CLAUDIA CORTIMIGLIA

Scopus Publications

Sr2+ hydroxyapatite and polylactic acid nanoparticles doped antimicrobial bioactive chitosan-based coating for medical device osteointegration
Amedeo Ungolo, Marco Ruggeri, Laura Caliogna, Claudia Cortimiglia, Daniele Callegari, et al.
International Journal of Biological Macromolecules, 2026
Cinnamon-enriched chocolate pralines as a delivery system for Lacticaseibacillus rhamnosus CRL1505: viability and digestive stability
Marianna Bozzetti, Eleonora Galli, Claudia Cortimiglia, Ernesto Carrega, Mariasole Cervini, et al.
Food Bioscience, 2026
Comprehensive genome-wide analysis for the safety assessment of microbial biostimulants in agricultural applications
Gabriele Bellotti, Claudia Cortimiglia, Maria Elena Antinori, Pier Sandro Cocconcelli, Edoardo Puglisi
Microbial Genomics, 2025
Microbial biostimulants (MBs) offer a sustainable approach to agriculture by helping to reduce reliance on synthetic fertilizers. However, as MBs are intentionally released into the environment, their safety should be rigorously assessed. While taxa with qualified presumption of safety (QPS) benefit from established safety indications, non-QPS taxa lack such guidance. To address this gap, we propose a pipeline combining whole genome sequencing (WGS) and extensive literature search (ELS) data to evaluate microbial safety. We analysed public genomes of three QPS species (Rhodopseudomonas palustris, Bacillus velezensis, Priestia megaterium) and four non-QPS genera (Arthrobacter, Azotobacter, Azospirillum, Herbaspirillum), screening them for virulence factors (VFs), antimicrobial resistance (AMR) genes and mobile genetic elements (MGEs). Results confirmed the safety of QPS taxa, revealing no VFs and only a few intrinsic and non-clinically relevant AMRs. Among non-QPS taxa, VF hits were more prevalent in Azotobacter and Azospirillum spp., though they were mostly related to beneficial plant interactions rather than pathogenicity. AMR genes in non-QPS taxa were primarily associated with efflux pumps or were sporadically distributed. Notably, the only genus-wide pattern observed was that most Azospirillum and Herbaspirillum genomes harboured chromosomally encoded β-lactamases sharing similar genetic structures; however, the detected β-lactamase (bla) genes were distantly related to clinically relevant bla variants, and the absence of MGEs suggests a low risk of horizontal gene transfer, indicating the overall safety of these genera. In general, this WGS–ELS framework provides a robust tool for assessing the safety of non-QPS MBs, supporting regulatory decision-making and ensuring their safe use in sustainable agriculture while safeguarding public health.
Tenebrio molitor Frass: A Cutting-Edge Biofertilizer for Sustainable Agriculture and Advanced Adsorbent Precursor for Environmental Remediation
Alessandra Verardi, Paola Sangiorgio, Brigida Della Mura, Stefania Moliterni, Anna Spagnoletta, et al.
Agronomy, 2025
The projected growth of the global population to over 10 billion by 2080 necessitates groundbreaking sustainable agricultural solutions that enhance productivity while mitigating environmental impacts. Tenebrio molitor frass (TMF), derived from larval excrement and exuviae, has emerged as a promising organic fertilizer. Enriched with macro- and micronutrients, TMF enhances soil functions through microbial communities that promote nutrient cycling, decompose organic matter, and suppress soilborne pathogens. Additionally, functional compounds like chitin, cellulose, xylans, and lignin improve the soil structure, foster beneficial microbes, and activate natural plant defence responses. The synergy of microbial activity and bioactive compounds positions TMF as a valuable resource for enhancing plant growth and soil health. Its role as a nutrient source, biostimulant, and soil amendment aligns with circular economy principles by recycling agro-industrial by-products and reducing reliance on synthetic fertilizers. TMF also contributes to sustainable agriculture by improving soil fertility, microbial biodiversity, and plant stress resilience, while mitigating greenhouse gas emissions and nutrient runoff. Additionally, TMF-derived biochar offers the potential for environmental remediation as an effective adsorbent. Despite its advantages, TMF faces challenges in scalability, cost, and regulations, requiring advancements in processing, enrichment, and supportive policies to maximize its potential in sustainable farming.
Antibiotic resistance and novel Sequence Types of Klebsiella spp. in human, animal, and food sources: a One Health perspective from Northern Nigeria
Ayodele Timilehin Adesoji, Vittoria Mattioni Marchetti, Claudia Cortimiglia, Francesca Piscopiello, Ilaria Petrizzi, et al.
Current Research in Microbial Sciences, 2025
Evaluating the Genome-Based Average Nucleotide Identity Calculation for Identification of Twelve Yeast Species
Claudia Cortimiglia, Javier Alonso-Del-Real, Mireya Viviana Belloso Daza, Amparo Querol, Giovanni Iacono, et al.
