ASHOK BEHERA
@dituniversity.edu.in
Asst Prof/Faculty of Pharmacy
DIT University
EDUCATION
Examination /Degree Board/Council/
University/Other
Examining Body Duration of the Course & Year of Completion Division/ Class with % of Marks/
Ph.D.
(Pharmacy) Jadavpur
University (CSIR-IICB) registration:
13/06/2011 awarded on 27/06/2014 submitted 18/06/2013
NA
M.S
(Pharm) National Institute of Pharmaceutical
Education and
Research
(NIPER)-Kolkata 2yr, June 2009 CGPA-
9.46/10
(89.60%)
RESEARCH INTERESTS
chemistry to chemical biology. Specifically, my research focuses on the following areas:
Peptidomimetics and Peptide conjugates as therapeutics and drug delivery
Receptor specific targeted drug delivery
Computer aided drug design
Organic synthesis and purification: Solid phase peptide synth
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Jhuma Samanta, Ashok Behera, and Aman Chaudhary
CRC Press
Ashwani Verma, Ashok Behera, Rohitashwa Kumar, Nachiket Gudi, Ashish Joshi, and KM Monirul Islam
Elsevier BV
Shraddha Manish Gupta, Ashok Behera, Neetesh K. Jain, Avanish Tripathi, Dinesh Rishipathak, Siddharth Singh, Nafees Ahemad, Meryem Erol, and Devendra Kumar
Royal Society of Chemistry (RSC)
Leading pathological markers of Alzheimer's disease (AD) include Acetylcholinesterase (AChE), Butyrylcholinesterase (BuChE), Amyloid beta (Aβ) and reactive oxygen species (ROS).
Ashwani Verma, Ashok Behera, KM Monirul Islam, and Ashish Joshi
Medsci Publications
Background: The burden of chronic obstructive pulmonary disease to patients, their caretakers, and health system is gradually increasing and is associated with the morbidity, mortality, disability adjusted life years and higher costs. Self-management of COPD is a vital strategy for its management. This study aims to develop, implement, and evaluate an informatics platform for the home-based self-management of Chronic Obstructive Pulmonary Disease. Method: A systematic review will be conducted to synthesize and appraise the evidence on self-management informatics tool or platform for COPD. An evidence-based COPD self-management application will be developed and implemented to 35 COPD patients at identified hospitals of New Delhi using prospective non-randomized study. A mixed methods study will be conducted to evaluate the effectiveness of this informatics platform at selected hospitals of Delhi. Outcomes will be measured in terms of self-management for COPD related exacerbations, number of health care facility admissions for COPD and hospitalization days, adherence to the informatics platform, number of exacerbations requiring hospital visits, health related quality of life. Conclusion: The development and evaluation of home-based self-management of informatics platform for COPD would be an innovative strategy for the COPD patients in India.
Taha Alqahtani, Sharada L. Deore, Anjali A. Kide, Bhavana A. Shende, Ritika Sharma, Rita Dadarao Chakole, Lalita S. Nemade, Nikita Kishor Kale, Sudarshana Borah, Savita Shrikant Deokar,et al.
Elsevier BV
Shraddha Manish Gupta, Siddharth Singh, Priyanka Sanwal, Samir Bhargava, Ashok Behera, Shuchi Upadhyay, Md. Habban Akhter, and Manish Gupta
Bentham Science Publishers Ltd.
Abstract: Proteins and peptides possess considerable potential in treating solid tumors because of their unique properties. At present, there are over 100 peptide-based formulations on the market. Today, peptides and proteins are in more demand due to their selective nature and high target-binding efficiency. Targeting solid tumors with compounds of molecular weight less than 10 kDa are much more desirable because they undergo excessive penetration in view of the fact that they are small sized. The solid tumors have thick tissues and possess excessive interstitial fluid pressure, because of which high molecular compounds cannot enter. The properties of proteins and peptides induce low toxic effects and lessen the major side effects caused by chemical-based drugs. However, their delivery is quite challenging as most proteins and peptides stop functioning therapeutically when following a parenteral route of administration. This paper elaborates on the importance of new age formulations of peptides and proteins followed by their recently documented advancements that increase their stability and delay their metabolism, which helps to target solid tumors.
