Molecular characterization of Cryptosporidium spp. In dogs and cats in the city of Rio de Janeiro, Brazil, reveals potentially zoonotic species and genotype Amanda Gleyce Lima de Oliveira, Adriana Pittella Sudré, Teresa Cristina Bergamo do Bomfim, Helena Lúcia Carneiro Santos Plos One, 2021 Intestinal cryptosporidiosis is a diarrheal disease caused by protists of genus Cryptosporidium that infect a wide variety of hosts, primarily vertebrates. Due to the close contact between humans and their companion animals, especially dogs and cats, there is concern about the potential for zoonotic transmission of this enteric protozoan parasite by infected animals. This study aimed to perform a microscopic and molecular diagnosis of Cryptosporidium spp. in fecal samples from domiciled dogs and cats. One hundred and nineteen fecal samples were processed using sugar centrifugal flotation followed by molecular detection of Cryptosporidium spp. DNA using nested PCR. Subtyping of isolates positive for C. parvum was performed by sequence analysis of the 60 kDa glycoprotein gene (GP60). Cryptosporidium oocysts were detected in 7.8% (5/64) and 5.4% (3/55) of the fecal samples from dogs and cats, respectively. Cryptosporidium canis (n = 3) and C. parvum (n = 2) were the main species found in dogs, whereas C. felis (n = 3) was prevalent in cats. Subtype IIaA17G2R2 (potentially zoonotic) was identified in samples positive for C. parvum. Despite the low prevalence of Cryptosporidium observed in the domiciled dogs and cats, the presence of potentially zoonotic C. parvum in dogs evidences a public health concern. Further research is needed to better understand the epidemiology, source, and potential impacts of Cryptosporidium infection in cats and dogs.
Occurrence of anti-toxoplasma gondii antibodies and risk factors associated with infection in equids slaughtered for human consumption in Brazil Igor Falco Arruda, Wellington Alves de Freitas, Kênia de Fátima Carrijo, Paula Silva da Paz, Marianny Miranda Silva, et al. Revista Brasileira De Parasitologia Veterinaria, 2020 Toxoplasmosis is a worldwide zoonosis caused by Toxoplasma gondii. Ingestion of raw/undercooked meat is considering an important route of infection. Consumption of meat from equids is common in European and Asian countries and an increase in Brazil has been observed. The aim of this study was to evaluate occurrences of anti-T. gondii antibodies and risk factors relating to infection in equids slaughtered for human consumption in Minas Gerais, Brazil. Blood samples from 192 horses and 208 donkeys were collected in the exsanguination area during the slaughter. Serum samples were subjected to the indirect fluorescent antibody test (IFAT). Association analysis was performed using Pearson’s chi-square test (χ2) or Fisher’s exact test, to evaluate risk factors relating to the prevalence of seroreagents. Antibodies against T. gondii were found in 13.5% of the equids, with higher occurrence in horses (18.75%) than in donkeys (8.65%). Associations between seropositivity and the following variables were found (p ≤ 0.05): species, animal origin, purpose of rearing and source of water for animal consumption and contact with cats. Farms need to implement preventive measures to control T. gondii infection in these species and avert transmission of the parasite to the human population that will consume their meat.
