Vaibhav Bhatt
@gtu.ac.in
Director, School of Applied Sciences and Technology
Gujarat Technological University
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Aelvish D. Padariya, Nirbhay K. Savaliya, Milan P. Dhaduk, Ravi A. Dabhi, Bhupesh S. Bhatt, Vaibhav D. Bhatt, and Mohan N. Patel
Elsevier BV
Parthkumar Prajapati, Riya Desai, Mamta Varma, Ketankumar Panchal, Subhash Jakhesara, Prakash Koringa, Vaibhav Bhatt, Neelam Nathani, and Chandrashekar Mootapally
Springer Science and Business Media LLC
Milan P. Dhaduk, Ravi A. Dabhi, Vaibhav D Bhatt, Bhupesh S. Bhatt, and Mohan N. Patel
Elsevier BV
Milan P. Dhaduk, Ravi A. Dabhi, Bhupesh S. Bhatt, Vaibhav D. Bhatt, and Mohan N. Patel
Elsevier BV
Nandan C. Pomal, Keyur D. Bhatt, Dinesh S. Kundariya, Riya A. Desai, Vaibhav Bhatt, and Anita Kongor
Wiley
Harsh C. Patel, Manan S. Patel, Jaydeepkumar N. Parekh, Dipakkumar D. Chudasama, Priyanka Dalwadi, Anju Kunjadiya, Vaibhav Bhatt, Krunal M. Modi, Chirag N. Patel, and Kesur R. Ram
Informa UK Limited
Diversely functionalized pyrazolo-pyridine fused tetrazolo-pyrimidines 10aa-am and 10ba-bn were successfully synthesized via a catalyst-free synthetic protocol with moderate to very good yields. The compounds were evaluated for cytotoxicity against MCF-7 and HEK-293 cells using MTT assay. Among the tested compounds, 10ab (IC50- 23.83 µM) and 10ah (IC50- 23.30 µM) demonstrated the highest potency against MCF-7 cells, while 10bc (IC50- 14.46 µM) and 10bh (IC50- 2.53 µM) exhibited excellent cytotoxicity against HEK-293 cells. Additionally, antibacterial screening was performed against three Gram-negative bacteria (E. coli, P. aeruginosa, and S. enterica) and three Gram-positive bacteria (S. aureus, B. megaterium, and B. subtilis) using broth dilution method, while antifungal activity was assessed against three fungal strains (A. niger, Penicillium, and S. cerevisiae) using agar well diffusion method. In antimicrobial screening, the majority of the compounds demonstrated significant antibacterial efficacy compared to antifungal activity. We also conducted comprehensive computational studies, including DFT calculations, molecular docking and dynamics, and drug-likeness assessments. In the DFT study, compounds 10ac and 10bc displayed stable conformations, indicating their potential for higher therapeutic activity. Molecular docking analyses revealed compelling interactions, with compound 10ah demonstrating docking score -7.42 kcal/mol against catalytical domain PARP1 (PDB ID: 7KK4) and 10bh exhibiting a best docking score -10.77 kcal/mol against human corticotropin-releasing factor receptor 1 (PDB ID: 4Z9G). A 100 ns molecular dynamics (MD) simulation study of compounds 10ah and 10bh revealed the stable conformation and binding energy in a stimulating environment. In drug-likeness assessments, both the compounds 10ah and 10bh adhere all the established guidelines.Communicated by Ramaswamy H. Sarma.
