Clare Elizabeth O'Lone
@ecu.edu.au
Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science (CIPPS), School of Science
ARC Centre of Excellence for Innovations in Peptide and Protein Science (CIPPS), Edith Cowan University
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, Plant Science, Biochemistry, Genetics and Molecular Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Clare E. O'Lone, Angéla Juhász, Mitchell Nye-Wood, Hugh Dunn, David Moody, Jean-Philippe Ral, and Michelle L. Colgrave
Frontiers Media SA
Barley (Hordeum vulgare L.) is used in malt production for brewing applications. Barley malting involves a process of controlled germination that modifies the grain by activating enzymes to solubilize starch and proteins for brewing. Initially, the grain is submerged in water to raise grain moisture, requiring large volumes of water. Achieving grain modification at reduced moisture levels can contribute to the sustainability of malting practices. This study combined proteomics, bioinformatics, and biochemical phenotypic analysis of two malting barley genotypes with observed differences in water uptake and modification efficiency. We sought to reveal the molecular mechanisms at play during controlled germination and explore the roles of protein groups at 24 h intervals across the first 72 h. Overall, 3,485 protein groups were identified with 793 significant differentially abundant (DAP) within and between genotypes, involved in various biological processes, including protein synthesis, carbohydrate metabolism, and hydrolysis. Functional integration into metabolic pathways, such as glycolysis, pyruvate, starch and sucrose metabolism, revealed a metabolic rerouting due to low oxygen enforced by submergence during controlled germination. This SWATH-MS study provides a comprehensive proteome reference, delivering new insights into the molecular mechanisms underlying the impacts of low oxygen during controlled germination. It is concluded that continued efficient modification of malting barley subjected to submergence is largely due to the capacity to reroute energy to maintain vital processes, particularly protein synthesis.
Mahya Bahmani, Clare E. O’Lone, Angéla Juhász, Mitchell Nye-Wood, Hugh Dunn, Ian B. Edwards, and Michelle L. Colgrave
American Chemical Society (ACS)
Barley (Hordeum vulgare) is the fourth most cultivated crop in the world in terms of production volume, and it is also the most important raw material of the malting and brewing industries. Barley belongs to the grass (Poaceae) family and plays an important role in food security and food safety for both humans and livestock. With the global population set to reach 9.7 billion by 2050, but with less available and/or suitable land for agriculture, the use of biotechnology tools in breeding programs are of considerable importance in the quest to meet the growing food gap. Proteomics as a member of the “omics” technologies has become popular for the investigation of proteins in cereal crops and particularly barley and its related products such as malt and beer. This technology has been applied to study how proteins in barley respond to adverse environmental conditions including abiotic and/or biotic stresses, how they are impacted during food processing including malting and brewing, and the presence of proteins implicated in celiac disease. Moreover, proteomics can be used in the future to inform breeding programs that aim to enhance the nutritional value and broaden the application of this crop in new food and beverage products. Mass spectrometry analysis is a valuable tool that, along with genomics and transcriptomics, can inform plant breeding strategies that aim to produce superior barley varieties. In this review, recent studies employing both qualitative and quantitative mass spectrometry approaches are explored with a focus on their application in cultivation, manufacturing, processing, quality, and the safety of barley and its related products.
Susan J. Barker, Ping Si, Leon Hodgson, Margo Ferguson-Hunt, Yuphin Khentry, Priya Krishnamurthy, Susana Averis, Katharina Mebus, Claire O’Lone, Dumindi Dalugoda,et al.
Springer Science and Business Media LLC
Matthew N. Nelson, Huyen T. T. Phan, Simon R. Ellwood, Paula M. Moolhuijzen, James Hane, Angela Williams, Clare E. O‘Lone, John Fosu-Nyarko, Marie Scobie, Mehmet Cakir,et al.
Springer Science and Business Media LLC