Diogo Francisco Mauricio Coelho

@cbma.uminho.pt

Centre of Molecular and Environmental Biology, University of Minho
Centre of Molecular and Environmental Biology, University of Minho

Diogo Francisco Mauricio Coelho


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https://researchid.co/diogocoelho

RESEARCH, TEACHING, or OTHER INTERESTS

Biochemistry, Genetics and Molecular Biology, Biotechnology, Genetics, Biochemistry
33

Scopus Publications

1657

Scholar Citations

19

Scholar h-index

26

Scholar i10-index

Scopus Publications

  • Simplified, High Yielding Extraction of Xylan/Xylo-Oligosaccharides from Palmaria palmata: The Importance of the Algae Preservation Treatment
    Diogo Coelho, Diogo Félix Costa, Mário Barroca, Sara Alexandra Cunha, Maria Manuela Pintado, et al.
    Marine Drugs, 2025
    The complex plant cell wall heteropolysaccharide xylan, and its breakdown products xylo-oligosaccharides and xylose, are value-added compounds with a plethora of potential applications in diverse areas. They are nonetheless currently poorly exploited, with a major bottleneck being the unavailability of efficient, low-cost, high-yield production processes. The major objective of the present study is to identify and characterise a high-yield process for the preparation of highly pure xylan/XOS products from the macroalga Palmaria palmata. Currently, most xylan is extracted from land-sourced lignocellulosic feedstocks, but we take advantage of the high xylan content, xylan aqueous solubility, lignin-free nature, weakly linked cell wall matrix, and sustainability of the macroalga to identify a simple, sustainable, high-yield, novel-xylan-structure extraction process. This is composed of five steps: alga oven drying, milling, aqueous extraction, centrifugation, and dialysis, and we show that the alga preservation step plays a critical role in component extractability, with oven drying at high temperatures, ~100 °C, enhancing the subsequent aqueous extraction process, and providing for xylan yields as high as 80% of a highly pure (~90%) xylan product. The process developed herein and the insights gained will promote a greater availability of these bioactive compounds and open up their application potential.
  • Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets
    David Miguel Ribeiro, Diogo Coelho, Mónica Costa, Daniela Filipa Pires Carvalho, Céline C. Leclercq, et al.
    Scientific Reports, 2024
    Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics (RNA-seq) and proteomics (LC–MS) approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation may have contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintaining tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects of cell wall degradation during digestion.
  • Tissue-Specific Variation in Aquaporins and Cytokines Transcriptional Profiles in Piglets being LPS Challenged
    Inês V. da Silva, Diogo Coelho, José António Mestre Prates, Graça Soveral, Paula A. Lopes
    Frontiers in Bioscience Landmark, 2023
    BACKGROUND Lipopolysaccharide (LPS), an effective stimulator of the immune system, has been widely applied in an experimental pig model for human sepsis. Aquaporins (AQPs), a family of small integral membrane proteins responsible for facilitating water fluxes through the cell membrane, offer potential promising drug targets for sepsis treatment due to their role in water balance and inflammation. METHODS In order to investigate the potential effect of a dietary amino acid mixture supplementation on LPS-challenged weaned piglets, a total of 30, 28-day-old, males were randomly allocated to 1 of 3 dietary treatments for a 5-week period, with 10 animals in each: diet 1 was a control (CTL) treatment; diet 2 was LPS treatment, where the piglets were intraperitoneally administered LPS (at 25 µg/kg body weight); diet 3 was LPS + cocktail treatment, where the piglets were intraperitoneally administered LPS and fed a diet supplemented with a mixture of arginine, branched-chain amino acids (BCAA, leucine, valine, and isoleucine), and cystine. Key organs that control sepsis were collected and processed by real time quantitative PCR (RT-qPCR) for the AQPs and cytokines transcriptional profiles. RESULTS Minor variations were detected for AQPs and inflammatory markers mRNA levels, upon the dependence of LPS or the amino acid cocktail suggesting the piglets' immune recovery. Using a discriminant analysis tool, we report for the first time, a tissue-specific variation in AQPs and cytokines transcriptional profiles that clearly distinguish the small intestine and the kidney from the liver and the spleen. CONCLUSIONS This study provides a novel insight into the gene expression signature of AQPs and cytokines in the functional physiology of each organ in piglets.