Mukesh N. Kher, Sandip P. Dholakia, Dipen K. Sureja, Vaibhav D. Bhatt, and Nirav V. Patel
Elsevier
Ravi A. Dabhi, Milan P. Dhaduk, Vaibhav D. Bhatt, and Bhupesh S. Bhatt
Informa UK Limited
Abstract Series of spiro quinoxaline-β-lactam based heterocyclic compounds (QL 1 – QL 21) were synthesized and characterized by spectroscopic techniques like 1H-NMR, LC-MS, FT-IR spectroscopy and elemental analysis. The binding mode and binding strength between compounds and calf thymus-DNA were estimated by UV–visible spectroscopy, viscosity measurement and molecular docking studies. The compounds bind with the DNA through partial intercalation mode. In the absorption titration experiment, the K b values for all the synthesized compounds were found in the range of 0.24–0.64 × 105 M−1. The protein binding studies of all the synthesized compounds were evaluated by absorption titration experiment, and the K b value for all the compounds was obtained in the range of 0.030–1.571 × 104 M−1. The compounds were screened against two Gram (+ve) and three Gram (–ve) bacteria for antimicrobial activity. The MIC values for all the synthesized compounds were found in 95–255 µM. The LC50 values (cytotoxicity) of the synthesized compounds (QL 1–QL 21) were found in the range of 4.00–12.89 µg/mL. The ADME study was carried out using the online platform SwissADME and admetSAR to evaluate the pharmacokinetic profile of all the synthesized compounds. All the compounds were screened for anticancer activity against the human osteosarcoma (MG-63) cell line. The result shows that all the compounds exhibit effective anticancer activity. Communicated by Ramaswamy H. Sarma
Priya Patel, Mihir Raval, Aneka Manvar, Vishal Airao, Vaibhav Bhatt, and Pranav Shah
Public Library of Science (PLoS)
Silibinin (SB) is shown to have an anticancer properties. However, its clinical therapeutic effects have been restricted due to its low water solubility and poor absorption after oral administration. The aim of this study was to develop SB-loaded PCL/Pluronic F68 nanoparticles for pulmonary delivery in the treatment of lung cancer. A modified solvent displacement process was used to make nanoparticles, which were then lyophilized to make inhalation powder, Nanoparticles were characterized with DSC, FTIR,SEM and In vitro release study. Further, a validated HPLC method was developed to investigate the Biodistribution study, pharmacokinetic parameters. Poly Caprolactone PCL / Pluronic F68 NPs showed the sustained release effect up to 48 h with an emitted (Mass median Aerodynamic diameter)MMAD and (Geometric size distribution)GSD were found to be 4.235 ±0.124 and 1.958±1.23 respectively. More specifically, the SB Loaded PCL/Pluronic F 68 NPs demonstrated long circulation and successful lung tumor-targeting potential due to their cancer-targeting capabilities. SB Loaded PCL/Pluronic F68 NPs significantly inhibited tumour growth in lung cancer-induced rats after inhalable administration. In a pharmacokinetics study, PCL/ Pluronic F68 NPs substantially improved SB bioavailability, with a more than 4-fold rise in AUC when compared to IV administration. These findings indicate that SB-loaded PCL/PluronicF68 nanoparticles may be a successful lung cancer therapy delivery system.
Mukesh N. Kher, Sandip P. Dholakia, Grisha G. Shah, Dipen K. Sureja, Vaibhav D. Bhatt, and Devang B. Sheth
Informa UK Limited
Abstract Vitamins are pivotal ingredients in dietary supplements and nutraceutical products. Since many vitamins are unstable and rapidly degraded, it’s crucial to keep track of their loss throughout processing and storage. Monitoring hydrophilic vitamins may be more crucial than monitoring those lipophilic vitamins because the body does not maintain them as well. There seems to be a need for an all-encompassing method to estimate hydrophilic vitamins. In this study, we have developed a high-performance thin layer chromatography method for estimation of four water-soluble vitamins simultaneously using HPTLC silica-gel 60GF254 plates and ethyl acetate: methanol: hydrochloric acid (0.1N) (8.5:1:0.5 v/v/v) as a mobile phase, which was successfully validated as per the ICH guideline. All the validation parameters were within the acceptable range established by the ICH guideline. The method is precise, reproducible, easy, reliable, and applicable for the estimation of water-soluble vitamins in marketed products. To best of our knowledge, it is simpler, less time-consuming, and more economical than other approaches that have been published for the same purposes GRAPHICAL ABSTRACT
Bhumi R. Rajguru and Vaibhav D. Bhatt
Korean Society of Breeding Science
Manan S. Patel, Jaydeepkumar N. Parekh, Dipakkumar D. Chudasama, Harsh C. Patel, Priyanka Dalwadi, Anju Kunjadiya, Vaibhav Bhatt, and Kesur R. Ram
American Chemical Society (ACS)
A simple, straightforward, and energy-efficient greener route for the synthesis of a series of biologically interesting functionalized 1,1-dihomoarylmethane scaffolds has been developed in the presence of meglumine as an efficient and eco-friendly organo-catalyst via one-pot pseudo-three-component reaction at room temperature. Following this protocol, it is possible to synthesize 1,1-dihomoarylmethane scaffolds of an assortment of C–H activated acids such as dimedone, 1,3-cyclohexadione, 4-hydroxy-6-methyl-2-pyrone, 4-hydroxycoumarin, and 1-phenyl-3-methyl-pyrazolone. The salient features of the present green protocol are mild reaction conditions, good to excellent yields, operational simplicity, easy isolation of products, no cumbersome post treatment, high atom economy, and low E-factor. In addition, this chemistry portrays several green advantages including the reusability of reaction media and product scalability, which makes protocol sustainably efficient. Additionally, several control experiments such as protection of catalyst reactive site, D2O exchange, and 1H NMR studies revealed possible pathways for meglumine-promoted reactions. Inspired by the natural physiological environment of 1,1-dihomoarylmethane scaffolds, we reconnoitered the biological profile of our compounds and synthesized compounds that were promising for their antiproliferative and antibacterial activities.