Are immunoenzymatic tests for intestinal protozoans reliable when used on archaeological material? Daniela Leles, Liesbeth Frías, Adauto Araújo, Beatriz Brener, Adriana Sudré, et al. Experimental Parasitology, 2019 Intestinal protozoans found in ancient human samples have been studied primarily by microscopy and immunodiagnostic assays. However, such methods are not suitable for the detection of zoonotic genotypes. The objectives of the present study were to utilize immunoenzimatic assays for coproantigen detection of Cryptosporidium sp., Giardia duodenalis, and Entamoeba histolytica/Entamoeba dispar in sixty ancient human and animal samples collected from 14 archaeological sites in South America, and to carry out a critical analysis of G. duodenalis according to results obtained from three diagnostic methodologies: microscopy, immunodiagnostic tests (immunoenzymatic and immunofluorescence), and molecular biology (PCR and sequencing). More than half (31/60) of the samples analyzed using immunoenzymatic tests were positive for at least one of the intestinal protozoans, with 46.6% (28/60) corresponding to G. duodenalis, 26.6% (16/60) to Cryptosporidium sp., and 5% (3/60) to E. histolytica/E. dispar. Cryptosporidium sp. and G. duodenalis coinfection was observed in 15% (9/60) of the samples, whereas all three protozoans were found in 5% (3/60) of samples. In the Northeast Region of Brazil, by immunoenzymatic tests there is evidence that G. duodenlais and Cryptosporidium sp. have infected humans and rodents for at least 7150 years. However, for G. duodenalis, the results from the three diagnostic tests were discordant. Specifically, despite the efficiency of the molecular biology assay in the experimental models, G. duodenalis DNA could not be amplified from the ancient samples. These results raise the following question: Are all ancient samples positive for coproantigen of G. duodenalis by immunoenzymatic tests truly positive? This scenario highlights the importance of further studies to evaluate the sensitivity and specificity of the immunoenzymatic method in the archaeological context.
PREVALENCE AND MOLECULAR CHARACTERIZATION OF DIROFILARIA IMMITIS (FILARIOIDEA: ONCHOCERCIDAE) IN DOGS FROM ENDEMIC AREAS OF RIO DE JANEIRO STATE, BRAZIL M. S. G. Silva, D. Leles, A. P. Sudré, P. R. Millar, F. Uchôa, et al. Journal of Parasitology, 2019 Canine dirofilariasis is common in Brazil, but molecular diagnosis is rare even though molecular studies increase our knowledge about molecular epidemiology and circulating genotypes from helminths worldwide. This study aims to estimate the prevalence of infection with a modified Knott's test and to perform molecular characterization of Dirofilaria immitis (Leidy, 1856) Railliet and Henry, 1911, in dogs from endemic areas of Maricá and Niterói municipalities, Rio de Janeiro State, Brazil. Molecular characterization was performed in 33 blood samples from dogs positive for microfilariae and 4 adult worms obtained from 2 other dogs. DNA extraction followed by PCR for mitochondrial target 12S rDNA and cytochrome oxidase subunit 1 (COI) of D. immitis were performed, and the amplified products were sequenced. All sequences were identical for both gene targets and showed 100% identity with D. immitis sequences from different animal species from various countries. The study concluded that this genotype of D. immitis might be dispersed worldwide.
INCREASING THE EFFICIENCY OF SPONTANEOUS SEDIMENTATION FOR THE DIAGNOSIS OF INTESTINAL PROTOZOA Clarissa Nascimento da Silveira Raso, P. Millar, B. Brener, Danuza Pinheiro Bastos Garcia de Mattos, D. Leles, et al. Journal of Parasitology, 2019 The aim of this study was to evaluate the association between a longer sedimentation time, the reading of a larger number of slides, and the collection of multiple samples on the efficiency of the spontaneous sedimentation technique. Twenty-two patients with a previous parasitological exam positive for intestinal protozoa were recruited to collect new fecal samples (3 samples per patient) before the beginning of antiparasitic treatment. All collected fecal samples were used for spontaneous sedimentation and centrifuge-flotation techniques. Of these, all 22 patients were positive based on spontaneous sedimentation, and 59.1% (13/22) based on centrifuge flotation. The number of samples and the number of slides analyzed by spontaneous sedimentation influenced the number of positive cases. The modifications applied to the spontaneous sedimentation technique increased its performance in protozoa diagnosis.
Accidental and late parasitological diagnosis of Leishmania sp. In a dog from a low disease transmission area of Brazil: A case report Renan M. Spinelli, Flávia F.M. Uchôa, Rodrigo C. Menezes, Fernanda N. Santos, Adriana P. Sudré Austral Journal of Veterinary Sciences, 2019 Canine Leishmaniasis diagnosis must be fast and accurate since dogs are urban reservoirs of the disease and earlier therapeutic intervention is more clinically effective. However, this still represents a challenge, particularly in low transmission areas. The present report describes the difficulties of clinical suspicion and the late diagnosis of a dog infected with Leishmania sp.