  • Microalgae application in feeds for monogastrics
    Mónica Costa, Diogo Coelho, Cristina Alfaia, José Pestana, Paula A. Lopes, et al.
    Handbook of Food and Feed from Microalgae Production Application Regulation and Sustainability, 2023
  • Integrated Omics analysis of pig muscle metabolism under the effects of dietary Chlorella vulgaris and exogenous enzymes
    Diogo Coelho, David Ribeiro, Hugo Osório, André Martinho de Almeida, José António Mestre Prates
    Scientific Reports, 2022
    Monogastric feeding is dependent on costly conventional feedstuffs. Microalgae such as Chlorella vulgaris are a sustainable alternative; however, its recalcitrant cell wall hinders monogastric digestion. Carbohydrate Active Enzyme (CAZyme) supplementation is a possible solution. The objective of this work was to evaluate the effect of 5% dietary C. vulgaris (CV) and enzymatic supplementation (CV + R—Rovabio® Excel AP; CV + M—four CAZyme mix) on muscle transcriptome and proteome of finishing pigs, in an integrated approach. Control pigs increased the abundance of contractile apparatus (MYH1, MYH2, MYH4) and energy metabolism (CKMT1, NDUFS3) proteins, demonstrating increased nutrient availability. They had increased expression of SCD, characteristic of increased glucose availability, via the activation of SREBP-1c and ChREBP. CV and CV + R pigs upregulated proteolytic and apoptotic genes (BAX, DDA1), whilst increasing the abundance of glucose (UQCRFS1) and fatty acid catabolism (ACADS) proteins. CV + R pigs upregulated ACOT8 and SIRT3 genes as a response to reduced nutrient availability, maintaining energy homeostasis. The cell wall specific CAZyme mix, CV + M, was able to comparatively reduce Omics alterations in the muscle, thereby reducing endogenous nutrient catabolism compared to the CV + R and CV.
  • Combined effects of dietary Laminaria digitata with alginate lyase on plasma metabolites and hepatic lipid, pigment and mineral composition of broilers
    Mónica Mendes Costa, Paula Alexandra Lopes, José Miguel Pestana Assunção, Cristina Maria Riscado Pereira Mate Alfaia, Diogo Francisco Maurício Coelho, et al.
    BMC Veterinary Research, 2022
    Background The Laminaria digitata is an abundant macroalga and a sustainable feedstock for poultry nutrition. L. digitata is a good source of essential amino acids, carbohydrates and vitamins, including A, D, E, and K, as well as triacylglycerols and minerals, in particular iron and calcium. However, the few studies available in the literature with broilers document the application of this macroalga as a dietary supplement rather than a feed ingredient. No study has addressed up until now the effects of a high-level incorporation (> 2% in the diet) of L. digitata on plasma biochemical markers and hepatic lipid composition, as well as minerals and pigments profile in the liver of broilers. Our experimental design included one hundred and twenty Ross 308 male birds contained in 40 wired-floor cages and distributed to the following diets at 22 days of age (n = 10) for 15 days: 1) a corn-soybean basal diet (Control); 2) the basal diet plus 15% of L. digitata (LA); 3) the basal diet plus 15% of L. digitata with 0.005% of Rovabio® Excel AP (LAR); and 4) the basal diet plus 15% of L. digitata with 0.01% of the recombinant CAZyme, alginate lyase (LAE). Results L. digitata compromised birds’ growth performance by causing a reduction in final body weight. It was found an increase in hepatic n-3 and n-6 fatty acids, in particular C18:2n-6, C18:3n-6, C20:4n-6, C20:5n-3, C22:5n-3 and C22:6n-3 with the addition of the macroalga, with or without feed enzymes, to the broiler diets. Also, the beneficial C18:3n-3 fatty acid was