Dr. Ashish warghane
@gtu.ac.in
Assistant Professor School of Applied Sciences and Technology
Gujarat Technological University
Dr. Ashish Warghane, completed his Bachelor of Science, Master of Science in Biotechnology from Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur and Ph.D in Biotechnology from SHUATS Prayagraj. He has ten years teaching and research experience in the field of Plant Virology & Molecular Diagnostics. He is principally working on the development of novel molecular diagnostic tools and control of plant viruses and virus like pathogens. He published more than 20 research articles in Scopus indexed, peer reviewed international and national journals, like Phytopathology (American Phytopathological Society), PlosOne, Journal of Virological Methods (Elsevier), Phytopathology, Plant Disease (American Phytopathological Society), Phytoparasitica (Springer), Biochimica et Biophysica Acta (Elsevier), Australasian plant pathology journal, Journal of Plant Biochemistry and Biotechnology (Springer). He also published Book chapter in Springer, Elsevier & Bentham. He is an editor of Agrifood Magazi
EDUCATION
PhD Biotechnology
M.Sc. Biotechnology
RESEARCH INTERESTS
Plant Virology, Plant Pathology, Molecular Diagnostics, Biotechnology, Microbiology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Ashish Warghane, Neha G. Paserkar, and Sumit Bhose
BENTHAM SCIENCE PUBLISHERS
Meeting the crucial demand for sustainable agriculture is an upcoming challenge worldwide, leading to global food security concerns. Approximately 50% of agricultural loss is caused by both biotic and abiotic stresses. As per the estimation of Agrios, 42% of crop loss is characterized by biotic stress alone. Bacteria are the second largest contributor in terms of economic losses caused by various plant diseases. Hence, there is a need to develop elite cultivars in amalgamation with readily available sequenced plant database and progressive genome editing. This has proved to be a groundbreaking/milestone in the field of plant breeding for any desired trait. Until now, many new plant breeding techniques (NPBTs) have been introduced for crop improvement. These techniques include site-specific mutagenesis, cisgenesis, intragenesis, breeding with transgenic inducer lines, etc. This book chapter provides a comparative understanding of enrichment in plant genome editing approach about bacterial pathogens aiming for sustainable agriculture development. This chapter also brings a broad aspect of the application, advantages, unsighted aspects of genome editing, and future challenges.
Ashish Warghane, Rashmi Saini, Manju Shri, Isha Andankar, Dilip Kumar Ghosh, and Balu Ananda Chopade
Elsevier BV
Rashmi Saini, Mohammad I. Ali, Maya Pant, and Ashish Warghane
Bentham Science Publishers Ltd.
Abstract: Natural substances have been the principal source of medications since antiquity. Natural goods are gaining popularity as a source of novel medications. This article investigates a variety of variables like plant, marine, and microbial sources that contribute to the growing interest in natural goods as a source of novel medications. Viruses have remained resistant to treatment and prevention for a longer period than other forms of life. Viral diseases can currently only be treated with a limited number of drugs. Significant research initiatives have been committed to identifying novel antiviral natural compounds to fight viruses that harm people, plants, insects, animals, fungi, and microbes. A recent study of the prevalence and sources of antiviral medications licensed by the Food and Drug Administration (FDA) has focused on natural products. Out of the estimated 250,000 higher plant species, only 5 to 15 percent have been thoroughly evaluated for the existence of bioactive substances in them, and the ability of the other species has hardly been investigated. This review aims to offer an overview of the crucial role played by natural products in the discovery and development of novel antiviral drugs with potent antiviral activity, including phytochemicals such as carbohydrates, coumarins, flavonoids, chromones, alkaloids, lignans, phenols, tannins, proteins, peptides, antiviral plant extracts, other marine, and microbial sources.
