Anastasiia Koniaeva

Scopus Publications

EFFECTS OF BIOCOMPATIBLE PIEZOELECTRIC MEMBRANES ON THE DEVELOPMENT OF FIBROSIS ASSOCIATED WITH THE ORAL MUCOSAL WOUND REGENERATION
AD Koniaeva, EYu Varakuta, AE Leiman, GM Kormashov, MV Fedosova, EN Bolbasov, and KS Stankevich
Pirogov Russian National Research Medical University
Prevention of fibrosis during the oral mucosal wound regeneration is a pressing issue of today’s surgical dentistry. The study was aimed to perform morphological assessment of the effects of biocompatible piezoelectric membranes on fibrous tissue formation during regeneration of the oral mucosal wounds. We assessed cell–cell interactions of macrophages and fibroblasts, along with changes in the CD68 and TGFβ1 marker expression and their effects on the development of fibrosis under conditions of using biocompatible polymeric membranes with piezoelectric properties at various stages of the oral mucosal wound defect regeneration. Comparative morphological assessment of the oral mucosal structures was conducted in animals having intact mucosa (n = 15), having open wound defects (n = 15), and having wounds covered with biocompatible piezoelectric membranes (n = 15). Biomaterial was collected from the wound defect site on days 3, 7, and 12 of the experiment; collection of biomaterial from intact animals was performed on the same days. In the group, where biocompatible membranes were used, signs of proliferation phase at the defect site were detected as early as on day 3 of the study; the faster shift from macrophage infiltration to fibroblast infiltration, the decline in inflammatory response were detected on day 7; restoration of the numerical density of macrophages and fibroblasts to the intact values was detected on day 12. The expression of CD68 and TGFβ1, the prognostic markers of fibrosis, was lower in the group, where no membranes were used. Reduction of the dense fibrous connective tissue specific area was observed at the microscopic level, severe soft tissue deformation was reported at the macroscopic level. In the group with no wound covering, extensive cell infiltration and increased CD68 and TGFβ1 expression persisted throughout the experiment, which resulted in the fact that specific area of dense fibrous connective tissue was larger, than that of loose connective tissue, on day 12 of the study, as well as in the cicatricial soft tissue deformities.

Piezoelectric and Dielectric Electrospun Fluoropolymer Membranes for Oral Mucosa Regeneration: A Comparative Study
Ulyana V. Chernova, Elena Yu. Varakuta, Anastasiia D. Koniaeva, Arina E. Leyman, Sofia A. Sagdullaeva, Evgenii Plotnikov, Evgeniy Yu. Melnik, Tuan-Hoang Tran, Sven Rutkowski, Valeriya L. Kudryavtseva,et al.
American Chemical Society (ACS)
Wound healing of the oral mucosa is an urgent problem in modern dental surgical practice. This research article presents and compares the findings of the investigations of the structural, physicochemical, and biological characteristics of two types of polymeric membranes used for the regeneration of oral mucosa. The membranes were prepared from poly(tetrafluoroethylene) (PTFE) and a copolymer of vinylidene fluoride and tetrafluoroethylene (VDF-TeFE) and analyzed via scanning electron microscopy, atomic force microscopy, X-ray diffraction analysis, and Fourier transform infrared spectroscopy. Investigation results obtained indicate that both types of membranes are composed of thin fibers: (0.57 ± 0.25) μm for PTFE membranes and (0.43 ± 0.14) μm for VDF-TeFE membranes. Moreover, the fibers of VDF-TeFE membranes exhibit distinct piezoelectric properties, which are confirmed by piezoresponse force microscopy and X-ray diffraction. Both types of membranes are hydrophobic: (139.7 ± 2.5)° for PTFE membranes and (133.5 ± 2.0)° for VDF-TeFE membranes. In vitro assays verify that both membrane types did not affect the growth and division of mice fibroblasts of the 3T3-L1 cell line, with a cell viability in the range of 88-101%. Finally, in vivo comparative experiments carried out using Wistar rats demonstrate that the piezoelectric VDF-TeFE membranes have a high ability to regenerate oral mucosa.

