MOHAN GANDHI BONTHU

RESEARCH, TEACHING, or OTHER INTERESTS

Pharmaceutical Science, Pharmaceutical Science

Scopus Publications

Review on Exploring Role of Vitamin D on Alzheimer’s Disease: Mechanistic Insights and Implications
Nagaraju Bandaru, Mohan Gandhi Bonthu, Alluri Pavani Gayatri, Shashikala Metri, Perli Kranti Kumar, Aneesha Addanki, Srilaksmi Nallapaty, Karri Swathi Priya, Dodda Tulase Nadhreddy, and P. N. S. Gowravi
SAGE Publications
Background Alzheimer’s disease (AD) is a complex neurological disorder characterized by neuronal loss and progressive cognitive decline. The disease progression is influenced by both environmental and genetic factors. Recent research highlights the significant role of vitamin D in the pathological progression of AD. Purpose This review aims to comprehensively analyze the interplay between vitamin D and Alzheimer’s disease, focusing on its molecular mechanisms, epidemiological evidence, and therapeutic implications. Methods An extensive literature review was conducted to explore the molecular pathways by which vitamin D affects key pathological processes in AD, including amyloid-beta deposition, oxidative stress, neuronal inflammation, and tau phosphorylation. Epidemiological studies linking vitamin D status with AD prevalence and clinical outcomes were also analyzed, along with data from research trials investigating the efficacy of vitamin D supplementation in AD prevention and management. Results Vitamin D deficiency is associated with increased cognitive decline and heightened risk of developing AD. The review highlights the therapeutic potential of vitamin D supplementation in mitigating AD progression through its effects on pathological processes. However, challenges and controversies remain regarding the efficacy, optimal dosing regimens, and therapeutic strategies of vitamin D intake. Conclusion This review underscores the role of vitamin D as a modifiable factor and therapeutic target in Alzheimer’s disease. Further research is required to establish definitive dosing regimens and strategies to optimize the use of vitamin D in preventing and managing this debilitating neurological disorder.


Methionine restriction for cancer therapy: From preclinical studies to clinical trials
Nagaraju Bandaru, Shaik Mohammad Noor, Maha Lakshmi Kammili, Mohan Gandhi Bonthu, Alluri Pavani Gayatri, and Perli Kranti Kumar
Elsevier BV



Integrative Strategies for Ovarian Cancer Treatment: A Comprehensive Review from Ayush Perspectives
Alluri Pavani Gayatri, Bhaskara Raju Vatchavai, Nirmala Korukola, Naga Raju Bandaru, Srikanth Kumar Karumanchi, Afrin Sultana, and Mohan Gandi Bonthu
Informatics Publishing Limited
Background: Ovarian Cancer remains a crucial reason for mortality among gynaecological diseases despite advancements in surgical techniques and chemotherapy. Delayed diagnosis often contributes to the unfavourable prognosis of the disorder. Though the initial platinum-based doublet chemotherapy and successful surgical intervention are done, a considerable proportion of individuals ranging from 70 to 80 per cent experience disease recurrence. Aim: In this current review, the authors aim to explore the potentiality of Ayush (Ayurveda, Unani, Siddha, Yoga and Naturopathy and Homeopathy) approaching modern medication for treating ovarian cancer. Ayush encompasses various therapeutic modalities, including herbal remedies, diet and nutrition, yoga and meditation, and Panchakarma, which have been traditionally used or studied for their potential benefits in cancer management. Methods: We will discuss the fundamental aetiology, pathology, risk factors, and basic signs and symptoms of ovarian cancer, along with the available Ayush treatment options. Specifically, we will focus on Ayurvedic approaches, such as Rasayanas (Rejuvenated therapies) and Ayurveda Maintenance Therapy (AMT), which have shown promise in managing recurrent ovarian cancer. Results: Studies investigating the efficacy of Ayush interventions, including herbal and herbal-mineral formulations, in treating ovarian cancer reported sustainable outcomes. We will examine the documented Platinum-Free Interval (PFI) and Progression-Free Survival (PFS) following Ayush treatments to assess their potential impact on patient outcomes. Conclusion: This review provides a comprehensive overview of Ayush treatment options for ovarian cancer, aiming to improve the chances of survival for patients, particularly those diagnosed with advanced disease. Integrating Ayush approaches with modern medical interventions may provide a comprehensive strategy for the management of ovarian cancer, confronting both psychological and physical aspects of this disorder.