Mustafa Vohra, Manjula Babariya, Jitendrakumar S. Parmar, Narayan Kamath, Ashish Warghane, and Dolatsinh Zala
Springer Science and Business Media LLC
Ashish Warghane, Rashmi Saini, Neena K. Dhiman, Khushdil Khan, Meena Koche, Aditya Sharma, R.M. Gade, Prakash Halami, and Anunava Das
Elsevier
Anand Varma, Ashish Warghane, Neena K. Dhiman, Neha Paserkar, Vijay Upadhye, Anupama Modi, and Rashmi Saini
Frontiers Media SA
The use of nanomaterials in several fields of science has undergone a revolution in the last few decades. It has been reported by the National Institutes of Health (NIH) that 65% and 80% of infections are accountable for at least 65% of human bacterial infections. One of their important applications in healthcare is the use of nanoparticles (NPs) to eradicate free-floating bacteria and those that form biofilms. A nanocomposite (NC) is a multiphase stable fabric with one or three dimensions that are much smaller than 100 nm, or systems with nanoscale repeat distances between the unique phases that make up the material. Using NC materials to get rid of germs is a more sophisticated and effective technique to destroy bacterial biofilms. These biofilms are refractory to standard antibiotics, mainly to chronic infections and non-healing wounds. Materials like graphene and chitosan can be utilized to make several forms of NCs, in addition to different metal oxides. The ability of NCs to address the issue of bacterial resistance is its main advantage over antibiotics. This review highlights the synthesis, characterization, and mechanism through which NCs disrupt Gram-positive and Gram-negative bacterial biofilms, and their relative benefits and drawbacks. There is an urgent need to develop materials like NCs with a larger spectrum of action due to the rising prevalence of human bacterial diseases that are multidrug-resistant and form biofilms.
Sachin Misal, Ashish Warghane, and Girish Patil
Springer Science and Business Media LLC
Sunil B. Kokane, Pragati Misra, Amol D. Kokane, Mrugendra G. Gubyad, Ashish J. Warghane, Datta Surwase, M. Krishna Reddy, and Dilip Kumar Ghosh
Springer Science and Business Media LLC
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, and Dilip Kumar Ghosh
Scientific Societies
Indian citrus ringspot virus (ICRSV) is a devastating pathogen that has a particularly deleterious effect on the ‘Kinnow mandarin’, a commercial citrus crop cultivated in the northwest of India. ICRSV belongs to the Mandarivirus genus within the family of Alphaflexiviridae and has a positive sense single-stranded RNA (ssRNA) genome consisting of six open reading frames (ORFs). Severe cases of ICRSV result in a significant reduction in both the yield and quality of crops. Consequently, there is an urgent need to develop methods to detect ICRSV in an accurate and timely manner. Current methods involve a two-step reverse transcription polymerase chain reaction (RT-PCR) that is time consuming. Here, we describe a novel, one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the sensitive and rapid detection of ICRSV. To standardize the RT-LAMP assay, four different primers were designed and tested to target the coat protein gene of ICRSV. Amplification results were visualized by a color change after addition of SYBR Green I. The standardized RT-LAMP assay was highly specific and successfully detected all 35 ICRSV isolates tested from the Punjab and Haryana states of India. Furthermore, there was no cross-reaction with 17 isolates of five other citrus pathogens that are common in India. The ICRSV RT-LAMP assay developed in the present study is a simple, rapid, sensitive, specific technique. Moreover, the assay consists of only a single step and is more cost effective than existing methods. This is the first application of RT-LAMP for the detection of ICRSV. Our RT-LAMP assay is a powerful tool for the detection of ICRSV and will be particularly useful for large-scale indexing of field samples in diagnostic laboratories, in nurseries, and for quarantine applications.
ashish warghane
Kare Publishing
Objectives: The Coronavirus Disease 2019 (COVID-19) caused by SARS-CoV-2 has been the current global pandemic concern. With a high transmission rate, especially through direct contact, this disease spreads from person to person, and this has in turn led to a huge number of infections on a global scale. Methods: In present study, comparative genomic analysis was performed using 151 gene sequences of the viral spike protein retrieved from NCBI and along with its translated nucleotide sequences using MEGAX software. Variation in the nucleotide and amino acid positions were identified. Results: Our analysis revealed that 22 nucleotide variations observed in positions 13, 141, 162, 233, 284, 328, 455, 459, 716, 773, 784, 882, 1686, 1715, 1749, 1841, 2031, 2076, 2383, 2520, 2533, 3300 and 17 amino acid variations observed in position 5, 54, 78, 90, 95, 152, 153, 239, 258, 262, 572, 583, 614, 684, 677, 795 and 845. Further, phylogenetic analysis was used to uncover the patterns of spread of the virus across the affected countries. Although, certain strains showed patterns of transmission within communities, a vast majority revealed an evident mosaic pattern. Conclusion: The data obtained provides a clear understanding of variations in the nucleotide and translated nucleotide sequences, which can be targeted towards drug designing and to study evolutionary analysis.
