Begum fauziyah
@uin-malang.ac.id
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Roihatul Mutiah, Malich Septi Diajeng Briliana, Ananda Rizkia Azizah Ahmad, Begum Fauziyah, Nandana Adyuta Janaloka, and Arief Suryadinata
ARTS Publishing
Side effects and risk of resistance are common consequences of colon cancer treatment based on chemotherapy. The medicinal plant originating in Indonesia, Lansium parasiticum bark extract (LPBE), has not been studied much. The purpose of this study is to identify the compounds present in LPBE and explain how the molecular mechanisms of the composite inhibit colon cancer cells. LC-MS/MS Liquid Chromatography Tandem Mass Spectrophotometry has been used to identify compounds in LPBE. The ADMET program is used to determine absorption profiles and bioavailability per oral. The tissue pharmacology approach uses Cytoscape 3.9.1, GeneCards, Disgenet, STRING 2.0.0, SRplot, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway to predict the molecular anti-cancer mechanisms of these compounds. In vitro validation using PyRx Autodock Vina 9.0 and Biovia Discovery Studio with BAX (PDB ID:2YXJ), BCL2 (P DB ID:2W3L) and STAT3 receptors (PDB ID:6NJS). A total of 17 active compounds were identified through LC-MS/MS. The moronic acid compound showed the highest levels of 14.29% followed by 4-Morpholineacetic Acid 12.2% and ursolic aldehyde 8.37%. Pharmacological network analysis showed that the compounder works on the EGFR tyrosine kinase resistance path by targeting the BCL2, BAX, STAT3 genes. The results of the in silico validation support the results of tissue pharmacology findings. Ursolic aldehyde, and Moronic acid showed a higher affinity to the three receptors. Therefore, Lansium parasiticum bark extract (LPBE) is recommended for further study as a candidate anti-cancer drug both in vitro and in vivo.
Begum Fauziyah, Dyah Fatimatussholichah, and Aldila Syarifah
Egypts Presidential Specialized Council for Education and Scientific Research
B. Fauziyah, Dyah Fatimatussholichah, Aldila Syarifah and Burhan Ma’arif
Gelatin is a clear, flavorless, and colorless biopolymer formed by the denaturation of collagen protein in animal tissues. Gelatin is an important ingredient in the manufacture of capsule shell films. The tensile strength and elongation values of gelatin film indicate its quality. Sucrose is one of the ingredients that can affect the tensile strength and elongation of the gelatin film. To determine the effect of adding sucrose on the tensile strength and elongation of the chicken leg skin gelatin film. To extract the gelatin, clean, sliced chicken leg skin was freeze-dried, ground into powder, and extracted. The characteristics of chicken skin gelatin were measured using Fourier-transform infrared, and the data were read in the 4000–400 cm − 1 range for 32 scans. Pouring the solution into a mold, flattening it, and drying it at 40°C results in a film that has been tested for tensile strength and elongation. Gelatin films with a 30% sucrose addition had the best tensile strength and elongation values, with a tensile strength of 3.03 MPa and an elongation of 152.02%. Sucrose alters the tensile strength and elongation of chicken leg gelatin film. 30% sucrose provides better tensile and elongation strength.
Begum Fauziyah, Asna Defi Batrisyia, Alif Firman Firdausy, Burhan Ma’arif, and Siti Maimunah
Oriental Scientific Publishing Company
Dates (Phoenix dactylifera L.) are a unique fruit because they have many health benefits. Ajwa dates are one of the dates that many people are interested in for ingestion. Making date-infused water or nabeez water is one technique to ingest dates as exemplified by the Prophet SAW and is believed to have many benefits for digestion. As a result, there has never been any research on the analysis of active compounds from nabeez water, particularly Ajwa dates themselves, so the goal of this study was to determine the content of active compounds in nabeez water as well as the total content based on qualitative and quantitative analysis. The nabeez water from dates was tested qualitatively and quantitatively using phytochemical tests and UV-Visible Spectrophotometry. The results show that the nabeez water contains active ingredients of flavonoids, saponins, alkaloids, and tannins, according to qualitative analytical results. The quantitative analysis showed that nabeez water has a total concentration of flavonoid compounds of 5.749 g/mL and a concentration of tannin compounds of 52.934 g/mL.