Targeting the Polyol Pathway with Pithecellobium dulce for Diabetic Cataract Prevention
Nagaraju Bandaru, Naga Venkata Indira Devi Jajula, Yaso Deepika Mamidisetti, Mohan Gandhi Bonthu, Alluri Pavani Gayatri, Makarand Suresh Gambhire, Perla Swathi, Dodda Thulase Nadhreddy, and Prasanna Kumari Tata
Informatics Publishing Limited
Background: Diabetic cataracts are a prevalent complication in diabetes, primarily resulting from the accumulation of sorbitol in the lens via the polyol pathway. Targeting the polyol pathway to prevent diabetic cataracts has garnered significant interest. Pithecellobium dulce, a traditional medicinal plant, has shown potential in inhibiting aldose reductase activity, thereby reducing sorbitol accumulation. Aim: The present study investigates the efficacy of P. dulce in preventing diabetic cataract formation by modulating the polyol pathway. Methods: In this study, goat lenses were used for anti-cataract activity. A total of 16 lenses were divided into 4 groups group I normal control, group II Dexamethasone, group III and IV methanolic extract of P. dulce 100mg/ml, 200mg/ml respectively. At the end of the study, goat lenses were examined for opalescence score and estimate the Lens polyol, biochemical parameters like sodium, potassium, total proteins and catalase levels. Results: Dexamethasone treated lens showed more opalescence and increased levels of polyols, electrolytes and catalase levels when compared to the normal group. Whereas methanolic extract of P. dulce 200mg/ml treated lens significantly decreased opalescence, decreased levels of polyols, electrolytes and catalase levels when compared to dexamethasone-treated groups. Conclusion: Targeting the polyol pathway with P. dulce holds significant promise for the prevention of diabetic cataracts. Through its inhibitory effects on the polyol pathway, P. dulce can potentially mitigate the excessive accumulation of sorbitol within the lens, thus preventing the emergence and progression of cataracts in diabetic individuals.


A Schematic Review on Prevalence of and Society’s Reaction to Child Sexual Abuse
Balakrishna Talamanchi, Mohan Gandhi Bonthu, Pavani Gayatri Alluri, and Avisa Indira
Springer Nature Singapore



An Improved approach for Creating and Validating a Bioanalytical Method to Quantify Baloxavir Marboxil in Human Plasma using LC-API-MS/MS
Mohan Gandhi Bonthu, G.Raveendra Babu, Bhaskara Raju Vatchavai, Nirmala Korukola, and Alluri Pavani Gayatri
A and V Publications
This study describes a new method for accurately measuring baloxavir marboxil (BXM) in human plasma using LC-API-MS/MS. We employed baloxavir marboxil d4 as an internal standard for precise quantification. BXM and the internal standard were isolated from plasma using tert-butyl methyl ether (TBME) extraction. The LC system separated BXM and the internal standard on a Zorbax SB C18column, and tandem mass spectrometry (MS/MS) detected them with minimal interference from human plasma components. The method showed excellent linearity (R²≥0.9956) across a wide concentration range (10.610–1229.080ng/mL). BXM stability was evaluated under various conditions, including room temperature, injector storage, freeze-thaw cycles, and long-term storage at -70°C. The method was validated according to USFDA guidelines, demonstrating its effectiveness for quantifying BXM in human plasma for pharmacokinetic studies.







Evaluation of Anti-inflammatory Activity of Millingtonia hortensis Leaf Extract




A Bibliometric Analysis of Investigations on Black Pepper Published from 1978 to 2023




Exploration of in vitro Antioxidant and Cytotoxic Activities of Whole Plant Extracts of Rhynchosia heynei Wight & Arn: An Endemic Medicinal Herbaceous Under Shrub of Eastern Ghats of India
Nirmala Korukola, Girija Sastry Vedula, Mohan Gandhi Bonthu, and Lakshmana Rao Atmakuri
Asian Journal of Chemistry
This study reports the antioxidant and anticancer potential of Rhynchosia heynei, a widely used plant in traditional medicine, against various cancerous cell lines. The study found that R. heynei exhibited significant radical scavenging activity, indicating its potential as a natural antioxidant compound to guard against oxidative stress-induced diseases, including cancer. The plant extracts also displayed selective cytotoxicity towards various cell lines, with the most potent cytotoxicity exhibited by the ethanol extract towards the MCF-7 cell line and diethyl ether extract towards the HCT-116 cell line. The hexane extract exhibited substantial cytotoxic activity against HepG2, HCT-116 and L6 cell lines. However, the aqueous extract of R. heynei was the least cytotoxic of all the cell lines employed. Overall, the cytotoxic activity of R. heynei extracts was majorly selective towards MCF-7 and HCT-116. The findings suggest that R. heynei could be a potent natural product for developing anticancer drugs with fewer side effects. Further, in vitro and in vivo studies are required to determine the exact mechanism of action of the plant’s potential anticancer activity.


