NURDIANAH HARIF FADZILAH
@usm.my
Scopus Publications
- Advances in paclitaxel nanoformulations: A systematic review of in vivo therapeutic efficacy and safety enhancements
Salma Seyam, Batoul Alallam, Nurdianah Harif Fadzilah, Erazuliana Abd Kadir
Journal of Controlled Release, 2025 - Therapeutic Evaluation of Ethanolic Bee Pollen Extract from Malaysian Stingless Bee in MCF-7 and MCF-10A Cell Lines
Nurdianah Harif Fadzilah, Wan Adnan Wan Omar
Pertanika Journal of Tropical Agricultural Science, 2023
Bee pollen is a complete food containing comprehensive nutrients and therapeutic properties that could eliminate free radicals. Three stingless bee species native to Malaysia were used to prepare bee pollen ethanolic extracts (BPE): Geniotrigona thoracica, Heterotrigona itama, and Tetrigona apicalis. The methodology used in this study was a trypan blue exclusion assay for cell proliferation activity in cultured breast adenocarcinoma human cell lines (MCF-7) and mammary epithelial human cell lines (MCF-10A). In addition, the therapeutic index (TI) was analyzed to assess the relative antiproliferative activity of BPE on cancer versus normal cells. Geniotrigona thoracica, BPE exhibited the highest therapeutic index (TI = 3.12) compared to H. itama (TI = 1.16) and T. apicalis (TI = 0.90) BPE. Each species represents different bioactive compounds due to different pollen foraging activities. Therefore, the highest TI species (G. thoracica) could be a potential candidate to be developed as a potential chemotherapeutic agent. - Long-term Survivorship of Breast Cancer Patients and Its Prognostic Factors: A Tertiary Oncological Center Experience in Penang
Malaysian Journal of Medicine and Health Sciences, 2019 - Gc-ms analysis of chemical constituents in ethanolic bee pollen extracts from three species of malaysian stingless bee
Wan Adnan Wan Omar, Noorfatimah Yahaya, Zainab Abdul Ghaffar, Nurdianah Harif Fadzilah
Journal of Apicultural Science, 2018
The pollen of stingless bees is derived from flower pollen mixed with bee digestive enzymes and preserved with honey and nectar. In this study, the volatile compounds present in ethanolic bee pollen extracts (BPEs) from three species of the Malaysian stingless bee were analyzed using gas chromatography-mass spectrometry (GC-MS). Hydrocarbons, sugars and its derivatives, fatty acids, amino acids, alcohol, uridine, aldehyde and an unknown carbamate were detected. Mannitol, the main sugar compounds, represented 54.34% in Trigona thoracica, 39.11% in Trigona apicalis and 33.05% in Trigona itama. Propanoic acid and hexadecanoic acid were the main hydrocarbons present in the extract of Trigona apicalis (4.04%) and Trigona thoracica pollen (1.28%) respectively. The polyunsaturated fatty acids linoleic acid and α-linolenic acid were found in small amounts in all BPEs (0.07-1.11%). The chemical compounds found in BPEs had biological activities, thus bee pollen may be useful in traditional medicine and as a health supplement. - Total phenolic content, total flavonoid and antioxidant activity of ethanolic bee pollen extracts from three species of Malaysian stingless bee
Nurdianah Harif Fadzilah, Mohd Fahimee Jaapar, Rosliza Jajuli, Wan Adnan Wan Omar
Journal of Apicultural Research, 2017