Alok Pratap Singh, Havagiray Chitme, Rajeev Kumar Sharma, JB Kandpal, Ashok Behera, Basel A. Abdel-Wahab, Mohammed Abdelmalek Orabi, Masood Medleri Khateeb, Mohammed Habeeb, and Marwa B. Bakir
MDPI AG
In this review, we describe and discuss the phytoconstituents present in Hedychium species and emphasize their potential as drug candidates. Though they are widely validated in vitro and in vivo models, to date, no efforts have been made to compile in a single review all the pharmacologically active phytoconstituents from Hedychium species, and their pharmacological and toxicity profile. In this study, we present a reinvestigation of the chemical constituents present in Hedychium species obtained from the essential oil and solvent extraction of the flowers, leaves and rhizomes under consideration. Key databases such as PubMed, Science Direct, Scopus, and Google Scholar amongst others were probed for a systematic search using keywords to retrieve relevant publications on this plant. An exhaustive electronic survey of the related literature on Hedychium species resulted in around 200 articles. Articles published between the years 1975–2021 were included. The studies conducted on either crude extracts, solvent fractions or isolated pure compounds from Hedychium species reported with a varied range of biological effects such as anti-inflammatory, analgesic, antidiabetic, potentially anti-asthmatic, and cytotoxic, among other related activities of the chemical constituents present in its essential oil and solvent extract deployed in this review. Traditional and herbal medication around the world that uses different parts of Hedychium species were considered for anti-inflammatory, skincare, analgesic, anti-asthmatic, anti-diabetic, antidotal uses, among others. These uses support the idea that chemical constituents obtained from solvent extraction may also exert the same action individually or in a synergistic manner. The review concluded that there is scope for computation and biological study to find out possible new targets for strengthening the potency and selectivity of the relevant compounds, and to find a commercial method for extraction of active pharmaceutical ingredients.
Angshuman Ghosh, Jishu Mandal, Soumen Kumar Dubey, Somrita Padma, Narendra Nath Ghosh, Ashok Behera, Sk Abdul Hafiz, Pradip Ruidas, Ramkrishna Midya, Dipanwita Roy,et al.
Wiley
AbstractWe showed solvent‐ and concentration‐triggered chiral tuning of the fibrous assemblies of two novel glycoconjugates Z‐P(Gly)‐Glu and Z‐F(4‐N)‐Glu made by chemical attachment of Cbz‐protected [short as Z)] non‐proteinogenic amino acids L‐phenylglycine [short as P(Gly)] and 4‐Nitro‐L‐phenylalanine [short as F(4‐N)] with D‐glucosamine [short as Glu]. Both biomimetic gelators can form self‐healing and shape‐persistent gels with a very low critical gelator concentration in water as well as in various organic solvents, indicating they are ambidextrous supergelators. Detailed spectroscopic studies suggested β‐sheet secondary structure formation during anisotropic self‐aggregation of the gelators which resulted in the formation of hierarchical left‐handed helical fibers in acetone with an interlayer spacing of 2.4 nm. After the physical characterization of the gels, serum protein interaction with the gelators was assessed, indicating they may be ideal for biomedical applications. Further, both gelators are benign, non‐immunogenic, non‐allergenic, and non‐toxic in nature, which was confirmed by performing the blood parameters and liver function tests on Wister rats. Streptomycin‐loaded hydrogels showed efficacious antibacterial activity in vitro and in vivo as well. Finally, cell attachment and biocompatibility of the hydrogels were demonstrated which opens a newer avenue for promising biomedical and therapeutic applications.
Shraddha M. Gupta, Neetesh K. Jain, Rohitash Yadav, Meryem Erol, Ismail Celik, Manish Gupta, and Ashok Behera
Bentham Science Publishers Ltd.
Background: Torpedo californica acetylcholinesterase (TcAChE) is an important drug development target for Alzheimer's disease (AD) therapeutics. The current in silico study aims to recognise indene methylene-derived compounds acting against TcAChE to gain insight into the molecular interactions. Objective: The current study focused on identifying novel inhibitors for Torpedo californica acetylcholinesterase (TcAChE) by virtual screening, molecular docking, drug-likeness, molecular simulation, and DFT profile for anti-Alzheimer's activity. Methods: Molecular docking, ADMET screening, molecular simulation, and DFT were performed for drug development having anti-Alzheimer's activity related to Torpedo californica acetylcholinesterase (TcAChE). Results: On the AutoDock Vina algorithms, ligands SD-24 [-12.6, -13.1 kcal/mol], SD-30 [-12.5, -12.6 kcal/mol], SD-42 [-11.8, -12.5kcal/mol] showed promising docking and confirmatory redocking scores compared to Donepezil [-8, -10.9 kcal/mol], followed by ADMET screening. The best three complexes were subjected to molecular dynamics simulations (MDSs) over 30 ns to understand the TcAChE dynamics and behavior in a complex with the ligand. MEP and NBO analysis was performed for the DFT/B3LYP theory and 6-311G [d,p] base set and Gaussian 09 package program. For MDSs, the root means square (RMSD) parameter remained stable for 30 ns at 0.25 nm. The ligand-AChE complex formed 2 to 4 satisfactory intermolecular H bonds, which substantiated the stability of the three compounds in the protein binding cluster as potent binders. The LUMO (owest unoccupied molecular orbital)- HOMO (highest occupied molecular orbital) energy gap of the SD24, SD30, and SD42 compounds was 4.0943, 4.2489, and 4.2489 eV, respectively, and stability was ordered as SD24>SD30=SD42. Conclusion: The outcome of in silico studies suggests that SD24, SD30, and SD42 compounds have promising drug-likeness, simulation, and DFT profiles for anti-Alzheimer's activity. However, in vitro and in vivo studies are required to confirm their biological activities.