Frequency of enteroparasitoses in preschool children attending daycare centers: A survey applying parasitological and immunological methods Gabriela Cardoso Goes, Karina Costa Coelho Gonçalves, Adriana Pittella Sudré, Danuza Pinheiro Bastos Garcia Mattos, Beatriz Brener, et al. Journal of Tropical Pathology, 2019 The present study evaluated the frequency of intestinal parasitoses in children in public day care centers applying parasitological and immunological diagnostic methods. Fecal samples from 121 children from six public daycare centers were analyzed using parasitological techniques. Epidemiological data were obtained through a questionnaire, where parents and / or guardians were asked, for instance, whether the children had contact with soil, ate raw food, such as vegetables or raw or undercooked meat, normally walked around barefoot or had contact with animals. Fecal samples from 82 children were also tested for Giardia intestinalis and Cryptosporidium sp. coproantigen using the enzyme-linked immunosorbent assay (ELISA) which was also used for Entamoeba coproantigen detection only in samples that tested positive for the parasite by parasitological stool exam/optical microscopy. Intestinal parasite infection was noted in 23.1% (28/121) of the children. The most frequent parasite was Giardia intestinalis (13.2%), followed by Entamoeba coli (5.8%), Blastocystis spp. (1.7%), Endolimax nana (1.7%), Enterobius vermicularis (1.7%), Cystoisospora belli (0.8%),Entamoeba histolytica/E. dispar complex (0.8%), and Ascaris lumbricoides (0.8%). Positivity for parasite infection using parasitological stool exams was significantly associated with age groups, with a higher frequency in 4 to 6 year old children (p=0.03). No association or significant variations were noted in the prevalence of intestinal parasites in relation to the epidemiological variables studied. All samples were negative for Cryptosporidium sp. and Entamoeba histolytica detected by immunological testing, and 17.1% (14/82) children tested positive for Giardia intestinalis, although using parasitological exam/optical microscopy, only 14.6% (12/82) tested positive. The high incidence of intestinal parasites, especially protozoans, suggests probable interpersonal transmission among the children, environmental contamination, or even contaminated food/water intake. Thus, consolidation of preventive measures and efficient diagnostic resources as well as control of intestinal parasites and patient treatment are of utmost importance.
Toxoplasma gondii molecular and immunological identification and risk factors associated with infection in chicken slaughtered at triângulo Mineiro region, Minas Gerais, Brazil Karina Costa Coelho Gonçalves, Maria Regina Reis Amendoeira, Kênia De Fátima Carrijo, Daniela Leles, Guilherme Mendes Borges Nunes, et al. Brazilian Journal of Veterinary Research and Animal Science, 2018 This study aimed to determine the occurrence of anti-Toxoplasma gondii antibodies in the serum of slaughtered chickens in the region of Triângulo Mineiro, Minas Gerais, Brazil, to detect the parasite in tissues (heart and brain) of serologically positive chickens, based on molecular analysis, and to investigate risk variables associated with the infection. Sera from 417 chickens raised in extensive, semi-intensive, and intensive production systems were tested by an indirect immunofluorescent antibody test (IFAT) and indirect hemagglutination antibody test (IHA). Polymerase chain reaction (PCR) was performed to detect T. gondii DNA in brain and heart tissues. Antibody anti-T. gondii were found in 37.65% (157/417) of chickens by IFAT, and in 75.06% (313/417) by IHA. The Kappa index showed a weak concordance between the techniques (0.087). Association was observed between seropositivity and the variables, age (p < 0.0001), type of feeding (p < 0.0001) and collective raising with other animal’s species (p < 0.0001). Association, based on IFAT, was not observed between seropositivity and the variables, sex (p = 0.0526), presence of cats (p > 0.9999), and presence of rats (p > 0.9999). Presence of parasite DNA was detected in brain samples from two chickens, which were raised in intensive and semi-intensive production systems. The results suggest the meat of these slaughtered animals may serve as a transmission source of this protozoan to humans.
