Preparation and Characterization of P(3HB-co-HHX)/Silk Fibroin Nanofiber for Chondrogenic Differentiation on Human Adipose Mesenchymal Stem Cell Andri Pramesyanti Pramono, Retno Yulianti, Muhamad Alif Razi, Karina, Shaik Ling Ang, Kumar Sudesh, Hanan, Yuni Cahya Endarwati, Muhammad Radian Ghoza Hayati Journal of Biosciences, 2026 This study explored the proliferative and chondrogenic differentiation capacities of nanofiber containing silk fibroin hybrid Bombyx mori silkworm cocoons (Japan-China SP-01 variant from Indonesia) and PHA P(3HB-co-3HHx) on human adipose mesenchymal stem cells. First, the scaffolds were prepared for electrospinning by combining two distinct biomaterials, consisting of silk fibroin derived from hybrid Bombyx mori silkworm cocoons, with a combination of 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx). The effects of various ratios of P(3HB-co-3HHx)/Silk Fibroin nanofiber mixture on proliferative and differentiation capacity were then investigated. Following that, the morphology, chemical compositions, contact angle, tensile strength, roughness, cell viability, and human adipose mesenchymal stem cell differentiation of the nanofiber were investigated by collagen type 2 gene expression. The results of scanning electron microscopy showed the mean diameter of the nanofiber ranged from 370-600 nm. Following that, 16,000 volts was prominent for nanofiber manufacture in all ratios. It was also demonstrated that the nanofiber has significant mechanical properties, acceptable hydrophilicity and smoothness, and appropriate cell viability (up to 99.1% compared to the control on silk fibroin nanofiber). Although PHA increased tensile strength, silk fibroin administration to the mixture predominantly enhanced chondrogenic differentiation, as evidenced by modulation of chondrogenic collagen type 2 (up to 8.718-fold) gene markers. Furthermore, the physicochemical characteristics of the nanofiber mixture significantly influenced the proliferation and differentiation of human adipose mesenchymal stem cells. The results of the tests showed that silk fibroin administration into a nanofiber mixture has improved chondrogenesis and showed great potential as a cartilage tissue scaffold.
Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf Extract Uswatun Hasanah, Cut Fauziah, Nasihin saud Irsyad, Andri Pramesyanti Pramono, Dinda Hanifah Wahab, Wafa Nabila Nawawi Hayati Journal of Biosciences, 2025 Spermatogenesis is the process of spermatozoa formation. An increase in free radicals, such as in hyperglycemia conditions, causes oxidative stress, which can interfere with spermatogenesis. Oxidative stress is an inequality condition between the body’s capacity to produce antioxidants and the rise of free radicals. Antioxidants such as flavonoids are contained in plants such as Moringa oleifera leaves. During spermatogenesis, an apoptosis process involves the BAX gene. BAX is a gene that functions as pro-apoptosis. There is also a CO1 gene in mitochondria, which plays a role in cellular metabolism. Gene expression examination assesses the comparative threshold cycle (CT) with RT-PCR, and spermatozoa quality examination includes assessing spermatozoa concentration and motility. This study used 30 male white Sprague Dawley rats as the test animals, which were divided into five treatment groups: treatment of moringa leaf extract dose 200 mg/kg BW, treatment dose 300 mg/kg BW, treatment dose 400 mg/kg BW, normal control group, and positive control group. The results showed a decrease in BAX gene expression and CO1 gene expression in the treatment group of test animals. The results of sperm analysis showed an increase in spermatozoa concentration in all treatment groups compared to the control group. For sperm motility in the 400 mg/KgBB dose treatment, an increase in sperm motility compared to the control group. This study concludes that administering moringa leaf extract reduced the expression of the Bax and CO1 genes. However, no significant changes were observed in sperm concentration and motility within the treatment group.
Implication of taxonomic abundance of gut microbiota in prediabetes: a systematic review Iche Andriyani Liberty, Laily Hanifah, Hanna Farida Rachmat, Aidi Alifia Putri, Dessy Pratiwi, Andri Pramesyanti Pramono, Fachmi Idris, Suryadi Tjekyan Frontiers in Nutrition, 2025 BackgroundPrediabetes is defined by blood glucose levels that are higher than normal but below the diagnostic threshold for diabetes. Environmental factors associated with diabetes may contribute to its development through alterations in the gut microbiota. Recent studies suggest that changes in the composition and function of the gut microbiota play a role in the pathogenesis of diabetes mellitus and other metabolic disorders.ObjectiveThis study aims to systematically examine taxonomic abundance and its implications in the gut microbiota of individuals with prediabetes, identify key dysbiotic patterns, and explore their potential role in inflammation, insulin resistance, and progression to type 2 diabetes.MethodsWe conducted a systematic literature review following PRISMA guidelines. The review included sources from PubMed, ClinicalKey, ScienceDirect, Springer, and Scopus. We retrieved original research articles published in English that focused on prediabetes and gut microbiota from 2015 to the date of our search. Out of 827 full-text articles screened, 6 were selected based on defined inclusion and exclusion criteria.ResultsDysbiosis of the gut microbiota in prediabetes is characterized by a reduction in butyrate-producing bacteria such as Faecalibacterium prausnitzii and Roseburia, along with an increase in potentially harmful taxa such as Escherichia/Shigella and Prevotella species. This imbalance is associated with systemic inflammation and insulin resistance, evidenced by elevated levels of C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and lipopolysaccharide-binding protein (LBP). Increased intestinal permeability facilitates the translocation of bacterial components such as lipopolysaccharides (LPS), further linking gut microbiota changes to the development of insulin resistance and type 2 diabetes.ConclusionThis review highlights the need for further research to explore the potential therapeutic role of gut microbiota in prediabetes.Systematic Review RegistrationProspero; Identifier CRD42025637369.
