Akhil Kumar Gupta
@luvas.edu.in
Scientist, Department of Veterinary Microbiology, LUVAS
Lala Lajpat Rai University of Veterinary and Animal Sciences
RESEARCH INTERESTS
Antibody production and Diagnostics
Scopus Publications
Scopus Publications
Savita Budania, Surinder Kumar Kadian, Karuppiah Kanagarajadurai, Vikas Yadav, Aman Kumar, and Akhil Kumar Gupta
Mary Ann Liebert Inc
Interleukin-17A is a pro-inflammatory cytokine that plays a key role in the immune response to many pathogens and implicated in autoimmune diseases. This molecule is also involved in providing protection to many bacterial and fungal infections of gastro-intestinal tract and respiratory mucosa. Although molecular aspect of IL-17A has been studied in few species, no data are available for buffalo, which is one of the major sources of milk production in India. Therefore, in the present study, IL-17A gene of Indian Murrah Buffalo origin was cloned, expressed, and analyzed using bioinformatic tools. The coding sequence of buffalo IL-17A gene was cloned in prokaryotic expression vector (pET-28a) followed by its expression, purification, and characterization. A computational analysis was performed to understand the sequence, structure, and evolutionary relationship of buIL-17A. It revealed that the length of buIL-17A sequence without signal peptide is 132 amino acids as in cattle. However, sequence identity is found to be 99% due to one amino substitution difference between buffalo and cattle. After analysis, it can be concluded that buIL-17A recombinant protein can be used as a potential immunobiological reagent for diagnostic and therapeutic purpose.
Vijay Kumar, Swati Dahiya, Savita Budania, Akhil Kumar Gupta, Punesh Sangwan, Anshul Lather, Pawan Kumar, Naresh Kumar Kakker, and Ajit Singh
Springer Science and Business Media LLC
RITU PANGHAL, SWATI DAHIYA, AKHIL KUMAR GUPTA, VISHAL SHARMA, YOGESH BANGAR, and NARESH KUMAR KAKKER
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Twelve apparently healthy and recently parturiated Murrah buffaloes, vaccinated with Foot-and-mouth disease (FMD) + Haemorrhagic septicaemia (HS) combined vaccine at an organized farm along with their newborn calves were inducted for detection of antibodies in serum and colostrum against FMD virus (FMDV) serotypes O, A and Asia-1, and Pasteurella multocida using enzyme linked immunosorbent assay. The calves born to vaccinated dams showed the presence of protective maternal antibody titre (≥1.8 log10) from birth till the period of study (16th week of age) against FMDV serotypes O, A and Asia-1. The maternal antibody titre against P. multocida were protective (≥1.8 log10) till fifth day of age which became partially protective thereafter till the period of study. It is recommended to avoid vaccination of the calves before 16th week of age with FMD+HS combined vaccine.
NEENA RUSTAGI, NARESH KUMAR KAKKER, SUMAN SHEORAN, PATIL CHANDRASHEKHAR SANTOSH, SWATI DAHIYA, and AKHIL KUMAR GUPTA
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
The present study reports kinetics of anti-P. multocida antibody (Ab) response at monthly intervals in Murrah buffaloes of different age groups vaccinated against combined Foot-and-mouth disease+haemorrhagic septicaemia (FMD+HS) vaccine. A total of 60 Murrah buffaloes of three age groups having 20 animals each: calves, heifers and adults were used to monitor anti-P. multocida Ab response at monthly intervals using single dilution indirect ELISA. The percentage of adult buffaloes protected were found to be the highest during all the six months post-vaccination followed by heifers and calves. The protective mean Ab titres were maintained up to six months post-vaccination for heifers and adults but not for calves. The F value (the ratio of two mean squares) for pre- and all the six month(s) post-vaccination and all the three age groups was significantly higher. Pearson Chi square value for pre-vaccination and all the six months except three months post-vaccination was significantly higher. Pearson correlation value was significantly higher with positive linear relationship. The data in the present study indicated that the combined FMD+HS vaccine was found to be effective in buffaloes of all age groups at government organised farm and could be an ideal approach in field conditions under Livestock Health Disease Control Program run by the Government of India.