Burhan Ma’arif, Fariza Amanatul Sholihah, Anisah Mahardiani, Begum Fauziyah, Denis Mery Mirza, and Mangestuti Agil
Oriental Scientific Publishing Company
Estrogen deficiency can contribute to osteoporosis in postmenopausal women. Phytoestrogens are becoming more widely recognized as potential estrogen replacement therapy. The administration of phytoestrogens can cause bone formation, which is marked by an increase in Runx2 expression in osteoblast cells and can be seen using western blot and immunohistochemistry approaches. This review aimed to compare the detection methods of Runx2 in phytoestrogen-induced bone tissue using western blots and immunohistochemistry. Selectivity, sensitivity, processing time, and cost-effectiveness were the parameters that were compared. This review was done by identifying articles in several databases (Google Scholar, PubMed, and Science Direct). The process of selecting the articles used the PRISMA guidelines to create a flowchart with inclusion and exclusion study criteria. Meta-synthesis was done to analyze, identify, and interpret all of the data in the articles systematically. 70 articles in total were obtained from the selection process, with 21 articles being relevant to the topic. The result shows that the selectivity and sensitivity of western blot for detecting Runx2 on tissue were 93.5–100%, respectively, whereas immunohistochemistry selectivity and sensitivity were 45–99.5%, respectively. Compared to immunohistochemistry, western blot can save up to 57.26%. Immunohistochemistry takes 46 hours to process, while Western blot takes 25 hours and 20 minutes. In comparison to immunohistochemistry, the western blot is more selective, sensitive, rapid and affordable for detecting Runx2 in bone tissue.
B Fauziyah, M Yuwono, and Isnaeni
IOP Publishing
Abstract Saccharum officinarum L. bagasse is waste from sugarcane plants contains lignin, hemicellulose, cellulose and wax compounds. Isolation of cellulose from sugarcane can be done by acid hydrolysis of sugarcane waste and delignification with Sodium Hydroxide (NaOH). The objectives of this research were to study the effect of acid type and concentration on the physical and chemical characteristics of cellulose isolated from sugarcane bagasse. The acid variation that used were nitric acid: sulphuric acid; nitric acid; hydrochloric acid and sulphuric acid: hydrochloric acid in 1:1; 2:1 and 3:1 in the variation of concentration for each combination. Cellulose characteristics observed were color, shape and powder properties such as Hausner Ratio, Carr’s index, Angle of Repose and functional group analysis of cellulose using FTIR (Fourier Transform Infrared). The cellulose that isolated from sugarcane bagasse with HNO3: H2SO and HNO3: HCl were matched in white powder form with standard cellulose. Cellulose color that obtained with other acids were cream in fiber form. The results showed that the hydrolysis process of cellulose with nitric acid was more effective than sulfuric acid and hydrochloric acid. Standard cellulose FTIR spectra showed that delignification process was success and cellulose obtained from sugarcane bagasse. Sugarcane bagasse cellulose samples that isolated with nitric acid: hydrochloric acid (2:1) was the largest in percentage of cellulose (44.143%) and showed in line peaks with standard cellulose. Bulk Densities, Tapped Densities, Hausner Ratio, Carr’s index and Angle of Repose values of sugarcane bagasse cellulose isolated in sequence were 0.05; 0.07; excellent; 26.6; good.
M Yuwono, B Fauziyah, Isnaeni, and A Nisak
IOP Publishing
Abstract Nano cellulose was successfully isolated from sugarcane bagasse through sulphuric acid hydrolysis of sugarcane bagasse cellulose. Physically, Nano cellulose was transparent and broken white. The crystallinity index of sugarcane bagasse nano cellulose was 80.72%. The particle size of sugarcane bagasse nano cellulose was 225 nm. Delignification process in isolation was successfully showed by releasing peaks in 1239.3 and 1507.7. It show C-O-C vibration of aryl group in lignin and C=C aromatic ring in lignin respectively. Sugarcane bagasse cellulose shows peaks at 1720.2 that represent COOH and hemicellulose carboxylic groups, while the others were not found. The crystallinity index of Nano cellulose was 42.65%. Nano cellulose film prepared in several concentrations (3%, 6%, and 9%). Nano cellulose film also prepared with adding HPMC 2%. Nano cellulose film prepared in 9% concentration was too strict and broken easily. The tensile strength and elongation of Nano cellulose film that prepared in 3% + HPMC 2% and 6% + HPMC 2% were 3.177 Mpa, 10.93% and 3.315 Mpa dan 3.7% respectively.