Fungi associated with ornamental plants in some Nurseries in Al–Qurayyat, Jouf region, Saudi Arabia Mohamed Salah Massoud, Doaa Montaser Ahmed Khalil Iranian Journal of Microbiology, 2023 Background and Objectives: Fungi communities are important soil components as decomposers and plant symbionts, and they play an important part in natural ecological and biogeochemical processes. In this study, isolation and identification of terrestrial and zoosporic fungi were detected.Materials and Methods: Sixty–seven fungal species from thirty–four genera were isolated from 45 soil samples obtained randomly from nurseries in Al–Qurayyat, Jouf reagon, Saudi Arabia using the soil dilution technique on glucose–Czapek’s agar medium, cellulose–Czapek’s agar, and Potato dextrose agar medium.Authentic fungus manuals were then used to identify and characterise the mycoflora.Results: A total of 46 fungal species belonging to 22 terrestrial fungal genera were recovered on glucose–Czapek’s agar, 38 species belonging to 20 terrestrial fungal genera were recovered on cellulos–Czapek’s agar and 27 fungal species belonging to 15 terrestrial fungal genera were recovered on PDA medium while 12 species belonging to 7 genera zoosporic fungal genera were discovered.Conclusion: The most common terrestrial fungal genera were Aspergillus, Penicillium, Fusarium, Trichoderma, Acremonium, and Cladosopium while in zoosporic fungus. Allomyces was the most prevalent, followed by Achlya and Pythium.
Bioremoval capacity of phenol by some selected endophytic fungi isolated from hibiscus sabdariffa and batch biodegradation of phenol in paper and pulp effluents Iranian Journal of Microbiology, 2021
Phytochemical screening and antioxidant potential of endophytic fungi isolated from hibiscus sabdariffa Doaa M. A. Khalil, S. El-zayat, M. El-Sayed Journal of Applied Biotechnology Reports, 2020 Introduction: Fungal endophytes are friendly microbes colonizing in plants and play an effective role in plant-environment interactions. They produce valuable secondary metabolites that both plants and human beings can benefit from such products. Diverse compounds are biosynthesized by endophytes to promote plant growth and prevent pathogen attacks. Some of these compounds can be used for human drugs as antibiotics. Materials and Methods: Thirteen endophytic fungal species were isolated from root, stem and leaves of Hibiscus sabdariffa. The assessment of the antioxidants activity, total phenolic content (TPC), total flavonoids content and total antioxidant capacity (TAC), hydrogen peroxide scavenging and reducing power assays of 26 ethyl acetate and 13 methanol crude fungal extracts has been carried out. Qualitative assay of bioactive compounds for 39 endophytic fungal extracts have been checked by standard procedures. Quantitative estimation of TPC and flavonoids content was done. The DPPH free radical scavenging was estimated using DPPH (1,1-diphenyl-2-picrylhydrazyl), and hydrogen peroxide scavenging and reducing power assays were used to evaluate the antioxidants activity. Results: Qualitative detection of secondary metabolites revealed that endophytic fungal extracts from Aspergillus terreus isolated from the leaves of Hibiscus sabdariffa was found to be able to produce all the tested functional metabolites with the highest total phenolic contents (204.5±0.4 mg GAE/g) and antioxidant activity for DPPH radical scavenging assay (91.88 ± 0.17 μg/mL). Conclusions: The present study revealed that metabolites produced by some endophytic fungi isolated from the medicinal plant, Hibiscus sabdariffa could be a potential source of novel natural antioxidant compounds.