Bee pollen consists of flower pollen mixed with bee digestive enzymes and preserved with some honey and nectar. It contains high antioxidant activity due to the presence of polyphenols and flavonoids. This study was aimed at investigating the chemical profiles of bee pollen extracts from three species of Malaysian stingless bee, Trigona (Tetrigona) apicalis, Trigona (Heterotrigona) itama and Trigona (Geniotrigona) thoracica. Chemical profiles analyzed were total phenolic content (TPC), total flavonoid content (TFC) and antioxidant assay. T. apicalis showed the highest TPC value (135.93 ± 0.02 mg GAE/g) followed by T. thoracica (103.62 ± 0.04 mg GAE/g) and T. itama (33.46 ± 0.02 mg GAE/g). The concentration of flavonoids in this study showed that T. thoracica had the highest TFC (31.80 ± 0.13 mg QE/g), followed by T. apicalis (25.72 ± 0.17 mg QE/g) and T. itama (15.28 ± 0.04 mg QE/g). In antioxidant measurement, T. thoracica gave higher percentage of DPPH inhibition with the EC50 was 0.86 ± 0.01 mg/ml. This was followed by T. apicalis at concentration of 1.05 ± 0.01 mg/ml and T. itama at 3.24 ± 0.03 mg/ml. In conclusion, bee pollen extract from T. thoracica showed the highest TFC and DPPH inhibition whereas T. apicalis showed the highest TPC value. Due to the diversity and complexity of the bee pollen, further investigations should be performed to further evaluate the chemical profiles of polyphenolic composition, which may be important for its health promoting effects. - Interrelationship of clinicopathological features of breast cancer among different ethnic groups
Nizuwan A, Nurdianah HF, Musa MY
Jurnal Teknologi, 2016
Breast cancer is a heterogeneous disease that can be classified using a variety of clinical and pathological features. In Malaysia, breast cancer remains the most prevalence malignancy among women which vary substantially by racial/ ethnic group. A pilot study was conducted to determine clinical characteristics of breast cancer (BrCa) and the association with demographic factors from a set of data retrieved from Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (n = 236). In this study, 55.1% of the patients were Malay followed by Chinese (33.9%) and Indian (11.0%). By using Pearson Chi-Square test, it was reported that ethnicity was significantly associated with age at diagnosis (p = 0.001) and family history (p = 0.034). In addition, interrelationships (p value < 0.05) existed between: (1) ethnics with age at diagnosis (2) ER with PR, TNM staging and grading (3) PR with tumor grading. In summary, interrelationships status of BrCa clinicopathological features can be used to better understand BrCa in predicting prognosis for the patients. - Antioxidant activity of bee pollen ethanolic extracts from Malaysian stingless bee measured using DPPH-HPLC assay
International Food Research Journal, 2016 - Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines
Wan Adnan Wan Omar, Nur Asna Azhar, Nurdianah Harif Fadzilah, Nik Nur Syazni Nik Mohamed Kamal
Asian Pacific Journal of Tropical Biomedicine, 2016
To evaluate the antioxidant and antiproliferative effect of methanolic bee pollen extract (BPE) of Malaysian stingless bee [Lepidotrigona terminata (L. terminata)] and its synergistic effect with cisplatin (a chemotherapeutic drug) on MCF-7 cancer cell line. The antioxidant activity of BPE from L. terminata was measured by using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay. Antiproliferative activity at different concentrations of BPE and cisplatin was determined through using MTT assay on MCF-7 and L929 cell lines. An interaction effect (synergistic, additive and antagonistic) between