Ravi A. Dabhi, Milan P. Dhaduk, Vaibhav D. Bhatt, and Bhupesh S. Bhatt
Elsevier BV
Milan P. Dhaduk, Ravi A. Dabhi, Bhupesh S. Bhatt, Vaibhav D Bhatt, and Mohan N. Patel
Elsevier BV
Tushar Patel, Navneet Chauhan, Vaibhav D. Bhatt, and Bhupesh S. Bhatt
Elsevier BV
Jigna Vadalia, Jalpa Sanandia, Vaibhav Bhatt, and Navin Sheth
Bentham Science Publishers Ltd.
Background: Malaria is a major disease in developing countries, and the main issue is its control of resistance against all available anti-malarial drugs. Our aim was to find affordable therapy for this global problem. The present study was conducted to evaluate the antiplasmodial and cytotoxic activities of Euphorbia hirta and Euphorbia thymifolia. Methods: The antiplasmodial activity was evaluated against chloroquine-sensitive (MRC-2), and chloroquine-resistant (RKL-9) strains of Plasmodium falciparum through schizont maturation inhibition and Plasmodium lactate dehydrogenase (PfLDH) activity assays. The cytotoxicity was performed by MTT assay on the Huh7 liver carcinoma cell line. Results: IC50 for PfLDH activity of E. hirta and E. thymifolia methanol extract against P. faciparum MRC-2 strain was 9.6 and 12.6 μg/mL, respectively, and against P. faciparum RKL-9 strain was 10.2 and 11.56 μg/mL, respectively. Methanol extract of E. thymifolia showed lower IC50 (19.3 μg/mL) than methanol extract of E. hirta (24.7 μg/mL) in the MTT assay against Huh7 cell line. Conclusion: Both the plant methanol extracts showed a two-fold lower cytotoxic activity against hepatocyte-derived carcinoma cell line (Huh7) compared to in vitro inhibitory activity against P. falciparum strains (MRC-2 and RKL-9).
Priya Patel, Mihir Raval, Vishal Airao, Vaibhav Bhatt, and Pranav Shah
Informa UK Limited
Abstract Aim In the current study, efforts are being made to prepare Inhalable Silibinin loaded solid lipid nanoparticles (SLNs) with narrow size distribution with improved bioavailability. Methods SLNs were formulated by high shear homogenisation method SLNs were characterised, including Differential Scanning Calorimetry (DSC), Fourier transform infra-red spectroscopy (FTIR), particle size analysis, entrapment efficiency with Aerodynamic behaviour. The MTT assay was performed against A549 cell line, to measure their anticancer cell activity with In vivo study. Results Optimized formulation exhibited spherical surface with a mean particle size of 221 ± 1.251 nm, PI of 0.121 ± 0.081, zeta potential of −4.12 ± 0.744. Aerodynamic behaviour such as Mass median aerodynamic diameter (MMAD) and Geometric size distribution (GSD) were found to be 5.487 ± 0.072 and 2.321 ± 0.141 respectively proved formulation is suitable for inhalation. In vitro cellular efficacy against A549 cells, revealed that the optimised formulations were more effective and potent. Conclusion The Inhalable SLNs approach was successfully engineered and administered to the lungs safely without causing any problems.
