Evaluation of Drug-Loaded and Surface-Adsorbed DNase/Tween-80 Solid Lipid Nanoparticles against Staphylococcus aureus Biofilms Sarita Maurya, Manish Gaur, Mohd. Sohail Akhtar, Awadh Bihari Yadav ACS Applied Bio Materials, 2024 The aim of this study was to explore the suitability of Tween-80 or DNase I adsorbed onto the surface of gentamicin-loaded solid lipid nanoparticles (SLNs) to disrupt Staphylococcus aureus biofilms in vitro. We hypothesized that surface-adsorbed DNase I or Tween-80 of SLNs will degrade the biofilm component, extracellular DNA (e-DNA), and extracellular matrix (ECM) of S. aureus biofilms. The SLNs loaded with drug (core) and surface-adsorbed disruptors (Tween-80 or DNase I) to deliver biofilm disruptors first at the site of action, which will help to break down the biofilm, and further drug release from the core will easily penetrate the biofilm and facilitate the killing of bacteria residing in S. aureus biofilms. The SLNs were synthesized by the double emulsion method; the size was 287.3 ± 7.4 nm for blank SLNs and 292.4 ± 2.36 nm for drug-loaded SLNs. The ζ-potential of blank SLNs was -25.6 ± 0.26 mV and that of drug-loaded SLNs was -13.16 ± 0.51 mV, respectively. The successful adsorption of DNase I or Tween-80 was confirmed by the activity of DNase I in blank surface-adsorbed SLNs and the change in the ζ-potential of SLNs after adsorbing DNase I or Tween-80. The surface morphology and size of the SLNs were further characterized using scanning electron microscopy. The encapsulation efficiency of the drug was 16.85 ± 0.84%. The compatibility of the drug with the excipient was confirmed by Fourier transform infrared spectroscopy and the degree of crystallinity was confirmed by X-ray diffraction (XRD) analysis. SLNs showed a sustained release of the drug up to 360 h. SLNs were easily taken up by A549 cells with minimal or no toxicity. The present study showed that Tween-80- or DNase I-adsorbed SLNs efficiently disrupt S. aureus biofilms and possess no or minimal toxicity against cells and red blood cells (RBCs).
Synthesis and Evaluation of BSA-Loaded PLGA-Chitosan Composite Nanoparticles for the Protein-Based Drug Delivery System Manish Gaur, Sarita Maurya, Mohd. Sohail Akhtar, Awadh Bihari Yadav ACS Omega, 2023 The purpose of this study was to synthesize composite nanoparticles (NPs) based on poly(d,l-lactic-co-glycolic acid) (PLGA) and chitosan (CS) and evaluate their suitability for the delivery of protein-based therapeutic molecules. Composite NPs possess a unique property which is not exhibited by any other polymer. Unlike other polymers, only the composite NPs lead to improved transfection efficiency and sustained release of protein. The composite NP were prepared by grafting CS on the surface of PLGA NPs through EDC-NHS coupling reaction. The size of bovine serum albumin (BSA)-loaded PLGA NPs and BSA-loaded PLGA–CS composite NPs was 288 ± 3 and 363 ± 4 nm, respectively. The zeta potential of PLGA NPs is −18 ± 0.23, and that of composite particles is 19 ± 0.40, thus confirming the successful addition of CS on the surface of PLGA NPs. Composite NPs were characterized using dynamic light scattering, scanning/transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, release profile, and gel electrophoresis. The encapsulation efficiency of PLGA NPs was 88%. These composite NPs were easily uptaken by the A549 cell line with no or minimal cytotoxicity. The present study emphasizes that the composite NPs are suitable for delivery of BSA into the cells with no cytotoxicity or very little cytotoxicity, while maintaining the integrity of the encapsulated BSA.
Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis Preeti Yadav, Awadh Bihari Yadav, Preksha Gaur, Vartika Mishra, Zul-I Huma, et al. Biomedicines, 2022 Mastitis is the most devastating economic disease in dairy cattle. Mastitis in dairy cattle frequently occurs during the dry period or during early lactation. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus)are the main causative agents of mastitis in India. S. aureus can form microabscesses in the udder and develop a subclinical form of mastitis. This bacterial property hinders an effective cure during the lactation period. Antimicrobials used for treatments have a short half-life at the site of action because of frequent milking; thereforethey are unable to maintain the desired drug concentration for effective clearance of bacteria. We demonstrated the potential of ciprofloxacin-encapsulated nanocarriersthat can improve the availability of drugs and provide an effective means for mastitis treatment. These drug-loaded nanoparticles show low toxicity and slow clearance from the site of action. Antimicrobial activity against clinical strains of E. coli and S. aureus showed that the zone of inhibition depended on the dose (0.5 mg to 2 mg/mL nanoparticle solution from 11.6 to 14.5 mm and 15 to 18 mm). These nanoparticles showed good antimicrobial activity in broth culture and agar diffusion assay against bacteria.
Nanoparticle and Nanostructure Synthesis and Controlled Growth Methods Vancha Harish, Md Mustafiz Ansari, Devesh Tewari, Manish Gaur, Awadh Bihari Yadav, et al. Nanomaterials, 2022 Nanomaterials are materials with one or more nanoscale dimensions (internal or external) (i.e., 1 to 100 nm). The nanomaterial shape, size, porosity, surface chemistry, and composition are controlled at the nanoscale, and this offers interesting properties compared with bulk materials. This review describes how nanomaterials are classified, their fabrication, functionalization techniques, and growth-controlled mechanisms. First, the history of nanomaterials is summarized and then the different classification methods, based on their dimensionality (0–3D), composition (carbon, inorganic, organic, and hybrids), origin (natural, incidental, engineered, bioinspired), crystal phase (single phase, multiphase), and dispersion state (dispersed or aggregated), are presented. Then, the synthesis methods are discussed and classified in function of the starting material (bottom-up and top-down), reaction phase (gas, plasma, liquid, and solid), and nature of the dispersing forces (mechanical, physical, chemical, physicochemical, and biological). Finally, the challenges in synthesizing nanomaterials for research and commercial use are highlighted.
RNAi in Respiratory Diseases Ciara Kelly, Awadh B. Yadav, Paul J. McKiernan, Catherine M. Greene, Sally-Ann Cryan Advanced Delivery and Therapeutic Applications of Rnai, 2013
Therapeutic aerosol bioengineering for targeted delivery in the lungs 24th European Conference on Biomaterials Annual Conference of the European Society for Biomaterials, 2011
Inhalable microparticles containing isoniazid and rifabutin target macrophages and 'stimulate the phagocyte' to achieve high efficacy Indian Journal of Experimental Biology, 2009
Design, Synthesis, and Biological Evaluation of Tetrahydropyridine Analogues as Potent Antibiofilm Agents against S. aureus : In Vitro and In Silico Studies G Chaudhary, A Verma, S Maurya, R Singh, S Tyagi, B Manna, P Katara, ... ACS Omega , 2025 2025 Citations: 1
Lung SPLUNC1-derived anti-biofilm peptide in polymeric nanoparticles: A novel strategy against S. aureus biofilms and antimicrobial resistance MG S Maurya, R Tripathi, M Pasupuleti, MS Akhtar, S Swaroop, AB Yadav International Journal of Biological Macromolecules , 2025 2025 Citations: 1
Conquering the tuberculosis Superbug: Innovative approaches for biofilm disruption and antibiotic resistance mitigation K Jadhav, S Gautam, AK Singh, RK Verma, AB Yadav Journal of Drug Delivery Science and Technology 111, 107173 , 2025 2025 Citations: 1
