DR DINESH GIRI

@kunainital.ac.in

ASSISTANT PROFESSOR DEPARTMENT OF BOTANY GOVT PG COLLEGE GOPESHWAR CHAMOLI UTTARAKHNAD
kumaun university nainital

DR DINESH GIRI


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https://researchid.co/dineshgiri

EDUCATION

MPhil, PhD IN BOTANY

RESEARCH, TEACHING, or OTHER INTERESTS

Plant Science
5

Scopus Publications

Scopus Publications

  • Induction of somatic embryogenesis in vulnerable medicinal plant Hedychium spicatum Buch-Ham ex Smith
    Dinesh Giri, Sushma Tamta
    Plant Tissue Culture and Biotechnology, 2013
    This protocol has been developed for somatic embryogenesis in Hedychium spicatum. Simultaneously, a method has also been developed for the production of synthetic seeds by using somatic embryos. Direct somatic embryos were developed on cotyledon explants of zygotic embryos on MS supplemented with high concentration of NAA (20.0 µM). Induction of secondary embryogenesis was best in 2,4-D supplemented medium fortified with activated charcoal. Germination of somatic embryos was enhanced by using GA3. Besides this, round and semi-hard beads of somatic embryos (synthetic seeds) could be produced by using 2% Na-alginate and 100 mM calcium chloride and more than 30% germination of synthetic seeds was achieved in MS. Well acclimated plants produced via somatic embryogenesis and/or synthetic seeds were transferred to field where more than 60% survived. This simple study enabled us to obtain a number of plantlets throughout the year each cycle requiring a short period of time. Besides propagation, this study provided an ex situ method for conservation of this vulnerable Himalayan species.D. O. I.http://dx.doi.org/10.3329/ptcb.v23i2.17506Plant Tissue Cult. & Biotech. 23(2): 147-155, 2013 (December)
  • Effects of plant growth substances on rooting of Hedychium Spicatum under different temperature regimes
    Dinesh Giri, Sushma Tamta
    Pakistan Journal of Biological Sciences, 2013
    Present study was carried out to develop a simple and efficient vegetative propagation protocol by applying various treatments to rhizome cuttings with different test solutions of auxins and phenolic compound. These were alpha-naphthalene acetic acid (NAA), Indole-3-butyric acid (IBA), Indole Acetic Acid (IAA), phloroglucinol and coumarin. The concentrations for each treatment were 10.0, 50.0 and 100.0 microM. After treatments the rhizome cuttings were planted in polybags containing forest soil and kept under different temperature regimes i.e., inside polyhose (at 20-25 degrees C), inside mist chamber (at 15-20 degrees C) and under nethouse (nursery condition, at 14-18 degrees C). The maximum rooting percentage (74.06%) was achieved at 20-25 degrees C (inside polyhouse) by applying 50.0 microM IBA. Inside poly house condition, the various developmental parameters showed better responses compare to other conditions. On the basis of present study emphasizes that the temperature play a crucial role in rooting and further growth of the plants in this species. By using this simple and significant conventional method of propagation we could be propagate this vulnerable medicinal and aromatic species at large scale for commercial purpose.
  • Effect of plant growth regulators (PGRs) on micropropagation of a vulnerable and high value medicinal plant Hedychium spicatum
    African Journal of Biotechnology, 2011
  • A review account on medicinal value of Hedychium spicatum Buch-Ham ex Sm: Vulnerable medicinal plant
    Journal of Medicinal Plants Research, 2010
  • Combined effect of PGRs and soil facilitate early flowering of an endangered alpine orchid Dactylorhiza hatagirea at lower elevation
    Current Science, 2010