Ankita Goswami, Nirav Patel, Vaibhav Bhatt, Mihir Raval, Madhavi Kundariya, and Navin Sheth
Elsevier BV
Jay Nimavat, Chandrashekar Mootapally, Neelam M. Nathani, Devyani Dave, Mukesh N. Kher, Mayur S. Mahajan, Chaitanya G. Joshi, and Vaibhav D. Bhatt
Frontiers Media SA
Humankind has suffered many pandemics in history including measles, SARS, MERS, Ebola, and recently the novel Coronavirus disease caused by SARS-CoV-2. As of September 2021, it has affected over 200 million people and caused over 4 million deaths. India is the second most affected country in the world. Up to this date, more than 38 Lakh viral genomes have been submitted to public repositories like GISAID and NCBI to analyze the virus phylogeny and mutations. Here, we analyzed 2349 genome sequences of SARS-CoV-2 submitted in GISAID by a single institute pertaining to infections from the Gujarat state to know their variants and phylogenetic distributions with a major focus on the spike protein. More than 93% of the genomes had one or more mutations in the spike glycoprotein. The D614G variant in spike protein is reported to have a very high frequency of >95% globally followed by the L452R and P681R, thus getting significant attention. The antigenic propensity of a small peptide of 29 residues from 597 to 625 of the spike protein variants having D614 and G614 showed that G614 has a little higher antigenic propensity. Thus, the D614G is the cause for higher viral antigenicity, however, it has not been reported to be effective to be causing more deaths.
Mihir Raval, Priya Patel, Vishal Airao, Vaibhav Bhatt, and Navin Sheth
Springer Science and Business Media LLC
Prashakha J. Shukla, Vaibhav D. Bhatt, Jayaraman Suriya, and Chandrashekar Mootapally
Wiley
Mukesh Kher, Vaibhav Bhatt, Aashka Jani, and Navin Sheth
Informa UK Limited
Abstract Two stability-indicating chromatographic methods namely, reverse-phase high-performance liquid chromatography (RP-HPLC) and high-performance thin-layer chromatography (HPTLC) were developed and validated for the determination of azilsartan medoxomil from pharmaceutical dosage form in presence of its degradation products as per ICH guideline. In RP-HPLC, separation was performed with Hiber® C-18 column (250 mm X 4.6 mm, 5 μm), using mobile phase methanol: acetonitrile: water (88:8:4 v/v/v) at a flow rate of 0.5 ml/min. The analyte was detected at 254 nm over a concentration range of 20 - 70 μg/ml with correlation coefficient of 0.999. In HPTLC, separation was carried out with silica gel G60 F254 aluminum sheet using acetone: toluene: ammonia (8.2:1.7:0.1 v/v/v) as a developing system. Linearity of the proposed HPTLC method was found 500 - 900 ng/band and correlation coefficient 0.9970 at 254 nm. Besides, stress degradation study was also carried out wherein degraded product peaks were well resolved from the analyte peak with difference in their retention time and retardation factor in RP-HPLC and HPTLC methods respectively. Statistical analysis proved that there were no significant differences between the two validated methods. The proposed stability-indicating methods can be applied for the analysis of azilsartan medoxomil in pharmaceutical formulations for quality control. GRAPHICAL ABSTRACT
Mukesh N. Kher, Navin R. Sheth, and Vaibhav D. Bhatt
Informa UK Limited
ABSTRACT The extent of subclinical mastitis in three breeds of cattle, Kankrej, Gir, and Crossbred, was performed at cattle farms in Anand town of Gujarat State, India. The prevalence of subclinical mastitis in crossbred cattle was higher compared to local breed of cattle. Causative agents identified using 16S rDNA polymerase chain reaction (PCR)-based molecular method were Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Bacillus megaterium. In vitro antibacterial activity of ethyl acetate extract of plant Terminalia chebula (Combretaceae) was checked by agar well diffusion method against four isolated and molecularly identified microorganisms. Ethyl acetate extract shows antimicrobial activity with varying magnitudes against all identified isolates. Among the three different concentrations, 500 µg/mL conc. of extract is as effective as that of standard amoxicillin. In vitro results support the use of plant extract from T. chebula as an alternative to antibiotics therapy against bovine subclinical mastitis.
Vaibhav D. Bhatt, Monika Patel, and Chaitanya G. Joshi
Springer Singapore
Manisha R. Sajnani, D. Sudarsanam, Ramesh J. Pandit, Tejas Oza, Ankit T. Hinsu, Subhash J. Jakhesara, Siddhardha Solosanc, Chaitanya G. Joshi, and Vaibhav D. Bhatt
Elsevier BV