@iniav.pt
Post Doctoral Researcher, – Phytobacteriology Laboratory, SAFSV
INIAV
PhD in Microbiology/Immunology (Aug 2014 – May 2020)
Thesis title: Studies on the immunological mechanisms leading to protection or pathology during malaria infection (
School of Biological Sciences, Nanyang Technological University & Laboratory of Pathogen Immunobiology, Singapore Immunology Network (SIgN), A*STAR, Singapore (Singapore)
MSc in Applied Microbiology (Oct 2008 – Nov 2010)
Thesis title: Selection and characterization of yeast starters from autochthonous populations in must.
Faculty of Sciences, University of Lisbon, Lisbon (Portugal)
BSc in Molecular Cell Biology and Genetics (Set 2004 – Set 2008)
Agricultural and Biological Sciences, Applied Microbiology and Biotechnology, Plant Science, Biochemistry, Genetics and Molecular Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Daniel McGuire, Telma Costa, Ana Tenreiro, Joana Cruz, Rui Sousa, Miguel Leão de Sousa, Carmo Martins, Francisco Pinto, Margarida Gama-Carvalho, Rogério Tenreiro,et al.
Springer Science and Business Media LLC
AbstractThe persistence and progression of Erwinia amylovora in naturally infected orchards is still not fully understood and the majority of studies have been carried out under controlled or artificial infections. A major hurdle stems from the inability to characterize the viability of the bacterium in its natural state. Most standard tests, including real-time qPCR, fail to differentiate live from dead cells, while culture dependent methods lack sensitivity. Even in orchards with recurring infections, the detection of E. amylovora is erratic. Immuno-flow cytometry (IFCM) is considered a high throughput tool in clinics and food industry but has been rarely used as diagnostic test for plant pathogens. Two pear orchards with fire blight history, located in the central part of Portugal, were selected for epidemiological monitoring between February and June, from 2019 to 2022. A total of 440 samples were tested using EPPO standard diagnostic protocols. To address the epidemiological behaviour of E. amylovora, we devised an IFCM test using specific monoclonal antibodies and viability dyes, validated in a subset of 149 samples, testing positive in routine diagnostic tests. Our IFCM test was able to differentiate live and dead E. amylovora in naturally infected samples with concentrations as low as 3.54 × 104 cell·mL−1, confirming positive results for 91% these samples, while 19.4% were considered positive by isolation, 38% using conventional PCR and 95% for real-time qPCR. Temporal analysis showed the highest number of positive samples regardless of the method employed in April, May and June, while viability values measured with IFCM were the highest in April, coinciding with the exit of dormancy and early flowering of pear trees. Integrative analysis of E. amylovora viability and population dynamics over the growing season allowed identification of distinct outbreak risks associated with these Portuguese pear orchards.
Yun Shan Goh, Daniel McGuire, and Laurent Rénia
Frontiers Media SA
Despite continuous efforts, the century-old goal of eradicating malaria still remains. Multiple control interventions need to be in place simultaneously to achieve this goal. In addition to effective control measures, drug therapies and insecticides, vaccines are critical to reduce mortality and morbidity. Hence, there are numerous studies investigating various malaria vaccine candidates. Most of the malaria vaccine candidates are subunit vaccines. However, they have shown limited efficacy in Phase II and III studies. To date, only whole parasite formulations have been shown to induce sterile immunity in human. In this article, we review and discuss the recent developments in vaccination with sporozoites and the mechanisms of protection involved.
P. L. Martínez-Morales, A. Revilla, I. Ocaña, C. González, P. Sainz, D. McGuire, and I. Liste
Springer Science and Business Media LLC