Evaluation of antibiofilm and antimicrobial property of Cymbopogon citratus leaves extract fraction against Staphylococcus aureus Biofilm: in-vitro and Insilco approach R Singh, S Maurya, S Tyagi, U Minhas, P Katara, AB Yadav 2024
Effective Cerebral Tuberculosis Treatment via Nose-to-Brain Transport of Anti-TB Drugs Using Muco-Adhesive Nano-aggregates RKVER K Jadhav, A Jhilta, R Singh, V Kumar, A B Yadav, A K Singh Nanoscale , 2024 2024 Citations: 17
Staphylococcus aureus Biofilm Destabilization by Tween-80 and Lung Surfactants to Overcome Biofilm-Imposed Drug Resistance S Maurya, M Gaur, AB Yadav Applied Biochemistry and Biotechnology 196 (3), 1558-1569 , 2024 2024 Citations: 7
Evaluation of Drug-Loaded and Surface-Adsorbed DNase/Tween-80 Solid Lipid Nanoparticles against Staphylococcus aureus Biofilms S Maurya, M Gaur, MS Akhtar, AB Yadav ACS Applied Bio Materials 7 (3), 1501-1512 , 2024 2024 Citations: 5
Cutting-edge advances in tailoring size, shape, and functionality of nanoparticles and nanostructures: A review V Harish, MM Ansari, D Tewari, AB Yadav, N Sharma, S Bawarig, ... Journal of the Taiwan Institute of Chemical Engineers 149, 105010 , 2023 2023 Citations: 381
Synthesis and Evaluation of BSA-Loaded PLGA–Chitosan Composite Nanoparticles for the Protein-Based Drug Delivery System M Gaur, S Maurya, MS Akhtar, AB Yadav ACS omega 8 (21), 18751-18759 , 2023 2023 Citations: 51
Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis P Yadav, AB Yadav, P Gaur, V Mishra, ZI Huma, N Sharma, YO Son Biomedicines 10 (12), 3282 , 2022 2022 Citations: 33
Review on nanoparticles and nanostructured materials: Bioimaging, biosensing, drug delivery, tissue engineering, antimicrobial, and agro-food applications V Harish, D Tewari, M Gaur, AB Yadav, S Swaroop, M Bechelany, ... Nanomaterials 12 (3), 457 , 2022 2022 Citations: 708
Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis. Biomedicines 2022, 10, 3282 P Yadav, AB Yadav, P Gaur, V Mishra, ZI Huma, N Sharma, YO Son s Note: MDPI stays neutral with regard to jurisdictional claims in published … , 2022 2022 Citations: 1
Preparation and optimization of insulin loaded solid lipid nanoparticles for the targeted delivery of insulin to the lungs for diabetes treatment via inhalation P Yadav, AB Yadav Composite Materials for Extreme Loading: Proceedings of the Indo-Korean … , 2021 2021 Citations: 6
for the Targeted Delivery of Insulin to the Lungs for Diabetes Treatment P Yadav, AB Yadav Composite Materials for Extreme Loading: Proceedings of the Indo-Korean … , 2021 2021
Preparation and characterization of BSA as a model protein loaded chitosan nanoparticles for the development of protein-/peptide-based drug delivery system PYAB Yadav Future Journal of Pharmaceutical Sciences 7 , 2021 2021 Citations: 96
Inhalable particles containing isoniazid and rifabutin as adjunct therapy for safe, efficacious and relapse-free cure of experimental animal tuberculosis in one month AKSRKVJKMABYKAKDDGPCRCM AmitMisraa Tuberculosis 128 , 2021 2021 Citations: 21
Biomedical Applications of Carbon Nanomaterials: Fullerenes, Quantum Dots, Nanotubes, Nanofibers, and Graphene M Gaur, C Misra, AB Yadav, S Swaroop, FÓ Maolmhuaidh, M Bechelany, ... Materials 14 (20), 5978 , 2021 2021 Citations: 342
Protein stability and functional activity during nebulization: A comparative study of three nebulizer! N Singh, P Yadav, P Gaur, M Gaur, AB Yadav bioRxiv, 2020.05. 09.085720 , 2020 2020 Citations: 4
Review on nanoparticles and nanostructured materials: Bioimaging, biosensing, drug delivery, tissue engineering, antimicrobial, and agro-food applications V Harish, D Tewari, M Gaur, AB Yadav, S Swaroop, M Bechelany, ... Nanomaterials 12 (3), 457 , 2022 2022 Citations: 708
Cutting-edge advances in tailoring size, shape, and functionality of nanoparticles and nanostructures: A review V Harish, MM Ansari, D Tewari, AB Yadav, N Sharma, S Bawarig, ... Journal of the Taiwan Institute of Chemical Engineers 149, 105010 , 2023 2023 Citations: 381
