Dr. Manoj Kumar

@online.gndu.ac.in

Scholar, Department of Microbiology
Guru Nanak Dev University

During my doctorate degree, my research is focused on bioremediation of organic pollutants and heavy metals using bacteria (aerobic and anaerobic) and metagenomics studies of polluted sites. This research experience has equipped me with a deep understanding of the microbiology field, as well as strong analytical and problem-solving skills. During my research I have worked on various instruments such as UV-Vis Spectroscopy, PCR, Ultra-sonicator, Gel-electrophoresis, SDS-PAGE, Lyophilizer, Fermentor, Rotary-evaporator, Gel-Doc, Anaerobic Chamber, Bioreactor development for degradation studies, HPLC,GC, LC-MS, XRD, SEM-EDS and AAS etc. Identification and characterization of microbes using NCBI, EzBioCloud and MG-RAST etc.

All the basic knowledge and techniques of microbiology laboratories.

EDUCATION

Graduation: B.Sc. (hons.) in Microbiology from Department of Microbiology, Shoolini Institute of Life Sciences and Business Management affiliated to Himachal Pradesh University (HPU), Shimla in 2010 with first grade (76 %).
Post-Graduation: M. Sc (hons.) Fermentation and Microbial Technology from Department of Microbiology, Guru Nanak Dev University, Amritsar (Punjab) in 2012 with first grade (75 %).
Qualified ASRB-ICAR NET in Agricultural Microbiology in 2016.
PhD. (Microbiology) degree has been officially conferred having specialization in Microbiology with thesis entitled "Evaluation of Bioremediation Potential of Culturable Bacterial Diversity of Polluted Sites and Assessment of their Unculturable Diversity" from Department of Microbiology, Guru Nanak Dev University (GNDU), Amritsar (Punjab) under the guidance of Prof (Dr.) Harvinder Singh Saini, Department of Microbiology, Dean-Faculty of Life Sciences, Guru Nanak Dev University, Amritsar (Punjab).

RESEARCH, TEACHING, or OTHER INTERESTS

Microbiology, Molecular Biology, Applied Microbiology and Biotechnology, Immunology and Microbiology

Scopus Publications

Scholar Citations

Scholar h-index

Scholar i10-index

Scopus Publications

Environmental impacts of air pollution and its abatement by plant species: A comprehensive review
Akshay Kumar Singh, Manoj Kumar, Kuldeep Bauddh, Ajai Singh, Pardeep Singh, Sughosh Madhav, and Sushil Kumar Shukla
Springer Science and Business Media LLC

Statistical and spectral analysis of wind over a strategic location
Nishi Srivastava, Jaya Divyam, Upamanyu Ghosh, Madhu Priya, Nisheeth Saxena, and Manoj Kumar
IWA Publishing
Abstract In the present work, we explored the inherent characteristics of the wind over a complex terrain site ‘Ranchi’ situated near a strategic location of the monsoon trough with various mathematical and statistical tools, i.e., time-series analysis, Fast Fourier transform (FFT), FFT coefficients, wavelet decomposition, and Weibull distribution. The time-series analysis showed a rapid day-to-day variability with a seasonal variation with a peak during summer. Fourier coefficients were concentrated for the winter/post-monsoon, indicating lower wind conditions, while wide spreads of the points indicate agility, i.e., high wind during the summer. The spectral features obtained using FFTs infer that wind has a prominent peak at a frequency f=0.00106724 (day−1) and f=0.00266809 (day−1). The power spectrum and wavelet decomposition show that the prominent frequencies correspond to yearly, eight, six, and four months. Weibull probability density function, cumulative probability distributions, and probability profiles are studied. Results show that the Weibull distribution function reasonably models the probability distribution of daily wind speed. Weibull scale parameter varied between 0.26 and 1.33 m/s, and the shape parameter ranged between 1.09 and 2.88. Results from various analyses indicate that the seasonal variation of wind speed over Ranchi is mainly associated with the development of monsoon trough over the site.

