Biochemistry, Genetics and Molecular Biology, Pharmacology, Toxicology and Pharmaceutics, Agricultural and Biological Sciences, General Materials Science
Synthesis, Characterization, Acute Dermal Toxicity, Anti-inflammatory, and Wound Healing Potential of Biogenic Silver Nanoparticles in Balb C Mice Saiqa Andleeb, epub Iqbal, Nazia Gulzar, Abida Raza, Ashfaq Ahmad Current Pharmaceutical Biotechnology, 2024 Aim:The current study aimed to develop an economic plant-based therapeutic agent to improve the treatment strategies for diseases at the nano-scale.Method:In the current research, silver nanoparticles were synthesized using Trillium govanianum aqueous extract. Characterizations were done using UV–visible spectrophotometer, X-ray diffraction, scanning electron microscopy, and Fourier transform infrared spectroscopy. In vivo biological activities such as acute dermal toxicity, wound healing, and anti-inflammatory were done on Balb C mice. Absorbance at 295 nm corresponds to the out-of-plane quadrupole Plasmonresonance while at 350 nm corresponds to in-plane dipole resonance. SEM images showed the morphology of TGAgNPs is not exactly spherical while XRD analysis shows that highly crystalline TGAgNPs with an average size of 27.94 nm. The FTIR spectrum represents sharp peaks of aldehyde, amide I, aromatic rings, and polysaccharides. The microscopic assessment did not find any epidermal and dermal layer abnormalities in Blab C mice when exposed to TGAgNPs during acute dermal toxicity.Result & Discussion:Results revealed that 1000 mg/kg is not a lethal dose. In the wound healing activity, no mortality and no abnormal signs were observed when petroleum jelly, nitrofuranose, TGaqu, and TGAgNPs-based ointments were applied. Enhanced epithelization was recorded in TGaqu and TGAgNPs treated mice (p≤0.001). The wound contraction percentage was higher in nitrofuranose-treated mice (74%) followed by TGAgNPs (71%), and TGaqu (69%) compared to vehicle-treated and open-wounded mice. The paw edema model proved the potential use of TGAgNPs and TGaqu as anti-inflammatory agents.Conclusion:Hence, the results proved that both TGaqu and TGAgNPs are not toxic and possessed strong anti-inflammatory and wound-healing effects due to the presence of phytochemical constituents and could be used in various drug production as a therapeutic agent.
Fungus-mediated synthesis of Se-BiO-CuO multimetallic nanoparticles as a potential alternative antimicrobial against ESBL-producing Escherichia coli of veterinary origin Rida Rasheed, Bushra Uzair, Abida Raza, Reem Binsuwaidan, Nawaf Alshammari Frontiers in Cellular and Infection Microbiology, 2024 Bacterial infections emerge as a significant contributor to mortality and morbidity worldwide. Emerging extended-spectrum β-lactamase (ESBL) Escherichia coli strains provide a greater risk of bacteremia and mortality, are increasingly resistant to antibiotics, and are a major producer of ESBLs. E. coli bacteremia-linked mastitis is one of the most common bacterial diseases in animals, which can affect the quality of the milk and damage organ functions. There is an elevated menace of treatment failure and recurrence of E. coli bacteremia necessitating the adoption of rigorous alternative treatment approaches. In this study, Se-Boil-CuO multimetallic nanoparticles (MMNPs) were synthesized as an alternate treatment from Talaromyces haitouensis extract, and their efficiency in treating ESBL E. coli was confirmed using standard antimicrobial assays. Scanning electron microscopy, UV–visible spectroscopy, and dynamic light scattering were used to validate and characterize the mycosynthesized Se-BiO-CuO MMNPs. UV–visible spectra of Se-BiO-CuO MMNPs showed absorption peak bands at 570, 376, and 290 nm, respectively. The average diameters of the amorphous-shaped Se-BiO-CuO MMNPs synthesized by T. haitouensis extract were approximately 66–80 nm, respectively. Se-BiO-CuO MMNPs (100 μg/mL) showed a maximal inhibition zone of 18.33 ± 0.57 mm against E. coli. Se-BiO-CuO MMNPs also exhibited a deleterious impact on E. coli killing kinetics, biofilm formation, swimming motility, efflux of cellular components, and membrane integrity. The hemolysis assay also confirms the biocompatibility of Se-BiO-CuO MMNPs at the minimum inhibitory concentration (MIC) range. Our findings suggest that Se-BiO-CuO MMNPs may serve as a potential substitute for ESBL E. coli bacteremia.
