Anne-Sophie Hascoet

@mpaveterinary.com

MPA Veterinary Medicines and Additives

https://researchid.co/annesophieh

RESEARCH, TEACHING, or OTHER INTERESTS

Veterinary, Food Animals, Food Science

Scopus Publications

385

Scholar Citations

Scholar h-index

Scholar i10-index

Scopus Publications

Evaluation of the Effectiveness of a Phytogenic Supplement (Alkaloids and Flavonoids) in the Control of Eimeria spp. in Experimentally Challenged Broiler Chickens
Anne-Sophie Hascoët, Paulina Torres-Celpa, Roberto Riquelme-Neira, and Héctor Hidalgo-Olate
MDPI AG
Avian coccidiosis remains a problematic challenge in poultry farms worldwide, with increasing concerns about resistance to conventional anticoccidial drugs, highlighting the need for alternative control strategies. A total of 256 male broilers (Ross 308), 1 day old, were randomly assigned to four experimental groups (eight replicates, each containing eight chickens) to evaluate the effectiveness of a phytogenic supplement against Eimeria spp. At 14 days, broilers were challenged via oral gavage with a mixture of six strains of live Eimeria spp. oocysts (4.0 × 104 Eimeria spp. oocysts per bird). The groups included a negative control (no anticoccidials or challenge), a positive control (coccidial challenge, no anticoccidials), a phytogenic-supplemented diet based on alkaloids and flavonoids, and a diet with anticoccidials (narasin and nicarbazin). Fecal samples were collected for oocyst counts from day 14 to 23, and at 23 days (9 days post-infection), intestinal lesions and mucosa measurements were evaluated. The oocyst counts in the phytogenic and anticoccidial groups were significantly lower than in the positive control (with a reduction of 61.3% and 71.6%, respectively, compared to the positive control) (p < 0.05), with no significant differences between the phytogenic supplement and anticoccidials. The phytogenic supplement provided intermediate protection based on histopathological scores with a significantly lower histopathology score than the positive control but significantly higher than the negative control (p < 0.05). No differences in body weight, feed intake, feed conversion ratio, or mortality were observed across groups during the trial, likely due to the moderate challenge applied. In conclusion, the phytogenic supplement demonstrated anticoccidial activity comparable to narasin and nicarbazin without compromising productivity, warranting further research into its mechanisms, resistance impact, and commercial application.

Effectiveness of enzymatic treatment for reducing dairy fouling at pilot-plant scale under real cleaning conditions
Alfons Eduard Guerrero-Navarro, Abel Guillermo Ríos-Castillo, Carolina Ripolles-Avila, Anna Zamora, Anne-Sophie Hascoët, Xavier Felipe, Manuel Castillo, and José Juan Rodríguez-Jerez
Elsevier BV

In vitro preformed biofilms of bacillus safensis inhibit the adhesion and subsequent development of listeria monocytogenes on stainless‐steel surfaces
Anne-Sophie Hascoët, Carolina Ripolles-Avila, Brayan R. H. Cervantes-Huamán, and José Juan Rodríguez-Jerez
MDPI AG
Listeria monocytogenes continues to be one of the most important public health challenges for the meat sector. Many attempts have been made to establish the most efficient cleaning and disinfection protocols, but there is still the need for the sector to develop plans with different lines of action. In this regard, an interesting strategy could be based on the control of this type of foodborne pathogen through the resident microbiota naturally established on the surfaces. A potential inhibitor, Bacillus safensis, was found in a previous study that screened the interaction between the resident microbiota and L. monocytogenes in an Iberian pig processing plant. The aim of the present study was to evaluate the effect of preformed biofilms of Bacillus safensis on the adhesion and implantation of 22 strains of L. monocytogenes. Mature preformed B. safensis biofilms can inhibit adhesion and the biofilm formation of multiple L. monocytogenes strains, eliminating the pathogen by a currently unidentified mechanism. Due to the non-enterotoxigenic properties of B. safensis, its presence on certain meat industry surfaces should be favored and it could represent a new way to fight against the persistence of L. monocytogenes in accordance with other bacterial inhibitors and hygiene operations.