Educação em Tempos de Crise: Preparo e Execução do Ensino Remoto Emergencial na Universidade Federal Fluminense sob a Ótica de Coordenadores de Curso JR Gonçalves, TCL Bargut, S Pereira, C Fernandes-Santos, A Sudré EaD em Foco 15 (1), e2429-e2429 , 2025 2025
Rediscovering forgotten collections and their impact on society for the preservation of threatened archaeological sites D Leles, P Millar, D Mattos, SMDE Souza, C Rodrigues-Carvalho, ... Anais da Academia Brasileira de Ciências 97 (1), e20241328 , 2025 2025 Citations: 1
Association of toll-like receptors in malaria susceptibility and immunopathogenesis: A meta-analysis ADR Ramirez, MCS de Jesus, J Rossit, NF Reis, MC Santos-Filho, ... Heliyon 8 (4) , 2022 2022 Citations: 17
Identificação molecular de Cryptosporidium baileyi em patos Muscovy (Cairina moschata domesticus) em sistemas de produção ao ar livre LTD Berriel, AP Sudré, HLC Santos, TCB Bomfim Ciência Rural 52, e20210001 , 2022 2022
Molecular identification of Cryptosporidium baileyi in Muscovy ducks ( Cairina moschata domesticus ) in free-range production systems LTD Berriel, AP Sudré, HLC Santos, TCB Bomfim Ciência Rural 52 (5), e20210001 , 2021 2021 Citations: 3
Molecular characterization of Cryptosporidium spp. in dogs and cats in the city of Rio de Janeiro, Brazil, reveals potentially zoonotic species and genotype AGL de Oliveira, AP Sudré, TC Bergamo do Bomfim, HLC Santos PLoS One 16 (8), e0255087 , 2021 2021 Citations: 18
Desenvolvimento docente para o ensino remoto: experiência do Programa de Inovação e Assessoria Curricular (PROIAC) da Universidade Federal Fluminense DM Ferreira, AP Sudré, CRL Rabello, C Fernandes-Santos, S Pereira, ... EaD em Foco 11 (2) , 2021 2021 Citations: 8
PREVALÊNCIA E FATORES DE RISCO ASSOCIADOS À INFECÇÃO POR ENTEROPARASITOSES NAS CRECHES E ESCOLAS DO BRASIL: UMA REVISÃO BIBLIOGRÁFICA JVB Costa, JM da Silva Filho, AP Sudré Revista Multidisciplinar em Saúde 2 (1), 21-21 , 2021 2021
Occurrence of anti- Toxoplasma gondii antibodies and risk factors associated with infection in equids slaughtered for human consumption in Brazil IF Arruda, WA Freitas, KF Carrijo, PS Paz, MM Silva, AP Sudré, ... Revista Brasileira de Parasitologia Veterinária 29 (3), e002320 , 2020 2020 Citations: 9
Ocorrência de anticorpos anti- Toxoplasma gondii e fatores de risco associados a infecção em equídeos abatidos para consumo humano no Brasil IF Arruda, WA Freitas, KF Carrijo, PS Paz, MM Silva, AP Sudré, ... Revista Brasileira de Parasitologia Veterinária 29, e002320 , 2020 2020
Giardia duodenalis associated with other gastrointestinal parasites in sheep in the North of the Brazilian state of Parana BB De Figueiredo 2020
Are immunoenzymatic tests for intestinal protozoans reliable when used on archaeological material? D Leles, L Frías, A Araújo, B Brener, A Sudré, M Chame, V Laurentino Experimental Parasitology 205, 107739 , 2019 2019 Citations: 8
Frequency of enteroparasitoses in preschool children attending daycare centers: a survey applying parasitological and immunological methods GC Góes, KCC Gonçalves, AP Sudré, DPBG Mattos, B Brener, PB Cruz, ... Revista de Patologia Tropical/Journal of Tropical Pathology 48 (2), 121-133 , 2019 2019 Citations: 3