BPE and cisplatin was determined by CompuSyn software based on MTT assay data. The EC50 (50% decrement of DPPH inhibition) of BPE was 0.5 mg/mL. L. terminata BPE exhibited antiproliferative activity on both cancer and normal cell lines. The IC50 (concentration of drug that was required for 50% of cell inhibition in vitro) of BPE on MCF-7 was 15 mg/mL whereas in normal cell line L929 was 26 mg/mL. The IC50 for cisplatin on MCF-7 was 20 μmol/L. The combination effect of BPE and cisplatin on MCF-7 cells showed that BPE at 15 mg/mL was able to potentiate the inhibitory effect of cisplatin at all different concentrations (2.5–20.0 mg/mL). The average of cancer cells inhibition which was potentiated by BPE was around 50%. A combination index values of less than 1 reported in the CompuSyn software further proved the synergistic effect between BPE and cisplatin, suggesting that BPE was working synergistically with cisplatin. Our study therefore suggested that BPE of Malaysian stingless bee, L. terminata is a potential chemopreventive agent and can be used as a supplementary treatment for chemotherapy drugs. BPE might be able to be used to potentiate the effect of chemotherapy drugs with the possibility to reduce the required dose of the drugs. The molecular mechanisms of how the BPE exerts antiproliferative activity will be a much interesting area to look for in future studies. - Seropositive status of dengue virus infection among blood donors in North Malaysia
NarazahM Yusoff, NurdianahF Harif, ZainoodinS. A Kader, SanmukhR Joshi
Asian Journal of Transfusion Science, 2014
Dengue virus (DENV) infection is a tropical disease causing morbidity and mortality. It is endemic in certain regions of Malaysia causing major public health problems. [1] It is transmitted largely through vector mosquito Aedes aegypti. The viremia among blood donors was reported from the endemic area of South American region. [2] In order to assess the magnitude of viremia among our blood donors, we screened serum samples from 360 random donors selected in a consecutive manner from blood donations during an outbreak of the disease from December 2009 to January 2010. Sample size was determined using online software sample size calculator for prevalence studies. [3] Commercial kits (PanBio, Australia) were used for detection of anti-DENV.
RECENT SCHOLAR PUBLICATIONS
- CELL CULTURE TECHNIQUES: A STANDARD OPERATING PROCEDURE
E Abd Kadir, AQN Hisham, NH Fadzilah
2025 - Advances in paclitaxel nanoformulations: A systematic review of in vivo therapeutic efficacy and safety enhancements
S Seyam, B Alallam, NH Fadzilah, E Abd Kadir
Journal of Controlled Release, 114036 , 2025
2025
Citations: 9 - Therapeutic evaluation of Ethanolic bee pollen extract from Malaysian stingless bee in MCF-7 and MCF-10A cell lines
NH Fadzilah, WA Wan Omar, WAW Omar
Pertanika J Trop Agric Sci 46 (1), 37-48 , 2023
2023
Citations: 8 - Minyak Kelapa Dara: Manfaat atau Mudarat?
NH Fadzilah
AMDI Newsletter 4 (1) , 2023
2023 - MyIPO Copyright: Projek Penyelidikan Libatsama Universiti–Komuniti: Ringkasan Tutorial Selendang Dua Muka
IN Zainon R, Harif Fadzilah N, Azman N, Md Toha Z
MY Patent App. LY2023P00,650 , 2023
2023 - MyIPO Copyright: Projek Penyelidikan Libatsama Universiti–Komuniti: Teknik Jahitan Baju Kurung Tradisional
IN Zainon R, Harif Fadzilah N, Azman N, Md Toha Z
MY Patent App. LY2023P00,649 , 2023
2023 - MyIPO Copyright: Projek Penyelidikan Libatsama Universiti–Komuniti: Teknik Jahitan Baju Kurung Moden