Effect of an enzymatic treatment on the removal of mature Listeria monocytogenes biofilms: A quantitative and qualitative study
T. Mazaheri, C. Ripolles-Avila, A.S. Hascoët, and J.J. Rodríguez-Jerez
Elsevier BV

New approach for the removal of mature biofilms formed by wild strains of Listeria monocytogenes isolated from food contact surfaces in an Iberian pig processing plant
C. Ripolles-Avila, M. Ramos-Rubio, A.S. Hascoët, M. Castillo, and J.J. Rodríguez-Jerez
Elsevier BV

Microbial ecology evaluation of an iberian pig processing plant through implementing sch sensors and the influence of the resident microbiota on Listeria monocytogenes
Anne-Sophie Hascoët, Carolina Ripolles-Avila, Alfons Eduard Guerrero-Navarro, and José Juan Rodríguez-Jerez
MDPI AG
There is a whole community of microorganisms capable of surviving the cleaning and disinfection processes in the food industry. These persistent microorganisms can enhance or inhibit biofilm formation and the proliferation of foodborne pathogens. Cleaning and disinfection protocols will never reduce the contamination load to 0; however, it is crucial to know which resident species are present and the risk they represent to pathogens, such as Listeria monocytogenes, as they can be further used as a complementary control strategy. The aim of this study was to evaluate the resident surface microbiota in an Iberian pig processing plant after carrying out the cleaning and disinfection processes. To do so, surface sensors were implemented, sampled, and evaluated by culture plate count. Further, isolated microorganisms were identified through biochemical tests. The results show that the surfaces are dominated by Bacillus spp., Pseudomonas spp., different enterobacteria, Mannheimia haemolytica, Rhizobium radiobacter, Staphylococcus spp., Aeromonas spp., lactic acid bacteria, and yeasts and molds. Moreover, their probable relationship with the presence of L. monocytogenes in three areas of the plant is also explained. Further studies of the resident microbiota and their interaction with pathogens such as L. monocytogenes are required. New control strategies that promote the most advantageous profile of microorganisms in the resident microbiota could be a possible alternative for pathogen control in the food industry. To this end, the understanding of the resident microbiota on the surfaces of the food industry and its relation with pathogen presence is crucial.

Hygienic properties exhibited by single-use wood and plastic packaging on the microbial stability for fish
C. Ripolles-Avila, A.S. Hascoët, A.G. Ríos-Castillo, and J.J. Rodríguez-Jerez
Elsevier BV

Development of a dairy fouling model to assess the efficacy of cleaning procedures using alkaline and enzymatic products
A.E. Guerrero-Navarro, A.G. Ríos-Castillo, C. Ripolles Avila, A.S. Hascoët, X. Felipe, and J.J. Rodriguez Jerez
Elsevier BV

Evaluation of the microbiological contamination of food processing environments through implementing surface sensors in an iberian pork processing plant: An approach towards the control of Listeria monocytogenes
C. Ripolles-Avila, A.S. Hascoët, J.V. Martínez-Suárez, R. Capita, and J.J. Rodríguez-Jerez
Elsevier BV

Quantification of mature Listeria monocytogenes biofilm cells formed by an in vitro model: A comparison of different methods
C. Ripolles-Avila, B.H. Cervantes-Huaman, A.S. Hascoët, J. Yuste, and J.J. Rodríguez-Jerez
Elsevier BV

Bactericidal efficacy of UV activated TiO<inf>2</inf> nanoparticles against Gram-positive and Gram-negative bacteria on suspension
Carolina Ripolles-Avila, Maria Martinez-Garcia, Anne-Sophie Hascoët, and José Juan Rodríguez-Jerez
Informa UK Limited
ABSTRACT Two different TiO2 nanoparticles, NM101 and NM105, were evaluated against a range of Gram-positive (Staphylococcus aureus, Bacillus cereus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus acidophilus and Lactobacillus lactis) and Gram-negative (Salmonella enterica var. Enteridis and Escherichia coli) bacteria. Both NM101 and NM105 TiO2 nanoparticles (UV-exposed or none) had a significant antibacterial activity when the concentration of TiO2 suspension was 100 µg mL−1. The activation of the TiO2 NPs led, in all cases, to a shift in the growth curve, revealing lower counts as the concentration increased. E. coli was the most significantly affected pathogen by both TiO2 nanoparticles reaching among 2–3 log CFU.mL−1 reduction. In addition, in the case of the probiotic bacteria, NM105 TiO2 nanoparticles had similar effects as the bacterial density was reduced by 2–3 log CFU.mL−1. These results may be applied as a potent technology to be included in the formulation of new disinfectants.