Exploring the effect of p-tyrosol on vascular morphofunctional changes in lip mucous membrane during experimental stomatitis
A.D. Koniaeva, , N.N. Baitinger, Е.Yu. Varakuta, L.R. Mustafina, S.V. Logvinov, A.V. Gerasimov, A.V. Potapov, M.B. Plotnikov, ,et al.
FSBSI Research Institute of Human Morphology
Introduction. Oxidative stress is a non-specific pathogenetic link in inflammatory diseases of the oral mucosa, which has a destructive effect on its structure and function. In this regard, natural antioxidants such as (p-tyrosol) are of great interest to researchers. The purpose of this paper was to study the effect of p-tyrosol on the morphofunctional changes in the microcirculatory during experimental stomatitis. Materials and methods. The experiment was carried out on 72 Wistar rats with experimental stomatitis. The animals were divided into four groups, each including 18 subjects: the control (intact rats; group 1 with the burn and no treatment (group 2), with the burn and subsequent administration of p-tyrosol (group 3), and with the burn and subsequent administration of Lidocaine + Chamomile flower extract (group 4). Results. The animals of group 3 had the smallest values of the altered vessels, the precapillary diffusion index and the Kernogan index on days 3, 7, and 12. On the contrary, the numerical density of the unchanged arterioles, venules, and capillaries was the greatest. VEGF expression was detected in group 3 on days 7 and 12, and in group 4, on day 12, thus indicating a more intense angiogenesis. Conclusion. On the background of p-tyrosol administration, there is an increased blood supply in the treated area due to the following factors: a growing density of unchanged microvessels, the germination of the ves-sels in the treated area, and induced angiogenesis.

MORPHOLOGICAL PECULIARITIES OF REGENERATION OF ORAL MUCOSA ASSOCIATED WITH USE OF POLYMERIC PIEZOELECTRIC MEMBRANES
AD Koniaeva, EYu Varakuta, AE Leiman, DO Rafiev, EN Bolbasov, and KS Stankevich
Pirogov Russian National Research Medical University
Wound defects of the oral mucosa are a common pathology the treatment of which often involves synthetic membranes. Development of varieties of such membranes is an ongoing process. This study aimed to register morphological features of the oral mucosa regeneration process in the presence of one of the varieties, the polymer piezoelectric membranes. The study involved 45 Wistar rats divided into 3 groups: 1) animals with an open wound defect; 2) animals with a wound defect covered with a copper-coated polymer membrane; 3) intact animals. The samples for morphometric study were collected on the 3rd, 7th and 12th days. On the 3rd day, rats of group 1 had the specific area of granulation tissue 1.4 times greater than that in group 2 (p = 0.033). In group 1 rats, endotheliocytes expressed more VEGF than in the animals of group 2. In group 2, the defect was ultimately completely covered with the epithelial layer, which was not the case in group 1. On the 7th day, the epithelium in rats of group 2 was twice as thick as the layer registered in group 1 (p = 0.019). Granulation tissue was replaced by loose fibrous connective tissue. In group 1, the specific area of inflammatory infiltration was greater than that of loose fibrous connective tissue, and the VEGF expression level was lower than in group 2. On the 12th day, the predominant tissue in group 2 was the loose fibrous connective tissue, the VEGF expression level equaled that of group 3, and peripheral nerves began to grow. In group 1, the specific area of dense fibrous tissue was 3.9 times greater than that in group 2 (p = 0.012), the epithelium had pathological changes and the VEGF expression was below control values. Thus, a polymer piezoelectric membrane had a positive effect on the post-wound restoration of the oral mucosa tissues.