Harnessing phytomicrobiome signals for phytopathogenic stress management
Akanksha Sharma, Meenakshi Raina, Deepak Kumar, Archana Singh, Samira Chugh, Shalu Jain, Manoj Kumar, and Anjana Rustagi
Springer Science and Business Media LLC

Soil Enzymes and Their Role in Soil Health Improvement
Rini Rahul, Pallavi Sharma, Ashutosh Singh, Joginder Singh, and Manoj Kumar
Springer Nature Singapore

Role of microorganism in phytoremediation of mine spoiled soils
Ankit Abhilash Swain, Neha Dwivedi, Kuldeep Bauddh, and Manoj Kumar
Elsevier

Phytoremediation of heavy metal contaminated soil in association with arbuscular mycorrhizal fungi
Sonal Dixit, Preetanshika Tracy, Neha Vishnoi, Ankit Abhilash Swain, Kuldeep Bauddh, and Manoj Kumar
Elsevier

Screening for probiotic attributes of lactic acid bacteria isolated from human milk and evaluation of their anti-diabetic potentials
Manoj Kumar, Sukumaran Karthika, Nair Anjitha, Perumal Varalakshmi, and Balasubramaniem Ashokkumar
Informa UK Limited
ABSTRACT Lactic acid bacteria (LAB) have gained much attention recently due to their probiotic attributes with anti-ACE and anti-glycation potentials targeting management of diabetes. This study was designed to isolate LAB from human milk and to evaluate their potential for inhibitory activities on ACE and AGEs formation using in vitro assay models. Among 12 LAB isolates, many strains exhibited strong probiotic attributes including tolerance to low pH, high salt, gastric, and intestinal juices with higher cell adhesion. Further, cell-free extracts of LAB tested for in vitro anti-ACE and anti-AGEs activities evidenced Lacticaseibacillus casei MKU1, Lacticaseibacillus paracasei MKU2, Lactiplantibacillus pentosus MKU3, L. paracasei MKU7, L. delbrueckii GERU3 and Lactobacillus delbrueckii MKU10 as strong ACE and AGEs inhibitors. Subsequently, Principal Component Analysis showed a positive correlation with anti-ACE (p ≤ .01) and anti-glycation (p ≤ .05) activity of LAB. Overall, this study suggests that six Lactobacillus isolates of human milk with superior probiotic attributes looks promising as potential probiotic strains.

Microbial Cell Factories for the Biosynthesis of Vanillin and Its Applications
Sukumaran Karthika, Manoj Kumar, Santhalingam Gayathri, Perumal Varalakshmi, and Balasubramaniem Ashokkumar
Wiley

Ecological restoration of coal fly ash–dumped area through bamboo plantation
Raushan Kumar, Mohan Manu Thangaraju, Manoj Kumar, Sanjog Tarachand Thul, Vimal Chandra Pandey, Swati Yadav, Lal Singh, and Sunil Kumar
Springer Science and Business Media LLC

Exploring spatial and temporal trends of diurnal temperature range in the region of the Subarnarekha river basin India
Shashank Shree, Manoj Kumar, and Ajai Singh
Springer Science and Business Media LLC

Preface

Rhizobiont in Bioremediation of Hazardous Waste
Springer Singapore

An insight in bacteriophage based biosensors with focus on their detection methods and recent advancements
Simranjeet Singh, Daljeet Singh Dhanjal, Sonali, Siddharth Thotapalli, Vijay Kumar, Shivika Datta, Vineet Kumar, Manoj Kumar, and Joginder Singh
Elsevier BV