increased by combining L. digitata and commercial Rovabio® Excel AP compared to the control diet. The sum of SFA, MUFA and the n-6/n-3 PUFA ratio were decreased by L. digitata, regardless the addition of exogenous enzymes. β-carotene was enhanced by L. digitata, individually or combined with CAZymes, being also responsible for a positive increase in total pigments. Macrominerals, in particular phosphorous and sulphur, were increased in the liver of broilers fed L. digitata individually relative to the control. For microminerals, copper, iron and the correspondent sum were consistently elevated in the liver of broilers fed L. digitata, individually or combined with exogenous CAZymes. The powerful discriminant analysis tool based on the hepatic characterization revealed a good separation between the control group and L. digitata diets but failed to discriminate the addition of feed enzymes. Conclusions Overall, this study highlights the value of L. digitata as a feed ingredient for the poultry industry. Moreover, we can conclude that the effect of L. digitata overpowers the effect of feed enzymes, both the Rovabio® Excel AP and the alginate lyase. Having in mind the negative effects observed on birds’ performance, our main recommendation at this stage is to restraint L. digitata incorporation level in forthcoming nutritional studies.
  • Influence of Chlorella vulgaris on growth, digestibility and gut morphology and microbiota of weaned piglet
    Cátia F. Martins, Paolo Trevisi, Diogo F. Coelho, Federico Correa, David M. Ribeiro, et al.
    Scientific Reports, 2022
    The purpose of this study was to evaluate the impact of Chlorella vulgaris (5% in the diet), supplemented or not with two exogenous carbohydrase mixtures on piglets’ performance, nutrient digestibility and gut morphology, fermentation products and microbiota. Forty-four male piglets weaned at 28 days of age, with 11.2 ± 0.46 kg of live weight, were used and assigned to 1 of 4 dietary treatments: cereal and soybean meal based-diet (control, n = 11), control diet with 5% of C. vulgaris (CH, n = 10), CH diet supplemented with 0.005% of Rovabio® Excel AP (CH + R, n = 10) and CH diet supplemented with 0.01% of a recombinant 4-carbohydrase mixture (CH + M, n = 11). Growth performance was not changed by the of C. vulgaris inclusion during 21 days of trial. However, total tract apparent digestibility of nutritional fractions was negatively impacted by the inclusion. In addition, the viscosity of duodenum plus jejunum contents slightly increased in all groups fed with the microalga. In contrast, dietary microalga increased duodenum villus height and promoted a healthier gut microbiota, with higher abundance of some specific bacterial taxa (Colidextribacter, Oscillospira and Lactobacillus). This study indicates that the dietary inclusion of 5% C. vulgaris improves piglets’ gut health without impairing performance. Data also indicate that C. vulgaris reduces nutrient digestibility but promotes compensatory developments of gut mucosa and prebiotic effects. Dietary supplementation with exogenous carbohydrases does not seem to be necessary for this inclusion level. Therefore, the incorporation of CH as a sustainable feed ingredient in piglets’ nutrition is a viable alternative approach.
  • Impact of dietary Chlorella vulgaris and feed enzymes on health status, immune response and liver metabolites in weaned piglets
    Cátia F. Martins, Paula A. Lopes, Mariana Palma, Rui M. A. Pinto, Mónica Costa, et al.
    Scientific Reports, 2022
    In this study, we analysed the impact of dietary inclusion of Chlorella vulgaris and carbohydrases on general health, redox status, immune response, liver lipids and metabolites in weaned piglets. Forty-four male piglets were allocated into four diets: control (n = 11), CH (control diet with 5% CH, n = 10), CH+R (control diet with 5% CH plus 0.005% Rovabio Excel AP, n = 10), and CH+M (control diet with 5% CH plus 0.01% of a pre-selected four-CAZyme mixture, n = 11). After 15 days of trial, animals were slaughtered and samples of blood and liver collected. Spectrophotometry methods and commercial kits were used to determine blood parameters and gas and liquid chromatography for hepatic fatty acid and chlorophylls