CHUN HOE TAN

EDUCATION

2018–22: Universiti Malaya (UM), PhD in Biochemistry and Biology
2014–18: Universiti Malaya (UM), BSc in Biochemistry
2007–13: Catholic High School, Petaling Jaya (SPM and STPM)

RESEARCH INTERESTS

Natural products, in vitro drug discovery, biochemistry

Scopus Publications

Discovery of indoleninyl-pyrazolo[3,4-b]pyridines as potent chemotherapeutic agents against colorectal cancer cells
Nur Husnaini Basir, Abdul Qaiyum Ramle, Min Phin Ng, Chun Hoe Tan, Edward R.T. Tiekink, Kae Shin Sim, Wan Jefrey Basirun, and Melati Khairuddean
Elsevier BV








Chippiine, iboga, cleavamine, vobasine, and cytotoxic bisindole alkaloids from Tabernaemontana polyneura
Sin-Yee Tang, Min-Phin Ng, Chun-Hoe Tan, Kae-Shin Sim, Kuan-Hon Lim, Kien-Thai Yong, Yun-Yee Low, and Siew-Huah Lim
Elsevier BV



Clinical symptoms and diagnoses of COVID-19




Medicinal plants for SARS-CoV-2 infections




Traditional plant-based therapies for SARS-CoV-2 infections




The pathogenesis of coronavirus through the host




Polyneurines A−H, iboga alkaloids from Tabernaemontana polyneura
Sin-Yee Tang, Chun-Hoe Tan, Kae-Shin Sim, Kien-Thai Yong, Kuan-Hon Lim, Yun-Yee Low, and Siew-Huah Lim
Elsevier BV



A new cinnamaldehyde-rhodamine based dual chemosensor for Cu²⁺ and Fe³⁺ and its applicability in live cell imaging
Nur Amira Solehah Pungut, Chun Hoe Tan, Hazwani Mat Saad, Kae Shin Sim, Sheena Yin Xin Tiong, Chee Wei Ang, Chun Hau Gan, Kien Voon Kong, and Kong Wai Tan
Elsevier BV



A new lipophilic cationic rhodamine-based chemosensor for detection of Al(III)/Cu(II) and intracellular pH change and its application as a smartphone-assisted sensor in water sample analysis
Wei Chuen Chan, Min Phin Ng, Chun Hoe Tan, Chee Wei Ang, Kae Shin Sim, Sheena Yin Xin Tiong, Nur Amira Solehah Pungut, Chew Hee Ng, and Kong Wai Tan
Elsevier BV



Artocarpus heterophyllus Lam. Stem bark inhibits melanogenesis through regulation of ROS, cAMP, and MAPK pathways
Hazwani Mat Saad, Chun Hoe Tan, Sugumaran Manickam, Siew Huah Lim, and Kae Shin Sim
Penerbit Universiti Kebangsaan Malaysia (UKM Press)
Natural-based skin-lightening cosmeceutical products are attracting high popularity nowadays due to their relatively high bioavailability upon application. Artocarpus species have been highlighted with such potential, and our previous studies have reported that Artocarpus heterophyllusLam. stem bark extract exhibited a potent anti-melanogenic activity by reducing melanin content and inhibiting cellular tyrosinase activity in B16F10 melanoma cells. Hence, this study aimed to identify the bioactive fraction from A. heterophyllus Lam. stem bark and determine its anti-melanogenic mechanisms in B16F10 melanoma cells. Our results showed that a fraction (H-3) demonstrated the most pronounced anti-melanogenic effect at 12.00 µg/mL by reducing melanin content to 22.86 ± 2.90% and inhibiting cellular tyrosinase activity at treatment concentration 33-fold lower than kojic acid, without being cytotoxic against B16F10 melanoma cells. Moreover, treatment with H-3 for 24 and 48 h substantially scavenged intracellular reactive oxygen species (ROS) of hydrogen peroxide-challenged B16F10 melanoma cells by 1.8 and 4.4%, respectively. Based on the microarray profiling and qPCR analysis, H-3 downregulated Creb3l1, Creb3l2, Creb3l3, Mitf, Tyr, Tyrp1, and Dct genes in B16F10 melanoma cells, whereas the expression of Map3k20, Mapk14 (p38), and Foxo3 genes was markedly increased. Altogether, these results demonstrated that H-3 exhibited its anti-melanogenic activity in B16F10 melanoma cells through scavenging ROS and concurrent inhibition of the cAMP and activation of the p38/MAPK signaling pathways. These findings indicate that H-3 has the potential to be used as a skin lightening cosmeceutical agent in the treatment of skin hyperpigmentation.