Herbicide glyphosate: Toxicity and microbial degradation
Simranjeet Singh, Vijay Kumar, Jatinder Pal Kaur Gill, Shivika Datta, Satyender Singh, Vaishali Dhaka, Dhriti Kapoor, Abdul Basit Wani, Daljeet Singh Dhanjal, Manoj Kumar,et al.
MDPI AG
Glyphosate is a non-specific organophosphate pesticide, which finds widespread application in shielding crops against the weeds. Its high solubility in hydrophilic solvents, especially water and high mobility allows the rapid leaching of the glyphosate into the soil leading to contamination of groundwater and accumulation into the plant tissues, therefore intricating the elimination of the herbicides. Despite the widespread application, only a few percentages of the total applied glyphosate serve the actual purpose, dispensing the rest in the environment, thus resulting in reduced crop yields, low quality agricultural products, deteriorating soil fertility, contributing to water pollution, and consequently threatening human and animal life. This review gives an insight into the toxicological effects of the herbicide glyphosate and current approaches to track and identify trace amounts of this agrochemical along with its biodegradability and possible remediating strategies. Efforts have also been made to summarize the biodegradation mechanisms and catabolic enzymes involved in glyphosate metabolism.

Evaluating metabolic potential of Thauera sp. M9 for the transformation of 4-chloroaniline (4-CA)
Manoj Kumar, Rohit Mahajan, and Harvinder Singh Saini
Elsevier BV

Detection of change over land use and land cover in Coimbatore urban area

Metagenomic analysis of the fecal microbiome of an adult elephant reveals the diversity of CAZymes related to lignocellulosic biomass degradation
Shaik Jakeer, Mahendra Varma, Juhi Sharma, Farnaz Mattoo, Dinesh Gupta, Joginder Singh, Manoj Kumar, and Naseem A. Gaur
Springer Science and Business Media LLC

Hippophae rhamnoides L. rhizobacteria exhibit diversified cellulase and pectinase activities
Pooja Bhadrecha, Madhu Bala, Yogender Pal Khasa, Anfal Arshi, Joginder Singh, and Manoj Kumar
Springer Science and Business Media LLC

In vitro plant breeding towards novel agronomic traits: Biotic and abiotic stress tolerance
Manoj Kumar, A. Muthusamy and Vivek Kumar
Springer Singapore

Reduction of hexavalent chromium (VI) by indigenous alkaliphilic and halotolerant Microbacterium sp. M5: comparative studies under growth and nongrowth conditions
M. Kumar and H.S. Saini
Oxford University Press (OUP)
To evaluate hexavalent chromium (Cr (VI)) reduction potential of indigenous isolate M5, under growing and nongrowing conditions.

Inheritance pattern of genes for morpho-physiological and yield traits in wheat (triticum aestivum L.)
J. Kumar, A. Kumar, M. Kumar, S.K. Singh, and L. Singh
Springer Science and Business Media LLC
In order to study the inheritance pattern of morpho-physiological traits in bread wheat, a 10×10 diallel cross, excluding reciprocals was made and grown in a randomized complete block design (RCBD) with three replications. Observations were recorded on Days to 75% flowering (DF), Days to maturity (DM), Duration of reproductive phase (DRP), Plant height (cm) (PH), Effective tiller/plant (TLS), No. of spikelets per spike (SLS), No. of grains per spike (GS), Grain weight per spike (g) (GW), Spike length (cm) (SL), Biological yield per plant (g) (BY), Harvest index (%) (HI), 1000-Grain weight (g) (TGW), Spike density (SD), Canopy temperature depression (°C) (CTD), Chlorophyll intensity (%) (CI), Chlorophyll fluorescence (Fv/Fm) (CF), Protein content (%) (PC), Grain yield per plant (g) (GY). Highly significant differences were observed among the genotypes for all traits. The resulted 45 F1s and their F2s used for study the nature of gene for grain yield and its contributing traits in bread wheat. The result indicated that considerable gene action and average degree of dominance respond to achieving significant result for grain yield and its component traits. In both the generations F1s and F2s, grain yield per plant (g) was governed by non-additive gene action based on combining ability analysis, (σ2 g/σ2 s)0.5 [GCA and SCA variance ratio] and (H1/D)0.5 [Degree of dominance] were exhibited over dominance type average degree of dominance for grain yield and its component traits in both generations. Genetic analyses of the traits confirm the involvement of both additive and non-additive gene effects in governing the inheritance.

