Dr.A.Anton Smith
@annamalaiuniversity.ac.in
Department of Pharmacy
Annamalai University
EDUCATION
M.Pharm., PhD., PGDCA., DIH., DQCM., MISTE., FICS.,
RESEARCH INTERESTS
Pharmaceutical Analysis, Phyto Chemistry, Medicinal Chemistry, Nano Formulation
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Upender Rao Eslawath, Kannappan Kannappan N, Venkateshwarlu Venkateshwarlu L, and Anton Smith A
EManuscript Technologies
Anju A. Varghese, Anton Smith A., and Sreejith M.
Indian Association of Biomedical Scientists
Introduction and Aim: Crude herbal drugs and commercial extracts obtained from plant sources continue to play a significant role in healthcare, particularly in developing countries where traditional medicine practices are deeply rooted. The aim of the study was to estimate the antioxidant (DPPH) and cytotoxic effects of the ethanolic extract of Flaveria trinervia on raw 246.7 cells. Materials and Methods: In the Indian town of Thoothukudi's Kovilpatti village, F. trinervia was isolated. The entire plant was air-dried before being crushed, and 1 kg of the powdered ethanolic extract was employed in the Soxhlet apparatus for continuous extraction. By utilizing GC-MS analysis, F. trinervia ethanolic extract phytochemical assessment was studied. The antioxidant potential of F. trinervia was examined using the DPPH test. Raw 246.7 cell lines were used to test the ethanolic extract of F. trinervia for cytotoxic activity. Results: F. trinervia extracts were found to contain a variety of biologically active phytoconstituents, including ergosterol, octadecanoic acid, propanoic acid, and isopropyl palmitate, according to phytochemical analysis. Compared to the negative control, the ethanolic extract of F. trinervia considerably showed antioxidant activity and cytotoxicity. Ascorbic acid antioxidant activity with DPPH was 98.12%, whereas F.trinervia ethanolic extract's antioxidant activity was 78.91%. The anticancer efficacy of F. trinervia ethanolic extract against uncultured 246.7 cells was evaluated using the MTT assay. By charting cell viability vs extract concentration, the IC50 value was determined. The MTT assay identified raw cells IC50 at 24 hrs as 200 µg/mL of ethanolic F. trinervia extract. Conclusion: Active phytoconstituents are present in the F. trinervia whole plant ethanolic extract. Because of the presence of phytoconstituents, the extracts have excellent antioxidant activity and good cytotoxicity activity against raw 246.7 cell lines. The study recommended using whole plant extract from F. trinervia to treat several metabolic diseases.
N. Hema Rajini and A. Anton Smith
Springer International Publishing
M. Mouli Chandar and A. Anton Smith
Diva Enterprises Private Limited
Ripening of banana fruits was carried out by different methodologies like naturally, stressed by fumes, placing in hot condition, using calcium carbide, calcium chloride, potash, ethylene, ethereal, ethylene glycol, methyl jasmonate, etc. The objective of the study is aimed to review on artificial ripening of banana and its physicochemical changes, benefits and harmful effect caused by ripening methods. Artificial ripening of banana is experimented by fumigation and its results on morphological and physiological changes like colour, sweetness, firmness, acceptability have been observed. Various harmful effects or toxicity which is caused by artificial ripening agents are also studied. This purpose of this article is to showcase the most toxic to least or nil toxic agents and to portray the best acceptable methods of artificial ripening of banana. It was found evidence that natural method of artificial ripening gives beneficial effects without toxicity whereas stress induced or chemically induced artificial ripening leads to changes in active components that may lead to physiological malfunctioning of human beings. It was found that the order of beneficial effect is declined by natural > ethylene > ethrel with sodium hydroxide > methyl jasmonate > ethephon > calcium chloride > ethylene glycol > calcium carbide.
Natarajan VENKATESAN and Arul Gnana Dhas ANTON SMITH
Elsevier BV
AIM
To evaluate the effect of an active fraction from Leptadenia reticulata leaves on serum glucose and lipid profile in normal and diabetic rats.
METHOD
Diabetes was induced by streptozotocin in Wistar rats. Petroleum ether, ethyl acetate, and ethanol extracts of Leptadenia reticulata leaves were administered orally at a dose of 200 mg·kg(-1), p.o. Metformin was used as standard anti diabetic drug (50 mg·kg(-1), p.o). The extract showing higher antidiabetic activity was subjected to column chromatography and led to the isolation of an active fraction, which was given trivial name Lr-1. Lr-1 (100 mg·kg(-1), p.o.) was studied for its hypoglycemic and hypolipidemic potential.
RESULTS
The ethanol extract was found to lower the FBG level significantly (P < 0.05) in diabetic rats. Lr-1 caused a significant (P < 0.05) reduction in FBG level, and additionally it caused reduction in cholesterol, triglyceride levels, and an improvement in the HDL level in diabetic rats.
CONCLUSION
Reduction in the FBG, cholesterol, triglyceride levels, and an improvement in the HDL by Lr-1 indicates that Lr-1 has antidiabetic activity, along with cardioprotective potential, and provides a scientific rationale for the use as an antidiabetic agent.
S. Rajkumari, Madhavan Nirmal, P.M. Sunil, and A. Anton Smith
Elsevier BV
AIM
The quest to identify an accurate method of age estimation, had lead to the evaluation of aspartic acid racemisation in hard tissues of the human remains using high performance liquid chromatography (HPLC). Our study is aimed at the applicability of the racemisation technique to use dentin as the sample to estimate the age in South Indian sub-population.
MATERIALS AND METHODS
Thirty-six non-carious teeth from living individuals distributed among 6 age groups (6 each), sexes (18 each) and jaws (18 each) were analysed for dextro (d) and levo (l) forms of aspartic acid using high performance liquid chromatography (HPLC) technique and their racemisation ratio were calculated for each tooth sample.
RESULTS
High correlation was obtained between the aspartic acid racemisation rates in dentin and age of the individual with an error limited to ±3 years. Racemisation rates in teeth did not significantly differ between the sexes or jaws.
CONCLUSION
The d-aspartic acid accumulation in dentin is synchronous with the aging of an individual and can be used as an accurate method of age estimation in our population.
S. Amuthalakshmi, A. Anton Smith, and R. Manavalan
Bentham Science Publishers Ltd.