Structural insights and cytotoxicity evaluation of benz[e]indole pyrazolyl-substituted amides
Abdul Qaiyum Ramle, Nadia Nabihah Mohd Yusof Chan, Min Phin Ng, Chun Hoe Tan, Kae Shin Sim, Edward R. T. Tiekink, and Chee Chin Fei
Springer Science and Business Media LLC



Antiproliferative and Microtubule-stabilizing Activities of Two Iboga-vobasine Bisindoles Alkaloids from Tabernaemontana corymbosa in Colorectal Adenocarcinoma HT-29 Cells
Chun Hoe Tan, Dawn Su Yin Sim, Siew Huah Lim, Taznim Begam Mohd Mohidin, Gokula Mohan, Yun Yee Low, Toh Seok Kam, and Kae Shin Sim
Georg Thieme Verlag KG
AbstractTwo iboga-vobasine bisindoles, 16′-decarbomethoxyvoacamine (1) and its 19,20-dihydro derivative, 16′-decarbomethoxydihydrovoacamine (2) from Tabernaemontana corymbosa exhibited potent cytotoxicity against the human colorectal adenocarcinoma HT-29 cells in our previous studies. Bisindoles 1 and 2 selectively inhibited the growth of HT-29 cells without significant cytotoxicity to normal human colon fibroblasts CCD-18Co. Treatment with bisindoles 1 and 2 suppressed the formation of HT-29 colonies via G0/G1 cell cycle arrest and induction of mitochondrial apoptosis. Owing to its higher antiproliferative activity, bisindole 2 was chosen for the subsequent studies. Bisindole 2 inhibited the formation of HT-29 spheroids (tumor-like cell aggregates) in 3D experiments in a dose-dependent manner, while an in vitro tubulin polymerization assay and molecular docking analysis showed that bisindole 2 is a microtubule-stabilizing agent which is predicted to bind at the β-tubulin subunit at the taxol-binding site. The binding resulted in the generation of ROS, which consequently activated the oxidative stress-related cell cycle arrest and apoptotic pathways, viz., JNK/p38, p21Cip1/Chk1, and p21Cip1/Rb/E2F, as shown by microarray profiling.


Synthesis, characterisation and biological activity of diorganotin compounds of (E)-N'-(5-nitro-2-hydroxybenzylidene)-3-hydroxy-2-naphthohydrazide
See Mun Lee, Kong Mun Lo, Li Yuan Liew, Jactty Chew, Chun Hoe Tan, Kae Shin Sim, and Edward R.T. Tiekink
Elsevier BV



Group 6 photo-activable carbon monoxide-releasing molecules (PhotoCORMs) with 1’10-phenanthroline based ligand as potential anti-proliferative and anti-microbial agents
Shiaw Xian Lee, Chun Hoe Tan, Wee Li Mah, Richard Chee Seng Wong, Ninie Suhana Abdul Manan, Yuen Lin Cheow, Kae Shin Sim, and Kong Wai Tan
Elsevier BV



Synthesis, anticancer and antimalarial activities of organosulfur and organoselenium derivatives of cyclopentadienyliron dicarbonyl as photoCORMs
Chun Hau Gan, Kong Wai Tan, Mei Lee Ooi, Jonathan Wee Kent Liew, Yee Ling Ng, Yee Ling Lau, Yin Zhuang Ng, Chew Hee Ng, Chun Hoe Tan, and Richard C.S. Wong
Elsevier BV



Synthesis of phenanthroline-based ligand and its UV activable tetracarbonyl photoCORMs based on chromium, molybdenum, and tungsten as cytotoxic and antimicrobial agents
Shiaw Xian Lee, Chun Hoe Tan, Wee Li Mah, Richard Chee Seng Wong, Kae Shin Sim, Yuen Lin Cheow, Chew-Hee Ng, and Kong Wai Tan
Elsevier BV