Exploring the Role of Buckwheat-Derived SCFAs in Modulating Gut Microbiota-Immune Interactions in Metabolic Syndrome Sri Desfita, Wulan Sari, Winda Septiani, Fachriani Putri, Yusmarini Yusmarini, et al. Current Nutrition and Food Science, 2025 Background: Metabolic syndrome has been recognized as a significant public health issue closely related to dysbiosis and systemic inflammation, which are influenced by gut microbiota and its metabolites. Objective: This review focuses on the Short-Chain Fatty Acids (SCFAs) from buckwheat in modifying gut microbiota and immune response to combat metabolic syndrome. We provide an overview of studies on the substantial function of buckwheat-derived SCFAs in the interaction between gut microbiota and the immune system, particularly in metabolic syndrome. Methods: A detailed literature review was performed from 2015 to 2025, utilizing PubMed, ScienceDirect, SpringerLink, and Google Scholar databases. The data was analyzed descriptively and systematically. Results and Discussion: Buckwheat's resistant starch, polyphenols, and bioactive peptides promote SCFA production, reduce chronic inflammation, and lower the risk of metabolic syndromes. However, the heterogeneity in gut microbiota response among subjects underlines the relevance of individualized nutrition. Future research should prioritize large-scale trials to validate these findings and examine long-term impacts. Conclusion: The function of SCFAs produced by buckwheat as an alternative functional food to mitigate metabolic syndrome was explored in this review.
Synergistic Effects of Multi-Strain Probiotic and Prebiotic Combinations on Immune Recovery in Aging Populations Sri Desfita, Wulan Sari, Denai Wahyuni, Fachriani Putri, Andri Pramesyanti Pramono, Usman Pato, Dessy Pratiwi, Joanna Grzelczyk, Grazyna Budryn International Journal of Probiotics and Prebiotics, 2025 Aging populations have significant challenges related to immune system decline, known as immunosenescence, exacerbated by gut microbiota imbalances. This review explores the synergistic effects of multi-strain probiotics and prebiotics, known as synbiotics, on immune restoration in aging individuals. The aim is to synthesize current evidence on these combinations’ mechanisms, clinical outcomes, and therapeutic potential. The methodology involved a comprehensive review of the literature from 2014 to 2025, examining the impact of probiotics, prebiotics, and their combinations on gut microbiota diversity, immune modulation, and inflammation. The results and discussion were presented descriptively and systematically. Multi-strain probiotics enhance gut microbiota diversity, increase anti-inflammatory cytokines, and promote short-chain fatty acid production, which supports gut barrier integrity. Prebiotics selectively stimulate beneficial microbes, fostering an optimal environment for probiotic efficacy. Together, synbiotics demonstrate superior outcomes, including significant reductions in inflammation, improved gut barrier function, and enhanced systemic immunity. However, individual responses and long-term safety remain areas for further investigation. In conclusion, synbiotics represent a transformative approach to immune recovery in aging populations, offering benefits beyond gut health. Future research should prioritize personalized interventions and standardized protocols to optimize their therapeutic potential.
Differences in Treatment of Royal Jelly Powder by Freeze-drying as a Serum Substitute in Media Culture for Lymphocyte Cell Pratiwi Rostiningtyas Lusiono, Muhamad Sahlan, Andri Pramesyanti Pramono, Diah Kartika Pratami, Sabar Pambudi, Retno Wahyu Nurhayati Aip Conference Proceedings, 2024 Fetal Bovine Serum (FBS), which offers nutrients for cell growth, has been the most widely used serum in proliferation studies. It does, however, necessitate screening because it contains unidentified elements, as well as viruses and prions, which pose a risk of infection. Apis mellifera royal jelly has the potential to replace FBS as a serum. In this study, a comparison of the effectiveness of three royal jelly treatments on lymphocyte cell proliferation will be performed: untreated royal jelly, soluble royal jelly, and hydrolyzed royal jelly, which is then freeze-dyed to generate a powdered product. Lymphocyte cells were cultured with various concentrations (2.5%, 5%, 7.5%, and 10%) and three different treatments of royal jelly. The results of these various concentrations were continued for up to 48 hours with continuous checking every 24 hours measured by the Microtetrazolium (MTT) assay. According to the results, lymphocyte cells cultured with the addition of a 2.5% concentration of untreated royal jelly Apis mellifera had significant differences, with the percent cell viability of 77.15% at 24 hours and 58.44% at 48 hours. This value was higher than that of soluble royal jelly (64.13% and 38.52%) and royal jelly hydrolyzate (71.08% and 54.59%) with the addition of the same concentration.
Genetic variations and relationship among dengue virus type 3 strains isolated from patients with mild or severe form of dengue disease in Indonesia and Thailand Southeast Asian Journal of Tropical Medicine and Public Health, 2005