Shraddha Manish Gupta, Ashok Behera, Neetesh K. Jain, Devendra Kumar, Avanish Tripathi, Shailesh Mani Tripathi, Somdutt Mujwar, Jeevan Patra, and Arvind Negi
MDPI AG
As acetylcholinesterase (AChE) plays a crucial role in advancing Alzheimer’s disease (AD), its inhibition is a promising approach for treating AD. Sulindac is an NSAID of the aryl alkanoic acid class, consisting of a indene moiety, which showed neuroprotective behavior in recent studies. In this study, newer Indene analogs were synthesized and evaluated for their in vitro AChE inhibition. Additionally, compared with donepezil as the standard drug, these Indene analogs were accessed for their cell line-based toxicity study on SH-SY5Y cell line. The molecule SD-30, having hydrogen bond donor (HBD) at para-position, showed maximum AChE inhibition potential (IC50 13.86 ± 0.163 µM) in the indene series. Further, the SD-30 showed maximum BuChE inhibition potential (IC50 = 48.55 ± 0.136 µM) with a selectivity ratio of 3.50 and reasonable antioxidant properties compared to ascorbic acid (using DPPH assay). SD-30 (at a dose level: of 10 µM, 20 µM) effectively inhibited AChE-induced Aβ aggregation and showed no significant toxicity up to 30 mM against SH-SY5Y cell lines.
Hasina Mamataj Begam, Rajarshee Choudhury, Ashok Behera, and Ranjan Jana
American Chemical Society (ACS)
A practical copper-catalyzed, 2-picolinamide-directed ortho C-H amination of anilines with benzoyl-protected hydroxylamines has been disclosed that proceeds smoothly without any external stoichiometric oxidant or additives. Remarkably, besides anilines, bicyclic naphthyl or heterocyclic amines furnished amination products with five- and six-membered cyclic and acyclic amines at the ortho position selectively. This electrophilic C-H amination also proceeds smoothly in water under slightly modified reaction conditions.
Indranil Banerjee, Ashok Behera, Kakali De, Sankha Chattopadhyay, Satbir Singh Sachdev, Bharat Sarkar, Santanu Ganguly, and Mridula Misra
Springer Science and Business Media LLC
Ashok Behera, Kakali De, Sankha Chattopadhayay, and Mridula Misra
Springer Science and Business Media LLC
Kakali De, Asoke Behera, Indranil Banerjee, Bharat Sarkar, Santanu Ganguly, and Mridula Misra
Springer Science and Business Media LLC
Ashok Behera, Indranil Banerjee, Kakali De, Rudra Narayan Munda, Sankha Chattopadhayay, Amalesh Samanta, Bharat Sarkar, Santanu Ganguly, and Mridula Misra
Springer Science and Business Media LLC
Kakali De, Arijit Bhowmik, Ashok Behera, Indranil Banerjee, Mrinal Kanti Ghosh, and Mridula Misra
Wiley
Radiolabeled somatostatin analogs have become powerful tools in the diagnosis and staging of neuroendocrine tumors, which express somatostatin receptors. The aim of this study was to evaluate a new somatostatin analog, 6‐hydrazinopyridine‐3‐carboxylic acid‐Ser3‐octreotate (HYNIC‐SATE) radiolabeled with 99mTc, using ethylenediamine‐N,N′‐diacetic acid and tricine as coligands, to be used as a radiopharmaceutical for the in vivo imaging of somatostatin receptor subtype 2 (SSTR2)‐positive tumor. Synthesis of the peptide was carried out on a solid phase using a standard Fmoc strategy. Peptide conjugate affinities for SSTR2 were determined by receptor binding affinity on rat brain cortex and C6 cell membranes. Internalization rate of 99mTc‐HYNIC‐SATE was studied in SSTR2‐expressing C6 cells that were used for intracranial tumor studies in rat brain. A reproducible in vivo C6 glioma model was developed in Sprague–Dawley rat and confirmed by histopathology and immunohistochemical analysis. Biodistribution and imaging properties of this new radiopeptide were also studied in C6 tumor‐bearing rats. Radiolabeling was performed at high specific activities, with a radiochemical purity of >96%. Peptide conjugate showed high affinity binding for SSTR2 (HYNIC‐SATE IC50 = 1.60 ± 0.05 n m) and specific internalization into rat C6 cells. After administration of 99mTc‐HYNIC‐SATE in C6 glioma‐bearing rats, a receptor specific uptake of radioactivity was observed in SSTR‐positive organs and in the implanted intracranial tumor and rapid excretion from nontarget tissues via kidneys.