Sunil B. Kokane, Amol D. Kokane, Pragati Misra, Ashish J. Warghane, Pranav Kumar, Mrugendra G. Gubyad, Ashwani Kumar Sharma, Kajal Kumar Biswas, and Dilip Kumar Ghosh
Elsevier BV
Ashish Warghane, Amol Kokane, Sunil Kokane, Manali Motghare, Datta Surwase, Supratik Pal Chodhury, Kajal Kumar Biswas, and Dilip Kumar Ghosh
Springer Science and Business Media LLC
D. K. Ghosh, M. Motghare, A. Kokane, S. Kokane, A. Warghane, S. Bhose, D. Surwase, and M. S. Ladaniya
Springer Science and Business Media LLC
Dilip Kumar Ghosh, Ashish Warghane, and Kajal Kumar Biswas
Springer New York
Dilip Kumar Ghosh, Sunil B. Kokane, Amol D. Kokane, Ashish J. Warghane, Manali R. Motghare, Sumit Bhose, Ashwani Kumar Sharma, and M. Krishna Reddy
Public Library of Science (PLoS)
Huanglongbing (HLB) or citrus greening is highly destructive disease that is affecting the citrus industry worldwide and it has killed millions of citrus plants globally. HLB is caused by the phloem limited, Gram negative, non-culturable, alpha-proteobacterium, ‘Candidatus Liberibacter asiaticus’. Currently, polymerase chain reaction (PCR) and real time PCR have been the gold standard techniques used for detection of ‘Ca. L. asiaticus’. These diagnostic methods are expensive, require well equipped laboratories, not user-friendly and not suitable for on-site detection of the pathogen. In this study, a sensitive, reliable, quick and low cost recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) technique has been developed as a diagnostic tool for detection of ‘Ca. L. asiaticus’. The assay was standardized by designing the specific primer pair and probe based on the conserved 16S rRNA gene of ‘Ca. L. asiaticus’. The assay was optimized for temperature and reaction time by using purified DNA and crude plant extracts and the best HLB-RPA-LFA was achieved at the isothermal temperature of 38°C for 20 to 30 min. The efficacy and sensitivity of the assay was carried out by using field grown, HLB-infected, HLB-doubtful and healthy citrus cultivars including mandarin, sweet orange cv. mosambi, and acid lime. The HLB-RPA-LFA did not show cross-reactivity with other citrus pathogens and is simple, cost-effective, rapid, user-friendly and sensitive. Thus, the HLB-RPA-LFA method has great potential to provide an improved diagnostic tool for detection of ‘Ca. L. asiaticus’ for the farmers, nurserymen, disease surveyors, mobile plant pathology laboratories, bud-wood certification and quarantine programs.