Antioxidant and anti-inflammatory activity screening of lasia spinosa rhizome and its validation using a computational simulation approach
Arya Lakshmi Marisetti, Mohan Gandhi Bonthu, and Ganga Rao Battu
EManuscript Technologies
Background: The present work assesses the antioxidant and anti-inflammatory effect of Lasia spinosa rhizome extracts on in-vitro and in-vivo models compiled through molecular docking study of plant-steemed phytocompounds with specific targets. Materials and Methods: In this study, Lasia spinosa rhizome was subjected to extraction using petroleum ether, ethyl acetate and methanol and the extracts were analyzed by GC-MS. Antioxidant was assessed using in-vitro methods such as DPPH scavenging activity and H2O2 scavenging activity; anti-inflammatory activity was assessed using both in-vitro and in-vivo and molecular docking utilizing Auto dock 4.0 was done. Results: Tests showed that methanolic extract (MELS) has the most important dose-dependent antioxidant and anti-inflammatory efficacy at various levels. Of all compounds, Morin reported the most successful docking ranking of -8.2 to -9.8, maintaining a good binding fondness between protein and ligand. Conclusion: Antioxidant and anti-inflammatory of Lasia spinosa may be inferred from the examinations. The in-vitro, in-vivo and in silico assays of L. spinosa. Morin is confirmed by the information as a beneficial antioxidant and anti-inflammatory agent that can aid future clinical assessments.


Simultaneous determination of candesartan and hydrochlorothiazide in human plasma by LC-MS/MS
Mohan Gandhi Bonthu, Lakshmana Rao Atmakuri, and Venkateswara Rao Jangala
FapUNIFESP (SciELO)
A simple, sensitive, rapid and highly efficient LC-MS/MS method was developed for the determination of Candesartan and Hydrochlorothiazide simultaneously in human plasma. The method employed Zorbax eclipse C18 (150 X 4.6 mm, 5µ) column using acetate buffer: acetonitrile (25:75%, v/v) as the mobile phase. The mobile phase flow rate is 1 mL/min which was delivered into the mass spectrometer electron spray ionization chamber. The Liquid/liquid extraction procedure was used in the method for the extraction of analytes. The chromatograph was attached to a negative ion mode tandem mass spectrometer and the method was validated for all the parameters as per the guidelines of US-FDA. The ions were detected in multiple reaction monitoring mode and the transitions are m/z 439.00®309.10 and 295.80®268.80 for candesartan and hydrochlorothiazide respectively. Isotopic standards were used as internal standards for effective recovery of the analytes. The drugs were analyzed over a calibration range of 1.027-302.047 ng/mL for candesartan and 1.044-306.945 ng/mL for hydrochlorothiazide respectively with regression coefficient greater than 0.99. The mean extraction recoveries are 96.95±5.61 and 100.55±4.82 for candesartan and hydrochlorothiazide respectively. The precision and accuracy values for all the studies were within the range of ≤15% and 85-115%. The performed stability studies indicate that the developed method is stable in plasma for 15 h at room temperature (bench top); 52 h (in injector); for 112 days at -70 oC for long term stability; five successive freeze and thaw cycles. The developed method could be successfully employed for the determination of selected drugs in biological samples.


UPLC-MS/MS method for determination of sofosbuvir in human plasma
B.M. Gandhi, A.L. Rao, and J.V. Rao
Elsevier BV



Method development and validation of uv spectrophotometric and stability indicating rp-hplc methods for simultaneous estimation of moxifloxacin hydrochloride and ketorolac tromethamine in bulk and opthalmic dosage forms




