EVALUATION OF ANTICANCER ACTIVITY OF CINNAMOMUM ZEYLANICUM AGAINST BLOOD CANCER CELL LINE Sayali Hatte, Pallavi Mandave, Ashwini Jadhav Journal of Microbiology Biotechnology and Food Sciences, 2025 Worldwide, leukemia is a major health concern. It ranks as one of the prevalent forms of cancer, particularly among children and adults. It affects individual of all age. The prevalence of leukemia varies geographically, with higher rates reported in developed countries where diagnostic capabilities and healthcare infrastructure are more advanced. The goal of present study was to calculate the antioxidant activity. The antiangiogenic activity was also performed using chick chorioallantoic membrane assay. Further, the decoction was evaluated against the leukemia cancer cell line K-562 for anti-cancer activity. The EC50 of DPPH (162.77±6.59%, p≤0.0001) and FRAP (133.65±6.00 mg) activity for Cinnamomum zeylanicum decoction was found to be higher as compared to standard (51.31±1.29% and 115.81±7.6 mg, respectively). The total phenol and flavonoid content of Cinnamomum zeylanicum decoction were 49.66±1.23 mg/GAE/100g and 12.67±0.03 mg/ RTE/ 100g, respectively. In the CAM assay, Cinnamomum zeylanicum decoction treatment shows less veins formation as compare to negative control. The Cinnamomum zeylanicum decoction treated group showed decrease in hemoglobin content than both the positive and negative controls. The decoction showed major anticancer activity against k 562 in contrast with Adriyamycin, a standard drug. The IC50 value observed for ADR was similar to cinnamon which is standard anti-cancer drug. Cinnamon was discovered to be the most potent cytotoxic agent, inhibiting K-562 cell proliferation by more than 50% at concentrations. Based on the study, we recommended the use of Cinnamomum zeylanicum as a possible remedy and preventative measure for cancer.
Exploring the diversity of vaginal microbiota between healthy women and cervical cancer patients in India Ashwini Kamble, Shilpa Naik, Manju Talathi, Deepali Jadhav, Meena Sakharkar, Jian Yang, Om Prakash, Ruchika Kaul-Ghanekar Journal of Medical Microbiology, 2024 Introduction. Cervicovaginal diversity has been reported as a predictive biomarker for cervical cancer risk. We recently reported the bio-therapeutic potential of vaginal probiotics from healthy Indian women against vaginal pathogens, isolated from the invasive cervical cancer (ICC) patients. Gap Statement. The cervicovaginal microflora from cervical cancer patients has not yet been reported from Indian population. Aim. The present study aimed at comparing the cervicovaginal microbiome between healthy controls (HC) and ICC patients from the Indian population. Methodology. In total, 30 vaginal swabs (15 from HC and 15 from ICC) were subjected to 16S rRNA gene sequencing. Alpha diversity was evaluated by Shannon and Chao1 index; and beta diversity by principle coordinate analysis (PCoA) of weighted and unweighted UniFrac distances. The relative abundance of the microbial taxa was done according to linear discriminant analysis effect size (LEfSe). Results. Predominance of Staphylococcus spp. in ICC and Lactobacillus gasseri in HC groups was observed. Alpha-diversity was found to be higher in ICC as compared to HC but was statistically non-significant. LEfSe analysis revealed Bacteroides fragilis and Escherichia coli as the marker genera in ICC with a marked decrease in Lactobacillus sp. Contrarily, in HC, L. gasseri, L. iners and L. fermentum were found to be abundant. Conclusion. Differences in the vaginal microbiome between healthy and ICC women could help in the early prediction of cervical cancer risk and thus in designing prevention strategies.
Nitrile hydratase of Rhodococcus erythropolis: Characterization of the enzyme and the use of whole cells for biotransformation of nitriles Ashwini L. Kamble, Linga Banoth, Vachan Singh Meena, Amit Singh, Yusuf Chisti, U. C. Banerjee 3 Biotech, 2013 The intracellular cobalt-type nitrile hydratase was purified from the bacterium Rhodococcuserythropolis. The pure enzyme consisted of two subunits of 29 and 30 kDa. The molecular weight of the native enzyme was estimated to be 65 kDa. At 25 °C the enzyme had a half-life of 25 h. The Michaelis–Menten constants Km and vmax for the enzyme were 0.624 mM and 5.12 μmol/min/mg, respectively, using 3-cyanopyridine as the substrate. The enzyme-containing freely-suspended bacterial cells and the cells immobilized within alginate beads were evaluated for converting the various nitriles to amides. In a packed bed reactor, alginate beads (2 % alginate; 3 mm bead diameter) containing 200 mg/mL of cells, achieved a conversion of >90 % for benzonitrile and 4-cyanopyridine in 38 h (25 °C, pH 7.0) at a feed substrate concentration of 100 mM. The beads could be reused for up to six reaction cycles.
Stereo-selective conversion of mandelonitrile to (R)-(-)-mandelic acid using immobilized cells of recombinant Escherichia coli Sandip V. Pawar, Vachan Singh Meena, Shubhangi Kaushik, Ashwini Kamble, Sandeep Kumar, Yusuf Chisti, U. C. Banerjee 3 Biotech, 2012 Immobilized cells of a recombinant Escherichia coli expressing nitrilase from Pseudomonas putida were used to catalyze the hydrolysis of mandelonitrile (2-hydroxy-2-phenylacetonitrile) to (R)-(−)-mandelic acid. The cells had been immobilized by entrapment in an alginate matrix. Conditions for the hydrolysis reaction were optimized in shake flasks and in a packed bed reactor. In shake flasks the best conditions for the reaction were a temperature of 40 °C, pH 8, biocatalyst bead diameter of 4.3 mm, sodium alginate concentration in the gel matrix of 2 % (w/v, g/100 mL), a cell dry mass concentration in the bead matrix of 20 mg/mL, an initial substrate concentration of 50 mM and a reaction time of 60 min. Under these conditions, the conversion of mandelonitrile was nearly 95 %. In the packed bed reactor, a feed flow rate of 20 mL/h at a substrate concentration of 200 mM proved to be the best at 40 °C, pH 8, using 4.3 mm beads (2 % w/v sodium alginate in the gel matrix, 20 mg dry cell concentration per mL of gel matrix). This feed flow rate corresponded to a residence time of 0.975 h in the packed bed.
Effect of agitation and aeration on the production of nitrile hydratase by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor A.L. Kamble, V.S. Meena, U.C. Banerjee Letters in Applied Microbiology, 2010 AIMS: To evaluate the effect of different physicochemical parameters such as agitation, aeration and pH on the growth and nitrile hydratase production by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor. METHODS AND RESULTS: Rhodococcus erythropolis MTCC 1526 was grown in 7-l reactor at different agitation, aeration and controlled pH. The optimum conditions for batch cultivation in the reactor were an agitation rate of 200 rev min(-1) , aeration 0.5 v/v/m at controlled pH 8. In this condition, the increase in nitrile hydratase activity was almost threefold compared to that in the shake flask. CONCLUSION: Agitation and aeration rate affected the dissolved-oxygen concentration in the reactor which in turn affected the growth and enzyme production. SIGNIFICANCE AND IMPACT OF THE STUDY: Cultivation of R. erythropolis MTCC 1526 in the reactor was found to have significant effect on the growth and nitrile hydratase production when compared to the shake flask.