IN Zainon R, Harif Fadzilah N, Azman N, Md Toha Z
MY Patent App. LY2023P00,648 , 2023
2023 - MyIPO Copyright. Projek Penyelidikan Libatsama Universiti–Komuniti: Teknik Membuat Pola dan Jahitan Kurta Kanak-Kanak Lelaki
IN Zainon R, Harif Fadzilah N, Azman N, Md Toha Z
MY Patent App. LY2023P00,647 , 2023
2023 - Stingless Bee Pollen Extract Ameliorates DNA Damage and Increases DNA Methylation of Alu and LINE-1 Repetitive Elements
H Fadzilah N
AMDI Research Club 3/2023 , 2023
2023 - Therapeutic properties of Malaysian stingless bee pollen and its protective effect against DNA damage
OWA Harif Fadzilah N
The 6th International Conference on Molecular Diagnostics & Biomarker … , 2022
2022 - Antioxidant Activity and Cell Proliferation Assay of Malaysian Stingless Bee Pollen
OWA Harif Fadzilah N
1st Research Officers National Symposium (ReONS 2022) , 2022
2022 - The Effect of Ethanolic Bee Pollen Extract from Geniotrigona Thoracica on DNA Damage and DNA Methylation in HT-29 Colorectal Cell Line
NH Fadzilah
PhD, Universiti Sains Malaysia (USM) , 2022
2022 - Conference Proceedings–6th International Conference on Molecular Diagnostics and Biomarker Discovery (MDBD 2022): Building Resilience in Biomedical Research
WAWO Nurdianah Harif Fadzilah
BMC Proceedings 16 (7), O-16 , 2022
2022
Citations: 3 - Evaluation of Postgraduate Students Learning Experience at Universiti Sains Malaysia
ZR Harif Fadzilah N, Azman N, Zulkifle N
1st Asian International Mobility for Students (AIMS) International … , 2021
2021 - In vitro cytotoxicity activity of Acanthus ilicifolius on MCF-7 and MCF-7/TAMR-1 breast cancer cell lines
RSRA Harif Fadzilah N, Mohamad NM, Muhamad M, Zulkifli NI, Kamal NNSNM
International Conference of Integrative Medicine (eICIM) , 2021
2021 - Conference Proceedings–1st International Virtual Conference of Integrative Medicine 2021 (ICIM 2021)
RSRA Harif Fadzilah N, Mohamad NM, Muhamad M, Zulkifli NI, Kamal NNSNM
Journal of Complementary and Integrative Medicine 18 (4), S1–S57 , 2021
2021 - Genetik vs Epigenetik
OWA Harif Fadzilah N
Buletin Sejahtera , 2020
2020 - Long-term Survivorship of Breast Cancer Patients and Its Prognostic Factors: A Tertiary Oncological Center Experience in Penang.
N Azman, R Hami, NMM Mujar, NH Fadzillah, H Hussin, SI Shaukat, ...
Malaysian Journal of Medicine & Health Sciences 15 , 2019
2019
Citations: 3 - Pertahanan Antioksida Terhadap Kerosakan DNA
OWA Harif Fadzilah N
Buletin Sejahtera: Bioteknologi Pemangkin Inovasi Kesihatan 10, 117-120 , 2019
2019 - GC-MS analysis of chemical constituents in ethanolic bee pollen extracts from three species of Malaysian stingless bee
WAW Omar, N Yahaya, ZA Ghaffar, NH Fadzilah
Journal of Apicultural Science 62 (2), 275-284 , 2018
2018
Citations: 33
MOST CITED SCHOLAR PUBLICATIONS
- Bee pollen extract of Malaysian stingless bee enhances the effect of cisplatin on breast cancer cell lines
WAW Omar, NA Azhar, NH Fadzilah, NNSNM Kamal
Asian Pacific Journal of Tropical Biomedicine 6 (3), 265-269 , 2016
2016
Citations: 84 - Total phenolic content, total flavonoid and antioxidant activity of ethanolic bee pollen extracts from three species of Malaysian stingless bee
N Harif Fadzilah, MF Jaapar, R Jajuli, WA Wan Omar
Journal of Apicultural Research 56 (2), 130-135 , 2017
2017
Citations: 73 - Antioxidant activity of bee pollen ethanolic extracts from Malaysian stingless bee measured using DPPH-HPLC assay