A new reversed-phase high-performance liquid chromatography method development and validation for the simultaneous estimation of ketorolac tromethamine and tramadol hydrochloride in pharmaceutical dosage forms
Bharathi Devi Y, Sumanth Srinivas Kamatham, Bhaskara Raju V, Mohan Gandhi B, and Srinivas K
Innovare Academic Sciences Pvt Ltd
Objective: This study was embarked upon to develop a new, simple, rapid, validated reversed-phase high-performance liquid chromatography (HPLC)method for the estimation of ketorolac tromethamine (KET) and tramadol hydrochloride (TDL) in pharmaceutical dosage forms.Methods: The HPLC method was developed on Shishiedo C18 column (250 mm × 4.6 mm i.d, 5 μ) using methanol: 50 mM phosphate buffer (pH 6.0) in the ratio of 52:48 at 282 nm.Results: Retention time for the drugs was found to be 5.1 and 6.9 minutes for tramadol and ketorolac, respectively. The limit of detection for tramadoland ketorolac were found to be 1.0 and 0.1 μg/ml, limit of quantitation for tramadol and ketorolac were found to be 5.0 and 0.5 μg/ml, respectively. Linearity was established in the range of 20.0-30.0 μg/ml and 8.0-12.0 μg/ml for TDL and KET, respectively. The method was precise with % relative standard deviation <2 for both intra- and interday precision. The accuracy of the method was performed over three levels of concentration, and the recovery was in the range of 98-102%.Conclusion: From the found experimental data, it can be concluded that the developed method is accurate, precise, and selective and can be employedsuccessfully for the estimation of KET and TDL in Pharmaceutical dosage forms.Keywords: Reversed-phase high-performance liquid chromatography, Ketorolac tromethamine, Tramadol hydrochloride.


A new stability-indicating and validated RP-HPLC method for the estimation of Liraglutide in bulk and pharmaceutical dosage forms
Jhansi Naga Pedaprolu, Mohan Bonthu, Bhaskara Vatchavai, Srinivas Kamatham, Srinivas Kolli, and Andal Naga Kapuganti
Modestum Publishing Ltd
A stability-indicating RP-HPLC method was developed and validated for the estimation of Liraglutide in bulk and pharmaceutical dosage forms. Shiseido C18 (250 mm x 4.6 mm I.D., 5 μm particle size) column was used as stationary phase with mobile phase consisting methanol+acetonitrile (80:20): phosphate buffer (pH 3.0 adjusted with ortho phosphoric acid) in the ratio of 75:25, v/v. The flow rate was maintained at 1.2 ml/min and effluents were monitored at 245 nm. The retention time was found to be 2.837 minutes. The forced degradation studies were performed as per ICH guidelines under acidic, alkali, oxidative, thermal, photostability and neutral conditions. The drug peak was well resolved from the peaks of degraded products. From the degradation studies it is evident that the drug showed instability under acidic, alkali, oxidative, thermal, photostability and neutral conditions. The linearity of the method was observed in the concentration range of 10-60 μg/ml with the number of theoretical plates & tailing factor being 5550 & 1.17 respectively with a correlation coefficient of 0.999. The percentage assay of Liraglutide was found to be 99.66%. The method was validated for its accuracy, precision and system suitability. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the estimation of Liraglutide in bulk and pharmaceutical dosage forms.


Development and validation of stability indicating reverse phase high-performance liquid chromatography method for simultaneous estimation of atenolol, hydrochlorothiazide and losartan in bulk and pharmaceutical dosage form




Validated HPTLC and stability indicating RP-HPLC methods for the simultaneous estimation of Moxifloxacin and Dexamethasone in bulk and ophthalmic dosage form
B. Gandhi, A. Rao, and J. Rao
Oriental Scientific Publishing Company
Novel HPTLC and stability indicating RP-HPLC methods were developed for simultaneous estimation of Moxifloxacin (MOX) and Dexamethasone (DEX) in ophthalmic dosage form. For HPTLC method, the separation was carried out on HPTLC aluminum plates using acetonitrile:water:ammonia (8:1:0.5 V/V/V) as mobile phase and developed plates were read at 266 nm. The drugs were resolved satisfactorily with Rf values of 0.09±0.01 and 0.74±0.01 for MOX and DEX, respectively. The RPHPLC analysis is carried out on Shiseido C18 column (250 mm × 4.6 mm I.D., 5 µm), using 0.02M acetate buffer (pH is 4 adjusted with triethylamine) and acetonitrile in the ratio of 60:40 V/V with a flow rate of 1.2 m/min and the detection was carried out at 254 nm. The retention times were found to be 2.144±0.5 min and 4.732±0.5 min. for MOX and DEX respectively. Developed methods were validated as per ICH guidelines and were found to be within the limits.


Validated spectrophotometric and stability indicating RP-HPLC methods for the simultaneous estimation of gatifloxacin and dexamethasone in ophthalmic dosage form