Ashok Behera, Kakali De, Susmita Chandra, Sankha Chattopadhyay, and Mridula Misra
Springer Science and Business Media LLC
Partha Chattopadhyay, Debleena Bhattacharya, Ashok Behera, and Sandip Hota
Georg Thieme Verlag KG
Palladium-catalyzed intramolecular aryl etherification reaction using bulky binaphthylphosphane ligand is shown to be a convenient method for the synthesis of seven-membered heterocy- cles. Application of this methodology to a naturally occurring pro- teinogenic L-amino acid has led to the synthesis of optically active benzoxazepine ring systems of versatile biological importance.
RECENT SCHOLAR PUBLICATIONS
MOST CITED SCHOLAR PUBLICATIONS
Publications
1. Indene-derived hydrazides targeting Acetylcholinesterase enzyme in Alzheimer: Design, Synthesis, and Biological Evaluation. IF6.5 (Q1) Pharmaceutics 2023, 15(1), 94; . Ashok Behera
2. A comprehensive review on Pharmacologically active Phyto-constituents
from Hedychium species Manuscript ID: molecules-2255689, Journal: Molecules Authors: Alok Pratap Singh, Havagiray Chitme, Rajeev Kumar Sharma *, JB
Kandpal, Ashok Behera, Basel A. Abdel-Wahab, Masood Medleri Khateeb, Mohammed
Shafiuddin Habeeb, Mohammed Abdelmalek Orabi, Marwa Mohamed Bakir
Received: 16 February 2023.
3. Computational ADME analysis and Antioxidant potential of 5-methoxy-2-methyl-1H-indol-3-yl)-N'- (substituted benzylidene) acetohydrazide derivatives for . Journal of International academy of Physical Sciences (J. Int. Acad. Phys. Sci.) Vol. 26 No. 4(2022) , 2022. ISSN 0974 -9373. Dec15,2022. (index journal) Shraddha Manish Gupta, Neetesh K Jain, Ashok Behera. .
4. A mini review on Bioequivalence & the Bioavailability study on anticancer drugs, Article ID: BEBA-MRW-23-194, Bioequivalence & Bioavailability International Journal, February 27, 2023.
5. Digital health interventions for the self-management of COPD: Protocol for a Systematic Literature Review: Digital health interventions for the self-management of COPD" to Online Journal of Public Health Informatics[OJPH
RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)
1. BERBERIN LOADED HYBRID NANOPARTICLE COMPOSITION FOR
TREATMENT OF NEURODEGENERATION” Rhythm Khatri; Dr. Habban Akhter; Dr. Ashok Behera, Dit University, filling date 27/6/2022, application no. 202211036866, published date 8/7/2022.
2. “5-Fluorouracil-1-acetic acid (5-fua) loaded chitosan nanoparticle composition and method for preparation thereof” Ram Prabesh Patel, Jagganath Sahoo, Dr. Ashok Behera, Dit University, filling date 30/6/2022, application no. 202211037593, published date 15/7/2022.
3. “Indomethacin derivatives as multitargeting agent for alzheimer's disease management and process for” Shraddha M Gupta, Dr. Neetesh K Jain, Dr. Ashok Behera, Dr. Devendra Kumar, Dr. Dinesh Rishipathak filling date 30/6/2022, application no. 202211065309, published date 25/11/2022.
4. Develop, implement, and evaluate an informatics platform for home based self- management of Chronic Obstructive Pulmonary Disease, Ashwani Verma, Dr. Ashok Behera, Dr. KM Monirul Islam Feb2023, submitted to cell DITU.