profiles, respectively. While total, LDL- and VLDL-cholesterol were increased by CH, the opposite was recorded for HDL-cholesterol (p < 0.001). Piglets fed CH-based diets presented an increase of IgG and a decrease of IgM (p < 0.001) which along with lymphocytes exacerbation contributed for piglets’ survival after weaning. n−6 PUFA were reduced in piglets fed CH and the opposite occurred for n−3 PUFA (p < 0.001), thus benefiting n−6/n−3 ratio in the liver. Chlorophylls amount was not changed by the use of Rovabio or enzymatic mixture. The discriminant analysis applied to hepatic parameters revealed a clear separation between control and CH-based diets but failed to discriminate feed enzymes. Our findings indicate health promoting effects of CH as feed ingredient in piglets’ nutrition at weaning, without negatively impacting on animals’ performance.
  • Dietary Chlorella vulgaris with a specific enzyme mixture enriches pork in potassium and improves its sodium to potassium ratio
    Mónica Costa, Marta Madeira, Diogo Coelho, Cátia Falcão, Miguel Mourato, et al.
    British Food Journal, 2022
    PurposeThe purpose of this study was to assess the effect of dietary inclusion of 5% Chlorella vulgaris, individually or supplemented with two carbohydrase mixtures, on pork's mineral profile (calcium, copper, iron, potassium, magnesium, manganese, sodium, phosphorous, sulfur and zinc).Design/methodology/approachForty male finishing pigs were assigned to four dietary treatments: cereal and soybean meal-based diet (control), control diet with 5% microalga (CCV), CCV diet supplemented with 0.005% of a commercial xylanase and β-glucanase-based complex and CCV diet supplemented with 0.01% of a mixture of four carbohydrate-active enzymes. Pigs were slaughtered after 41 ± 7.8 days of trial and the mineral composition of longissimus lumborum muscle was analyzed using inductively coupled plasma–optical emission spectrometry.FindingsWhen C. vulgaris was supplemented with a four-CAZyme mixture, an increase of total minerals in meat was found due to a higher content of potassium, which led to a decrease of sodium to potassium ratio in pork. However, CCV treatment decreased the amount of calcium and manganese in meat, which was likely due to a lower bioavailability of these minerals in the diet.Originality/valueConsidering the imbalance of sodium and potassium in most Western diets and the increasing occurrence of cardiovascular diseases in the population, the improvement of meat quality caused by a reduction of sodium to potassium ratio might help to reduce the prevalence of high blood pressure and cardiovascular diseases, having thus benefits for consumers' health.
  • Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
    Mónica M. Costa, Luís B. Pio, Pedro Bule, Vânia A. Cardoso, Marlene Duarte, et al.
    Animal Nutrition, 2022
  • Testimony on a successful lab protocol to disrupt Chlorella vulgaris microalga cell wall
    Paula A. Lopes, Diogo Coelho, José A. M. Prates
    Plos One, 2022
  • Impact of Chlorella vulgaris as feed ingredient and carbohydrases on the health status and hepatic lipid metabolism of finishing pigs
    Diogo Coelho, Cristina M. Alfaia, Paula A. Lopes, José M. Pestana, Mónica M. Costa, et al.
    Research in Veterinary Science, 2022
  • Effect of Dietary Laminaria digitata with Carbohydrases on Broiler Production Performance and Meat Quality, Lipid Profile, and Mineral Composition
    Mónica M. Costa, José M. Pestana, Diogo Osório, Cristina M. Alfaia, Cátia F. Martins, et al.
    Animals, 2022
  • Influence of Feeding Weaned Piglets with Laminaria digitata on the Quality and Nutritional Value of Meat
    David Ribeiro, Cristina Alfaia, José Pestana, Daniela Carvalho, Mónica Costa, et al.
    Foods, 2022
  • Effect of dietary incorporation of Chlorella vulgaris and CAZyme supplementation on the hepatic proteome of finishing pigs
    David M. Ribeiro, Diogo Coelho, Hugo Osório, Cátia Martins, João P. Bengala Freire, et al.
    Journal of Proteomics, 2022
  • Impact of dietary Chlorella vulgaris and carbohydrate-active enzymes incorporation on plasma metabolites and liver lipid composition of broilers