Establishment of incubation conditions to optimize the in vitro formation of mature Listeria monocytogenes biofilms on food-contact surfaces
C. Ripolles-Avila, A.S. Hascoët, A.E. Guerrero-Navarro, and J.J. Rodríguez-Jerez
Elsevier BV

RECENT SCHOLAR PUBLICATIONS

Effectiveness of enzymatic treatment for reducing dairy fouling at pilot-plant scale under real cleaning conditions
AE Guerrero-Navarro, AG Ros-Castillo, C Ripolles-Avila, A Zamora, ...
Lwt 154, 112634 2022

In Vitro Preformed Biofilms of Bacillus safensis Inhibit the Adhesion and Subsequent Development of Listeria monocytogenes on Stainless-Steel Surfaces
AS Hascot, C Ripolles-Avila, BRH Cervantes-Huamn, ...
Biomolecules 11 (3), 475 2021

Effect of an enzymatic treatment on the removal of mature Listeria monocytogenes biofilms: A quantitative and qualitative study
T Mazaheri, C Ripolles-Avila, AS Hascot, JJ Rodrguez-Jerez
Food Control 114, 107266 2020

New approach for the removal of mature biofilms formed by wild strains of Listeria monocytogenes isolated from food contact surfaces in an Iberian pig processing plant
C Ripolles-Avila, M Ramos-Rubio, AS Hascot, M Castillo, ...
International Journal of Food Microbiology 323, 108595 2020

Microbial ecology evaluation of an iberian pig processing plant through implementing SCH sensors and the influence of the resident microbiota on Listeria monocytogenes
AS Hascot, C Ripolles-Avila, AE Guerrero-Navarro, JJ Rodrguez-Jerez
Applied Sciences 9 (21), 4611 2019

Hygienic properties exhibited by single-use wood and plastic packaging on the microbial stability for fish
C Ripolles-Avila, AS Hascot, AG Ros-Castillo, JJ Rodrguez-Jerez
LWT 113, 108309 2019

Development of a dairy fouling model to assess the efficacy of cleaning procedures using alkaline and enzymatic products
AE Guerrero-Navarro, AG Ros-Castillo, CR Avila, AS Hascot, X Felipe, ...
LWT 106, 44-49 2019

Evaluation of the microbiological contamination of food processing environments through implementing surface sensors in an iberian pork processing plant: An approach towards
C Ripolles-Avila, AS Hascot, JV Martnez-Surez, R Capita, ...
Food Control 99, 40-47 2019

Quantification of mature Listeria monocytogenes biofilm cells formed by an in vitro model: A comparison of different methods
C Ripolles-Avila, BH Cervantes-Huaman, AS Hascot, J Yuste, ...
International journal of food microbiology 289, 209-214 2019

Desarrollo de biofilms de listeria monocytogenes y microbiota acompaante en instalaciones crnicas: cuantificacin, accin sinrgica y antagonista
AS Hascot
2019

Bactericidal efficacy of UV activated TiO₂ nanoparticles against Gram-positive and Gram-negative bacteria on suspension
C Ripolles-Avila, M Martinez-Garcia, AS Hascot, JJ Rodrguez-Jerez
CyTA-Journal of Food 17 (1), 408-418 2019

Establishment of incubation conditions to optimize the in vitro formation of mature Listeria monocytogenes biofilms on food-contact surfaces
C Ripolles-Avila, AS Hascot, AE Guerrero-Navarro, JJ Rodrguez-Jerez
Food Control 92, 240-248 2018

Growth inhibition of Listeria monocytogenes by a cell-free extract of cultured bacteria isolated from a meat processing plant
AS Hascot, C Ripolles, A Guerrero, M Martnez, JJ Rodrguez-Jerez
Annals of Nutrition and Metabolism 73 (2), 79-79 2018

Listeria monocytogenes and Salmonella Typhimurium biofilm detachment from stainless steel surfaces through enzymatic technology
C Ripolles-Avila, AE Guerrero-Navarro, AS Hascoet, JJ Rodriguez-Jerez
Annals of Nutrition and Metabolism 73 (2), 80-80 2018

DEMONSTRATION OF A NEW ENZYMATIC CLEANING PROCEDURE TO REMOVE MILK FOULING IN A DAIRY PILOT PLANT
AE Guerrero-Navarro, AG Rios-Castillo, C Ripolles-Avila, AS Hascot, ...
Annals of Nutrition and Metabolism 73 (2), 84-84 2018

Reduction of animal excrement's potential as a polluant by using zeolite in feeding
AS Hascot
2016

MOST CITED SCHOLAR PUBLICATIONS

Evaluation of the microbiological contamination of food processing environments through implementing surface sensors in an iberian pork processing plant: An approach towards
C Ripolles-Avila, AS Hascot, JV Martnez-Surez, R Capita, ...
Food Control 99, 40-47 2019
Citations: 49

Hygienic properties exhibited by single-use wood and plastic packaging on the microbial stability for fish
C Ripolles-Avila, AS Hascot, AG Ros-Castillo, JJ Rodrguez-Jerez
LWT 113, 108309 2019
Citations: 14

Desarrollo de biofilms de listeria monocytogenes y microbiota acompaante en instalaciones crnicas: cuantificacin, accin sinrgica y antagonista
AS Hascot
2019
Citations: 2