Journal of Fungi, 2024
Classifying a yeast strain into a recognized species is not always straightforward. Currently, the taxonomic delineation of yeast strains involves multiple approaches covering phenotypic characteristics and molecular methodologies, including genome-based analysis. The aim of this study was to evaluate the suitability of the Average Nucleotide Identity (ANI) calculation through FastANI, a tool created for bacterial species identification, for the assignment of strains to some yeast species. FastANI, the alignment of in silico-extracted D1/D2 sequences of LSU rRNA, and multiple alignments of orthologous genes (MAOG) were employed to analyze 644 assemblies from 12 yeast genera, encompassing various species, and on a dataset of hybrid Saccharomyces species. Overall, the analysis showed high consistency between results obtained with FastANI and MAOG, although, FastANI proved to be more discriminating than the other two methods applied to genomic sequences. In particular, FastANI was effective in distinguishing between strains belonging to different species, defining clear boundaries between them (cutoff: 94–96%). Our results show that FastANI is a reliable method for attributing a known yeast species to a particular strain. Moreover, although hybridization events make species discrimination more complex, it was revealed to be useful in the identification of these cases. We suggest its inclusion as a key component in a comprehensive approach to species delineation. Using this approach with a larger number of yeasts would validate it as a rapid technique to identify yeasts based on whole genome sequences.
Microplastic-Mediated Transfer of Tetracycline Resistance: Unveiling the Role of Mussels in Marine Ecosystems
Giovanni Milani, Claudia Cortimiglia, Mireya Viviana Belloso Daza, Emanuele Greco, Daniela Bassi, et al.
Antibiotics, 2024
The global threat of antimicrobial resistance (AMR) is exacerbated by the mobilization of antimicrobial resistance genes (ARGs) occurring in different environmental niches, including seawater. Marine environments serve as reservoirs for resistant bacteria and ARGs, further complicated by the ubiquity of microplastics (MPs). MPs can adsorb pollutants and promote bacterial biofilm formation, creating conditions favorable to the dissemination of ARGs. This study explores the dynamics of ARG transfer in the marine bivalve Mytilus galloprovincialis within a seawater model, focusing on the influence of polyethylene MPs on the mobilization of the Tn916-carrying tetM gene and plasmid-encoded ermB. Experiments revealed that biofilm formation on MPs by Enterococcus faecium and Listeria monocytogenes facilitated the transfer of the tetM resistance gene, but not the ermB gene. Furthermore, the presence of MPs significantly increased the conjugation frequency of tetM within mussels, indicating that MPs enhance the potential for ARG mobilization in marine environments. These findings highlight the role of MPs and marine organisms in ARG spread, underscoring the ecological and public health implications.
Development of Coated PLA Films Containing a Commercial Olive Leaf Extract for the Food Packaging Sector
Cecilia Fiorentini, Giulia Leni, Elena Díaz de Apodaca, Laura Fernández-de-Castro, Gabriele Rocchetti, et al.
Antioxidants, 2024
A commercial olive leaf extract (OL), effective against Salmonella enterica, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus, was added to three different coating formulations (methylcellulose, MC; chitosan, CT; and alginate, ALG) to produce active polylactic acid (PLA) coated films. Evaluation of these coated PLA films revealed significant inhibition of S. aureus growth, particularly with the MC and CT formulations exhibiting the highest inhibition rates (99.7%). The coated films were then tested for food contact compatibility with three food simulants (A: 10% ethanol; B: 3% acetic acid; D2: olive oil), selected to assess their suitability for pre-cut hams and ready-to-eat vegetables in relation to overall migration. However, coated films with active functions exhibited migration values in simulants A and B above legal limits, while promising results were obtained for simulant D2, highlighting the need to deeply investigate these coatings’ impact on a real food system. Untargeted metabolomics revealed that the type of coating influenced the selective release of certain phenolic classes based on the food simulant tested. The Oxitest analysis of simulant D2 demonstrated that the MC and ALG-coated PLA films slightly slowed down the oxidation of this food simulant, which is an edible vegetable oil.
Companilactobacillus alimentarius: An extensive characterization of strains isolated from spontaneous fermented sausages
Giulia Tabanelli, Federica Barbieri, Alberto Baños, Jose Manuel Garcia Madero, Mireya Viviana Belloso Daza, et al.
International Journal of Food Microbiology, 2024
Validation of digital PCR assay for the quantification of Mycobacterium avium subsp. paratuberculosis in bovine faeces according to the ISO 20395:2019
Simone Russo, Claudia Cortimiglia, Anita Filippi, Giorgia Palladini, Chiara Garbarino, et al.