    Diogo Francisco Maurício Coelho, Cristina Maria Riscado Pereira Mate Alfaia, José Miguel Pestana Assunção, Mónica Costa, Rui Manuel Amaro Pinto, et al.
    BMC Veterinary Research, 2021
  • Using microalgae as a sustainable feed resource to enhance quality and nutritional value of pork and poultry meat
    Cátia F. Martins, David M. Ribeiro, Mónica Costa, Diogo Coelho, Cristina M. Alfaia, et al.
    Foods, 2021
  • An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall
    Mónica Costa, Luís Pio, Pedro Bule, Vânia Cardoso, Cristina M. Alfaia, et al.
    Scientific Reports, 2021
  • Quality traits and nutritional value of pork and poultry meat from animals fed with seaweeds
    David Miguel Ribeiro, Cátia Falcão Martins, Mónica Costa, Diogo Coelho, José Pestana, et al.
    Foods, 2021
  • Effect of dietary inclusion of Spirulina on production performance, nutrient digestibility and meat quality traits in post-weaning piglets
    Cátia Falcão Martins, José Pestana Assunção, David M. Ribeiro Santos, Marta Sofia Morgado dos S. Madeira, Cristina Maria Riscado Pereira Mateus Alfaia, et al.
    Journal of Animal Physiology and Animal Nutrition, 2021
  • Influence of dietary Chlorella vulgaris and carbohydrate-active enzymes on growth performance, meat quality and lipid composition of broiler chickens
    C.M. Alfaia, J.M. Pestana, M. Rodrigues, D. Coelho, M.J. Aires, et al.
    Poultry Science, 2021
  • Effects of chlorella vulgaris as a feed ingredient on the quality and nutritional value of weaned piglets’ meat
    Cátia F. Martins, José M. Pestana, Cristina M. Alfaia, Mónica Costa, David M. Ribeiro, et al.
    Foods, 2021
  • A high dietary incorporation level of Chlorella vulgaris improves the nutritional value of pork fat without impairing the performance of finishing pigs
    Diogo Coelho, José Pestana, João M. Almeida, Cristina M. Alfaia, Carlos M. G. A. Fontes, et al.
    Animals, 2020
  • Ameliorating pork marbling and quality with novel feeding approaches
    M. S. Madeira, C. M. Alfaia, P. A. Lopes, J. Pestana, D. Coelho, et al.
    Advances in Animal Health Medicine and Production A Research Portrait of the Centre for Interdisciplinary Research in Animal Health Ciisa University of Lisbon Portugal, 2020
  • A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets
    Diogo Coelho, Paula A. Lopes, Vânia Cardoso, Patrícia Ponte, Joana Brás, et al.
    Journal of Animal Physiology and Animal Nutrition, 2020
  • Novel combination of feed enzymes to improve the degradation of Chlorella vulgaris recalcitrant cell wall
    Diogo Coelho, Paula A. Lopes, Vânia Cardoso, Patrícia Ponte, Joana Brás, et al.
    Scientific Reports, 2019
  • Pork byproducts
    Cristina Mateus Alfaia, Marta S. Madeira, José Pestana, Diogo Coelho, Paula A. Lopes, et al.
    Byproducts from Agriculture and Fisheries Adding Value for Food Feed Pharma and Fuels, 2019
  • Current feeding strategies to improve pork intramuscular fat content and its nutritional quality
    C.M. Alfaia, P.A. Lopes, M.S. Madeira, J.M. Pestana, D. Coelho, et al.
    Advances in Food and Nutrition Research, 2019
  • Effect of glutamine and/or cystine on gut health, immunity and redox status in post-weaning piglets
    J. Freire, J. Prates, M. Pinho, PA. Lopes, M. Madeira, et al.
    Eaap Scientific Series, 2019
  • From natural triacylglycerols to novel structured lipids containing n-3 long-chain polyunsaturated fatty acids
    Paula A. Lopes, José M. Pestana, Diogo Coelho, Marta S. Madeira, Cristina M. Alfaia, et al.
    Molecular Nutrition of Fats, 2018
  • Microalgae as feed ingredients for livestock production and meat quality: A review
    Marta S. Madeira, Carlos Cardoso, Paula A. Lopes, Diogo Coelho, Cláudia Afonso, et al.
    Livestock Science, 2017
  • Arginine supplementation modulates pig plasma lipids, but not hepatic fatty acids, depending on dietary protein level with or without leucine
    Marta Sofia Morgado dos Santos Madeira, Eva Sofia Alves Rolo, Virgínia Maria Rico Pires, Cristina Maria Riscado Pereira Mate Alfaia, Diogo Francisco Maurício Coelho, et al.
    BMC Veterinary Research, 2017
  • Reduced protein diets increase intramuscular fat of psoas major, a red muscle, in lean and fatty pig genotypes
    M.S. Madeira, P.A. Lopes, P. Costa, D. Coelho, C.M. Alfaia, et al.
    Animal, 2017