Biomedical Applications of Carbon Nanomaterials: Fullerenes, Quantum Dots, Nanotubes, Nanofibers, and Graphene M Gaur, C Misra, AB Yadav, S Swaroop, FÓ Maolmhuaidh, M Bechelany, ... Materials 14 (20), 5978 , 2021 2021 Citations: 342
Inhalable microparticles containing large payload of anti-tuberculosis drugs P Muttil, J Kaur, K Kumar, AB Yadav, R Sharma, A Misra European Journal of Pharmaceutical Sciences 32 (2), 140-150 , 2007 2007 Citations: 234
Intracellular time course, pharmacokinetics, and biodistribution of isoniazid and rifabutin following pulmonary delivery of inhalable microparticles to mice RK Verma, J Kaur, K Kumar, AB Yadav, A Misra Antimicrobial agents and chemotherapy 52 (9), 3195-3201 , 2008 2008 Citations: 122
Uptake of inhalable microparticles affects defence responses of macrophages infected with Mycobacterium tuberculosis H37Ra R Sharma, P Muttil, AB Yadav, SK Rath, VK Bajpai, U Mani, A Misra Journal of antimicrobial chemotherapy 59 (3), 499-506 , 2007 2007 Citations: 114
A hand-held apparatus for “nose-only” exposure of mice to inhalable microparticles as a dry powder inhalation targeting lung and airway macrophages J Kaur, P Muttil, RK Verma, K Kumar, AB Yadav, R Sharma, A Misra European Journal of Pharmaceutical Sciences 34 (1), 56-65 , 2008 2008 Citations: 103
Preparation and characterization of BSA as a model protein loaded chitosan nanoparticles for the development of protein-/peptide-based drug delivery system PYAB Yadav Future Journal of Pharmaceutical Sciences 7 , 2021 2021 Citations: 96
Synthesis and Evaluation of BSA-Loaded PLGA–Chitosan Composite Nanoparticles for the Protein-Based Drug Delivery System M Gaur, S Maurya, MS Akhtar, AB Yadav ACS omega 8 (21), 18751-18759 , 2023 2023 Citations: 51
Inhalable Microparticles Containing Isoniazid and Rifabutin Target Macrophages and “Stimulate the Phagocyte” to Achieve High Efficacy AB Yadav, R Sharma, P Muttil, AK Singh, RK Verma, M Mohan, SK Patel, ... Indian J Exp Biol 47 (6), 469-74 , 2009 2009 Citations: 39
Microparticles induce variable levels of activation in macrophages infected with Mycobacterium tuberculosis AB Yadav, P Muttil, AK Singh, RK Verma, M Mohan, AK Agrawal, ... Tuberculosis 90 (3), 188-196 , 2010 2010 Citations: 37
Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis P Yadav, AB Yadav, P Gaur, V Mishra, ZI Huma, N Sharma, YO Son Biomedicines 10 (12), 3282 , 2022 2022 Citations: 33
Enhancement of apoptosis of THP-1 cells infected with Mycobacterium tuberculosis by inhalable microparticles and relevance to bactericidal activity AB Yadav, A Misra Antimicrobial agents and chemotherapy 51 (10), 3740-3742 , 2007 2007 Citations: 33
Therapeutic Aerosol Bioengineering of siRNA for the Treatment of Inflammatory Lung Disease by TNFα Gene Silencing in Macrophages C Kelly, AB Yadav, C Lawlor, K Nolan, J O’Dwyer, CM Greene, ... Molecular pharmaceutics 11 (11), 4270-4279 , 2014 2014 Citations: 24
Inhalable microparticles modify cytokine secretion by lung macrophages of infected mice R Sharma, AB Yadav, P Muttil, H Kajal, A Misra Tuberculosis 91 (1), 107-110 , 2011 2011 Citations: 24
The devil’s advocacy: When and why inhaled therapies for tuberculosis may not work AB Yadav, AK Singh, RK Verma, M Mohan, AK Agrawal, A Misra Tuberculosis 91 (1), 65-66 , 2011 2011 Citations: 23
Inhalable particles containing isoniazid and rifabutin as adjunct therapy for safe, efficacious and relapse-free cure of experimental animal tuberculosis in one month AKSRKVJKMABYKAKDDGPCRCM AmitMisraa Tuberculosis 128 , 2021 2021 Citations: 21
DNA nanostructures: chemistry, self-assembly, and applications A Sharma, K Vaghasiya, RK Verma, AB Yadav Emerging Applications of Nanoparticles and Architecture Nanostructures, 71-94 , 2018 2018 Citations: 20
Effective Cerebral Tuberculosis Treatment via Nose-to-Brain Transport of Anti-TB Drugs Using Muco-Adhesive Nano-aggregates RKVER K Jadhav, A Jhilta, R Singh, V Kumar, A B Yadav, A K Singh Nanoscale , 2024 2024 Citations: 17