Maneesh Sharma, C. S. Patil, Sandeep Saharan, Akhil Kumar Gupta, Snehil Gupta, and V. K. Jain
Springer Science and Business Media LLC
Rajpreet Kour, Parveen Kumar, Naresh Jindal, Sanjeevna Kumari Minhas, Ramesh Kumar, Akhil Kumar Gupta, and Anu Malik
Springer Science and Business Media LLC
Kanisht Batra, Trilok Nanda, Aman Kumar, Akhil Kumar Gupta, Rajni Kumari, Vinay Kumar, Nancy Sheoran, and Sushila Maan
Springer Science and Business Media LLC
Vinay Kumar, Sushila Maan, Aman Kumar, Kanisht Batra, Deepika Deepika, Anita Dalal, Akhil Gupta, Nitish Bansal, Nancy Sheoran, and N. Maan
Agricultural Research Communication Center
Brucellosis is one of the zoonotic diseases of major concern and can cause huge economic losses to livestock industry. Serological tests and bacterial isolation are considered as the gold standard assay for diagnosis of Brucella spp. but they are time-consuming, hazardous and lack specificity. To control and eradicate a disease, a confirmatory diagnostic method which is sensitive, quick and specific is the foremost requirement. Therefore in this study, we evaluated the performances of two newly designed TaqMan real-time PCR assays targeting the BruAB_0168 gene and BMEII0466 gene for Brucella abortus and Brucella melitensis (respectively). Both the assays were found to be highly specific in differentiation of respective species. Both the assays can detect as low as 0.02 fg of DNA and there was no detectable difference found in sensitivity of these two tests. R2 value and efficiency of these tests ranged from 0.992 - 0.998 and 100- 106%, respectively showing that these assays are highly efficient. Compared to conventional PCR assays these qPCR assays were 100 times higher sensitive. In conclusion, the present study showed that the developed real-time PCR assays are more sensitive, specific, have high reproducibility and repeatability and are faster than serological and conventional PCR methods for differentiation of Brucella abortus and Brucella melitensis.
Sushila Maan, Sangeeta Dalal, Aman Kumar, Anita Dalal, Nitish Bansal, Deepika Chaudhary, Akhil Gupta, and Narender Singh Maan
Springer Singapore
S. Maan, Aman Kumar, A. K. Gupta, A. Dalal, D. Chaudhary, T. K. Gupta, N. Bansal, V. Kumar, K. Batra, N. Sindhu,et al.
Wiley
Bluetongue (BT) and peste-des-petits-ruminants (PPR) are major transboundary diseases of small ruminant, which are endemic in India. Testing of bluetongue virus (BTV) and peste-des-petits-ruminants virus (PPRV) from recent outbreaks (2015-2016) in different regions of Haryana State of India revealed that 27.5% of the samples showed the presence of dual infection of BTV and PPRV. Analysis of Seg-2 of BTV (the serotype-determining protein) showed the presence of BTV-12w in several isolates. However, analysis of N gene fragment amplicons showed that viruses belong to lineage IV were most closely related to a pathogenic strain of PPRV from Delhi. This is the first report of co-circulation of PPRV lineage IV and bluetongue virus serotype 12 in the state.
S. Maan, N.S. Maan, K. Batra, A. Kumar, A. Gupta, Panduranga P. Rao, Divakar Hemadri, Yella Narasimha Reddy, M. Guimera, M.N. Belaganahalli,et al.
Elsevier BV
Lalit Kumar, Kanisht Batra, Deepika Chaudhary, Akhil Kumar Gupta, Anita Dalal, Brindha Kalyanaraman, Ganesan P. Irulappan, Vinay Kumar, and Sushila Maan
American Society for Microbiology
ABSTRACT The complete genome sequence of a reassortant field strain (IND2014/01) of Bluetongue virus (BTV) serotype 16, isolated from sheep from southern India in 2014, was sequenced. The total genome size was 19,186 bp. Sequence comparisons of all genome segments, except segment 5 (Seg-5), showed that IND2014/01 belonged to the major eastern topotype of BTV.