RECENT SCHOLAR PUBLICATIONS

  • Simplified, High Yielding Extraction of Xylan/Xylo-Oligosaccharides from Palmaria palmata : The Importance of the Algae Preservation Treatment
    D Coelho, DF Costa, M Barroca, SA Cunha, MM Pintado, H Abreu, ...
    Marine Drugs 23 (8), 302 , 2025
    2025
    Citations: 3
  • Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets
    DM Ribeiro, D Coelho, M Costa, DFP Carvalho, CC Leclercq, J Renaut, ...
    Scientific Reports 14 (1), 4589 , 2024
    2024
    Citations: 13
  • Tissue-Specific Variation in Aquaporins and Cytokines Transcriptional Profiles in Piglets being LPS Challenged
    IV da Silva, D Coelho, JAM Prates, G Soveral, PA Lopes
    Frontiers in Bioscience-Landmark 28 (6), 126 , 2023
    2023
    Citations: 3
  • Effetti di Chlorella vulgaris su crescita, attività digestiva, morfologia intestinale e microbiota dei suinetti svezzati.
    CF Martins, P Trevisi, DF Coelho, F Correa, DM Ribeiro, CM Alfaia, ...
    Summa, Animali da Reddito 18 (5) , 2023
    2023
  • Handbook of food and feed from microalgae
    M Costa, D Coelho, C Alfaia, J Pestana, PA Lopes, JAM Prates
    Academic Press , 2023
    2023
  • Microalgae application in feeds for monogastrics
    M Costa, D Coelho, C Alfaia, J Pestana, PA Lopes, JAM Prates
    Handbook of Food and Feed from Microalgae, 411-420 , 2023
    2023
    Citations: 10
  • Dietary Chlorella vulgaris with a specific enzyme mixture enriches pork in potassium and improves its sodium to potassium ratio
    M Costa, M Madeira, D Coelho, C Falcão, M Mourato, JA Mestre Prates
    British Food Journal 124 (12), 4644-4652 , 2022
    2022
    Citations: 3
  • Integrated Omics analysis of pig muscle metabolism under the effects of dietary Chlorella vulgaris and exogenous enzymes
    D Coelho, D Ribeiro, H Osório, AM de Almeida, JAM Prates
    Scientific Reports 12 (1), 16992 , 2022
    2022
    Citations: 19
  • Impact of dietary Chlorella vulgaris and feed enzymes on health status, immune response and liver metabolites in weaned piglets
    CF Martins, PA Lopes, M Palma, RMA Pinto, M Costa, CM Alfaia, ...
    Scientific Reports 12 (1), 16816 , 2022
    2022
    Citations: 20
  • Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
    MM Costa, LB Pio, P Bule, VA Cardoso, M Duarte, CM Alfaia, DF Coelho, ...
    Animal Nutrition 9, 184-192 , 2022
    2022
    Citations: 43
  • Testimony on a successful lab protocol to disrupt Chlorella vulgaris microalga cell wall
    PA Lopes, D Coelho, JAM Prates
    Plos one 17 (5), e0268565 , 2022
    2022
    Citations: 15
  • Impact of Chlorella vulgaris as feed ingredient and carbohydrases on the health status and hepatic lipid metabolism of finishing pigs
    D Coelho, CM Alfaia, PA Lopes, JM Pestana, MM Costa, RMA Pinto, ...
    Research in Veterinary Science 144, 44-53 , 2022
    2022
    Citations: 19
  • Combined effects of dietary Laminaria digitata with alginate lyase on plasma metabolites and hepatic lipid, pigment and mineral composition of broilers
    MM Costa, PA Lopes, JMP Assunção, CMRPM Alfaia, DFM Coelho, ...
    BMC Veterinary Research 18 (1), 153 , 2022
    2022
    Citations: 5
  • Effect of Dietary Laminaria digitata with Carbohydrases on Broiler Production Performance and Meat Quality, Lipid Profile, and Mineral Composition
    MM Costa, JM Pestana, D Osório, CM Alfaia, CF Martins, M Mourato, ...
    Animals 12 (8), 1007 , 2022
    2022
    Citations: 23
  • Influence of Chlorella vulgaris on growth, digestibility and gut morphology and microbiota of weaned piglet
    CF Martins, P Trevisi, DF Coelho, F Correa, DM Ribeiro, CM Alfaia, ...
    Scientific Reports 12 (1), 6012 , 2022
    2022
    Citations: 45
  • Influence of Chlorella vulgaris on growth, digestibility and gut morphology and microbiota of weaned piglet
    CF Martins, P Trevisi, DF Coelho, F Correa, DM Ribeiro, CM Alfaia, ...
    Scientific Reports 12 (1), 6012 , 2022
    2022
    Citations: 45
  • Influence of Feeding Weaned Piglets with Laminaria digitata on the Quality and Nutritional Value of Meat
    DM Ribeiro, CM Alfaia, JM Pestana, DFP Carvalho, M Costa, CF Martins, ...
    Foods 11 (7), 1024 , 2022
    2022
    Citations: 20
  • Effect of dietary incorporation of Chlorella vulgaris and CAZyme supplementation on the hepatic proteome of finishing pigs
    DM Ribeiro, D Coelho, H Osório, C Martins, JPB Freire, J Almeida, ...
    Journal of Proteomics 256, 104504 , 2022
    2022
    Citations: 16
  • Improving the Nutritional Value of Microalgae for Feeding Pigs Through the Use of Novel Enzymes
    DFM Coelho
    PQDT-Global , 2022
    2022
    Citations: 1
  • Quality traits and nutritional value of pork and poultry meat from animals fed with seaweeds
    DM Ribeiro, CF Martins, M Costa, D Coelho, J Pestana, C Alfaia, ...
    Foods 10 (12), 2961 , 2021
    2021
    Citations: 57

