Andi Hamdillah
@umi.ac.id
Aquaculture
universitas muslim indonesia
RESEARCH INTERESTS
Antibacterial, Fish Pathogen
Scopus Publications
Scopus Publications
Harlina Harlina, Rosmiati Rosmiati, Andi Hamdillah, Syahrul Syahrul, and Yosie Andriani
The Korean Society of Fisheries and Aquatic Science
The efficacy of Chromolaena odorata leaf flour components (CO) to increase the growth performance, non-specific immune activity, and resistance against Vibrio parahaemolyticus of whiteleg shrimp ( Litopenaeus vannamei ) culture was evaluated. To this purpose, whiteleg shrimp post larvae were fed on diets supplemented with 0 and a 1.5 g CO/kg diet for 45 days in a pond. After feeding trials, the shrimps were identified for their growth parameters, collected, and injected with V. parahaemolyticus , then their non-specific immune activity and resistance to V. parahaemolyticus was observed for 14 days. Findings showed that CO increased the average body weight (7.71 g, 37%), weight gain (7.69 g, 40%), and specific growth rate (13.23%/day, 5.7%) as compared to the control. In addition, CO supplementation also increases shrimp’s hematologic and immune activity (total hemo-cyte counts [6.8 × 10 7 CFU/mL, 242.9%], differential hemocyte counts [27%, 142.1%], and prophenoloxidase activity [0.085%, 566.7%]). Finally, shrimp resistance to V. parahaemolyticus infection also increased after CO supplementation, with survival rates of 73.33% as compared to 23.33% for the control. It suggests that C. odorata leaf flour component supplementation at an optimal dose in the diet may be an effective strategy to increase growth and resistance to bacterial disease with reduced mortality in shrimp farms.
Rustam, Jayadi, Andi Hamdillah, and Nursyahran
Insight Society
Barong shrimp or lobster (Panulirus versicolor) is a fishery commodity with high economic value and great demand. This study aims to determine the genetic characteristics of Panulirus versicolor at the fishing location in the waters of Barrang Caddi Island, Makassar Strait, using primers LCO-1490F and HCO-2198R. Genomic DNA extraction was performed using a Quick-DNA Tissue Miniprep Kit for total isolation. For DNA purity, the elution buffer utilized in this research was specifically selected for its suitability for PCR amplification with KOD FX Neo, providing optimal conditions for efficient genetic analysis. The primary genes used were LCO-1490F with primers GGT CAA CAA ATC ATA AAG ATA TTG G, and HCO-2198R with TAA ACT TCA GGG TGA CCA AAA AAT CA. The study utilized a 1% agarose gel in a 1X TBE buffer at 50 voltage for 45 minutes to move the total DNA and PCR products. The analysis results at sampling stations 0.2-03 showed increasingly smaller genetic distances. Based on the results of BLASTn analysis, each station had the same type of Panulirus versicolor with a similarity of 99-100%. The morphological characteristics included a carapace with a black spot and black and white lines on each abdominal segment. The analysis of genetic variation in the lobster population on Barrang Caddi Island using primers LCO-1490F and HCO-2198R obtained a DNA fragment of 700 bp. The population of Panulirus versicolor lobster on Barrang Caddi Island showed a high level of similarity or low diversity.
Harlina Harlina, Andi Hamdillah, Kamaruddin Kamaruddin, and Siti Aslamyah
IOP Publishing
Copra meal is an alternative protein source as fish meal in fish feed because the nutritional value is almost the same. However, copra meal has a relatively high crude fiber content, so efforts are needed to reduce crude fiber content through the fermentation process using the microbe Rizopus sp. which produces cellulase enzymes. This study aims to determine the digestibility level of fermented copra meal in feed as a source of vegetable protein in the maintenance of saline tilapia (Oreochromis niloticus) seeds. The research were conducted with the addition of fermented, dried and powdered copra meal to the feed with a concentration of: (A) 0%; (B) 15%; (C) 30% and (D) 45%. The fish were given test feed on a daily basis with a feeding frequency of once per day. The results showed that giving 15% of the fermented copra meal as plant protein source in the feed gave a better total feed digestibility compared to the addition of unfermented copra meal.
Andi Hamdillah, Alim Isnansetyo, Indah Istiqomah, Indun Dewi Puspita, Desy Putri Handayani, and Takushi Kaneko
EManuscript Services
Coastal plants and sponges are marine resources that have been widely known as producers of bioactive compounds.1-3 They are rich sources of alkaloids, saponins, tannins, flavonoids, terpenoids, and glycosides1,4 many of which exhibit various bioactivities including anticancer,5 antifungal,2,3,6 antiviral,4 antioxidant7-9 and antibacterial activities.2,4,6,8,10,11 The application of antibacterial compounds from coastal plants and sponges are not only limited in medication of disease caused by human pathogenic bacteria12,13 but also have been applied to overcome the problem of fish bacterial infections.2,12