Dr. Azharuddin Daphedar
@anjuman arts, science and commerce college, vijayapur
Anjuman Arts, Science and Commerce College, Vijayapur
EDUCATION
M.Sc. Ph.D.
RESEARCH, TEACHING, or OTHER INTERESTS
Environmental Science, General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, Biotechnology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Azharuddin B. Daphedar, Sanjay S. Majani, Praveenkumar J. Kaddipudi, Raveendra B. Hujaratti, Siddappa B. Kakkalmeli, Ali A. Shati, Mohammad Y. Alfaifi, Serag Eldin I. Elbehairi, Chandan Shivamallu, Usha Jinendra,et al.
Elsevier BV
Azharuddin B. Daphedar, Siddappa Kakkalameli, Basheerabegum Faniband, Muhammad Bilal, Ram Naresh Bhargava, Luiz Fernando Romanholo Ferreira, Abbas Rahdar, Dummi Mahadevan Gurumurthy, and Sikandar I. Mulla
Springer Science and Business Media LLC
Chandrashekar Srinivasa, SR Santosh Kumar, Sushma Pradeep, Shashanka K Prasad, Ravindra Veerapur, Mohammad Azam Ansari, Mohammad N Alomary, Saad Alghamdi, Mazen Almehmadi, Kavitha GC,et al.
Informa UK Limited
Introduction Cancer disease is known due to its unregulated proliferation of cells that have evolved from the body’s regular cells. The disease develops as a result of epigenetic and genetic modifications, tumor suppressor gene inactivation, and oncogene activation. The present work describes an environmentally benign approach for the synthesis of manganese oxide nanoparticles (MnO2 NPs) using Gmelina arborea fruit extract (GAE) in an aqueous medium. Methods The study evaluated the formation of MnO2 NPs and their anticancer efficacy against MCF-7 breast cancer cell line. Results The formation of MnO2 NPs was confirmed through powder X-ray diffractometer (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HR-TEM). The crystalline nature of as-prepared MnO2 NPs was evident from XRD pattern. The morphology of the material was studied using SEM analysis, which suggested a rod-like nature with an average diameter of 50 nm. Further, the TEM and HR-TEM images confirmed the rod shape of the as-prepared MnO2 NPs with an interplanar distance of 0.271 nm. In addition, the concentric rings from selected area electron diffraction (SAED) analysis show the crystalline nature of the as-prepared material, which further supports the obtained XRD pattern. The anticancer efficacy of MnO2 NPs was evaluated against MCF-7 breast cancer cell line, which showed up to 96% inhibition of the cells at 400 µg/mL concentration. Conclusion Bio-conjugation of MnO2 NPs can provide enough scope for the therapeutic use of Gmelina arborea, assuming appropriate mechanistic evaluations are conducted.
Azharuddin Daphedar and Tarikere C. Taranath
Firenze University Press
Abstract The present investigation was undertaken to evaluate the genotoxic effect of zinc nanoparticles (ZnNPs) synthesized by leaf extract of Albizia saman on mitotic chromosomes of root tip meristematic cells of Drimia indica. Biogenic ZnNPs were characterized by using UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), atomic force microscopy (AFM) and high resolution electron microscopy (HR-TEM) analyses. Root tips of D. indica were exposed to different concentrations of ZnNP suspension, namely 4, 8, 12 and 16 μg ml–1, for 24 h at intervals of 6, 12, 18 and 24 h. The meristematic cells were analyzed for mitotic index and chromosomal aberrations such as chromosomal bridges, sticky chromosomes, multibridge anaphase, laggards, diagonal anaphase, lagging chromosomes, and c-metaphase as well as for the induction of micronuclei. It is evident from the data that the mitotic index is inversely proportional and chromosomal aberrations are directly proportional to the concentration and duration of exposure to ZnNPs. The data were found to be significant at p˂0.05. It is concluded that biogenic ZnNPs induce chromosomal abnormality in root tip meristematic cells of D. indica.
Azharuddin Daphedar and Tarikere C. Taranath
Elsevier BV
Azharuddin Daphedar and Tarikeri C. Taranath
Springer Science and Business Media LLC