MOST CITED SCHOLAR PUBLICATIONS

  • Microalgae as feed ingredients for livestock production and meat quality: A review
    MS Madeira, C Cardoso, PA Lopes, D Coelho, C Afonso, NM Bandarra, ...
    Livestock science 205, 111-121 , 2017
    2017
    Citations: 608
  • Influence of dietary Chlorella vulgaris and carbohydrate-active enzymes on growth performance, meat quality and lipid composition of broiler chickens
    CM Alfaia, JM Pestana, M Rodrigues, D Coelho, MJ Aires, DM Ribeiro, ...
    Poultry science 100 (2), 926-937 , 2021
    2021
    Citations: 115
  • Using microalgae as a sustainable feed resource to enhance quality and nutritional value of pork and poultry meat
    CF Martins, DM Ribeiro, M Costa, D Coelho, CM Alfaia, M Lordelo, ...
    Foods 10 (12), 2933 , 2021
    2021
    Citations: 99
  • Novel combination of feed enzymes to improve the degradation of Chlorella vulgaris recalcitrant cell wall
    D Coelho, PA Lopes, V Cardoso, P Ponte, J Brás, MS Madeira, CM Alfaia, ...
    Scientific Reports 9 (1), 5382 , 2019
    2019
    Citations: 91
  • Current feeding strategies to improve pork intramuscular fat content and its nutritional quality
    CM Alfaia, PA Lopes, MS Madeira, JM Pestana, D Coelho, F Toldrá, ...
    Advances in food and nutrition research 89, 53-94 , 2019
    2019
    Citations: 66
  • A two‐enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets
    D Coelho, PA Lopes, V Cardoso, P Ponte, J Brás, MS Madeira, CM Alfaia, ...
    Journal of animal physiology and animal nutrition 104 (1), 310-321 , 2020
    2020
    Citations: 63
  • Quality traits and nutritional value of pork and poultry meat from animals fed with seaweeds
    DM Ribeiro, CF Martins, M Costa, D Coelho, J Pestana, C Alfaia, ...
    Foods 10 (12), 2961 , 2021
    2021
    Citations: 57
  • A High Dietary Incorporation Level of Chlorella vulgaris Improves the Nutritional Value of Pork Fat without Impairing the Performance of Finishing Pigs
    D Coelho, J Pestana, JM Almeida, CM Alfaia, CMGA Fontes, O Moreira, ...
    Animals 10 (12), 2384 , 2020
    2020
    Citations: 50
  • Effect of dietary inclusion of Spirulina on production performance, nutrient digestibility and meat quality traits in post‐weaning piglets
    CF Martins, J Pestana Assuncao, DM Ribeiro Santos, MSMS Madeira, ...
    Journal of Animal Physiology and Animal Nutrition 105 (2), 247-259 , 2021
    2021
    Citations: 49
  • Influence of Chlorella vulgaris on growth, digestibility and gut morphology and microbiota of weaned piglet
    CF Martins, P Trevisi, DF Coelho, F Correa, DM Ribeiro, CM Alfaia, ...