Gunjan Saini, Nidhi Sharma, Vikram Dalal, Ashish Warghane, Dilip Kumar Ghosh, Pravindra Kumar, and Ashwani Kumar Sharma
Elsevier BV
Dilip Kumar Ghosh, Sunil Kokane, Pranav Kumar, Ali Ozcan, Ashish Warghane, Manali Motghare, Swadeshmukul Santra, and Ashwani Kumar Sharma
Public Library of Science (PLoS)
Huanglongbing (HLB, also known as citrus greening) is considered to be the most devastating disease that has significantly damaged the citrus industry globally. HLB is caused by the Candidatus Liberibacter asiaticus (CLas), the fastidious phloem-restricted gram-negative bacterium, vectored by the asian citrus psyllid. To date, there is no effective control available against CLas. To alleviate the effects of HLB on the industry and protect citrus farmers, there is an urgent need to identify or develop inhibitor molecules to suppress or eradicate CLas from infected citrus plant. In this paper, we demonstrate for the first time an in planta efficacy of two antimicrobial compounds against CLas viz. 2S albumin (a plant based protein; ~12.5 kDa), Nano-Zinc Oxide (Nano-ZnO; ~ 4.0 nm diameter) and their combinations. Aqueous formulations of these compounds were trunk-injected to HLB affected Mosambi plants (Citrus sinensis) grafted on 3-year old rough lemon (C. jambhiri) rootstock with known CLas titer maintained inside an insect-free screen house. The effective concentration of 2S albumin (330 ppm) coupled with the Nano-ZnO (330 ppm) at 1:1 ratio was used. The dynamics of CLas pathogen load of treated Mosambi plants was assessed using TaqMan-qPCR assay every 30 days after treatment (DAT) and monitored till 120 days. We observed that 2S albumin-Nano-ZnO formulation performed the best among all the treatments decreasing CLas population by 96.2%, 97.6%, 95.6%, and 97% of the initial bacterial load (per 12.5 ng of genomic DNA) at 30, 60, 90, and 120 DAT, respectively. Our studies demonstrated the potency of 2S albumin-Nano-ZnO formulation as an antimicrobial treatment for suppressing CLas in planta and could potentially be developed as a novel anti CLas therapeutics to mitigate the HLB severity affecting the citrus industry worldwide.
Manali Motghare, Arun Kumar Dhar, Amol Kokane, Ashish Warghane, Sunil Kokane, Ashwani Kumar Sharma, M. Krishna Reddy, and Dilip Kumar Ghosh
Elsevier BV
Ashish Warghane, Pragati Misra, Sumit Bhose, Kajal Kumar Biswas, Ashwani Kumar Sharma, M. Krishna Reddy, and Dilip Kumar Ghosh
Elsevier BV
D. K. Ghosh, S. Bhose, P. Sharma, A. Warghane, M. Motghare, M. S. Ladaniya, M. K. Reddy, V. Thorat, and A. Yadav
Scientific Societies
Acid lime [Citrus aurantifolia (L.) Swingle] is an important citrus cultivar that constitutes nearly 20% of total citrus production in India. During a survey conducted in April 2014 to February 2015, conspicuous symptoms of small chlorotic leaves with highly proliferated shoots and shortened internodes resembling witches’-broom disease of acid lime (WBDL) were observed in four out of 22 acid lime groves in Nagpur district of Maharashtra state, India. Seven representative field samples from symptomatic plants were graft-inoculated to healthy acid lime seedlings under greenhouse conditions and all developed characteristic symptoms 18 to 20 months after grafting. Numerous phytoplasma-like bodies of 100 to 800 nm in diameter and bounded by poorly defined membrane were observed in sieve tubes of diseased samples but not in similar sections from healthy leaves when ultrathin sections of leaf midrib were observed under a JEM 100S transmission electron microscope. PCR was used to confirm the presence of phytoplasma in infected tissues from 20 symptomatic and five asymptomatic plants. Samples were analyzed using P1/P7 primer pairs for the first round of PCR and the products were subsequently amplified with nested primer pairs R16F2n/R16R2 (Lee et al. 2004). An expected amplicon of ∼1.2 kb was obtained from 18 diseased plant tissues while it was not observed in asymptomatic plants. Four amplicons obtained from different field samples using R16F2n/R16R2 were purified, sequenced, and the resultant sequences were deposited in GenBank (KY024345, KY024346, KY024347, KY024348). All obtained sequences were 100% identical. The results of NCBI-BLAST analysis further revealed that the 16S rRNA gene sequences shared 99.9% similarity with that of the ‘Candidatus Phytoplasma cynodontis’ strains (LT558777, KF234570, AB741630). Phylogenetic analysis of obtained sequences showed that the WBDL phytopalsma formed a distinct clade with ‘Ca. Phytoplasma cynodontis’ strain BGWL-C1. In silico RFLP analysis of the aligned 16S rRNA gene sequence of the WBDL phytoplasma was performed with 17 distinct restriction enzymes using iPhyClassifier (Zhao et al. 2009) and the results were compared with each phytoplasma group and subgroup. Based on the analysis, WBDL phytoplasma 16S rRNA gene sequences shared 99.8% similarity with that of the ‘Ca. Phytoplasma cynodontis’ reference strain (AJ550984). Moreover, the virtual RFLP pattern derived from the query 16S rRNA gene F2nR2 fragment was identical (similarity coefficient 1.00) to the reference pattern of 16Sr group XIV, subgroup A (AJ550984) (Marcone et al. 2004). Taken together, these results indicate that the phytoplasma associated with WBDL in India is a ‘Ca. Phytoplasma cynodontis’-related strain and belongs to 16SrXIV-A subgroup. To the best of our knowledge, this is the first report of occurrence of 16SrXIV group phytoplasma associated with WBDL not only in India but also in the world. It will be interesting to identify potential plant reservoir and insect vectors of the pathogen in this region. The disease adversely affects fruit yield and has the potential to significantly reduce economic condition of citrus growers of the region.