HF Nurdianah, AHA Firdaus, OE Azam, WOW Adnan
International Food Research Journal 23 (1), 403 , 2016
2016
Citations: 35 - GC-MS analysis of chemical constituents in ethanolic bee pollen extracts from three species of Malaysian stingless bee
WAW Omar, N Yahaya, ZA Ghaffar, NH Fadzilah
Journal of Apicultural Science 62 (2), 275-284 , 2018
2018
Citations: 33 - Seropositive status of dengue virus infection among blood donors in North Malaysia
NF Harif, ZSA Kader, SR Joshi, NM Yusoff
Asian Journal of Transfusion Science 8 (1), 64 , 2014
2014
Citations: 18 - Advances in paclitaxel nanoformulations: A systematic review of in vivo therapeutic efficacy and safety enhancements
S Seyam, B Alallam, NH Fadzilah, E Abd Kadir
Journal of Controlled Release, 114036 , 2025
2025
Citations: 9 - Therapeutic evaluation of Ethanolic bee pollen extract from Malaysian stingless bee in MCF-7 and MCF-10A cell lines
NH Fadzilah, WA Wan Omar, WAW Omar
Pertanika J Trop Agric Sci 46 (1), 37-48 , 2023
2023
Citations: 8 - Contenido total fenólico y flavonoide, y actividad antioxidante en extractos etanólicos de polen de tres especies diferentes de abeja malasia sin aguijón
N Harif Fadzilah, MF Jaapar, R Jajuli, WA Wan Omar
J. Apic. Res 56, 130-135 , 2017
2017
Citations: 7 - Conference Proceedings–6th International Conference on Molecular Diagnostics and Biomarker Discovery (MDBD 2022): Building Resilience in Biomedical Research
WAWO Nurdianah Harif Fadzilah
BMC Proceedings 16 (7), O-16 , 2022
2022
Citations: 3 - Long-term Survivorship of Breast Cancer Patients and Its Prognostic Factors: A Tertiary Oncological Center Experience in Penang.
N Azman, R Hami, NMM Mujar, NH Fadzillah, H Hussin, SI Shaukat, ...
Malaysian Journal of Medicine & Health Sciences 15 , 2019
2019
Citations: 3 - Interrelationship of Clinicopathological Features of Breast Cancer Among Different Ethnic Groups
MY Musa
Jurnal Teknologi , 2016
2016
Citations: 1 - CELL CULTURE TECHNIQUES: A STANDARD OPERATING PROCEDURE
E Abd Kadir, AQN Hisham, NH Fadzilah
2025 - Minyak Kelapa Dara: Manfaat atau Mudarat?
NH Fadzilah
AMDI Newsletter 4 (1) , 2023
2023 - MyIPO Copyright: Projek Penyelidikan Libatsama Universiti–Komuniti: Ringkasan Tutorial Selendang Dua Muka
IN Zainon R, Harif Fadzilah N, Azman N, Md Toha Z
MY Patent App. LY2023P00,650 , 2023
2023 - MyIPO Copyright: Projek Penyelidikan Libatsama Universiti–Komuniti: Teknik Jahitan Baju Kurung Tradisional
IN Zainon R, Harif Fadzilah N, Azman N, Md Toha Z
MY Patent App. LY2023P00,649 , 2023
2023 - MyIPO Copyright: Projek Penyelidikan Libatsama Universiti–Komuniti: Teknik Jahitan Baju Kurung Moden
IN Zainon R, Harif Fadzilah N, Azman N, Md Toha Z
MY Patent App. LY2023P00,648 , 2023
2023 - MyIPO Copyright. Projek Penyelidikan Libatsama Universiti–Komuniti: Teknik Membuat Pola dan Jahitan Kurta Kanak-Kanak Lelaki
IN Zainon R, Harif Fadzilah N, Azman N, Md Toha Z
MY Patent App. LY2023P00,647 , 2023
2023 - Stingless Bee Pollen Extract Ameliorates DNA Damage and Increases DNA Methylation of Alu and LINE-1 Repetitive Elements
H Fadzilah N
AMDI Research Club 3/2023 , 2023
2023 - Therapeutic properties of Malaysian stingless bee pollen and its protective effect against DNA damage
OWA Harif Fadzilah N
The 6th International Conference on Molecular Diagnostics & Biomarker … , 2022
2022 - Antioxidant Activity and Cell Proliferation Assay of Malaysian Stingless Bee Pollen
OWA Harif Fadzilah N
1st Research Officers National Symposium (ReONS 2022) , 2022
2022