Acute Toxicity, Anti-diabetic, and Anti-cancerous Potential of Trillium Govanianum-conjugated Silver Nanoparticles in Balb/c Mice Nazia Gulzar, Saiqa Andleeb, Abida Raza, Shaukat Ali, Iram Liaqat, et al. Current Pharmaceutical Biotechnology, 2024 Background: The current study aimed to develop an economic plant-based therapeutic agent to improve the treatment strategies for diseases at the nano-scale because Cancer and Diabetes mellitus are major concerns in developing countries. Therefore, in vitro and in vivo antidiabetic and anti-cancerous activities of Trillium govanianum conjugated silver nanoparticles were assessed. Methods: In the current study synthesis of silver nanoparticles using Trillium govanianum and characterization were done using a scanning electron microscope, UV-visible spectrophotometer, and FTIR analysis. The in vitro and in vivo anti-diabetic and anti-cancerous potential (200 mg/kg and 400 mg/kg) were carried out. Results: It was discovered that Balb/c mice did not show any major alterations during observation of acute oral toxicity when administered orally both TGaqu (1000 mg/kg) and TGAgNPs (1000 mg/kg), and results revealed that 1000 mg/kg is not lethal dose as did not find any abnormalities in epidermal and dermal layers when exposed to TGAgNPs. In vitro studies showed that TGAgNPs could not only inhibit alpha-glucosidase and protein kinases but were also potent against the brine shrimp. Though, a significant reduction in blood glucose levels and significant anti-cancerous effects was recorded when alloxan-treated and CCl4-induced mice were treated with TGAgNPs and TGaqu. Conclusion: Both in vivo and in vitro studies revealed that TGaqu and TGAgNPs are not toxic at 200 mg/kg, 400 mg/kg, and 1000 mg/kg doses and possess strong anti-diabetic and anti-cancerous effects due to the presence of phyto-constituents. Further, suggesting that green synthesized silver nanoparticles could be used in pharmaceutical industries to develop potent therapeutic agents.
Screening of Antibacterial, Anti-Biofilm, Cell Proliferation Inhibition, and Synergistic Effects of Biogenic Synthesized Silver Nanostructures Using Trillium govanianum with Antibiotics Journal of the Chemical Society of Pakistan, 2020
Analysis of complete and partial genome sequences of hepatitis b virus and determination of its genotypes and sub-genotypes from Pakistan Pakistan Journal of Zoology, 2016
Detection of HCV RNA and NS5A protein in peripheral blood mononuclear cells after sustained virological response may cause viral relapse Pakistan Journal of Zoology, 2015
Prevalent risk factors of HCV transmission in health care workers (HCWS) in Pakistan International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Genetic, biochemical, and immunological determinants of viral resistance to interferon alpha 2b combination therapy of HCV 3a infected Pakistani patients Life Science Journal, 2013
Modified glycol chitosan nanocarriers carry hydrophobic materials into tumours Nanotechnology 2010 Bio Sensors Instruments Medical Environment and Energy Technical Proceedings of the 2010 Nsti Nanotechnology Conference and Expo Nsti Nanotech 2010, 2010
Utility of PCR in diagnosis of problematic cases of typhoid Japanese Journal of Infectious Diseases, 2001
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MOST CITED SCHOLAR PUBLICATIONS
Green synthesis of silver nanoparticles via plant extracts: beginning a new era in cancer theranostics M Ovais, AT Khalil, A Raza, MA Khan, I Ahmad, NU Islam, M Saravanan, ... Nanomedicine 11 (23), 3157-3177 , 2016 2016 Citations: 429
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Selected hepatoprotective herbal medicines: Evidence from ethnomedicinal applications, animal models, and possible mechanism of actions M Ali, T Khan, K Fatima, QUA Ali, M Ovais, AT Khalil, I Ullah, A Raza, ... Phytotherapy research 32 (2), 199-215 , 2018 2018 Citations: 198
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Utility of PCR in diagnosis of problematic cases of typhoid A Haque, N Ahmed, A Peerzada, A Raza, S Bashir, G Abbas Japanese journal of infectious diseases 54 (6), 237-239 , 2002 2002 Citations: 60
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