Increasing the efficiency of spontaneous sedimentation for the diagnosis of intestinal Protozoa CN da Silveira Raso, PR Millar, B Brener, DPBG de Mattos, D Leles, ... The Journal of Parasitology 105 (2), 248-251 , 2019 2019 Citations: 2
Prevalence and molecular characterization of Dirofilaria immitis (Filarioidea: Onchocercidae) in dogs from endemic areas of Rio de Janeiro state, Brazil MSG Silva, D Leles, AP Sudré, PR Millar, F Uchôa, B Brener The Journal of parasitology 105 (2), 387-390 , 2019 2019 Citations: 7
Identificação molecular e imunológica de Toxoplasma gondii e fatores de risco associados com infecção em galinhas abatidas na região do Triângulo Mineiro, Minas Gerais, Brasil. KCC Gonçalves, MRR Amendoeira, KF Carrijo, D Leles, GMB Nunes, ... 2019
Accidental and late parasitological diagnosis of Leishmania sp. in a dog from a low disease transmission area of Brazil: a case report RM Spinelli, FFM Uchôa, RC Menezes, FN Santos, AP Sudré Austral journal of veterinary sciences 51 (3), 131-134 , 2019 2019 Citations: 1
Methodological innovations for the study of irreplaceable samples reveal giardiasis in extinct animals (Nothrotherium maquinense and Palaeolama maior) D Leles, P Cascardo, E Pucu, B Brener, A Sudré, E Alves, F Uchoa, ... Parasitology International 67 (6), 776-780 , 2018 2018 Citations: 10
Assessment of the diagnostic performance of four methods for the detection of Giardia duodenalis in fecal samples from human, canine and feline carriers. FFM Uchcircumflex˜ a, AP Sudré, SDE Campos, NRP Almosny 2018
Captive-bred neotropical birds diagnosed with Cryptosporidium Avian genotype III RS Novaes, MS Pires, AP Sudré, TCB do Bomfim Acta Tropica 178, 297-302 , 2018 2018 Citations: 11
MOST CITED SCHOLAR PUBLICATIONS
Assessment of the diagnostic performance of four methods for the detection of Giardia duodenalis in fecal samples from human, canine and feline carriers FF de Mendonça Uchôa, AP Sudré, SDE Campos, NRP Almosny Journal of microbiological methods 145, 73-78 , 2018 2018.0 Citations: 54
Diagnóstico da estrongiloidíase humana: importância e técnicas AP Sudré, HW Macedo, RHS Peralta, JM Peralta Revista de Patologia Tropical/Journal of Tropical Pathology 35 (3), 173-184 , 2006 2006.0 Citations: 46
The influence of serial fecal sampling on the diagnosis of giardiasis in humans, dogs, and cats FFM Uchôa, AP Sudré, DB Macieira, NRP Almosny Revista do Instituto de Medicina Tropical de São Paulo 59, e61 , 2017 2017.0 Citations: 45
Toxoplasmosis-related knowledge among pregnant and postpartum women attended in public health units in Niterói, Rio de Janeiro, Brazil PR Millar, FL Moura, OMP Bastos, DPBG Mattos, ABM Fonseca, AP Sudré, ... Revista do Instituto de Medicina Tropical de São Paulo 56, 433-438 , 2014 2014.0 Citations: 45
Identification of a 26-kDa protein fraction as an important antigen for application in the immunodiagnosis of strongyloidiasis AP Sudré, RC Siqueira, MGM Barreto, RHS Peralta, HW Macedo, ... Parasitology research 101 (4), 1117-1123 , 2007 2007.0 Citations: 41
Frequência de enteroparasitas em amostras fecais de cães e gatos dos municípios do Rio de Janeiro e Niterói B Brener, L Lisboa, D Mattos, EKN Arashiro, PR Millar, AP Sudré, ... Revista Brasileira de Ciência Veterinária 12 (1), 102-105 , 2005 2005.0 Citations: 28
Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium . MCM Couto, AP Sudre, MF Lima, TCB Bomfim 2013.0 Citations: 20
Molecular characterization of Cryptosporidium spp. in dogs and cats in the city of Rio de Janeiro, Brazil, reveals potentially zoonotic species and genotype AGL de Oliveira, AP Sudré, TC Bergamo do Bomfim, HLC Santos PLoS One 16 (8), e0255087 , 2021 2021.0 Citations: 18
Estudo da contaminação de praças públicas de três municípios do estado do Rio de Janeiro, Brasil, por ovos e larvas de helmintos B maria cardoso Brener, DPBG de Mattos, PR Millar, EKN Arashiro, ... Revista de Patologia Tropical/Journal of Tropical Pathology 37 (3), 255-267 , 2008 2008.0 Citations: 18
Association of toll-like receptors in malaria susceptibility and immunopathogenesis: A meta-analysis ADR Ramirez, MCS de Jesus, J Rossit, NF Reis, MC Santos-Filho, ... Heliyon 8 (4) , 2022 2022.0 Citations: 17
First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil. AP Sudre, D Leles, MF Lima, TCB Bomfim Veterinární medicína 59 (6) , 2014 2014.0 Citations: 14
Lagochilascariasis in a housecat and the potential risk for human disease AP Sudré, F Uchôa, B Brener Brazilian Journal of Infectious Diseases 16 (1), 111-112 , 2012 2012.0 Citations: 14
Estudo da contaminação de moedas e cédulas de dinheiro circulantes na cidade de Niterói-RJ AP Sudré, BO de Pereira Franco, B Zaniboni, D de Souza Gonçalves, ... Revista de Patologia Tropical/Journal of Tropical Pathology 41 (4) , 2012 2012.0 Citations: 12
Captive-bred neotropical birds diagnosed with Cryptosporidium Avian genotype III RS Novaes, MS Pires, AP Sudré, TCB do Bomfim Acta Tropica 178, 297-302 , 2018 2018.0 Citations: 11
Conhecimento sobre toxoplasmose entre gestantes e puérperas atendidas na rede pública de saúde do município de Niterói, Rio de Janeiro, Brasil PR Millar, FL Moura, OMP Bastos, DPBG Mattos, ABM Fonseca, AP Sudré, ... Revista Do Instituto de Medicina Tropical de Sao Paulo 56, 433-438 , 2014 2014.0 Citations: 11
Bergamo do Bomfim, TC, and Santos, HLC (2021). Molecular characterization of Cryptosporidium spp. in dogs and cats in the city of Rio de Janeiro, Brazil, reveals potentially … AGL De Oliveira, AP Sudré Plos one 16 (8), e0255087 , 0 Citations: 11
Methodological innovations for the study of irreplaceable samples reveal giardiasis in extinct animals (Nothrotherium maquinense and Palaeolama maior) D Leles, P Cascardo, E Pucu, B Brener, A Sudré, E Alves, F Uchoa, ... Parasitology International 67 (6), 776-780 , 2018 2018.0 Citations: 10
Occurrence of anti- Toxoplasma gondii antibodies and risk factors associated with infection in equids slaughtered for human consumption in Brazil IF Arruda, WA Freitas, KF Carrijo, PS Paz, MM Silva, AP Sudré, ... Revista Brasileira de Parasitologia Veterinária 29 (3), e002320 , 2020 2020.0 Citations: 9
Desenvolvimento docente para o ensino remoto: experiência do Programa de Inovação e Assessoria Curricular (PROIAC) da Universidade Federal Fluminense DM Ferreira, AP Sudré, CRL Rabello, C Fernandes-Santos, S Pereira, ... EaD em Foco 11 (2) , 2021 2021.0 Citations: 8
Are immunoenzymatic tests for intestinal protozoans reliable when used on archaeological material? D Leles, L Frías, A Araújo, B Brener, A Sudré, M Chame, V Laurentino Experimental Parasitology 205, 107739 , 2019 2019.0 Citations: 8