The Specifics of Neovascularization of Wound Defects in the Oral Mucosa during Its Regeneration under a Piezoelectric Polymer Membrane
A. D. Konyaeva, E. Yu. Varakuta, A. E. Leiman, E. N. Bolbasov, and U. V. Chernova
Springer Science and Business Media LLC

Changes in the cellular composition of the inflammatory infiltrate and connective tissue of the oral mucosa in rats during wound healing using a protective piezoelectric coating
A.D. Koniaeva, , E.Y. Varakuta, A.E. Leiman, E.N. Bolbasov, K.S. Stankevich, , , , and
FSBSI Research Institute of Human Morphology
Introduction. Wound healing is a process based on a complex mechanism of intercellular interaction. The aim was to study changes in the oral mucosa cellular composition during wound healing with and without a protective piezoelectric coating. Materials and methods. The study was carried out on 50 Wistar rats divided into four groups: one control group of intact rats and experimental groups 1, 2, and 3. The rats of the experimental groups were subjected to excision of a lip mucous membrane flap with wound formation. In the animals of experimental group 1, the defect was open. In group 2, we used a polymer membrane with copper modification, and in group 3, the membrane was without copper modification. The animals were sacrificed on days 3, 7, and 12 of the study. We used light and electron microscopy to study the qualitative and quantitative changes in the composition of cell populations at the site of the defect. Results. On day 3, there prevailed neutrophilic infiltration in all groups. In groups 2 and 3, we observed a large number of macrophages and fibroblasts that indicated the transition to the next phase of wound healing. On day 7, in group 1, there persisted extensive neutrophilic and macrophage infiltration, whereas, in groups 2 and 3, the signs of inflammation decreased, and wound healing was active. On day 12, in group 1, all values were significantly higher than in the control group and there was damage to the ultrastructure; in groups 2 and 3, all the studied parameters reached the control values. Conclusion. We revealed the patterns of changes in the cellular composition of a wound during its healing. The use of the coating contributed to accelerated wound healing, which was found during the analysis of changes in the composition of cell populations. The closure of the oral mucosa wounds with polymer piezoelectric membranes was proven to have a good effect on tissue repair and was supposed to reduce the risk of postoperative complications. Keywords: wound defect, mucous membrane, oral cavity, piezoelectrics, inflammation, regeneration, scaffolds, dentistry

Restoration of the microvasculature and hemodynamics in the oral mucosa wound defects area with and without a piezoelectric polymer membrane
A.D. Koniaeva, , E.Y. Varakuta, A.E. Leiman, E.N. Bolbasov, K.S. Stankevich, , , , and
FSBSI Research Institute of Human Morphology
Introduction. Restoration of blood supply to tissues is crucial for the regeneration of wound defects. The purpose of this research was to study the effect of piezoelectric polymers on the restoration of microcirculation and hemodynamics in the area of oral mucosa wound defects. Materials and methods. The study was carried out on 60 Wistar rats divided into four groups (15 rats each): control (intact) and 3 experimental groups. In group 1, the animals had an open wound defect. In groups 3 and 2, the rats had a wound defect covered with a membrane with and without copper modification, respectively. The specimens for subsequent light and electron microscopy were collected on days 3, 7, and 12. We studied qualitative and quantitative indicators of microcirculation and hemodynamics restoration. Results. Day 3: in all experimental groups, the arteriolo-venular relationship and the pericapillary diffusion index significantly decreased, while the Kernogan index increased. We also observed a significant increase in VEGF expression. Day 7: in experimental group 3, the values of the Kernogan index and the arteriolo-venular relationship were restored, and the pericapillary diffusion index remained significantly higher than in the control group. In the groups 1 and 2, none of the indicators reached the control values. VEGF expression decreased in all groups. On day 12 in group 1, the arteriolo-venular relationship, the Kernogan index and the index of pericapillary diffusion differed from the control group, in contrast to groups 2 and 3, where all the studied parameters were restored. Expression of VEGF in group 1 was significantly less than the control values, and in groups 2 and 3 it was significantly higher. Conclusion. We revealed that closing of oral mucosa wound defects by a piezoelectric polymer membrane led to the restoration of hemodynamic parameters and promoted active vascular formation. Keywords: wound defect, oral cavity, inflammation, regeneration, microcirculation, neoangiogenesis

Piezoelectric polymer membranes with thin antibacterial coating for the regeneration of oral mucosa
A.D. Badaraev, A. Koniaeva, S.A. Krikova, E.V. Shesterikov, E.N. Bolbasov, A.L. Nemoykina, V.M. Bouznik, K.S. Stankevich, Y.M. Zhukov, I.P. Mishin,et al.
Elsevier BV

Anastasiia Koniaeva

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Scopus Publications