Nidhi Sharma, Purushotham Selvakumar, Gunjan Saini, Ashish Warghane, Dilip Kumar Ghosh, and Ashwani Kumar Sharma
Elsevier BV
Dilip Kumar Ghosh, Sumit Bhose, Ashish Warghane, Manali Motghare, Ashwani Kumar Sharma, Arun Kumar Dhar, and Siddarame Gowda
Springer Science and Business Media LLC
Dilip Kumar Ghosh, Sumit Bhose, Manali Motghare, Ashish Warghane, Krishanu Mukherjee, Dipak Kumar Ghosh, Ashwani Kumar Sharma, Milind Shivratan Ladaniya, and Siddarame Gowda
Scientific Societies
Citrus huanglongbing (HLB, citrus greening disease) is an extremely destructive disease affecting citrus and causes severe economic loss to the crop yield worldwide. The disease is caused by a phloem-limited, noncultured, gram-negative bacteria Candidatus Liberibacter spp., the widely present and most destructive species being ‘Candidatus Liberibacter asiaticus’. Although the disease has been reported from almost all citrus growing regions of India, knowledge on the molecular variability of the pathogen ‘Ca. L. asiaticus’ populations from different geographical regions and cultivars is limited. In the present study, variability of the Indian ‘Ca. L. asiaticus’ based on the tandem repeats at the genomic locus CLIBASIA_01645 was characterized and categorized into four classes based on the tandem repeat number (TRN); Class I (TRN ≤ 5), Class II (TRN > 5 ≤ 10), Class III (TRN > 10 ≤ 15), and Class IV (TRN > 15). The study revealed that the Indian population of ‘Ca. L. asiaticus’ is more diverse than reported for Florida and Guangdong populations, which showed less diversity. While Florida and Guangdong populations were dominated by a TRN5 and TRN7 genotype, respectively, the Indian ‘Ca. L. asiaticus’ populations with TRN copy numbers 9, 10, 11, 12, and 13 were widely distributed throughout the country. Additionally, TRN2 and TRN17 genotypes were also observed among the Indian ‘Ca. L. asiaticus’ populations. The predominant ‘Ca. L. asiaticus’ genotypes from the northeastern region of India were TRN6 and TRN7 (53.12%) and surprisingly similar to neighboring South China populations. Preliminary results showed absence of preference of citrus cultivars to any specific ‘Ca. L. asiaticus’ genotype.
Dilip K. Ghosh, Sumit Bhose, Krishanu Mukherjee, Balaji Aglave, A. J. Warghane, Manali Motghare, V. K. Baranwal, and Arun K. Dhar
Springer Science and Business Media LLC
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Name of Project: Molecular detection and characterization of ‘Candidatus Liberibacter from major citrus growing region of Madhya Pradesh
Funding agency: Mandsaur University Research Council, (MURC) Mandsaur
Cost: Rs. 50000.00
Status: Completed
Name of Project: Production of organic manure using Soyabean industrial waste
Funding agency: Innovation, Incubation & Intellectual Property Rights Cell (III Cell), Mandsaur University
Cost: Rs. 28000.00
status: Completed