Journal of Microbiological Methods, 2023
Genome engineering of Stx1-and Stx2-converting bacteriophages unveils the virulence of the dairy isolate Escherichia coli O174:H2 strain UC4224
Giovanni Milani, Mireya Viviana Belloso Daza, Claudia Cortimiglia, Daniela Bassi, Pier Sandro Cocconcelli
Frontiers in Microbiology, 2023
Isothermal inactivation of Mycobacterium avium subsp. paratuberculosis in curd simulating the stretching phase in pasta-filata cheese process
Filippo Barsi, Elena Dalzini, Simone Russo, Elena Cosciani-Cunico, Paola Monastero, et al.
Frontiers in Microbiology, 2022
Genomic Insights of Enterococcus faecium UC7251, a Multi-Drug Resistant Strain From Ready-to-Eat Food, Highlight the Risk of Antimicrobial Resistance in the Food Chain
Mireya Viviana Belloso Daza, Giovanni Milani, Claudia Cortimiglia, Ester Pietta, Daniela Bassi, et al.
Frontiers in Microbiology, 2022
Wild Boars as an Indicator of Environmental Spread of ESβL-Producing Escherichia coli
Alessandra Mercato, Claudia Cortimiglia, Aseel Abualsha’ar, Aurora Piazza, Federica Marchesini, et al.
Frontiers in Microbiology, 2022
Citrus peel extracts for industrial-scale production of bio-based active food packaging
Cecilia Fiorentini, Guillermo Duserm Garrido, Andrea Bassani, Claudia Cortimiglia, Marta Zaccone, et al.
Foods, 2022
Colorimetric point-of-care detection of Clostridium tyrobutyricum spores in milk samples
Paola Cecere, Francesca Gatto, Claudia Cortimiglia, Daniela Bassi, Franco Lucchini, et al.
Biosensors, 2021
Genomic Investigation of Virulence Potential in Shiga Toxin Escherichia coli (STEC) Strains From a Semi-Hard Raw Milk Cheese
Claudia Cortimiglia, Maria Francesca Borney, Daniela Bassi, Pier Sandro Cocconcelli
Frontiers in Microbiology, 2021
Genome-based studies indicate that the enterococcus faecium clade b strains belong to enterococcus lactis species and lack of the hospital infection associated markers
Mireya Viviana Belloso Daza, Claudia Cortimiglia, Daniela Bassi, Pier Sandro Cocconcelli
International Journal of Systematic and Evolutionary Microbiology, 2021
Staphylococcus aureus From Goats Are Genetically Heterogeneous and Distinct to Bovine Ones
Alicia Romanò, Alessandra Gazzola, Valentina Bianchini, Claudia Cortimiglia, Antonio M. Maisano, et al.
Frontiers in Veterinary Science, 2020
Draft genome sequence of lactobacillus helveticus Lh 23, isolated from natural whey starter
Gaia Bertani, Daniela Bassi, Claudia Cortimiglia, Monica Gatti, Pier Sandro Cocconcelli, et al.
Microbiology Resource Announcements, 2020
Survey on the CRISPR arrays in Lactobacillus helveticus genomes
E. Scaltriti, D. Carminati, C. Cortimiglia, R. Ramoni, K.I. Sørensen, et al.
Letters in Applied Microbiology, 2019
Involvement of tyrosinase-related protein 1 gene in the light brown plumage phenotype of Falco cherrug
Claudia Cortimiglia, Bianca Castiglioni, Flavia Pizzi, Alessandra Stella, Emanuele Capra
Animal Genetics, 2017
Development of a droplet digital polymerase chain reaction for rapid and simultaneous identification of common foodborne pathogens in soft cheese
Paola Cremonesi, Claudia Cortimiglia, Claudia Picozzi, Giulietta Minozzi, Michela Malvisi, et al.
Frontiers in Microbiology, 2016
Short report prevalence of staphylococcus aureus and of methicillin-resistant s. Aureus clonal complexes in bulk tank milk from dairy cattle herds in lombardy region (Northern Italy)
C. CORTIMIGLIA, M. LUINI, V. BIANCHINI, L. MARZAGALLI, F. VEZZOLI, et al.
Epidemiology and Infection, 2016
Short communication: Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus in bulk tank milk from dairy goat farms in Northern Italy
C. Cortimiglia, V. Bianchini, A. Franco, A. Caprioli, A. Battisti, et al.
Journal of Dairy Science, 2015
Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank
E. Capra, P. Cremonesi, C. Cortimiglia, G. Bignoli, M. Ricchi, et al.
Letters in Applied Microbiology, 2014
The axonal guidance factor netrin-1 as a potential modulator of swine follicular function
G. Basini, C. Cortimiglia, L. Baioni, S. Bussolati, S. Grolli, et al.
Molecular and Cellular Endocrinology, 2011
Netrin-1: Just an axon-guidance factor?
L. Baioni, G. Basini, S. Bussolati, C. Cortimiglia, F. Grasselli
Veterinary Research Communications, 2010

CLAUDIA CORTIMIGLIA

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Scopus Publications