Microbiome in Plant Health and Disease: Challenges and Opportunities
Vivek Kumar, R. Prasad, M. Kumar and D. Choudhary
Springer Singapore

Phytobiont and ecosystem restitution
Vivek Kumar, Manoj Kumar and R. Prasad
Springer Singapore

In silico approach for sustainable agriculture
D. Choudhary, Manoj Kumar, R. Prasad and Vivek Kumar
Springer Singapore

Solar radiation over four cities of India: Trend analysis using Mann-Kendall Test

RECENT SCHOLAR PUBLICATIONS

Novel Approaches to Alleviate Lignocellulosic Biomass Waste for Effective and Sustainable Utilization
D Agrawal, SK Saggu, S Devi, S Kumar, M Kumar
Value Addition and Utilization of Lignocellulosic Biomass: Through Novel 2025

Hydroxytyrosol in cancer research: recent and historical insights on discoveries and mechanisms of action
A Kumar, B Singh, K Paul, P Bakshi, P Bajaj, M Kumar, S Dhiman, ...
Future Journal of Pharmaceutical Sciences 10 (1), 129 2024

Deciphering Indigenous Bacterial Diversity of Co‐Polluted Sites to Unravel Its Bioremediation Potential: A Metagenomic Approach
M Kumar, HS Saini
Journal of Basic Microbiology 64 (9), e2400303 2024

Novel approaches for sustainable management of chromium contaminated wastewater
M Kumar, HS Saini
IntechOpen 2024

Origin and Management of Inorganic and Organic Contaminants
S Dhiman, S Kumar, M Kumar, G Kumar
Management and Mitigation of Emerging Pollutants 1, 21-67 2023

Insecticidal, genotoxic and biochemical effects of Shewanella sp. (SS4) and Thauera sp. (M9) on Spodoptera litura
S Sarkhandia, S Devi, G Sharma, M Kumar, S Koundal, P Chadha, ...
Journal of Applied Entomology 147 (9), 702-715 2023

Exploring the role of microbes for the management of persistent organic pollutants
M Kumar, SK Saggu, P Pratibha, SK Singh, S Kumar
Journal of Environmental Management 344, 118492 2023

Synergistic and additive interactions of Shewanella sp., Pseudomonas sp. and Thauera sp. with chlorantraniliprole and emamectin benzoate for controlling Spodoptera litura
HSSSK Sunaina Sarkhandia, Geetika Sharma, Rohit Mahajan, Satish Koundal ...
Scientific Reports 13, 14648 2023

Evaluating metabolic potential of Thauera sp. M9 for the transformation of 4-chloroaniline (4-CA)
M Kumar, R Mahajan, HS Saini
Biocatalysis and Agricultural Biotechnology 29, 101768 2020

Reduction of hexavalent chromium (VI) by indigenous alkaliphilic and halotolerant Microbacterium sp. M5: comparative studies under growth and nongrowth conditions
M Kumar, HS Saini
Journal of Applied Microbiology 127 (4), 1057-1068 2019

MOST CITED SCHOLAR PUBLICATIONS

Exploring the role of microbes for the management of persistent organic pollutants
M Kumar, SK Saggu, P Pratibha, SK Singh, S Kumar
Journal of Environmental Management 344, 118492 2023
Citations: 20

Evaluating metabolic potential of Thauera sp. M9 for the transformation of 4-chloroaniline (4-CA)
M Kumar, R Mahajan, HS Saini
Biocatalysis and Agricultural Biotechnology 29, 101768 2020
Citations: 19

Novel approaches for sustainable management of chromium contaminated wastewater
M Kumar, HS Saini
IntechOpen 2024
Citations: 5

Origin and Management of Inorganic and Organic Contaminants
S Dhiman, S Kumar, M Kumar, G Kumar
Management and Mitigation of Emerging Pollutants 1, 21-67 2023
Citations: 3

Deciphering Indigenous Bacterial Diversity of Co‐Polluted Sites to Unravel Its Bioremediation Potential: A Metagenomic Approach
M Kumar, HS Saini
Journal of Basic Microbiology 64 (9), e2400303 2024
Citations: 1