    Scientific Reports 12 (1), 6012 , 2022
    2022
    Citations: 45
  • Influence of Chlorella vulgaris on growth, digestibility and gut morphology and microbiota of weaned piglet
    CF Martins, P Trevisi, DF Coelho, F Correa, DM Ribeiro, CM Alfaia, ...
    Scientific Reports 12 (1), 6012 , 2022
    2022
    Citations: 45
  • Effects of Chlorella vulgaris as a Feed Ingredient on the Quality and Nutritional Value of Weaned Piglets’ Meat
    CF Martins, JM Pestana, CM Alfaia, M Costa, DM Ribeiro, D Coelho, ...
    Foods 10 (6), 1155 , 2021
    2021
    Citations: 45
  • Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
    MM Costa, LB Pio, P Bule, VA Cardoso, M Duarte, CM Alfaia, DF Coelho, ...
    Animal Nutrition 9, 184-192 , 2022
    2022
    Citations: 43
  • An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall
    M Costa, L Pio, P Bule, V Cardoso, CM Alfaia, D Coelho, J Brás, ...
    Scientific Reports 11 (1), 9706 , 2021
    2021
    Citations: 35
  • Impact of dietary Chlorella vulgaris and carbohydrate-active enzymes incorporation on plasma metabolites and liver lipid composition of broilers
    DFM Coelho, CMRPM Alfaia, JMP Assunção, M Costa, RMA Pinto, ...
    BMC Veterinary Research 17 (1), 229 , 2021
    2021
    Citations: 34
  • Reduced protein diets increase intramuscular fat of psoas major, a red muscle, in lean and fatty pig genotypes
    MS Madeira, PA Lopes, P Costa, D Coelho, CM Alfaia, JAM Prates
    animal 11 (11), 2094-2102 , 2017
    2017
    Citations: 26
  • Effect of Dietary Laminaria digitata with Carbohydrases on Broiler Production Performance and Meat Quality, Lipid Profile, and Mineral Composition
    MM Costa, JM Pestana, D Osório, CM Alfaia, CF Martins, M Mourato, ...
    Animals 12 (8), 1007 , 2022
    2022
    Citations: 23
  • Impact of dietary Chlorella vulgaris and feed enzymes on health status, immune response and liver metabolites in weaned piglets
    CF Martins, PA Lopes, M Palma, RMA Pinto, M Costa, CM Alfaia, ...
    Scientific Reports 12 (1), 16816 , 2022
    2022
    Citations: 20
  • Influence of Feeding Weaned Piglets with Laminaria digitata on the Quality and Nutritional Value of Meat
    DM Ribeiro, CM Alfaia, JM Pestana, DFP Carvalho, M Costa, CF Martins, ...
    Foods 11 (7), 1024 , 2022
    2022
    Citations: 20
  • Integrated Omics analysis of pig muscle metabolism under the effects of dietary Chlorella vulgaris and exogenous enzymes
    D Coelho, D Ribeiro, H Osório, AM de Almeida, JAM Prates
    Scientific Reports 12 (1), 16992 , 2022
    2022
    Citations: 19