Nickel(II) Complexes with Polyhydroxybenzaldehyde and O,N,S tridentate Thiosemicarbazone ligands: Synthesis, Cytotoxicity, Antimalarial Activity, and Molecular Docking Studies.
Savina Savir, Jonathan Wee Kent Liew, Indra Vythilingam, Yvonne Ai Lian Lim, Chun Hoe Tan, Kae Shin Sim, Vannajan Sanghiran Lee, Mohd. Jamil Maah, and Kong Wai Tan
Elsevier BV



Synthesis of group 6 (chromium, molybdenum, and tungsten) photoCORMs as potential antimicrobial and anticancer agents
Shiaw Xian Lee, Chun Hoe Tan, Wee Li Mah, Richard Chee Seng Wong, Yuen Lin Cheow, Kae Shin Sim, and Kong Wai Tan
Elsevier BV



Evaluation of antioxidant activity and phytochemical composition of Baeckea frutescens and Leptospermum javanicum essential oils
Hazwani Mat Saad, Syarifah Nur Syed Abdul Rahman, Suerialoasan Navanesan, Chun Hoe Tan, Sugumaran Manickam, Sri Nurestri Abd Malek, and Kae Shin Sim
Elsevier BV



The Bisindole Alkaloids Angustilongines M and A from Alstonia penangiana Induce Mitochondrial Apoptosis and G0/G1 Cell Cycle Arrest in HT-29 Cells through Promotion of Tubulin Polymerization
Chun-Hoe Tan, Joanne Soon-Yee Yeap, Siew-Huah Lim, Yun-Yee Low, Kae-Shin Sim, and Toh-Seok Kam
American Chemical Society (ACS)
A new linearly fused macroline-sarpagine bisindole, angustilongine M (1), was isolated from the methanolic extract of Alstonia penangiana. The structure of the alkaloid was elucidated based on analysis of the spectroscopic data, and its biological activity was evaluated together with another previously reported macroline-akuammiline bisindole from the same plant, angustilongine A (2). Compounds 1 and 2 showed pronounced in vitro growth inhibitory activity against a wide panel of human cancer cell lines. In particular, the two compounds showed potent and selective antiproliferative activity against HT-29 cells, as well as strong growth inhibitory effects against HT-29 spheroids. Cell death mechanistic studies revealed that the compounds induced mitochondrial apoptosis and G0/G1 cell cycle arrest in HT-29 cells in a time-dependent manner, while in vitro tubulin polymerization assays and molecular docking analysis showed that the compounds are microtubule-stabilizing agents, which are predicted to bind at the β-tubulin subunit at the Taxol-binding site.


Evaluation of anti-melanogenesis and free radical scavenging activities of five Artocarpus species for cosmeceutical applications
Hazwani Mat Saad, Chun Hoe Tan, Siew Huah Lim, Sugumaran Manickam, and Kae Shin Sim
Elsevier BV



Arbolodinines A−C, biologically-active aspidofractinine-aspidofractinine, aspidofractinine-strychnan, and kopsine-strychnan bisindole alkaloids from Kopsia arborea
Soon-Kit Wong, Joanne Soon-Yee Yeap, Chun-Hoe Tan, Kae-Shin Sim, Siew-Huah Lim, Yun-Yee Low, and Toh-Seok Kam
Elsevier BV



Evaluation of DNA Binding and Topoisomerase I Inhibitory Activities of 16′-Decarbomethoxydihydrovoacamine from Tabernaemontana corymbosa
Chun Hoe Tan, Dawn Su Yin Sim, Mok Piew Heng, Siew Huah Lim, Yun Yee Low, Toh Seok Kam, and Kae Shin Sim
Wiley



Macroline, talpinine, and sarpagine alkaloids from Alstonia penangiana. An NMR-based method for differentiating between A. penangiana and A. macrophylla
Joanne Soon-Yee Yeap, Chun-Hoe Tan, Kien-Thai Yong, Kuan-Hon Lim, Siew-Huah Lim, Yun-Yee Low, and Toh-Seok Kam
Elsevier BV