrosis and edema. On the 14th day, G1 showed tissue reepithelialization and moderate autolysis. On the 21st day, G1 showed intense fibroblastic proliferation, presence of dense collagen, and moderate fibrosis. On the 28th day, G1 showed complete tissue epithelialization. On the 35th day, G1 showed modeled dense collagen. The significant wound contraction was initiated from day, 14 in the G1. There were no significant differences in biochemical and hematological parameters analyzed. These results extend the potential of therapeutic applications for Cramoll 1,4 in the treatment of thermal burns.
Radiolabeling of cramoll 1,4: Evaluation of the biodistribution Beatriz Ferreira de Carvalho Patricio, Maria Helena Madruga Lima-Ribeiro, Maria Tereza dos Santos Correia, Ana Maria dos Anjos Carneiro-Leão, Marta de Souza Albernaz, et al. International Journal of Peptides, 2011 The cramoll 1,4 is a well-studied lectin. However, few studies about its biodistribution have been done before. In this study, we radiolabeled the cramol 1,4 with Tc-99m and analyzed the biodistribution. The results showed that the cramol has an abnormal uptake by the bowel with reflections on its clearance mechanism.